• 디지털융복합연구
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• 제10권3호
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• pp.127-135
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• 2012

본 논문에서는 기존의 ID, PW를 이용한 사용자 식별 인증 시스템 방식을 보완하여 ID, PW, OTP를 이용한 사용자 인증과 네트워크상의 컴퓨터 구별 방법 중 MAC Address를 이용한 방법을 이용하여 비인가 사용자 접근을 거부하는 시스템에 대한 프로토콜을 설계하고 구현한다. 비인가 시용자의 인증 시도에 대한 안전성 분석에서는 비인가 사용자의 정보 취득 정도를 네 가지로 나누어 안전성에 대해 분석했다.

• 디지털융복합연구
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• 제10권2호
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• pp.183-191
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• 2012

본 논문에서는 사용자를 인증하는 방식 중 자신이 알고 있는 것과 소유하고 있는 것, 두 가지를 이용한 인증 모델을 모바일 OTP 구현검토 사항에 만족하는 모바일 OTP 생성 알고리즘과 안전한 OTP 추출 알고리즘으로 구성된 모바일 OTP 생성 모델을 제안한다. 기존 OTP 기반 시스템의 보안성을 높이기 위해서 모바일 OTP 생성단계에서 수행되는 사용자의 개인정보인 회원번호, 랜덤번호를 사용하여 보안의 안전성을 향상시켰다.

• 응용통계연구
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• 제32권5호
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• pp.693-702
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• 2019

딥러닝은 대용량의 데이터의 분류 및 예측하는 방법으로 각광받고 있다. 데이터의 양이 많아지면서 신경망의 구조는 더 깊어 지고 있다. 이때 초기값이 지나치게 클 경우 층이 깊어 질수록 활성화 함수의 기울기가 매우 작아지는 포화(Saturation)현상이 발생한다. 이러한 포화현상은 가중치의 학습능력을 저하시키는 현상을 발생시키기 때문에 초기값의 중요성이 커지고 있다.이런 포화현상 문제를 해결하기 위해 Glorot과 Bengio (2010)과 He 등 (2015) 층과 층 사이에 데이터가 다양하게 흘러야 효율적인 신경망학습이 가능하고 주장했다. 데이터가 다양하게 흐르기 위해서는 각 층의 출력에 대한 분산과 입력에 대한 분산이 동일해야 한다고 제안했다. Glorot과 Bengio (2010)과 He 등 (2015)는 각 층별 활성화 값의 분산이 같다고 가정해 초기값을 설정하였다. 본 논문에서는 절단된 코쉬 분포와 절단된 정규분포를 활용하여 초기값을 설정하는 방안을 제안한다. 출력에 대한 분산과 입력에 대한 분산의 값을 동일하게 맞춰주고 그 값이 절단된 확률분포의 분산과 같게 적용함으로써 큰 초기값이 나오는 걸 제한하고 0에 가까운 값이 나오도록 분포를 조정하였다. 제안된 방법은 MNIST 데이터와 CIFAR-10 데이터를 DNN과 CNN 모델에 각각 적용하여 실험함으로써 기존의 초기값 설정방법보다 모델의 성능을 좋게 한다는 것을 보였다.

• Animal Bioscience
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• 제34권5호
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• pp.867-879
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• 2021

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

• Fisheries and Aquatic Sciences
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• 제25권5호
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• pp.287-297
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• 2022

The objective of this study was to evaluate the probability of norovirus foodborne illness by raw oyster consumption. One hundred fifty-six oyster samples were collected to examine the norovirus prevalence. The oyster samples were inoculated with murine norovirus and stored at 4℃-25℃. A plaque assay determined norovirus titers. The norovirus titers were fitted with the Baranyi model to calculate shoulder period (h) and death rate (Log PFU/g/h). These kinetic parameters were fitted to a polynomial model as a function of temperature. Distribution temperature and time were surveyed, and consumption data were surveyed. A dose-response model was also searched through literature. The simulation model was prepared with these data in @RISK to estimate the probability of norovirus foodborne. One sample of 156 samples was norovirus positive. Thus, the initial contamination level was estimated by the Beta distribution (2, 156), and the level was -5.3 Log PFU/g. The developed predictive models showed that the norovirus titers decreased in oysters under the storage conditions simulated with the Uniform distribution (0.325, 1.643) for time and the Pert distribution (10, 18, 25) for temperature. Consumption ratio of raw oyster was 0.98%, and average consumption amount was 1.82 g, calculated by the Pert distribution [Pert {1.8200, 1.8200, 335.30, Truncate (0, 236.8)}]. ₁F₁ hypergeometric dose-response model [1 - (1 + 2.55 × 10^-3 × dose)^-0.086] was appropriate to evaluate dose-response. The simulation showed that the probability of norovirus foodborne illness by raw oyster consumption was 5.90 × 10^-10 per person per day. The annual socioeconomic cost of consuming raw oysters contaminated with norovirus was not very high.