• 한국산업보건학회지
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• 제3권1호
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• pp.3-13
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• 1993

This study was conducted at seven degreasing processes in plating plants located in Seoul, Incheon, Ansan, and Taejeon areas from July 21 to august 27, 1992. This study was performed to assess the TWA exposures to trichloroethylene (TCE) and evaluate factors affecting TCE concentrations in degreasing process. Two-Point Eddy Diffusional Model suggested by Wadden et al. was employed to calculalte emission factors according to degreaser type. Results are summarized as follows. 1. The TWA exposures of the degreasing operators ranged from 1.4 ppm to 123 ppm, and those of three plants out of seven were exceeding 50 ppm of both the Korean and U.S. OSHA standards. Degreasing assistant of Plant B, was exposed to 59 ppm. 2. The average concentrations at the distance 0, 1.5, 3.0 m from the degreasers were 1.014, 24, and 18 ppm, respectively, and showed a signifficant difference by distance (p<0.01). 3. The emission of TCE was reduced by installing local exhaust systems, condensers, and refrigeration lines at the degreasers (p<0.01). 4. The major factors related to exposure of operators were workload (r=.9621. p<0.01) and dimensions of degreansing room(r=-.8667, p<0.05). 5. If the air in degreasing room is mixed violently by other factors in addition to diffusion, the emission factors can not be evaluated because the important hypothesis of the Two-Point Eddy Diffusional Model can not be accepted. 6. The ultrasonic degreaser without the local exhaust ststem, condenser, and refrigeration lines emitted TCE three times greater than the ultrasonic degreaser with condenser and refrigeration 1ines only.

• Journal of Korean Neurosurgical Society
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• 제49권6호
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• pp.345-350
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• 2011

Objective : It is debatable whether an anterior oblique fracture orientation is really a contraindication to anterior odontoid screw fixation. The purpose of this study was to investigate the feasibility of anterior odontoid screw fixation of type II and rostral shallow type III fracture with an anterior oblique fracture orientation. Methods : The authors evaluated 16 patients with type II and rostral shallow type III odontoid fracture with an anterior oblique fracture orientation. Of these 16 patients, 8 (group 1) were treated by anterior odontoid screw fixation, and 8 (group 2) by a posterior C1-2 arthrodesis. Results : Of the 8 patients in group 1, seven patients achieved solid bone fusion (87.5%), and one experienced screw back-out of the C-2 body two months after anterior screw fixation. All patients treated by posterior C1-C2 fusion in group 2 achieved successful bone fusion. Mean fracture displacements and fracture gaps were not significantly different in two groups. (p=0.075 and 0.782). However, mean fracture orientation angles were $15.3{\pm}3.2$ degrees in group 1, and $28.6{\pm}8.1$ degrees in group 2 (p=0.002), and mean fragment angulations were $3.2{\pm}2.1$ degrees in group 1, and $14.8{\pm}3.7$ degrees in group 2 (p=0.001). Conclusion : Even when the fracture lines of type II and rostral shallow type III fractures are oriented in an anterior oblique direction, anterior odontoid screw fixation can be feasible in carefully selected patients with a relatively small fracture orientation angle and relatively small fragment angulation.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2002년도 춘계학술대회
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• pp.91-97
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• 2002

In this thesis presents line balancing problems of two-sided and mixed model assembly line widely used in practical fields using genetic algorithm for reducing throughput time, cost of tools and fixtures and improving flexibility of assembly lines. Two-sided and mixed model assembly line is a special type of production line where variety of product similar in product characteristics are assembled in both sides. This thesis proposes the genetic algorithm adequate to each step in tow-sided and mixed model assembly line with suitable presentation, individual, evaluation function, selection and genetic parameter. To confirm proposed genetic algorithm, we apply to increase the number of tasks in case study. And for evaluation the performance of proposed genetic algorithm, we compare to existing algorithm of one-sided and mixed model assembly line. The results show that the algorithm is outstanding in the problems with a larger number of stations or larger number of tasks.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.92-92
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• 1993

Point mutations in a highly conserved central region of the HIV-1 integrase protein were analyzed for their effects on viral replication and virion morphogenesis. Conservative amino acid replacements of two amino acid residues invariant un retroviral integrases, D116 and E152 of HIV-1, as well as the highly conserved amino acid S147, completely blocked viral replication in two CD4$\^$＋/ human T cell lines. Mutation of four other highly conserved amino acids in the region had no detectable effect on viral replication, while Mutations at two positions, N117 and Y143, resulted in viruses with a delayed replication phenotype. Characteristic and reproducible defects id virion core structure were observed by electron microscopic analysis of sore of the replication defective integrase point mutants, indicating that mutant integrase proteins can interfere with the process of virion core maturation.

• 대한안전경영과학회지
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• 제4권2호
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• pp.83-101
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• 2002

In this thesis presents line balancing problems of two-sided and mixed model assembly line widely used in practical fields using genetic algorithm for reducing throughput time, cost of tools and fixtures and improving flexibility of assembly lines. Two-sided and mixed model assembly line is a special type of production line where variety of product similar in product characteristics are assembled in both sides. This thesis proposes the genetic algorithm adequate to each step in tow-sided and mixed model assembly line with suitable presentation, individual, evaluation function, selection and genetic parameter. To confirm proposed genetic algorithm, we apply to increase the number of tasks in case study. And for evaluation the performance of proposed genetic algorithm, we compare to existing algorithm of one-sided and mixed model assembly line. The results show that the algorithm is outstanding in the problems with a larger number of stations or larger number of tasks.

• 대한인간공학회지
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• 제36권4호
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• pp.343-353
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• 2017

Objective: This study aims to evaluate the efficiency of two representative subway map design types, namely a diagram type and a geographical type using physiological metrics, performance metrics, and self-reported metrics, which are representative usability metrics. Background: Subway maps need to be designed in order for users to quickly search and recognize subway line information. Although most cities' subway maps currently use the diagram type designed by Henry Beck, New York City's subway map has recently been changed to the subway map type combined with the geographical type designed by Michael Hertz. However, not many studies on its efficiency are found, and the studies that are available mainly depend on questionnaire surveys or take on a subjective behavioral study type based on experts' experiences. In this regard, evaluation through a more objective method is needed. Method: This study employed usability metrics as a method to evaluate the efficiency of information search targeting the diagram type and geographical type subway maps used mostly as subway maps. To this end, physiological metrics obtained through eye tracking, task completion time, representative metric of task performance, and subjective evaluation metrics were used for the suitability evaluation of subway map designs. Results: In the result of gaze movement distance analysis, no significant difference was shown in the two design types in terms of a process finding a departure station from the starting point and a process finding a transfer station between the departure station and arrival station (destination). However, the gaze movement distance in the process finding the arrival station at the departure station was significantly shorter in the geographical type, rather than in the diagram type. The analysis of task completion time showed a result similar to the gaze movement distance analysis result. Task completion time was significantly shorter in the geographical type, rather than in the diagram type, which is in the process finding the arrival station at the departure station. In other information search processes, no significant difference was shown. As a result of subjective evaluation metrics analysis, no significant difference was revealed in the two design types. Conclusion: An analysis on the two representative subway map design types was carried out via the adoption of usability metrics. As a result, although no significant difference was shown in some information search processes, it was revealed that information search was easier in the geographical type overall. Also, it was found that usability metrics can be effectively used to evaluate the design types of subway maps. Application: The study results can be used to set design direction to offer ease in information search on subway lines. The study also can be used as a method to evaluate a subway map's design type.

• 대한약리학회지
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• 제29권1호
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• pp.139-148
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• 1993

SCC 12와 SCC 13 세포주는 피부의 상피암 조직에서 유래되었다. 본 연구에서는, 세포배양으로 in vivo 피부의 거의 모든 특징들을 재현할 수 있는 raft 배양법을 이용하여, 두 종류의 상피 암세포주의 진피 침투성 정도를 비교해 보았다. SCC 12 세포주의 raft 배양에서는, collagen matrix로 침투하는 많은 세포군들이 관찰되었고, 또한 기저세포와 유사한 세포들이 분화층 중간에 관찰되었다. 그러나 SCC 13 세포주의 raft 배양에서는 침투성 세포들이 전혀 관찰되지 않았다. 어떤 인자가 SCC 12 세포주의 침투성에 관여하였는지를 조사하기 위하여, 세포이동 또는 matrix 파괴에 관여하는 각종 단백질 즉, type 1 collagenase, Fn, Fn 수용체와 EGF 수용체 등의 기본 생합성속도를 침투성과 비침투성 세포주에서 측정하였다. 그 중 type 1 collagenase의 생합성만이 침투성 세포주에서 현저히 높게 나타났다. 침투성 SCC 12 세포주에서 관찰된 높은 type 1 collagenase 생합성과 이들 세포주의 침투성과의 연관관계 및 그 기작을 조사하기 위하여 두 세포주에서 TGF alpha에 대한 반응의 차이를 조사하였다. 비침투성 세포주에서는, TGF alpha에 의해 type 1 collagenase의 생합성이 처음 12시간 동안에 현저한 증가를 보였으나 24시간부터 그 유도가 down-regulation되는 현상을 보였다. 그러나 침투성 세포는 TGF alpha에 의해 계속적인 type 1 collagenase의 유도를 보였다. 이에 저자들은 EGF 수용체의 down-regulation으로 인해 type 1 collagenase의 down-regulation이 유도되고, 이와 같은 regulation의 결함으로 인해 SCC 12 세포주가 collagen matrix로 침투하는 현상을 나타낼 것으로 제시하였다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.95-105
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• 2005

Transposon-mediated insertional mutagenesis provides one of the most powerful tools for functional studies of genes in higher plants. This project has been performed to develop a large population of insertional mutations, and to construct databases of molecular information on Ds insertion sites in rice. Ultimate goals are to supply genetic materials and information to analyze gene function and to identify and utilize agronomically important genes for breeding purpose. Two strategies have been employed to generate the large scale of transposon population in a Japonica type rice, Dongjin Byeo; 1) genetic crosses between Ac and Ds lines and 2) plant regeneration from seeds carrying Ac and Ds. Our study showed that over 70% of regenerated plants generally carried independent Ds elements and high activity of transposition was detected only during regeneration period. Ds-flanking DNA amplified from leaf tissues of F2 and T1 (or T2) plants have been amplified via TAIL-PCR and directly sequenced. So far, over 65,000 Ds lines have been generated and over 9,500 Ds loci have been mapped on chromosomes by sequence analysis. Database of molecular information on Ds insertion sites has been constructed, and has been opened to the public and will be updated soon at http://www.niab.go.kr. Detailed functional analysis of more than 30 rice mutants has been performed. Several Ds-tagged rice genes that have been selected for functional analysis will be briefly introduced. We expect that a great deal of information and genetic resources of Ds lines would be obtained during the course of this project, which will be shared with domestic and international rice researchers. In addition to the Japonica rice, we have established the tagging system in an rice line of indica genetic background, MGRI079. MGRI079 (Indica/Japonica) was transformed with Agrobacteria carrying Ac and Ds T-DNA vectors. Among transgenic lines, we successfully identified single-copy Ds and Ac lines in MGR1079. These lines were served as ‘starter lines’ to mutagenize Indica genetic background. To achieve rapid, large scale generation of Ds transposant lines, MGR1079 transformants carrying homozygous Ac were crossed with ones with homozygous Ds, and $F_2$seeds were used for plant regeneration. In this year, over 2,000 regeneration plants were grown in the field. We are able to evaluate the tagging efficiency in the Indica genetic background in the fall.

• Molecules and Cells
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• 제25권1호
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• pp.20-29
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• 2008

Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6047-6053
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• 2012

Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations ($200{\mu}g/mL$) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and $30{\mu}g/mL$) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.