• Title/Summary/Keyword: Two layer

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Effect of layer combinations with nanocomposite and low-shrinkage composite resins on their color and mechanical properties (나노복합레진과 저수축 복합레진의 복합 층으로 이룬 시편이 색과 물리적 성질에 미치는 영향)

  • Park, Wan-Ky;Choi, An-na;Son, Sung-Ae;Kwon, Yong Hoon;Kang, Eun-Sook;Park, Jeong-Kil
    • Korean Journal of Dental Materials
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    • v.44 no.2
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    • pp.129-139
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    • 2017
  • This study investigated the colors and mechanical properties of layered dental composites. Four nanocomposite resins (Aelite LS, Grandio, Tetric EvoCeram, Filtek Z350XT) and a silorane-based composite resin (P90) were used for overlying and underlying materials, respectively, with different thickness combinations. Colors, translucency parameter (TP), flexural and compressive properties were evaluated. All tested specimens had different color coordinates, although all were of A3 shade. Color coordinates and TP values of layered specimens better matched those of the corresponding overlying product as the thickness of the overlying product was increased. High TP values were related with high $b^*$ value differences between specimens (p<0.05). Both flexural strength and modulus, compressive strength and modulus of layered specimens with different thickness combinations were mostly lower than those of the corresponding overlying products, respectively, in their non-layered state.

Comparison of the Effects of Mg-Sulfate and Mg-Hydroxide on Soil pH, EC and Exchangeable Cation Distribution (황산고토와 수산화고토 비료가 토양 pH, EC 및 교환성 양이온 분포에 미치는 영향 비교)

  • Lee, Sang-Jo;Lee, Sung-Ho;Shin, Hyun-Jin;Cho, Hyun-Jong;Kim, Bok-Jin;Chung, Jong-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.3
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    • pp.105-112
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    • 2003
  • Effects of magnesium sulfate ($MgSO_4$) and magnesium hydroxide $[Mg(OH)_2]$, which have different chemical characteristics, on soil pH, electrical conductivity (EC), and exchangeable cation distributions were investigated. Using plastic columns packed with a loam soil, the two Mg-fertilizers were treated at the rate of $300kg\;MgO\;ha^{-1}$ and water was applied on the soil surface four times at every 7 days. Soil samples were taken at 5, 10, 15, and 20 cm depth after 7 days of each water application. Magnesium hydroxide could increase soil pH, but due to the low solubility of $Mg(OH)_2$, the effect on pH was limited on the surface soil. Soil pH was lowered in the $MgSO_4$ treatment and the effect was found through the 20 cm depth. Since the pH decrease in $MgSO_4$ treated soil was due to the salts from $MgSO_4$, after leaching of most salts from the investigated soil depth pH was not significantly different from that of non-treated soil. Soil EC was increased in $MgSO_4$ treatment through the soil depth, but in $Mg(OH)_2$ treatment EC was slightly increased only at the surface layer. Exchangeable Mg was increased in both of the treatments at surface layer after the first water application. In $Mg(OH)_2$ treatment, the increase of exchangeable Mg was found only at 5 cm depth through the experiment, but leaching down of Mg in the $MgSO_4$ treatment was very apparent. High concentration of Mg in the $MgSO_4$ treated soil could effectively replace exchangeable Ca through the investigated soil depth, but the effect of $Mg(OH)_2$ on exchangeable Ca was not significant.

Label Embedding for Improving Classification Accuracy UsingAutoEncoderwithSkip-Connections (다중 레이블 분류의 정확도 향상을 위한 스킵 연결 오토인코더 기반 레이블 임베딩 방법론)

  • Kim, Museong;Kim, Namgyu
    • Journal of Intelligence and Information Systems
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    • v.27 no.3
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    • pp.175-197
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    • 2021
  • Recently, with the development of deep learning technology, research on unstructured data analysis is being actively conducted, and it is showing remarkable results in various fields such as classification, summary, and generation. Among various text analysis fields, text classification is the most widely used technology in academia and industry. Text classification includes binary class classification with one label among two classes, multi-class classification with one label among several classes, and multi-label classification with multiple labels among several classes. In particular, multi-label classification requires a different training method from binary class classification and multi-class classification because of the characteristic of having multiple labels. In addition, since the number of labels to be predicted increases as the number of labels and classes increases, there is a limitation in that performance improvement is difficult due to an increase in prediction difficulty. To overcome these limitations, (i) compressing the initially given high-dimensional label space into a low-dimensional latent label space, (ii) after performing training to predict the compressed label, (iii) restoring the predicted label to the high-dimensional original label space, research on label embedding is being actively conducted. Typical label embedding techniques include Principal Label Space Transformation (PLST), Multi-Label Classification via Boolean Matrix Decomposition (MLC-BMaD), and Bayesian Multi-Label Compressed Sensing (BML-CS). However, since these techniques consider only the linear relationship between labels or compress the labels by random transformation, it is difficult to understand the non-linear relationship between labels, so there is a limitation in that it is not possible to create a latent label space sufficiently containing the information of the original label. Recently, there have been increasing attempts to improve performance by applying deep learning technology to label embedding. Label embedding using an autoencoder, a deep learning model that is effective for data compression and restoration, is representative. However, the traditional autoencoder-based label embedding has a limitation in that a large amount of information loss occurs when compressing a high-dimensional label space having a myriad of classes into a low-dimensional latent label space. This can be found in the gradient loss problem that occurs in the backpropagation process of learning. To solve this problem, skip connection was devised, and by adding the input of the layer to the output to prevent gradient loss during backpropagation, efficient learning is possible even when the layer is deep. Skip connection is mainly used for image feature extraction in convolutional neural networks, but studies using skip connection in autoencoder or label embedding process are still lacking. Therefore, in this study, we propose an autoencoder-based label embedding methodology in which skip connections are added to each of the encoder and decoder to form a low-dimensional latent label space that reflects the information of the high-dimensional label space well. In addition, the proposed methodology was applied to actual paper keywords to derive the high-dimensional keyword label space and the low-dimensional latent label space. Using this, we conducted an experiment to predict the compressed keyword vector existing in the latent label space from the paper abstract and to evaluate the multi-label classification by restoring the predicted keyword vector back to the original label space. As a result, the accuracy, precision, recall, and F1 score used as performance indicators showed far superior performance in multi-label classification based on the proposed methodology compared to traditional multi-label classification methods. This can be seen that the low-dimensional latent label space derived through the proposed methodology well reflected the information of the high-dimensional label space, which ultimately led to the improvement of the performance of the multi-label classification itself. In addition, the utility of the proposed methodology was identified by comparing the performance of the proposed methodology according to the domain characteristics and the number of dimensions of the latent label space.

A Protocol of Ludox Treatment for Physiological and Molecular Biological Research of Freshwater Cyanobacteria (퇴적층 남조류 휴면세포의 생리적-분자생물학적 연구를 위한 Ludox 처리법)

  • Keonhee Kim;Kyeong-eun Yoo;Hye-in Ho;Chaehong Park;Hyunjin Kim;Soon-Jin Hwang
    • Korean Journal of Ecology and Environment
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    • v.56 no.1
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    • pp.94-103
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    • 2023
  • Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria's early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

Comparison of Two Methods for Estimating the Appearance Probability of Seawater Temperature Difference for the Development of Ocean Thermal Energy (해양온도차에너지 개발을 위한 해수온도차 출현확률 산정 방법 비교)

  • Yoon, Dong-Young;Choi, Hyun-Woo;Lee, Kwang-Soo;Park, Jin-Soon;Kim, Kye-Hyun
    • Journal of the Korean Association of Geographic Information Studies
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    • v.13 no.2
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    • pp.94-106
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    • 2010
  • Understanding of the amount of energy resources and site selection are required prior to develop Ocean Thermal Energy (OTE). It is necessary to calculate the appearance probability of difference of seawater temperature(${\Delta}T$) between sea surface layer and underwater layers. This research mainly aimed to calculate the appearance probability of ${\Delta}T$ using frequency analysis(FA) and harmonic analysis(HA), and compare the advantages and weaknesses of those methods which has used in the South Sea of Korea. Spatial scale for comparison of two methods was divided into local and global scales related to the estimation of energy resources amount and site selection. In global scale, the Probability Differences(PD) of calculated ${\Delta}T$ from using both methods were created as spatial distribution maps, and compared areas of PD. In local scale, both methods were compared with not only the results of PD at the region of highest probability but also bimonthly probabilities in the regions of highest and lowest PD. Basically, the strong relationship(pearson r=0.96, ${\alpha}$=0.05) between probabilities of two methods showed the usefulness of both methods. In global scale, the area of PD more than 10% was less than 5% of the whole area, which means both methods can be applied to estimate the amount of OTE resources. However, in practice, HA method was considered as a more pragmatic method due to its capability of calculating under various ${\Delta}T$ conditions. In local scale, there was no significant difference between the high probability areas by both methods, showing difference under 5%. However, while FA could detect the whole range of probability, HA had a disadvantage of inability of detecting probability less than 10%. Therefore it was analyzed that the HA is more suitable to estimate the amount of energy resources, and FA is more suitable to select the site for OTE development.

Phytochemical Analysis and Anti-cancer Investigation of Boswellia Serrata Bioactive Constituents In Vitro

  • Ahmed, Hanaa H;Abd-Rabou, Ahmed A;Hassan, Amal Z;Kotob, Soheir E
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.16
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    • pp.7179-7188
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    • 2015
  • Cancer is a major health obstacle around the world, with hepatocellular carcinoma (HCC) and colorectal cancer (CRC) as major causes of morbidity and mortality. Nowadays, there isgrowing interest in the therapeutic use of natural products for HCC and CRC, owing to the anticancer activity of their bioactive constituents. Boswellia serrata oleo gum resin has long been used in Ayurvedic and traditional Chinese medicine to alleviate a variety of health problems such as inflammatory and arthritic diseases. The current study aimed to identify and explore the in vitro anticancer effect of B. Serrata bioactive constituents on HepG2 and HCT 116 cell lines. Phytochemical analysis of volatile oils of B. Serrata oleo gum resin was carried out using gas chromatography-mass spectrometry (GC/MS). Oleo-gum-resin of B. Serrata was then successively extracted with petroleum ether (extract 1) and methanol (extract 2). Gas-liquid chromatography (GLC) analysis of the lipoidal matter was also performed. In addition, a methanol extract of B. Serrata oleo gum resin was phytochemically studied using column chromatography (CC) and thin layer chromatography (TLC) to obtain four fractions (I, II, III and IV). Sephadex columns were used to isolate ${\beta}$-boswellic acid and identification of the pure compound was done using UV, mass spectra, $^1H$ NMR and $^{13}C$ NMR analysis. Total extracts, fractions and volatile oils of B. Serrata oleo-gum resin were subsequently applied to HCC cells (HepG2 cell line) and CRC cells (HCT 116 cell line) to assess their cytotoxic effects. GLC analysis of the lipoidal matter resulted in identification of tricosane (75.32%) as a major compound with the presence of cholesterol, stigmasterol and ${\beta}$-sitosterol. Twenty two fatty acids were identified of which saturated fatty acids represented 25.6% and unsaturated fatty acids 74.4% of the total saponifiable fraction. GC/MS analysis of three chromatographic fractions (I,II and III) of B. Serrata oleo gum resin revealed the presence of pent-2-ene-1,4-dione, 2-methyl- levulinic acid methyl ester, 3,5- dimethyl- 1-hexane, methyl-1-methylpentadecanoate, 1,1- dimethoxy cyclohexane, 1-methoxy-4-(1-propenyl)benzene and 17a-hydroxy-17a-cyano, preg-4-en-3-one. GC/MS analysis of volatile oils of B. Serrata oleo gum resin revealed the presence of sabinene (19.11%), terpinen-4-ol (14.64%) and terpinyl acetate (13.01%) as major constituents. The anti-cancer effect of two extracts (1 and 2) and four fractions (I, II, III and IV) as well as volatile oils of B. Serrata oleo gum resin on HepG2 and HCT 116 cell lines was investigated using SRB assay. Regarding HepG2 cell line, extracts 1 and 2 elicited the most pronounced cytotoxic activity with $IC_{50}$ values equal 1.58 and $5.82{\mu}g/mL$ at 48 h, respectively which were comparable to doxorubicin with an $IC_{50}$ equal $4.68{\mu}g/mL$ at 48 h. With respect to HCT 116 cells, extracts 1 and 2 exhibited the most obvious cytotoxic effect; with $IC_{50}$ values equal 0.12 and $6.59{\mu}g/mL$ at 48 h, respectively which were comparable to 5-fluorouracil with an $IC_{50}$ equal $3.43{\mu}g/mL$ at 48 h. In conclusion, total extracts, fractions and volatile oils of B. Serrata oleo gum resin proved their usefulness as cytotoxic mediators against HepG2 and HCT 116 cell lines with different potentiality (extracts > fractions > volatile oil). In the two studied cell lines the cytotoxic acivity of each of extract 1 and 2 was comparable to doxorubicin and 5-fluorouracil, respectively. Extensive in vivo research is warranted to explore the precise molecular mechanisms of these bioactive natural products in cytotoxicity against HCC and CRC cells.

The nanoleakage patterns of experimental hydrophobic adhesives after load cycling (Load cycling에 따른 소수성 실험용 상아질 접착제의 nanoleakage 양상)

  • Sohn, Suh-Jin;Chang, Ju-Hae;Kang, Suk-Ho;Yoo, Hyun-Mi;Cho, Byeong-Hoon;Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.33 no.1
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    • pp.9-19
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    • 2008
  • The purpose of this study was: (1) to compare nanoleakage patterns of a conventional 3-step etch and rinse adhesive system and two experimental hydrophobic adhesive systems and (2) to investigate the change of the nanoleakage patterns after load cycling. Two kinds of hydrophobic experimental adhesives, ethanol containing adhesive (EA) and methanol containing adhesive (MA), were prepared. Thirty extracted human molars were embedded in resin blocks and occlusal thirds of the crowns were removed. The polished dentin surfaces were etched with a 35 % phosphoric acid etching gel and rinsed with water. Scotchbond Multi-Purpose (MP), EA and MA were used for bonding procedure. Z-250 composite resin was built-up on the adhesive-treated surfaces. Five teeth of each dentin adhesive group were subjected to mechanical load cycling. The teeth were sectioned into 2 mm thick slabs and then stained with 50 % ammoniacal silver nitrate. Ten specimens for each group were examined under scanning electron microscope in backscattering electron mode. All photographs were analyzed using image analysis software. Three regions of each specimen were used for evaluation of the silver uptake within the hybrid layer. The area of silver deposition was calculated and expressed in gray value. Data were statistically analyzed by two-way ANOVA and post-hoc testing of multiple comparisons was done with the Scheffe's test. Silver particles were observed in all the groups. However, silver particles were more sparsely distributed in the EA group and the MA group than in the MP group (p < .0001). There were no changes in nanoleakage patterns after load cycling.

Developing Scoring Rubric and the Reliability of Elementary Science Portfolio Assessment (초등 과학과 포트폴리오의 채점기준 개발과 신뢰도 검증)

  • Kim, Chan-Jong;Choi, Mi-Aee
    • Journal of The Korean Association For Science Education
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    • v.22 no.1
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    • pp.176-189
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    • 2002
  • The purpose of the study is to develop major types of scoring rubrics of portfolio system, and estimate the reliability of the rubrics developed. The portfolio system was developed by Science Education Laboratory, Chongju National University of Education in summer, 2000. The portfolio is based on the Unit 2, The Layer and Fossil, and Unit 4, Heat and Change of Objects at fourth-grade level. Four types of scoring rubrics, holistic-general, holistic-specific, analytical-general, and analytical-specific, were developed. Students' portfolios were scored and inter-rater and intra-rater reliability were calculated. To estimate inter-rater reliability, 3 elementary teachers per each rubric(total 12) scored 12 students' portfolios. Teachers who used analytical-specific rubric scored only six portfolios because it took much more time than other rubrics. To estimate intra-rater reliability, second scoring was administered by two raters per rubric in two and half month. The results show that holistic-general rubric has high inter-rater and moderate intra-rater reliability. Holistic-specific rubric shows moderate inter- and intra-rater reliability. Analytical-general rubric has high inter-rater and moderate intra-rater reliability. Analytical-specific rubric shows high inter- and intra-rater reliability. The raters feel that general rubrics seems to be practical but not clear. Specific rubrics provide more clear guidelines for scoring but require more time and effort to develop the rubrics. Analytical-specific rubric requires more than two times of time to score each portfolio and is proved to be highly reliable but less practical.

Effect of Dietary Supplementation of Fresh Water Algae Euglena on the Performance and Egg Quality and Fatty Acid Composition of Egg Yolk in Laying Hens (산란계에서 담수녹조류 Euglena의 첨가사료가 생산성 및 계란의 품질과 지방산 조성에 미치는 영향)

  • Choi S. W.;Park I. K.;Park B. S.
    • Korean Journal of Poultry Science
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    • v.31 no.4
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    • pp.283-291
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    • 2004
  • Feeding trials were conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the laying performance, egg quality and fatty acid composition of egg yolk was studied. In experiment I, two hundred eighty 32-wk-old ISA Brown layers were randomly assigned to seven dietary treatments for 4 wks. Each treatment consisted of 4 replications with 10 birds each housed in two birds cages. Control diet was formulated to have $17\%$ CP and 2,750 kcal ME/kg. Euglena gracilis Z. (EG) was added to control diet at the level of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, three hundred 84-wk-old ISA brown layers were randomly assigned to five dietary treatments: T1; Control, T2; T1 + EGBD $0.5\%$, T3; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T4; T1 + EGD $1.0\%$, T5; T1 + EGD $2.0\%$. Each treatment had 5 replication of 12 birds each housed in two birds cages. In experiments 1 and 2, Euglena suppplementation did not significantly affect egg production but increased egg weight and feed intake. In experiment 1, EG was more effective in increasing egg yolk color score than EGBD. Egg yolk color of EG $1\%$ treatment showed the highest score. EGBD supplementation increased DHA concentration of egg yolk. EGBD $2\%$ treatment showed the highest DHA and the lowest palmitic and stearic acids concentration in the egg yolk. In experiment 2, EGBD $0.5\%$ treatment showed highest DHA level in egg yolk (P<0.05). It was conducted that EGBD is a single cell protein source rich in DHA, that can be used to produce DHA enriched eggs.

The Improvement of Real-time Updating Methods of the National Base Map Using Building Layout Drawing (건물배치도를 이용한 국가기본도 수시수정 방법 개선)

  • Shin, Chang Soo;Park, Moon Jae;Choi, Yun Soo;Baek, kyu Yeong;Kim, Jaemyeong
    • Journal of Cadastre & Land InformatiX
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    • v.48 no.1
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    • pp.139-151
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    • 2018
  • The National Base Map construction consists of the regular correction work of dividing the whole country into two regions and carrying out the modification Plotting by aerial photographs every two years as well as the real time updating work of correcting the major change feature within two weeks by the field survey and the As-Built Drawing. In the case of the Building Layout Drawing of Korea Real estate Administration intelligence System(KRAS) used for real time updating work of the National base map, the coordinate transformation error is included in the positional error when applied to the National Base Map based on the World Geodetic Reference System as the coordinate system based on the Regional Geodetic Reference System. In addition, National Base Map is registered based on the outline(eaves line) of the building in the Digital Topographic Map, and the Cadastral and Architecture are registered based on the building center line. Therefore, the Building Object management standard is inconsistent. In order to investigate the improvement method, the network RTK survey was conducted directly on a location of the Building Layout Drawing of Korea Real estate Administration intelligence System(KRAS) and the problems were analyzed by comparing with the plane plotting position reference in National Base Map. In the case of the general structure with the difference on the Building center line and the eaves line, beside the location information was different also the difference in the ratio of the building object was different between Building center line and the eave. In conclusion, it is necessary to provide the Base data of the double layer of the Building center line and the outline of the building(eaves line) in order to utilize the Building Layout Drawing of Korea Real estate Administration intelligence System(KRAS). In addition, it is necessary to study an organic map update process that can acquire the up-to-dateness and the accuracy at the same time.