• Korean Journal of Microbiology
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• v.22 no.4
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• pp.207-214
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• 1984

Improved methods for the isolation and purification of conidial protoplast were investigated and conidial proplast from auxotrophic mutants were fused. The reaction time for isolation of protoplasts from the swollen condiospores preincubated in liquid minimal medium supplimented with 2-deoxy-D-glucose was shorten by reaction with mixture of 2% driselase and 2% ${\beta}-glucuronidase$ (1:1). The conidial protoplast could be highly purified by using 5% Ficoll 400 as a centrifugation medium. Nucleus of the conidial protoplast was stained with Giemsa stain and the conidial protoplast had one nucleus. It was also confirmed that the conidial protoplast was true protoplast with no cell wall remnant at the outside of plasma menbrane. Fusion frequencies of the conidial protoplast from auxotrophic mutants ranged from $3.4{\times}10^{-1}\;to\;4.9{\times}10^{-1}$. These values were higher than those of mycelial protoplast by a factor of 5 to 28.

• Journal of Korea Multimedia Society
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• v.12 no.8
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• pp.1128-1141
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• 2009

Graphical expressions are continuously improving, spurred by the astonishing growth of the game technology industry. Despite such improvements, users are still demanding a more natural gaming environment and true reflections of human emotions. In real life, people can read a person's moods from facial color and expression. Hence, interactive facial colors in game characters provide a deeper level of reality. In this paper we propose a facial color adaptive technique, which is a combination of an emotional model based on human emotion theory, emotional expression pattern using colors of animation contents, and emotional reaction speed function based on human personality theory, as opposed to past methods that expressed emotion through blood flow, pulse, or skin temperature. Experiments show this of expression of the Facial Color Model based on facial color adoptive technique and expression of the animation contents is effective in conveying character emotions. Moreover, the proposed Facial Color Adaptive Technique can be applied not only to 2D games, but to 3D games as well.

• Korean Journal of Medicinal Crop Science
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• v.1 no.1
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• pp.16-23
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• 1993

Seeds of Acanthopanx plants need about 2 years to germinate after sowing even if full matured fruits were harvested. I leave a room for doubt that this phenomenon could be brought by the immatured embryo in seed. If it is true, stratification method for the seed propagation of Acanthopanax plants would be used more effectively to promote the growth of embryo in relatively short time. Before stratification, seeds were devided into two parts. One of them was treated for 24hrs in the concentration of $GA_3$ 100 ppm. After stratification of non-treated and treated seeds, seeds for microtechnique were taken on interval of one month for three months, and fixed in Farmer's solution. The seed sizes of Acanthopanax plants were in biggest order A. sessiliflorus, A. seoulense, A. chiisanensis, A. koreanum and A. sieboldianum. The dehiscence phenomenon of seed coat didn't show in most of the seeds that stratified, but A. koreanum only dehisced in seeds treated in $GA_3$ 100ppm. The embryos of the stratified seeds that treated in $GA_3$ showed nomal growth, complete cotyledons and procambium in hypocotyl in any species, but the non-treated seeds could not expect the germination after sowing due to poor growing of the embryo in all species. In view of the results so far achieved, it has taken long times from sowing to germinate because the seed of Acanthopanax plants has immatured embryo.

• Water Engineering Research
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• v.2 no.2
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• pp.139-148
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• 2001

The teleconnections between El Nino/Southern Oscillation (ENSO) and droughts in Korea and the continental United States(U.S.) are investigated using cross analysis. For this purpose, monthly ENSO data and Palmer Drought Severity Index (PDSI) for Korea and for seven states in the U.S. are used. This study shows that there are significant statistical associations between ENSO indices and PDSI for Korea; however, the associations are very weak. It is found that dry conditions in Korea are positively correlated with El Nino, while wet conditions with La Nina. SOI, SSt in the Nino 4 and Ship track 6 regions among ENSO indices are more strongly correlated with PDSI than the other ENSO indices when using the original standardized data, but the SST Nino 3, SST Nino 4, and Darwin SSP exhibit abetter correlations with PDSI when using filtered data to be removed autocorrelation components of the original standardized data. The response time lag for maximum correlation between ENSO indices and PDSI appears to be affected by filtering the data. This is expecially true for Korea than for state analyzed in U.S. In addition, it is found that the PDSI in the continental U.S. is more strongly correlated wiht ENSO than in Korea. Furthermore, in analyzing the El Nino and La Nina aggregate composite data, it is found that the dry anomalies in Korea occur from the year following El Nino to about tow years after while the wet anomalies occur from La Nina year for a period of about two years.

• Proceedings of the KIPE Conference
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• 2007.11a
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• pp.133-135
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• 2007

This research presented the new zero-current switching pulse width modulation SEPP(Single Ended Push-Pull)high frequency inverter for solving the problem of the zero-current SEPP high frequency inverter circuit which is using widely in the practical application of an induction heating apparatus, the soft switching operation and power control are impossible when the lowest power supply in the zero-current switching pulse width modulation SEPP high frequency inverter. The inverter circuit which is attempted by on-off operation of a switch has the reduction effect of the power loss due to a soft switching and a high frequency switching. And it confirmed that the power regulation is possible continuously from 0.25[kW] until 2.84[kW] in the case the duty rate(D) changes from 0.08 to 0.3 under zero-current switching operating by a dissymmetry pulse width modulating control and the power conversion efficiency comes true the efficiency of 95[%]. Due to the result above, the ZCS PWM SEPP high frequency inverter will be effective as sources of an induction heating apparatus.

• Proceedings of the KIEE Conference
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• 2007.11c
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• pp.119-124
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• 2007

This research presented the new zero-current switching pulse width modulation SEPP(Single Ended Push-Pull)high frequency inverter for solving the problem of the zero-current SEPP high frequency inverter circuit which is using widely in the practical application of an induction heating apparatus, the soft switching operation and power control are impossible when the lowest power supply in the zero-current switching pulse width modulation SEPP high frequency inverter. The inverter circuit which is attempted by on-off operation of a switch has the reduction effect of the power loss due to a soft switching and a high frequency switching. And it confirmed that the power regulation is possible continuously from 0.25[kW] until 2.84[kW] in the case the duty rate(D) changes from 0.08 to 0.3 under zero-current switching operating by a dissymmetry pulse width modulating control and the power conversion efficiency comes true the efficiency of 95[%]. Due to the result above, the ZCS PWM SEPP high frequency inverter will be effective as sources of an induction heating apparatus.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.170-177
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• 2003

This paper proposes a robust method for the Ramberg-Osgood (R-O) fit to accurately estimate elastic-plastic J from engineering fracture mechanics analysis based on deformation plasticity. The proposal is based on engineering stress-strain data to determine the R-O parameters, instead of true stress-strain data. Moreover, for practical applications, the method is given not only for the case when full stress-strain data are available but also for the case when only yield and tensile strengths are available. Reliability of the proposed method for the R-O fit is validated against detailed 3-D Finite Element (FE) analyses for circumferential through-wall cracked pipes under global bending using five different materials, three stainless steels and two ferritic steels. Taking the FE J results based on incremental plasticity using actual stress-strain data as reference, the FE J results based on deformation plasticity using various R-O fits are compared with reference J values. Comparisons show that the proposed R-O fit provides more accurate J values for all cases, compared to existing methods for the R-O fit. Advantages of the proposed R-O fit in practical applications are discussed, together with its accuracy.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.30 no.2 s.245
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• pp.194-201
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• 2006

Gait analysis is essential to identify accurate cause and knee condition from patients who display abnormal walking. Traditional linear tools can, however, mask the true structure of motor variability, since biomechanical data from a few strides during the gait have limitation to understanding the system. Therefore, it is necessary to propose a more precise dynamic method. The chaos analysis, a nonlinear technique, focuses on understand how variations in the gait pattern change over time. Eight healthy eight subjects walked on a treadmill for 100 seconds at 60 Hz. Three dimensional walking kinematic data were obtained using two cameras and KWON3D motion analyzer. The largest Lyapunov exponent from the measured knee angular displacement time series was calculated to quantify local stability. This study quantified the variability present in time series generated from gait parameter via chaos analysis. Knee flexion-extension patterns were found to be chaotic. The proposed Lyapunov exponent can be used in rehabilitation and diagnosis of recoverable patients.

• Korean Journal Plant Pathology
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• v.1 no.3
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• pp.173-177
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• 1985

The objective of the study is to determine differences between cucurbits in the pathogenicity of Didymella bryoniae isolated from the naturally infected seeds of cucumber and pumpkin. Primary seedling infection of cucumber(Cucumis sativus L.), oriental melon(Cucumis melo var. makuwa Makino), pumpkin(Cucurbita pepo L.) and watermelon (Citrullus vulgaris Shrad.) occurred on the radicle, hypocotyl and cotyledons and symptoms on each crop were very similar. Infection of the radicle generally caused pre-emergence rot, while infection on the hypocotyl and cotyledons provided further inoculum for infection of the first true leaves and the stem. In cross inoculation tests, all isolates of D. bryoniae could infect cucumber, oriental melon, pumpkin and watermelon at different growth stages and there were not much differences in pathogenicity or susceptibility between isolates of the pathogen and crops tested. The susceptibility of cucumber and pumpkin was markedly influenced by prevailing humid conditions.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2008.10a
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• pp.241-243
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• 2008

This research presented the new zero-current switching pulse width modulation SEPP(Single Ended Push-Full)high frequency inverter for solving the problem of the zero-current SEPP high frequency inverter circuit which is using widely in the practical application of an induction heating apparatus, the soft switching operation and power control are impossible when the lowest power supply in the zero-current switching pulse width modulation SEPP high frequency inverter. The inverter circuit which is attempted by on-off operation of a switch has the reduction effect of the power loss due to a soft switching and a high frequency switching. And it confirmed that the power regulation is possible continuously from 0.25[kW] until 2.84[kW] in the case the duty rate(D) changes from 0.08 to 0.3 under zero-current switching operating by a dissymmetry pulse width modulating control and the power conversion efficiency comes true the efficiency of 95[%]. Due to the result above, the ZCS PWM SEPP high frequency inverter will be effective as sources of an induction heating apparatus.