• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1997년도 춘계학술발표회논문집(1)
• /
• pp.587-591
• /
• 1997

The DGs (Diesel Generators) in NPP (Nuclear Power Plant) has been used for the emergency electric power source to shut down the nuclear reactor safely in case of station blackout. The RCM (Reliability Centered Maintenance) has been applied to DGs for increasing the safety of NPP. The structured defects of DG were not remedied by the improvement of maintenance method. As the first stage of RCM, to find the structured defects, its failure modes were searched and analyzed through the ten year maintenance information. The structured defects such as the air compressor, the lubricating oil pressure, and the insufficient load were the root causes of main failures. The air reservoir reinstallation, the lubricating oil tube modification, the load bank installation, and the qualitative instrumentation were the solutions for the hardware oriented RCM of DGs. There remains the software oriented RCM such as the rejection of useless maintenance, the preventive maintenance, the database of maintenance information, and the predictive maintenance.

• 한국태양에너지학회:학술대회논문집
• /
• 한국태양에너지학회 2011년도 춘계학술발표대회 논문집
• /
• pp.86-91
• /
• 2011

Thermal design was conducted for a solar thermal storage system in a medium-temperature range between $200^{\circ}C$ and $400^{\circ}C$. The system was composed of heat pipes as heat carrier and molten salts as phase-change storage material. Each heat pipe penetrated through the storage system and had two heat-exchanging sections at both ends to interact with high-and low-temperature steams, while it exchanged heat with molten salts in the middle section. During a heat-storage mode, the heat pipes transferred heat from the hot steam at one side to the molten salts and it transferred heat from the molten salt to the cold steam at the other side during the heat-dissipating mode. A tube-bank type heat exchanger theory was applied to this design task to meet the required inlet and outlet temperatures of the steams depending on the operation modes. Several design variables were considered including the lengths of evaporator and condenser of a heat pipe, traverse and longitudinal pitches of the pipe, and the number of rows of the heat pipes for two different molten salt baths. An optimum design results were presented with discussion.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권9호
• /
• pp.1229-1233
• /
• 2006

To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.

• Journal of Microbiology
• /
• 제40권3호
• /
• pp.199-204
• /
• 2002

A microsporidium, from cabbage white bntteflies, Pieris rapae, collected in Korea, was purified and characterized according to its gene structure, spore morphology and pathogenicity. From the observation of the isolate by SEM and TEM, the endospores, exospores and nuclei, about 12 polar filament coils of the polar tube and posterior vacuoles were all identified. The nucleotide sequence was determined for a portion of genomic DNA which spans the V4 variable region of the small subunit rRNA gene. Comparison with the GenBank database for 15 other microsporidia species suggests that this isolate is most closely related to Nosema species. The pathogenicity against cabbage white butterflies was quantified by inoculating variable doses of spores to the second instar larvae. Peroral inoculation at a dosage of 10$\^$8/ spores/ml resulted in the death of all larvae prior to adult eclosion, but at lower spore dosages of 10$\^$4/-10$\^$5/ spores/ml, many adults successfully emerged. The median lethal dose (LD$\_$50/) was deter-mined to be 4.6$\times$10$\^$6/ spores/ml and the isolate also transmitted transovarially to the progeny eggs at a frequency of 92%.

• 대한수혈학회지
• /
• 제23권2호
• /
• pp.127-135
• /
• 2012

배경: 대부분의 임상검사가 자동화되는 추세이나 혈액은행 검사는 여전히 수작업에 의존하고 있다. 여러 자동화장비가 개발되어 활용되고 있으며 최근 면역혈액검사를 위한 자동화장비인 IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA)이 소개되었다. 이번 연구에서는 IH-1000 장비의 ABO 및 Rh 혈액형 검사와 비예기항체 선별검사에 대한 성능을 평가하려고 한다. 방법: 2011년 10월에 의뢰된 373건의 ABO 및 RhD 혈액형 검사와 303건의 비예기항체 선별검사를 대상으로 하였다. ABO 및 RhD 혈액형 검사는 슬라이드법과 시험관법을 사용하였고 비예기항체검사는 미세원주응집법(DiaMed-ID system)을 사용하여 IH-1000의 결과와 비교하였다. 결과: ABO 및 RhD 혈액형 검사에서는 두 방법 간 100%의 높은 일치율을 보였고 비예기항체 선별검사에서는 두 방법에서 모두 양성을 보인 검체가 10건(anti-E+c 4건, anti-E 3건, anti-D 1건, anti-M 1건, anti-Xg 1건)이었고 모두 음성을 보인 검체는 289건으로 98.7%의 일치율을 보였다. IH-1000의 민감도 및 특이도는 각각 90.9%였다. 결론: IH-1000은 기존에 사용하던 수기법과 비교하여 ABO 및 Rh 혈액형 검사와 비예기항체 선별검사에서 높은 일치도를 보였다. 따라서 혈액은행에서의 수혈 전 일상검사에 자동화 장비인 IH-1000을 사용할 수 있을 것으로 생각된다.