• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.571-578
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• 1997

The development of new approaches for wheat grain hardness assessment may impact the grain industry in marketing, milling and breeding. This experiment was to develop a new method for fast identification between softness and hardness, and for maintaining germinability of seed after measurement in wheat. Results from the comparisons of accuracy and significance between image analysis and conventional methods(NIRS and textrometer) were summarized. Data obatined from image analysis for grain hardness did not show any difference from those of the conventional methods. The protein content analyzed by micro-Kjeldahl method was significantly correlated with the grain hardness measured by image analysis, textrometer, and NIRS. The analysis for wheat grain hardness using image analysis may be used as an alternative method to the conventional methods. This method also takes the seeds after analysis can be utilized as breeding materials in early generations.

• Plant Resources
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• v.6 no.2
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• pp.153-158
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• 2003

Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.

• Mycobiology
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• v.39 no.2
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• pp.113-117
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• 2011

A laboratory experiment was conducted to study the induction of constricting rings and test predation of Dactylaria brochopaga isolates against second stage juveniles (J2s) of Meloidogyne graminicola. Among the five fungal isolates, isolate D showed the greatest number of predatory rings and, consequently, trapped the maximum number of M. graminicola J2s in dual cultures. Another pot experiment was conducted to study the effect of D. brochopaga (isolate D) on the management of wheat root-knot disease. Applying a mass culture (10 g/pot) and a spore suspension of the fungus with and without cow dung manure to soil infested with 2,000 M. graminicola juveniles significantly improved plant height, root length, weights of shoots, roots, panicles and grains per hill compared to those in the control. Moreover, the fungus significantly reduced the number of root-knots, the number of egg masses, juveniles, and females per hill compared to those in the control. Bio-efficacy of the fungus was heightened when the mass culture and a spore suspensions were used in combination with cow dung manure to improve the plant growth parameters and reduce the number of root-knot and reproductive factors. Further investigations should be conducted to identify the impact of this fungus in the field.

• Plant Breeding and Biotechnology
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• v.6 no.4
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• pp.363-380
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• 2018

Wheat is frequently exposed to high temperature during anthesis and ripening period, which resulted in yield loss and detrimental end-use-quality. The transcriptome analysis of wheat under high temperature stress during the early stage of the grain filling period was undertaken. Three expression patterns of differentially expressed genes (DEGs) during grain filling period were identified. The DEGs of seed storage protein and starch-branching enzyme showed continuous increases in their expressions during high temperature stress, as well as during the recovery period. The activities of the enzymes responsible for the elimination of antioxidants were significantly affected by exposure to high temperature stress. Only the WAS-2 family genes showed increased transcription levels under high temperature stress in dehulled spikelets. The relative transcription levels for sub-genome specific WAS-2 genes suggested that WAS-2 genes reacted with over-expression under high temperature stress and decreased back to normal expression during recovery. We propose the role of WAS-2 as a protective mechanism during the stage of grain development under high temperature in spikelets.

• Korean Journal of Environmental Agriculture
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• v.19 no.5
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• pp.375-381
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• 2000

Quantifying the changes of soil microbial biomass and activity of enzymes are important to understand the dynamics of active soil C and N pools. The dynamics of soil microbial biomass C and N and the activity of enzymes over entire growth period of soybean-(Glycine max (L) Merr.)-wheat (Triticum aestivum L.) sequence on a Typic Haplustert as influenced by organic manure and inorganic fertilizer N were investigated in a field experiment. The application of farmyard manure at 4 to 16 $Mg{\cdot}ha^{-1}\;y^{-1}r^{-1}$ along with fertilizer nitrogen at 50 or 180 $kg{\cdot}ha^{-1}$ increased the mean soil microbial biomass from 1.12 to 2.05 fold over unmanured soils under soybean-wheat system. Irrespective of organic and chemical fertilizer N application, the soil microbial biomass was maximum during the first two months at active growing stage of the crops and subsequently declined with crop maturity. The mean annual microbial activity was significantly increased when manure and chemical fertilizer at 8 $Mg{\cdot}ha^{-1}$ and 50/180 N $kg{\cdot}ha^{-1}$, respectively were applied. The C turnover rate decreased by 47 to 72 % when the level of farmyard manure was increased from 4 to 8 and 16 $Mg{\cdot}ha^{-1}$. There were significant correlations between biomass C, available N, dehydrogenase, phosphatase and yield of the crops.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.184-184
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• 2022

This study was aimed to evaluate the antioxidant activity and bioactive property of extracts from a purple-colored wheat variety 'Arriheuk' (Triticum aestivum L.) wheatgrass, affected by light emitting diode irradiation (LED). The wheatgrass was cultivated for 10 days after sowing in a growth chamber under the following LED conditions: R1B1 (Red:Blue = 1:1), R7B3 (Red:Blue = 7:3), and R3B7 (Red:Blue = 3:7). We examined antioxidant activity of the hot water extracts of wheatgrass using DPPH and ABTS free radicals scavenging assays. At the concentration of 10,000 ㎍/ml, the extract from R1B1 showed the highest DPPH free radical scavenging activity(79.29%), but its ABTS free radical scavenging activity was the lowest(32.0%). To evaluate bioactive property of the wheatgrass, we examined the change of natural killer (NK) cell activity affected by the wheatgrass extracts in vitro. At the concentration of 500㎍/ml, NK cell activity was most highly enhanced by the extract from R1B1(181.6%), and the activity was 176.1% (R7B3) and 144.6%(R3B7), respectively. These results suggest that the functional property of 'Arriheuk' wheatgrass would be enhanced by LED irradiation condition.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.293-293
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• 2022

This study is to develop new wheat breeding material through wide hybridization with wild species harboring useful characteristics such as salt, heat, and drought tolerance. Leymus mollis, wild rye was used to improve wheat genetic quality. L. mollis, is a perennial plant harboring tolerance against salt, heat, and drought because L. mollis distributes on the seaside. The F₁ hybrids were produced by crossing between common wheat (Triticum aestivum L., Chinese Spring) and L. mollis. Genomic in situ hybridization revealed that the F₁ hybrids have L. mollis genome. For the evaluation of salt and drought tolerance, seeds from the F₂ were used. Under 2% NaCl solution, the F₃ wheat-Leymus addition plants with salt tolerance showed more tillering and longer roots than other F₃ plants without salt tolerance. Also, the F₃ plants with salt tolerance showed better shallow-rooted than other F₃ plants without salt tolerance. Finally, the F₃ plants with salt tolerance made seed-setting under 2% NaCl condition, but other F₃ plants without salt tolerance were not. Under drought conditions, the F₃ plants with drought tolerance showed longer culm and spike length than other F₃ plants without drought tolerance and even those of Chinese Spring under well-water conditions. We evaluated and selected the F₃ plants with salt or drought tolerance for generation advancement.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.4
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• pp.343-370
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• 2023

Developing improved wheat varieties is vital for global food security to meet the rising demand for food. Therefore, assessing the genetic diversity across wheat genotypes is crucial. This study examined the diversity of 168 durum wheat and 47 common wheat collections from 54 different countries using twelve agro-morphological parameters. Geumgang, a prominent Korean common wheat variety, was used as a control. Both qualitative and quantitative agronomical characteristics showed wide variations. Most durum wheats were shown to possess dense spikes (90%), while common wheats showed dense (40%) or loose (38%) spikes, with yellowish-white being the dominant spike color. The majority of the accessions were awned regardless of wheat type, yellowish-white being the main awn color. White or red kernels were produced, with white kernels dominating in both common (74%) and durum (79%) wheats. Days to heading (DH) and days to maturity (DM) were in the ranges of 166-215 and 208-250 days, respectively, while the culm length (CL), spike length (SL), and awn length (AL) were in the ranges of 53.67-163, 5.33-18.67, and 0.50-19.00 cm, respectively. Durum wheats possessed the shortest average DH, DM, and SL, while common wheat had the longest CL and AL (p < 0.05). Common wheats also exhibited the highest average one-thousand-kernel weight. Hierarchical cluster analysis, aided by principal component analysis, grouped the population into seven clusters with significant differences in their quantitative variables (p < 0.05). In conclusion, this research revealed diversity among common and durum wheat genotypes. Notably, 26 durum wheat and 17 common wheat accessions outperformed the control, offering the potential for developing early-maturing, high-yielding, and lodging-resistant wheat varieties.

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1300-1307
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• 2007

This study assessed the possible role of different traits in selected plant growth-promoting rhizobacteria (PGPR) for improving wheat growth and yield under natural conditions. Rhizobacteria exhibiting 1-aminocyclopropane-1-carboxylate (ACC)-deaminase activity were isolated and screened for their growth-promoting activity in wheat under axenic conditions. Five isolates belonging to Pseudomonas and one Burkholderia caryophylli isolate that showed promising performances under axenic conditions were selected and characterized for in vitro ACC-deaminase activity, chitinase activity, auxin production, P solubilization, and root colonization. These isolates were then used as inocula for wheat cultivated under natural conditions in pot and/or field trials. Significant increases in root elongation, root weight, tillers per pot, 1,000-grain weight, and grain and straw yields were observed in response to inoculation with PGPR in the pot trials. Inoculation with these PGPR was also effective under field conditions and increased the wheat growth and yield significantly. However, the efficacy of the strains was inconsistent under the axenic, pot, and field conditions. Pseudomonas fluorescens ($ACC_{50}$), which exhibited a relatively high in vitro ACC-deaminase activity, chitinase activity, auxin production, and P solubilization and more intensive root colonization, was the most efficient isolate under the field conditions. Therefore, these results demonstrated that ACC-deaminase activity is an efficient parameter for the selection of promising PGPR under axenic conditions. However, additional traits of PGPR, including auxin production, chitinase activity, P solubilization, and root colonization, are also important for selecting PGPR as biofertilizers.

• The Plant Pathology Journal
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• v.31 no.3
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• pp.245-251
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• 2015

Alternative hosts increase the difficulty of disease management in crops because these alternate hosts provide additional sources of primary inoculum or refuges for diversity in the pathogen gene pool. Agropyron cristatum (crested wheatgrass), Bromus inermis (smooth bromegrass), Pascopyrum smithii (western wheatgrass), Stipa viridula (green needlegrass), and Thinopyrum intermedium (intermediate wheatgrass), commonly identified in range, prairie, verge, and soil reclamation habitats, serve as additional hosts for Pyrenophora tritici-repentis, the cause of tan spot in wheat (Triticum aestivum L.). A. cristatum (five lines), B. inermis (seven lines), P. smithii (four lines), S. viridula (two lines), and T. intermedium (six lines) were tested for their reactions to 30 representative P. tritici-repentis isolates from races 1-5. Plants were grown until the two-three-leaf stage in a greenhouse, inoculated individually with the 30 isolates, held at high humidity for 24 h, and rated after 7 days. All lines developed lesion types 1-2 (resistant) based on a 1-5 rating scale. Also, leaves from an additional plant set were infiltrated with two host selective toxins, Ptr ToxA as a pure preparation and Ptr ToxB as a dilute crude culture filtrate. All lines were insensitive to the toxins. Results indicate that these grass hosts have a limited or nonsignificant role in tan spot epidemiology on wheat in the northern Great Plains. Additionally, the resistant reactions demonstrated by the grass species in this research indicate the presence of resistance genes that can be valuable to wheat breeding programs for improving wheat resistance to P. tritici-repentis.