• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.73-78
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• 2011

This study was conducted to evaluate the effect of developed SCB (DSCB) liquid fertilizer produced by adding N, P and K at SCB liquid fertilizer on the growth of kentucky bluegrass. Two different N sources used in DSCB were ammonium sulfate (DSCB-A) and urea (DSCB-U), respectively. Fertilizer treatments were designed as follows; non-fertilizer (NF), control (CF; chemical fertilizer), DSCB-A1 ($200\;ml{\cdot}m^{-2}$DSCB-A), OSCB-A2 ($250\;ml{\cdot}m^{-2}$DSCB-A), OSCB-U ($250\;ml{\cdot}m^{-2}$DSCB-U) and CF+SCB (CF+$250\;ml{\cdot}m^{-2}$SCB). Every treatment was arranged in a randomized complete block design with three replications. In kentucky bluegrass, turf color index, chlorophyll index, dry weight and nutrient contents were measured. Results were as follows; It was hardly affected by DSCB and SCB application in investigation of chemical properties of soil. Turf color index and chlorophyll index in DSCB and SCB treatments were increased by 1~2% and 19~24% than NF, respectively and similar to CF. As applied with DSCB and SCB, dry weight of DSCB-U and CF+SCB was increased by 36% and 10% than CF, respectively, but similar to that of OSCB-A1 and DSCB-A2. Evaluated with turf quality and growth, DSCB-U was the best in all treatment and OSCB-A1 the most efficient. These results indicated that applications of OSCB and SCB promoted turf quality and growth of kentucky bluegrass or similar to CF, so that they were expected to replace chemical fertilizers.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.106-111
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• 2011

This study was conducted to investigate the effect of the mixture ratio of a soldier fly casts (SFC), compost and cocopeat on the soil physicochemical properties. The mixture ratios of soil amendment were 0%, 3%, 5%, 7% and 10% (V/V) incorporated with sand which met to the USGA particle standard. To analyze the effects of amendments on soil chemical properties, pH and EC were measured. The porosity, capillary porosity, air-filled porosity, bulk density and hydraulic conductivity also measured to analyze the physical properties. Chemical properties were significantly different by mixture ratios of a SFC, compost and cocopeat. Capillary porosity was a factor involved in soil physical properties by blending with a SFC and compost. It was affected on the volume of porosity or hydraulic conductivity. To analyze the correlation of mixture ratio versus to physical characters, the ratios of SFC were significantly different in capillary porosity, air-filled porosity, and hydraulic conductivity. These results indicated that mixing ratios of SFC were affected on soil physicochemical properties such as porosity and hydraulic conductivity of the root zone on the USGA sand green.

• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.44-53
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• 2012

As SCB liquid fertilizer (SCB) produced from or out of livestock manure by slurry composting and biofiltration process was applied in golf course, the effect on soil properties and water quality was little investigated. This study was conducted to evaluate the effect of the SCB liquid fertilizer application on environment by monitoring chemical property of soil and water quality of pond as applied chemical fertilizer (CF) and SCB. SCB application rarely contaminated the soil and pond in golf course and decreased organic matter, CEC and Ca in soil and pH and T-N for water quality of pond. In correlation coefficient between soil property parameters, water quality parameters and water quality items, SCB applied in golf course decreased organic matter and CEC in soil and increased SAR in water quality (P<0.01). Nitrogen applied in golf course with SCB or CF was significantly related to T-N in the soil (P<0.01), but not significantly related to T-N in the pond water. These results showed that SCB application little contaminated soil and pond in golf course, and was expected to control of thatch in soil and algae in pond.

• Asian Journal of Turfgrass Science
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• v.18 no.3
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• pp.141-148
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• 2004

In this report, several factors such as infection time and concentration of bacterial suspension, influencing on transient gene expression in Agrobacterium-mediated transformation were evaluated. An appropriate concentration (O.D 600nm = 1.0-1.2) of bateria and 30 min of infection time showed a higher level of GUS expression. To improve transformation efficiency (TE), friable embryogenic calli (FEC) were bombarded by tungsten particles without plasmid DNA, and then co-cultivated with A. tumefaciens LBA4404 which contains pTOK233 super binary vector, carrying neomycin phosphotransferase (NPTII), hygromycin phosphotransferase (hpt) and$\beta-glucuronidase$ (GUS) genes. Three days after co-cultivation with A. tumefaciens and particle bombardment, FEC cultures were transferred to the selection medium (SM: MS medium supplemented with BA 1mg/l, hygromycin 100mg/l, cefotaxime 250 mg/l and vancomycin 200mg/l). They were cultured for 2 weeks and then transferred to the second SM containing hygromycin 50mg/l, cefotaxime 200 mg/l and vancomycin 100mg/l. Later, stable GUS expression was detected 4 to 6 weeks after transfer to the SM. Further, TE from Agrobacterium-mediated transformation after particle bombardment increased to about 3-folds compared with Agrobacterium-mediated transformation without particle bombardment. In the present study, we established an efficient transformation protocol of zoysiagrass by using A. tumefaciens in the combination with particle bombardment for the first time.

• Asian Journal of Turfgrass Science
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• v.19 no.2
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• pp.85-94
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• 2005

A fungal isolate was newly collected from Zoysiagrass 'Anyang-Joongji' in small circular patches on a fairway ofa golf course in Korea, which seriously occurred during the early summer period of 2005. The isolate presented on PDAmedium, named Scz1, was closely identical to Sclerotinia homoeocarpa, a casual fungus of dollar spot disease, in cool season turf grasses such as creeping bentgrass. Hereby, this study was accomplished to characterize the isolate and compare it with the fungus, named Scb1, isolated from dollar spot-infected creeping bentgrass (Agrostis palustris Huds. cv Penncross). On PDAmedium, individual mycelial appearance of three isolates was very similar except for the pigment. Mycelial pigments of Scz1 and Scz2 (another analogous isolate collected) were light pinkish on the reverse side of PDA medium but that of Scb1 was dark brownish. In a microscopic study, three isolates were barely distinguishable in the appearance of mycelia. As expected, in the temperaturesensitivity assay, all pathogens were very delicate to $32^{circ}C$ above but not to $30^{circ}C$ below, in which was explained to be one of typical characteristics in S. homoeocarpa. In an artificial inoculation assay, disease symptoms including leaf spots in Zoysiagrass were appeared within 6-7 days after inoculation through the hand inoculation method with the isolate-infested soil. Then the fungus was re-identified from the infected leaf tissues. Interestingly, inoculation of isolate Scz1 gave rise to distinct symptoms in only Zoysiagrass but not in creeping bentgrass 'Penncross' and Kentucky bluegrass 'Midnight'. The observation might be involved in host specific pathogenecity of S. homoeocarpa Scz1 to Zoysiagrass. In a chemical sensitivity assay for the isolate, Scz1, showed a high mycelial inhibition against two fungicides, iprodione and propiconazole. All results described above suggest that S. homoeocarpa Scz1 is a primary pathogen of Zoysia dollar spot disease.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.271-278
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• 2009

This study was conducted to investigate the effect of the mixture ratio of the inorganic soil amendments on the soil physicochemical properties. In this experiment, three kinds of soil amendments which had similar pH, EC and particle size, the A, B and C, were tested. The mixture ratio of soil amendment were 0%, 3%, 5%, 7% and 10% (V/V) incorporated with sand which met to the USGA(United State of Golf Association) particle standard. To analyze the effects of amendment on chemical soil properties, pH, EC(electrical conductivity) and CEC(cation exchangeable capacity) were measured. The porosity, bulk density and hydraulic conductivity also measured to analyze the changes of physical properties. In the chemical properties, pH was significantly related to the mixture ratios of amendments, A and C(P<0.05), CEC and EC also related to the ratios of C(P<0.01). When the results were applied to the USGA standard of the soil physical properties, the optimum mixture ratios of each amendment were 3% in A and B, and 7~10% in C. To analyze the corelation of mixture ratio versus to physical character, volume of porosity was significantly related to the ratio of B (P<0.05), and showed similar corelation in porosity and hydraulic conductivity with ratio of C(P<0.05). These results indicate that types and mixture ratio of inorganic soil amendments should affect on soil physio-chemical properties of root zone on USGA sand green.

• Asian Journal of Turfgrass Science
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• v.21 no.2
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• pp.147-154
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• 2007

In March of 2004, gray snow mold (Typhula blight) caused by Typhula spp. occurred on perennial ryegrass (Lolium perenne L.) and Kentucky bluegrass (Poo pratensis L.) at MuJu golf courses in Jeonbuk Province. Leaves in the affected areas were matted together and frequently covered with white to grayish mycelia. Sclerotia were formed on the leaf blade, leaf sheath, or crown regions. The fungus isolated from the diseased leaf formed whitish mycelium, clamp connections, and light pink to brown, irregular-shaped small sclerotia of less than 1.4 mm in diameter, which are characteristic to Typhula incarnata. Optimum temperature ranges for mycelial growth were $5^{\circ}C$ to $15^{\circ}C$. The causal organism was confirmed to be T. incarnata as the partial sequence of its ribosomal RNA ITS1 (internal transcribed spacer) region was 91% homologous to those of T. incarnata in GenBank database. Out of the 14 fungicides tested fur antifungal activity in vitro, 10 fungicides including iprodione, tebuconazole, polyoxin D, flutolanil, hexaconazole, tolclofos-methyl, fosetyl-Al, mepronil, pencycuron+tebuconazole, and fenarimol completely inhibited fungal growth at their recommended concentrations. In the field test, these fungicides and others such as thifluzamide and thiram effectively controlled the gray snow mold of turfgrass with some variable degrees of control efficacies.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.1
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• pp.13-23
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• 2016

This study was conducted to investigate the growth characteristics of warm-season turfgrasses and to find out suitable turfgrass species on Saemangeum reclaimed land. Twenty native zoysiagrass(Zoysia sinica, Z. matrella, Z. japonica, Medium-leaf type zoysiagrass(hybrid zoysiagrass)) and bermudagrass(Cynodon dactylon) collected from Korea were used in this study. Total stolon length and the number of stolon per square meter, relative growth rate of shoot and stolon, and coverage rate were analyzed for 2 year. C. dactylon showed not only the most growth response with high relative growth rate of shoot and stolon, which were 19.9% and 66.3%, but also resulted in higher level of turf visual quality compared to others. Whereas Z. japonica showed the least growth response with low relative growth rate of shoot and stolon, which were 2.4% and 0.7%. Although all warm-season turfgrasses took root and grew up well, there were different growth rates between the interspecies. Z. sinica 'Z2034', Z. matrella 'Z4091', Z. japonica 'Z1064', Medium-leaf type zoysiagrass 'ZN6019' and C. dactylon 'BN7014' were the greatest growth rate of shoot and stolon. These results will be useful for selecting salt tolerant breeding lines and also used to develop a turfgrass cultivar with strong salinity tolerance through continuous monitoring.

• Korean Journal of Weed Science
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• v.30 no.4
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• pp.323-329
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• 2010

Methiozolin (5-(2,6-difluoro-benzyloxymethyl)-5-methyl-3-(3-methyl- thiophen-2-yl)- 4,5-dihydro-isoxazole) is a new turf herbicide in isoxazoline chemistry. The herbicide controls grass weeds and has a high safety to various cool and warm season turfgrasses. This paper describes basic chemical, biological, and regulatory information of methiozolin.

• Weed & Turfgrass Science
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• v.3 no.2
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• pp.114-120
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• 2014

Turfgrass insect pests were investigated in different turf sod production areas of Korea. Twelve insect pest species of 7 families in 6 orders and one Eriophyidae mite, Aceria zoysiae were collected from turf sod production areas. The zoysiagrass mite was most frequently occurred zoysiagrass sod production areas. Damaged rate by zoysiagrass mite was increased from May to September. Noctuidae (Spodoptera depravata, Agrotis ipsilon and A. segetum) and Pyralidae (Crambus sp.) insect pests in Lepidoptera were attracted in A. ipsilon sex pheromone trap. Eggs and larva of A. ipsilon was the highest occurred August. Zoysiagrass mite and A. ipsilon were main insect pests in turf sod production areas in Korea.