• The Korean Journal of Mycology
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• v.50 no.4
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• pp.275-280
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• 2022

Truffles are ectomycorrhizal fungi that belong to the genus Tuber. They exhibit symbiotic relationships, particularly with oak (Quercus spp.) and hazel (Corylus spp.) trees. We performed an inoculation using a spore suspension to synthesize mycorrhizae between European truffles, Tuber borchii and Tuber melanosporum, and an indigenous oak species, Quercus acutissima. This resulted in the formation of mycorrhizae within 2 months after inoculation. Despite having the same host plant, differences in features were observed between Tuber species, including color and mantle type. These results indicate that Q. acutissima is a suitable host plant for truffle cultivation in Korea and provide a better understanding of the mycorrhization of T. borchii and T. melanosporum.

• Mycobiology
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• v.47 no.2
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• pp.180-190
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• 2019

In this study, eight-month-old ectomycorrhizae of Tuber borchii with Corylus avellana were synthesized to explore the influence of T. borchii colonization on the soil properties and the microbial communities associated with C. avellana during the early symbiotic stage. The results showed that the bacterial richness and diversity in the ectomycorrhizae were significantly higher than those in the control roots, whereas the fungal diversity was not changed in response to T. borchii colonization. Tuber was the dominant taxon (82.97%) in ectomycorrhizae. Some pathogenic fungi, including Ilyonectria and Podospora, and other competitive mycorrhizal fungi, such as Hymenochaete, had significantly lower abundance in the T. borchii inoculation treatment. It was found that the ectomycorrhizae of C. avellana contained some more abundant bacterial genera (e.g., Rhizobium, Pedomicrobium, Ilumatobacter, Streptomyces, and Geobacillus) and fungal genera (e.g., Trechispora and Humicola) than the control roots. The properties of rhizosphere soils were also changed by T. borchii colonization, like available nitrogen, available phosphorus and exchangeable magnesium, which indicated a feedback effect of mycorrhizal synthesis on soil properties. Overall, this work highlighted the interactions between the symbionts and the microbes present in the host, which shed light on our understanding of the ecological functions of T. borchii and facilitate its commercial cultivation.