• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제40권1호
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• pp.3-10
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• 2014

Objectives: Although nerve growth factor (NGF) could promote the functional regeneration of an injured peripheral nerve, it is very difficult for NGF to sustain the therapeutic dose in the defect due to its short half-life. In this study, we loaded the NGF-bound heparin-conjugated fibrin (HCF) gel in the NGF-delivering implants and analyzed the time-dependent release of NGF and its bioactivity to evaluate the clinical effectiveness. Materials and Methods: NGF solution was made of 1.0 mg of NGF and 1.0 mL of phosphate buffered saline (PBS). Experimental group A consisted of three implants, in which $0.25{\mu}L$ of NGF solution, $0.75{\mu}L$ of HCF, $1.0{\mu}L$ of fibrinogen and $2.0{\mu}L$ of thrombin was injected via apex hole with micropipette and gelated, were put into the centrifuge tube. Three implants of experimental group B were prepared with the mixture of $0.5{\mu}L$ of NGF solution, $0.5{\mu}L$ HCF, $1.0{\mu}L$ of fibrinogen and $2.0{\mu}L$ of thrombin. These six centrifuge tubes were filled with 1.0 mL of PBS and stirred in the water-filled beaker at 50 rpm. At 1, 3, 5, 7, 10, and 14 days, 1.0 mL of solution in each tubes was collected and preserved at $-20^{\circ}C$ with adding same amount of fresh PBS. Enzyme-linked immunosorbent assay (ELISA) was done to determine in vitro release profile of NGF and its bioactivity was evaluated with neural differentiation of pheochromocytoma (PC12) cells. Results: The average concentration of released NGF in the group A and B increased for the first 5 days and then gradually decreased. Almost all of NGF was released during 10 days. Released NGF from two groups could promote neural differentiation and neurite outgrowth of PC12 cells and these bioactivity was maintained over 14 days. Conclusion: Controlled release system using NGF-HCF gel via NGF-delivering implant could be an another vehicle of delivering NGF to promote the nerve regeneration of dental implant related nerve damage.

• 한국정보전자통신기술학회논문지
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• 제13권5호
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• pp.443-451
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• 2020

하이퍼루프는 튜브 내를 부상한 상태로 초고속주행하는 미래형 이동 시스템으로 제안되었다. 하이퍼루프에서 달리는 포드 차량은 선형 동기모터 상에서 주행하기에 위치측정이 근본적 중요성을 가진다. 1000km/h 이상의 속도로 주행하는 포드 차량의 가속과 감속에 정밀한 위치측정이 요구되며 또한 차량간 거리 유지를 위한 속도 조절에도 요구된다. 본 논문은 laser surface velocimeter를 개량하여 새로운 범위조건의 위치측정을 시도한다. 높은 정밀도로 초고속 이동체의 위치를 측정해야 하기에 지나가는 타일의 레이저 반사율 차이 검출로 변위를 계산하는 상대위치 측정장치를 구현하여 성능시험으로 평가하였다. 500km/h 미만의 포드 속도에서 cm 단위의 정밀도로 정확한 위치 측정을 보였으며, 500km/h 이상의 포드 속도에서도 0.1% 미만의 매우 낮은 오차율로 위치측정 결과를 보여주었다. 향후 500km/h 이상에서의 오차율도 0에 수렴하도록 연구를 진행해야 한다.