• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.58-62
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• 2006

Rapid assay of enzyme is a primary requirement for successful application of directed evolution technology. Halo generation on a turbid plate would be a method of choice for high throughput screening of enzymes in this context. Here we report a new approach to prepare turbid plates, by controlling the crystallization of tyrosine to form needle-like particles. In the presence of tyrosine phenol-lyase (TPL), the needle-like tyrosine crystals were converted to soluble phenol rapidly than the usual rectangular tyrosine crystals. When an error-prone PCR library of Citrobacter freundii TPL was spread on the turbid plate, approximately 10% of the colonies displayed recognizable halos after 24 hours of incubation at $37^{\circ}C$. Representative positives from the turbid plates were transferred to LB-medium in 96-wellplates, cultivated overnight, and assayed for the enzyme activity with L-tyrosine as the substrate. The assay results were approximated to be proportional to the halo size on turbid plates, suggesting the screening system is directly applicable to the directed evolution of TPL. Actually, two best mutants on the turbid plates were identified to be $2{\sim}2.5$ and 1.5-fold improved in the activity.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.5
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• pp.13-22
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• 2018

In this study, a governing differential equation for the bending problem of orthotropic rectangular plate is drived. It's exact solution for various boundary conditions is presented. This solution follows traditional method like Navier's solution or Levy's solution that transforms the governing differential equation into an algebraic equation by using trigonometric series. To obtain a solution by Levy's method, it is required that two opposite edges of the plate be simply supported. And the boundary conditions, for which the Navier's method is applicable, are simply supported edge at all edges. In this study, it overcomes the limitations of the previous Navier's and Levy's methods.This solution is applicable for any combination of boundary conditions with simply supported edge and clamped edge in x, y direction. The plate could be subjected to uniform, partially uniform, and line loads. The advantage of the solution is that it is the exact solution as well as it overcomes the limitations of the previous Navier's and Levy's methods. Calculations are presented for orthotropic plates with nonsymmetric boundary conditions. Comparisons between the result of this paper and the result of Navier, Levy and Szilard solutions are made for the isotropic plates. The deflections were in excellent agreement.

• Steel and Composite Structures
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• v.15 no.2
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• pp.203-220
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• 2013

The study of the tensile strength of composite materials is far more complex than analysis of the properties of elasticity and plasticity. Indeed, during mechanical loading, micro-cracks in the matrix, the fibers break, debonding of the interfaces are created. The failure process of composites is of great diversity and cannot be described if even we know: the strength criterion of each individual component, the state of stress and strain in the material, the propagation phenomena cracks in the structure and nature of the interface between the matrix and the reinforcement. This information is only partially known and the obtained by the analysis of a stress limit beyond which there is destruction of the material is almost impossible. To partially process the issue, a solution lies in a mesoscopic approach of seeking a law to locate the ultimate strength of the material for a plane stress state. Tests on rectangular plates in bending PEEK/APC2 and T300/914 three were made and this in order to validate our approach, the calculation has been implemented in a nonlinear finite element code (Castem 2000), in order to make comparison with the numerical results. The results show good agreement between numerical simulation and the two materials; however, it would be interesting to consider other phenomena in the criterion.

• Earthquakes and Structures
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• v.14 no.2
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• pp.117-128
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• 2018

In this research work, free vibrations of simply supported functionally graded plate resting on a Winkler-Pasternak elastic foundation are investigated by a new shear deformation theory. The influence of alternative micromechanical models on the macroscopic behavior of a functionally graded plate based on shear-deformation plate theories is examined. Several micromechanical models are tested to obtain the effective material properties of a two-phase particle composite as a function of the volume fraction of particles which continuously varies through the thickness of a functionally graded plate. Present theory exactly satisfies stress boundary conditions on the top and the bottom of the plate. The energy functional of the system is obtained using Hamilton's principle. The closed form solutions are obtained by using Navier technique, and then fundamental frequencies are found by solving the results of eigenvalue problems. Finally, the numerical results are provided to reveal the effect of explicit micromechanical models on natural fundamental frequencies.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.4
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• pp.507-514
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• 2003

Most of CAE analyses for injection molding have been based on the Mele Shaw's approximation: two-dimensional flow analysis. in some cases, that approximation causes significant errors due to loss of the geometrical information as well as simplification of the flow characteristics in the thickness direction. Although injection molding analysis software using three-dimensional solid elements has been developed recently, such as Moldflow Flow3D, it does not contain a deformation analysis function yet. The present work covers three-dimensional deformation analysis or injection molded plastic parts using solid elements. A numerical scheme for deformation analysis has bun proposed from the results of injection molding analysis using Moldflow Flow3D. The accuracy of the proposed approach has been verified through a numerical analysis of rectangular plates with various thicknesses in comparison with the classical shell-based approach. In addition, the reliability of the approach has also been proved through an industrial example. an optical plastic lens, in comparison of real experiments.

• Journal of Power Electronics
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• v.16 no.5
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• pp.1678-1688
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• 2016

This paper presents a capacitive power transfer (CPT) system using a Class-E resonant inverter. A Class-E resonant inverter is chosen because of its ability to perform DC-to-AC inversion efficiently while significantly reducing switching losses. The proposed CPT system consists of an efficient Class-E resonant inverter and capacitive coupling formed by two flat rectangular transmitter and receiver plates. To understand CPT behavior, we study the effects of various coupling distances on output power performance. The proposed design is verified through lab experiments with a nominal operating frequency of 1 MHz and 0.25 mm coupling gap. An efficiency of 96.3% is achieved. A simple frequency tracking unit is also proposed to tune the operating frequency in response to changes in the coupling gap. With this resonant frequency tracking unit, the efficiency of the proposed CPT system can be maintained within 96.3%-91% for the coupling gap range of 0.25-2 mm.

• Journal of Mechanical Science and Technology
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• v.15 no.1
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• pp.98-107
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• 2001

Continuing efforts to achieve increased circuit performance in electronic package have resulted in higher power density at chip and module level. As a result, the thermal management of electronic package has been important in maintaining or improving the reliability of the component. An experimental investigation of thermosyphonic boiling in vertical tube and channel made by two parallel rectangular plates was carried out in this study for possible application of the direct immersion cooling. Fluorinert FC-72 as a working fluid was used in this experiment. Asymmetric heated channel of open periphery with gap size of 1, 2, 4 and 26mm and uniformly heated vertical tubes with diameter of 9, 15 and 20mm were boiled at saturated condition. The boiling curves from tested surfaces exhibited the boiling hysteresis. It was also found that the gap size is not a significant parameter for the thermosyphonic boiling heat transfer with this Fluorinert. Rather pool boiling characteristics appeared for larger gap size and tube diameter. The heat transfer coefficients measured were also compared with the calculation results by Chens correlation.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.29 no.2
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• pp.84-89
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• 2016

We present the results of a study of a piezoelectric generator that generates electricity by the application of tension to an element. A device is named "EYE-type generator". The EYE-type generator consists of a rectangular ceramic and two elastic body plates that are attached to upper and lower surfaces of a ceramic. If tension is applied to both ends of the elastic body, that tension is transformed to pressure on the ceramic through a change in the form of the elastic body, causing a piezoelectric effect whereby electricity is generated by the ceramic. This generator is relatively durable because a forces are not applied directly to the ceramic. We examined dependencies of the generator's output characteristics on the size of the ceramic and elastic body. A resonance and output characteristics were analyzed by using a finite element method. The generator was fabricated based on results of the analysis, and this was attached to a frequency-controllable vibrator to measure the output characteristics. The measured results were compared with results of the simulation, and the results pointed to the practicality of the design.

• Structural Engineering and Mechanics
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• v.40 no.1
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• pp.13-28
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• 2011

An effective method for detection linear flaws in plate structures via two-dimensional discrete wavelet transform is proposed in this study. The proposed method was applied to a four-fixed supported rectangular plate containing damage with arbitrary length, depth and location. Numerical results identifying the damage location are compared with the actual results to demonstrate the effectiveness of the proposed method. Also, a wavelet-based method presented for de-noising of mode shape of plate. Finally, the performance of the proposed method for de-noising and damage identification was verified using experimental data. Comparison between the location detected by the proposed method, and the plate's actual damage location revealed that the methodology can be used as an accessible and effective technique for damage identification of actual plate structures.

• Advances in nano research
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• v.5 no.4
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• pp.337-357
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• 2017

Knowledge of thin films mechanical properties is strongly associated to the reliability and the performances of Nano Electro Mechanical Systems (NEMS). In the literature, there are several methods for micro materials characterization. Bulge test is an established nondestructive technique for studying the mechanical properties of thin films. This study improve the performances of NEMS by investigating the mechanical behavior of Nano rectangular thin film (NRTF) made of new material embedded in Nano Electro Mechanical Systems (NEMS) by developing the bulge test technique. The NRTF built from adhesively-bonded layers of basalt fiber reinforced polymer (BFRP) laminate composite materials in Nano size at room temperature and were used for plane-strain bulging. The NRTF is first pre-stressed to ensure that is no initial deflection before applied the loads on NRTF and then clamped between two plates. A differential pressure is applying to a deformation of the laminated composite NRTF. This makes the plane-strain bulge test idea for studying the mechanical behavior of laminated composite NRTF in both the elastic and plastic regimes. An exact solution of governing equations for symmetric cross-ply BFRP laminated composite NRTF was established with taking in-to account the effect of the residual strength from pre-stressed loading. The stress-strain relationship of the BFRP laminated composite NRTF was determined by hydraulic bulging test. The NRTF thickness gradation in different points of hemisphere formed in bulge test was analysed.