• Asian-Australasian Journal of Animal Sciences
• /
• v.6 no.4
• /
• pp.607-617
• /
• 1993

Three experiments were carried out in which lactating Hereford ${\times}$ Friesian cattle walked up to ten kilometers a day for three periods of five days with two non-walking days between each walking period and in which the animals were fed different diets. Measurements were made of milk yield, milk constituent yields and concentrations and blood metabolite concentrations. Exercise caused significant reductions in milk yield and in the yields of lactose and milk protein. Milk fat yield was not reduced when animals were exercised. During exercise the concentrations of ${\beta}-OH$ butyrate and free fatty acids increased, whereas the concentrations of glucose, magnesium and inorganic phosphorus decreased. Diet influenced the effect of exercise on some blood metabolite concentrations.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.6
• /
• pp.951-955
• /
• 1999

The effects of restricting feed [60% of ad libitum (AL) intake] (FR) or water provided from 0900 h to 1600 h, daily) (WR) or both (FWR) from 14 to 42 days of age on heterophil/lymphocyte (H/L) response and performance in broiler chickens under the hot humid tropical conditions were determined. Feed and/or water limitation retarded growth, but had no adverse effect on overall feed conversion ratio and survivability. The trend for total feed and water consumption was similar to body weight pattern with AL>WR>FR>FWR. The nutritional regimens had significant effect on overall water:feed ratios with FR>(AL=WR)>FWR. Restriction of feed and/or water resulted in marked elevation of H/L ratios. As measured by H/L ratios, the effect of adapting to FR dissipated between 16 to 21 days after the onset of feed restriction. The H/L ratios of WR and FWR birds remained elevated throughout the duration of the experiment.

• Parasites, Hosts and Diseases
• /
• v.62 no.1
• /
• pp.53-63
• /
• 2024

The intracellular parasite Babesia microti is among the most significant species causing human babesiosis and is an emerging threat to human health worldwide. Unravelling the pathogenic molecular mechanisms of babesiosis is crucial in developing new diagnostic and preventive methods. This study assessed how priming with B. microti surface antigen 1 (BHSA 1) and seroreactive antigen 5-1-1 (BHSA 5-1-1) mediate protection against B. microti infection. The results showed that 500 ㎍/ml rBMSA1 and rBMSA5-1-1 partially inhibited the invasion of B. microti in vitro by 42.0±3.0%, and 48.0±2.1%, respectively. Blood smears revealed that peak infection at 7 days post-infection (dpi) was 19.6%, 24.7%, and 46.7% in the rBMSA1, rBmSA5-1-1, compared to the control groups (healthy mice infected with B. microti only), respectively. Routine blood tests showed higher white blood cell, red blood cell counts, and haemoglobin levels in the 2 groups (BMSA1 and BMSA5 5-1-1) than in the infection control group at 0-28 dpi. Moreover, the 2 groups had higher serum interferon-γ, tumor necrosis factor-α and Interleukin-17A levels, and lower IL-10 levels than the infection control group throughout the study. These 2 potential vaccine candidate proteins partially inhibit in vitro and in vivo B. microti infection and enhance host immunological response against B. microti infection.

• Parasites, Hosts and Diseases
• /
• v.51 no.5
• /
• pp.589-594
• /
• 2013

In intestinal helminth infections, Th2 immune respones are generally associated with mucin secretion for worm expulsion from the host intestine. In particular, IL-4 and IL-13 are the important cytokines related with intestinal mucus production via STAT6 signalling in nematode infections. However, this perspective has never been studied in Gymnophalloides seoi infection. The present study aimed to observe the STAT6 signalling and cytokine responses in C57BL/6 mice, a mouse strain resistant to infection with this trematode. The results showed that worm expulsion occurred actively during days 1-2 post-infection (PI), when goblet cells began to proliferate in the small intestine. The STAT6 gene expression in the mouse spleen became remarkable from day 2 PI. Moreover, G. seoi infection induced a significant increase of IL-13 from day 4 PI in the spleen of infected mice. Our results suggested that goblet cell hyperplasia and worm expulsion in G. seoi-infected mice should be induced by STAT6 signalling, in which IL-13 may be involved as a dominant triggering cytokine.

• Atmosphere
• /
• v.23 no.1
• /
• pp.1-11
• /
• 2013

The angular momentum transport of an idealized axisymmetric vortex in the developing stage was investigated using the Weather Research and Forecast (WRF) model. The balanced axisymmetric vortex was constructed based on an empirical function for tangential wind, and the temperature, geopotential, and surface pressure were obtained from the balanced equation. The numerical simulation was carried out for 6 days on the f-plane with the Sea Surface Temperature (SST) set as constant. The weak vortex at initial time was intensified with time, and reached the strength of tropical cyclone in a couple of days. The Absolute Angular Momentum (AAM) was transported along with the secondary circulation of the vortex. Total AAM integrated over a cylinder of radius of 2000 km decreased with simulation time, but total kinetic energy increased rapidly. From the budget analysis, it was found that the surface friction is mainly responsible for the decrease of total AAM. Also, contribution of the surface friction to the AAM loss was about 90% while that of horizontal advection was as small as 8%. The trajectory of neutral numerical tracers following the secondary circulation was presented for the Lagrangian viewpoint of the transports of absolute angular momentum. From the analysis using the trajectory of tracers it was found that the air parcel was under the influence of the surface friction continuously until it leaves the boundary layer near the core. Then the air parcel with reduced amount of angular momentum compared to its original amount was transported from boundary layer to upper level of the vortex and contributed to form the anti-cyclone. These results suggest that the tropical cyclone loses angular momentum as it develops, which is due to the dissipation of angular momentum by the surface friction.

• Journal of the Korean earth science society
• /
• v.30 no.5
• /
• pp.529-549
• /
• 2009

In 2007, just after the recession of the Changma, anomalously long rainy period (from July 30 to August 15) occurred in Korea. To identify the cause of the sustained rainy period, we performed synoptic analysis and the associated air motions. The behavior of each air parcel trajectory associated with atmospheric motion was then investigated. As a result, three particular phenomena occurring at latitudes lower than $40^{\circ}N$ were discovered. First, a mass of relatively cold air, referred to as E, made a deep intrusion from $20^{\circ}N$ to $60^{\circ}N$. Second, this intrusion was accompanied by another mass of air called dE. It was colder and drier than E and originated from the mid-troposphere over the tropical ocean. Third, dE and E rotated clockwise three times over a period of 17 days over the Northwestern Pacific and blocked the westerly waves imbedded in the zonal flow from propagating. Two additional phenomena were observed at latitudes higher than $40^{\circ}N$. First, the cold core system, while approaching from the west with low geopotential values at its center, was stagnated over Shanxi China. It enhanced the northward intrusion of dE and E, and then diminished. The subsequent low system showed similar evolution as the first one. Second, a warm core anticyclone was formed over Lake Baikal, blocking the westerlies for 13 days and contributed to the persistent northward incursion of warm moist air. Moreover, a horizontally extended intrusion of upper level clouds from the tropics to $50^{\circ}N$, which may be interpreted as a tropical plume, was found around the end of the period (from August 12 to 15, 2007) with successive tropical nights over Korea.

• Parasites, Hosts and Diseases
• /
• v.25 no.2
• /
• pp.195-198
• /
• 1987

In order to test the function of Iymphocytes in Naegleria fowleri-nniected mice, the in nitro blastogenic response of splenocyte cultures to non-specific mitogens was studied. Concanavalin A and lipopolysaccharide stimulation were used as tests of T cell and B cell function. For the first 14 days following N. fowleri infection, Iymphoblastic transformation induced by T-cell mitogen was markedly reduced in comparison to the uninfected control mice. The blastogenic response to B-cell mitogen remained depressed in the infected mice up to 14 days after infection. The fluorescent antibody titers of sera of N. fowleri infected mice were between 1 : 4 and 1 : 32. The results suggest that there is a suppression of cell mediated immunity during the acute course of experimental Naegleria meningoencephalitis in mice.

• Parasites, Hosts and Diseases
• /
• v.57 no.3
• /
• pp.225-232
• /
• 2019

Innate lymphoid cells (ILCs) are key players during an immune response at the mucosal surfaces, such as lung, skin, and gastrointestinal tract. Giardia lamblia is an extracellular protozoan pathogen that inhabits the human small intestine. In this study, ILCs prepared from the lamina propria of mouse small intestine were incubated with G. lamblia trophozoites. Transcriptional changes in G. lamblia-exposed ILCs resulted in identification of activation of several immune pathways. Secretion of interleukin (IL)-17A, IL-17F, $IL-1{\beta}$, and interferon-${\gamma}$ was increased, whereas levels of IL-13, IL-5, and IL-22, was maintained or reduced upon exposure to G. lamblia. Goup 3 ILC (ILC3) was found to be dominant amongst the ILCs, and increased significantly upon co-cultivation with G. lamblia trophozoites. Oral inoculation of G. lamblia trophozoites into mice resulted in their presence in the small intestine, of which, the highest number of parasites was detected at the 5 days-post infection. Increased ILC3 was observed amongst the ILC population at the 5 days-post infection. These findings indicate that ILC3 from the lamina propria secretes IL-17 in response to G. lamblia, leading to the intestinal pathology observed in giardiasis.

• Environmental Engineering Research
• /
• v.19 no.3
• /
• pp.261-268
• /
• 2014

Leachate quality and methane emission from pilot-scale lysimeters operated under semi-aerobic and anaerobic conditions were monitored for 650 days. Two semi-aerobic lysimeters were filled with un-compacted and compacted municipal solid wastes whereas two anaerobic lysimeters containing compacted wastes were operated with leachate storage at 50% and 100% of waste height, respectively. Despite having high moisture in wastes and operating under tropical rainfall events, leachate stabilization in semi-aerobic lysimeters took place much faster resulting in BOD reduction by 90% within 60 days, significantly shorter than 180-210 days observed in anaerobic lysimeters. Nitrogen concentration in leachate from semi-aerobic lysimeter could be reduced by 90%. In term of gas emission, semi-aerobic lysimeter with un-compacted wastes had much lower methane emission rate of $2.8g/m^2/day$ compare to anaerobic lysimeters ($62.6g/m^2/day$) through seasonal fluctuation was observed. Nevertheless, semi-aerobic lysimeter with waste compaction has similar performance to anaerobic lysimeter.

• Parasites, Hosts and Diseases
• /
• v.30 no.2
• /
• pp.151-153
• /
• 1992

We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall $0.7^{\circ}C$/min) than by fast cooling (overall $1.3^{\circ}C$/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophosoites. The survival rate was 2∼39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.