• Journal of Digital Convergence
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• v.10 no.3
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• pp.127-135
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• 2012

This study improves the existing user identification method using user ID and password. The new protocol protecting unauthorized access is designed and developed using user's ID, password, and OTP for user certification as well as MAC address for computer identification on networks. The safety on trials of unauthorized certification is tested and analyzed for four types following the criterion of information acquisition.

• Journal of Digital Convergence
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• v.10 no.2
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• pp.183-191
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• 2012

This study proposes dual certification model using both what users know and what users own. In detail, this mobile OTP generation model is made up of mobile OTP generation and extraction algorithm satisfying the conditions for reviewing mobile OTP implementation. In order to improve the security of the existing OTP-based systems, the suggested method utilizes user's ID and random number at the mobile OTP generation stage.

• The Korean Journal of Applied Statistics
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• v.32 no.5
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• pp.693-702
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• 2019

Deep learning has gained popularity for the classification and prediction task. Neural network layers become deeper as more data becomes available. Saturation is the phenomenon that the gradient of an activation function gets closer to 0 and can happen when the value of weight is too big. Increased importance has been placed on the issue of saturation which limits the ability of weight to learn. To resolve this problem, Glorot and Bengio (Proceedings of the Thirteenth International Conference on Artificial Intelligence and Statistics, 249-256, 2010) claimed that efficient neural network training is possible when data flows variously between layers. They argued that variance over the output of each layer and variance over input of each layer are equal. They proposed a method of initialization that the variance of the output of each layer and the variance of the input should be the same. In this paper, we propose a new method of establishing initialization by adopting truncated normal distribution and truncated cauchy distribution. We decide where to truncate the distribution while adapting the initialization method by Glorot and Bengio (2010). Variances are made over output and input equal that are then accomplished by setting variances equal to the variance of truncated distribution. It manipulates the distribution so that the initial values of weights would not grow so large and with values that simultaneously get close to zero. To compare the performance of our proposed method with existing methods, we conducted experiments on MNIST and CIFAR-10 data using DNN and CNN. Our proposed method outperformed existing methods in terms of accuracy.

• Animal Bioscience
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• v.34 no.5
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• pp.867-879
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• 2021

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

• Fisheries and Aquatic Sciences
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• v.25 no.5
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• pp.287-297
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• 2022

The objective of this study was to evaluate the probability of norovirus foodborne illness by raw oyster consumption. One hundred fifty-six oyster samples were collected to examine the norovirus prevalence. The oyster samples were inoculated with murine norovirus and stored at 4℃-25℃. A plaque assay determined norovirus titers. The norovirus titers were fitted with the Baranyi model to calculate shoulder period (h) and death rate (Log PFU/g/h). These kinetic parameters were fitted to a polynomial model as a function of temperature. Distribution temperature and time were surveyed, and consumption data were surveyed. A dose-response model was also searched through literature. The simulation model was prepared with these data in @RISK to estimate the probability of norovirus foodborne. One sample of 156 samples was norovirus positive. Thus, the initial contamination level was estimated by the Beta distribution (2, 156), and the level was -5.3 Log PFU/g. The developed predictive models showed that the norovirus titers decreased in oysters under the storage conditions simulated with the Uniform distribution (0.325, 1.643) for time and the Pert distribution (10, 18, 25) for temperature. Consumption ratio of raw oyster was 0.98%, and average consumption amount was 1.82 g, calculated by the Pert distribution [Pert {1.8200, 1.8200, 335.30, Truncate (0, 236.8)}]. ₁F₁ hypergeometric dose-response model [1 - (1 + 2.55 × 10^-3 × dose)^-0.086] was appropriate to evaluate dose-response. The simulation showed that the probability of norovirus foodborne illness by raw oyster consumption was 5.90 × 10^-10 per person per day. The annual socioeconomic cost of consuming raw oysters contaminated with norovirus was not very high.