• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.77-81
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• 2006

As a result of cytotoxic compounds against cancer cell lines from natural sources, senven compounds were isolated from the leaf and twig of Acer okamotoanum Nakai. The compounds (1-7) were identified as ethyl gallate (1), methyl gallate (2), gallic acid (3), trans $resveratrol-3-O-{\beta}-D-glucopyranoside$ (4), acertannin (5), nikoenoside (6), and fraxin (7) by physicochemical and spectroscopic data (including mp, UV, IR, MS, $^1H-NMR,\;^{13}C-NMR$, DEPT, and HMBC) in comparison with those of published papers. All the compounds were tested for their cytotoxic activity against L1210, HL-60, K562, and B16F10 cancer cell lines in vitro by MTT assay method. Compounds 1-3 and 5 showed cytotoxic activity against all tested cell lines with $IC_{50}$ values ranged from 12.5 to $72.2\;{\mu}M$. Of the compounds, methyl gallate (2) exhibited the most potent cytotoxic activity against L1210, HL-60, K562, and B16F10 tumor cells with $IC_{50}$ values of 12.5, 48.3, 22.8, and $22.8\;{\mu}M$, respectively. Other compounds did not show any cytotoxic activity against four cancer cell lines.

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.382-387
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• 2009

Enhanced formation and accumulation of advanced glycation end products (AGEs) have been implicated as a major pathogenesis process leading to diabetic complications, normal aging, atherosclerosis and Alzheimer's disease. In our ongoing project to discover novel treatments for diabetic complications from natural sources, we have investigated on the inhibitory activity of 67 ethanol extracts from 57 Korean herbal medicines against the formation of AGEs in vitro. Of these, 22 extracts were found to have a significant AGEs inhibitory activity ($IC_{50}$<50 ${\mu}g$/ml) compared with aminoguanidine ($IC_{50}$=75.98 ${\mu}g$/ml). Particularly, 6 extracts from 3 herbal medicines, Castanea crenata (flower, leaf, bark-twig), Acer tatarium subsp. ginnala (fruit) and Sapium japonicum (leaf, twig) showed (approximately 8-17 fold) stronger inhibitory activity than that of aminoguanidine.

• Journal of KIISE:Databases
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• v.35 no.4
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• pp.368-378
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• 2008

In recent years, publish-subscribe (pub-sub) systems based on XML document filtering have received much attention. In a typical pub-sub system, subscribed users specify their interest in profiles expressed in the XPath language, and each new content is matched against the user profiles so that the content is delivered to only the interested subscribers. As the number of subscribed users and their profiles can grow very large, the scalability of the system is critical to the success of pub-sub services. In this paper, we propose a fast and scalable XML filtering system called SFiST which is an extension of the FiST system. Sharable segments are extracted from twig patterns and stored into the hash-based Segment Table in SFiST system. Segments are used to represent user profiles as Terse Sequences and stored in the Compact Segment Index during filtering. Our experimental study shows that SFiST system has better performance than FiST system in terms of filtering time and memory usage.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.28-35
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• 2001

To investigate the effects of nitrogen fertilization and thinning on growth and nutrition of current-year needle and twigs in Pinus koraiensis we treated two clone banks with three levels of nitrogen fertilizer in Yongin and Chuncheon, and also thinned two plantations with three intensities in Yangpyeong. The fertilization and thinning effects greatly varied depending on study sites and stand ages. However, dry weights of needle and twig increased following low nitrogen fertilization in older stands. Moderate thinning increased dry weight and nitrogen concentration of twigs in the 12-year-old stand.

• Mycobiology
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• v.50 no.2
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• pp.121-131
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• 2022

The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome. The results were assembled de novo, and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase (ACS2 and ACS4), hydroxymethylglutaryl CoA reductase (HMGR), farnesyl transferase (FTase), and squalene synthase (SQS) were found to be upregulated in XZJ (twig of C. camphora) and NZJ (twig of C. kanehirae). Moreover, ACS5 and 2,3-oxidized squalene cyclase (OCS) were highly expressed in NZJ, while heme IX farnesyl transferase (IX-FIT) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS, and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.69-76
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• 2002

Mustard aphid, Lipaphis erysimi(Kalt.) plays a key role in determining the productivity of rapeseed-mustard. This pest alone attributes 30-70% losses in yield potential in different agro climatic zones with a mean loss of 54.2% in India. Considering the extent of damage caused by the pest, the present experiment was conducted at Pulses and Oilseeds Research Station during 1992-93 to 1994-95 to study the migratory nature of mustard aphid by trapping them in yellow colour pan tray and their simultaneous growth and development on three different genotypes. The temperature during noon hours is the predominant factor to govern the appearance of alate mustard aphid in rapeseed-mustard field. Stepwise regression analysis revealed that temperature and relative humidity played an important role for its development. The variety RW white flower glossy stem harboured minimum number of aphid in comparison to other two varieties B 9 and T6342. The population reached a peak of 61.28 aphids/10 cm central twig during $6^{th}$ standard week irrespective of varieties.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1997.04a
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• pp.118-118
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• 1997

갑상피열근이나 피열근에 대하여 상후두신경과 반회후두신경의 이중지배(dual supply)는 오랜동안 논란이 되어왔다. 특히 피열근에 분포하는 신경은 반회후두신경의 피열분지에 의해 지배되며 또한 상후두 신경의 내지의 일부인 ramus preforantes에 의해서도 이중지배를 받는다고 알려져왔다. 그러나 한편 이러한 피열근의 운동신경지배 중 상후두신경의 역할에 대해서 적지않은 보고가 후두의 신경해부학적인 고찰 및 전기적 자극을 통한 연구에서 단지 피열근 근처에서 twig을 형성할 뿐, 직접적인 운동지배 현상은 보이지않음을 입증하여 피열근은 오직 반회후두신경에 의해서만 실질적으로 운동지배된다고 주장하였다. 저자는 양측 반회후두신경을 절단한 개 모델에서 피열근에 대하여 HRP 역행성 착색법 후 뇌간에서 살펴본 결과 후두의 운동신경원인의 핵에서 양성으로 표현되는 것을 관찰하였다. 또한 상후두신경의 내지에 대하여 운동신경섬유의 표식자로 여겨질 수 있는 Cholineacetyl transferase(CHAT) 면역염색을 시행한 결과 피열근의 운동신경에 상후두신경이 괸여함을 알 수 있었다.

• KSBB Journal
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• v.8 no.2
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• pp.122-125
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• 1993

Taxol is the promising diterpene alkaloid with antineoplastic activities. The taxol content in various parts of yew trees in Korea, Mt. Deokyu, Mt. Sobaek, Mt. Taebaek and Suwon area, have been determined. The highest taxol content was observed in yew trees of Mt. Soback. In various parts of trees the taxol content in bark was highest and the taxol content in twig was higher than that in leaves.

• CELLMED
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• v.2 no.4
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• pp.30.1-30.4
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• 2012

Traditional Chinese medicine classifies peripheral nerve impairment as paralysis and arthromyodynia, and considers that it is the result of defects of meridians and vessels, QI and blood, bones and muscles. Huangqi (Astragalus) Guizhi (Cassia Twig) Wuwu Tang, as a Qi invigorating formula, is usually used to improve peripheral nerve impairment. In recent years, some scholars have conducted research into Chemotherapy-induced peripheral neuropathy (CIPN) treatment with Huangqi Guizhi Wuwu Tang and certain values of this treatment approach have been identified. CIPN is a type of blood-arthralgia Zheng in traditional Chinese medicine theory. In this review, we will discuss the treatment of CIPN with Huangqi Guizhi Wuwu Tang according to blood-arthtalgia Zheng.

• Proceedings of the KSLP Conference
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• 1996.11a
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• pp.95-95
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• 1996

후두근의 운동지배신경에 관하여 일반적으로 알려진 사실은 윤상갑상근이 상후두신경의 의지에 의하여 지배되는 것 외에는 모든 후두내근이 반회후두신경에 의하여 지배된다는 것이다. 이중에서 갑상피열근이나 피열근에 대하여 상후두신경과 반회후두신경의 이중지배(dual supply)는 오랜동안 논란이 되어왔다. 특히 피열근에 분포하는 신경은 반회후두신경의 피열분지에 의하여 지배되며 또한 상후두신경의 내지의 일부인 ramus porforantes에 의해서도 이중지배를 받는다고 알려져 왔다. 그러나 한편 이러한 피열근의 운동신경지배 중 상후두신경의 역할에 대해서 적지 않은 보고가 후두의 신경해부학적인 고찰 및 전기적 자극을 통한 연구에서 단지 피열근 근처에서 twig을 형성할 뿐, 직접적인 운동지배 현상은 보이지 않음을 입증하여 피열근은 오직 반회후두신경에 의하여 운동지배된다고 주장하였다. (중략)