• Korean Journal of Plant Resources
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• v.26 no.6
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• pp.695-700
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• 2013

Flax (Linum usitatissimum L.) has been used for the only edible oil in Korea. We carried out the field experiment in order to investigate the possibly combined effects of mepiquat chloride (MC) and trinexapac-ethyl (TE) on oil composition, lignan content, seed yield and endogenous gibberellins content of flax cultivar. Plant growth retardants mepiquat chloride (300 and 600 ppm) and trinexapac-ethyl (100, 200 and 300 ppm) were foliar-sprayed to flax plant at 50days after seeding. The plant height was decreased in the combination of mepiquat chloride 600 ppm with trinexapac-ethyl 100, 200 and 300 ppm. Mepiquat chloride treatment combined with trinexapac-ethyl observed the highest response on seed yield, followed by mepiquat chloride 300 ppm with trinexapac-ethyl 100 ppm, mepiquat chloride 300 ppm with trinexapac-ethyl 200 ppm and mepiquat chloride 300 ppm with trinexapac-ethyl 300 ppm. Lignan content was increased in all of the combination treatments. It concludes that the combination of mepiquat chloride 300 ppm with trinexapac-ethyl 300 ppm will be useful to increasing oil and lignan content in flax plants.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.318-327
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• 2015

BACKGROUND: Trinexapac-ethyl is a plant growth regulator (PGR) that inhibits the biosynthesis of plant growth hormone (gibberellin). It is used for the prevention of lodging, increasing yields of cereals, and reducing mowing of turf. The experiment was conducted to establish a determination method for trinexapac-ethyl and its metabolites trinexapac in agricultural products using LC-MS/MS.METHODS AND RESULTS: Trinexapac-ethyl and trinexapac were extracted from agricultural products with methanol/ distilled water and the extract was partitioned with dichloromethane and then detected by LC-MS/MS. Limit of detection(LOD) was 0.003 mg/kg and limit of quantification(LOQ) was 0.01 mg/kg, respectively. Matrix matched calibration curves were linear over the calibration ranges (0.01-1.0 mg/L) for all the analytes into blank extract withr²> 0.997. For validation purposes, recovery studies were carried out at three different concentration levels (LOQ, 10LOQ, 50LOQ,n=5). Recoveries of trinexapacethyl and trinexapac were within the range of 73.6-106.9%, 72.7-99.2%, respectively. The relative standard deviations (RSDs) were less than 9.0%. All values were consistent with the criteria ranges requested in the CODEX guideline(CAC/GL 40, 2003).CONCLUSION: The proposed analytical method was accurate, effective and sensitive for trinexapac-ethyl and trinexapac determination and it can be used to as an official method in Korea.

• Korean Journal of Weed Science
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• v.13 no.1
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• pp.19-25
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• 1993

This study was conducted to evaluate effect of trinexapac-ethyl{4-(cyclopropyl-${\alpha}$-hydroxy-methylene)-3, 5-dioxocyclohexan carboxylic acid ethyl-ester} for lodging prevention, growth pattern of several organs and yield in machine transplanted rice with 10-day-old seedling. Elongation rate of the second internode affected the most largely by trinexapac-ethyl was 33-52, 35-56 and 42-53% of check in application of 12, 7 and 5days before heading, respectively and, culm elongation was reduced 27-34, 20-29 and 20-25% in application of the same time. Lodging in field was decreased by treatment of trinexapac-ethyl compared with check, Ripened grain ratio and yield were increased about 3-7 and 7-17% by trinexapac-ethyl, respectively.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.156-160
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• 2010

This study was performed to provide useful information for kentucky bluegrass management during summer by application of plant growth regulator, Trinexapac-ethyl. Visual quality, shoot density and chlorophyll contents of treatment blocks were significantly different from those of control during summer by application of Trinexapac-ethyl. The turfgrass density of treatment was increased of 4ea/$10\;cm^2$, especially about 5ea/$10\;cm^2$ during the growth retarded period of June and July. Chlorophyll contents index and visual quality of kentucky bluegrass were improved by application of Trinexapac-ethyl during summer, too. In addition, the occurrence of foliage in rainy and high temperature season was less than that of control. However, there was no significant difference in the root length of Kentucky Bluegrass. Meanwhiles, mowing height of kentucky Bluegrass was suppressed by 38% at 2 WAT week after treatment and that there was no significant growth of treatment at 4 WAT. In this experiment, turfgrass quality was significantly better than that of control during July, even though trinexapac-ethyl wasn't applied at all in July. Consequently, periodic treatment of trinexapac- ethyl from spring would be very important to promote the turfgrass visual quality during summer which is unfavorable season on the growth of kentucky bluegrass. And it is possible to reduce mowing times at least 30% for 2 weeks by one application of Trinexapac-ethyl 0.02~0.03 ml/$m^2$ in kentucky bluegrass fairway. Additively, trinexapac- ethyl treatment can be helpful environmentally by cutting down the fertilizers and pesticides in golf course.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.248-252
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• 1999

This study examined the effects of growth retardants and midsummer drainage on lodging-related characters in direct seeding rice culture on flooded paddy surfaces. Treatments included in this study were continuous flooding, two time midsummer drainages (TTD), and inabenfide or trinexapac-ethyl application after TTD. Culm length was reduced most by 11.4cm in trinexapac-ethyl treatment after TTD, followed by 4.1cm in inabenfide treatment after TTD compared to TTD treatment. A significant reduction in culm length occurred at the 3rd internode in inabenfide treatment applied at 40 days before heading (DBH), while trinexapac-ethyl application at 8 DBH reduced the 3rd>2nd>1st internode. The culm diameter of the 4th internode was increased by the application of trinexapac-ethyl following TTD compared to TTD treatment. Thickness of culm wall was significantly increased by trinexapac-ethyl but not by inabenfide applications. Lodging index was the highest in continuous flooding and the lowest in trinexapac-ethyl treatment after TTD. Subsequently, field lodging did not occur in plots treated by trinexapac-ethyl. Rice plants subjected to midsummer drainage or growth retardants showed higher ripened grain ratio and 1000-grain weight, resulting in higher rice yield. These results indicate that trinexapac-ethyl, which can be applied at the relatively later growth stage after proper judgement of lodging occurrence, could be a useful tool for reducing lodging in direct seeding rice culture on flooded paddy fields.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.287-294
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• 2009

This study conducted to evaluate the growth characteristics of creeping bentgrass in summer after application of the plant growth regulator, Trinecapac-ethyl, and these data may provide basic information to golf course turf maintenance. The results showed that the shoot density of creeping bentgrass was increased an average density of 1.7 $ea/cm^2$ with the trinexapac-ethyl application, especially about 2 $ea/cm^2$ during the growth retarded period of June and July. The root length increased also in June and August. The visual quality was improved significantly with trinexapac-ethyl treatment all the experimental periods, moreover, the effect was significant by reducing a summer decline stress of creeping bentgrass during the warm and humid period of summer. The green speed was significantly improved by this growth regulator treatment and those effect was prominent during stressed season of late June to mid July. Overall of the result, we found that shoot density, visual quality and green speed of bentgrass green were improved by trinexapac-ethyl treated from early growing season of spring and these effects were continued during summer. It should be very beneficial to manage the bentgrass green in stressed season. In future, the possibility and efficiency of mixture with fungicides and/or fertilizers might be needed. The spring green-up test with trinexapac-ethyl will be followed in next spring.

• Asian Journal of Turfgrass Science
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• v.14 no.1
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• pp.273-280
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• 2000

This study was conducted to evaluate an efficiency of plant growth regulator trinexapacethy(Primo) on the growth response of bentgrass and the change to soil water content in bentgrass green. Based on the results of the study, the following results were observed. 1. During four weeks after treatment, the visual color and turfgrass density of all the treated plots with trinexapac-ethyl(Primo) were more improved rather than without. Two treatments trinexapac-ethyl /$0.02mL\m^2$ and $0.04mL/\m^2$ were more favorable than other treatments. 2. It suggested that optimum rate to reduce the bentgrass growth and to increase the turfgrass density was the trinexapac-ethyl $0.04mL/\m^2$. 3. For six weeks after treatment, all treated plots were not significantly different (P<0.05) in turfgrass root length and root dry weitht. 4. In the treated plots with trinexapac-ethyl $0.04mL/\m^2$ for 25days in bentgrass green, soil water consumption was approximately 35% to 40% compare to the non-treated control.

• Korean Journal of Plant Resources
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• v.24 no.6
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• pp.696-701
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• 2011

The aim of this field experiment was to investigate the possible effects of mepiquat chloride (TE) and trinexapac-ethyl (MC) on oil composition, seed yield and endogenous gibberellins content of flax cultivar. Foliar application of plant growth retardants mepiquat chloride (0.897, 1.794 and 2.691 kg a.i. $ha^{-1}$) and trinexapac-ethyl (0.756, 1.512 and 2.668 kg a.i. $ha^{-1}$) had significantly increased seeds ripening rate and seed yield. In contrast, plant height was decreased by foliar application of MC and TE. The application of MC significantly increased seed oil yield (730 kg $ha^{-1}$ by 27.0%) compared to the control. Seed and oil yield, and unsaturated fatty acids (oleic acid, linoleic acid and linolenic acid) were increased by foliar application of MC.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.186-190
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• 1999

The objective of this study was to ,determine trinexapac-ethyl [4-(cyclopropyl- $\alpha$ -hydroxy-me-thylene)-3,5-dioxocyclohexanecarboxylic acid ethyl ester] effects on lodging-related traits of rice varieties treated at different application rates and times. Trinexapac-ethyl WP was applied at the rate of 10, 20 and 30g ai/l0a to Heukjinjubyeo and Daeanbyeo, respectively. Effects of trinexapacethyl varied greatly with application time as well as application rate with little differences between rice varieties. As the application rate increased, lodging index, center of gravity and exsertion of panicle above flag leaf decreased, whereas breaking strength and phytotoxicity increased. Exsertion of panicle was greatly influenced by the application time with a little difference by application rate. The most effective application time of trinexapac-ethyl based on lodging index and total internode length (Ist-4th node) was 2DBH(days before heading) in Heukjinjubyeo and 17DBH in Daeanbyeo, resulting in reducing total internode length by 12.9 and 9.7cm, respectively. A significant reduction was found at the 1st, 2nd, and 3rd internodes in Heukjinjubyeo, while at the 2nd and 3rd internodes in Daeanbyeo. No significant difference was found among treatments in the panicle length of both rice varieties but the panicle fresh weights of Daeanbyeo were significantly greater in all trinexapac-ethyl treatments compared with that of the control when measured 20 days after heading. Consequently, trinexapacethyl treatment significantly improved lodging-related traits of rice plants without affecting rice yield and yield components.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.2
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• pp.176-179
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• 1999

Trinexapac-ethyl[ 4-(cyclopropyl- $\alpha$ -hydroxy-methylene)-3,5-dioxocyclohexane carboxylic acid ethylester] is a growth-retardant for plants by inhibiting a key step in biosynthesis of GA. A treatment of trinexapacethyl generally induces a reduction in vegetative growth and also inhibits heading. In addition, the trinexapacethyl was known to enhance the freeze-tolerance in annual bluegrass, however, the mechanism is not known yet. One possible reason for the enhanced freeze-tolerance may be the antifreeze protein known to be accumulated in intercellular space of the leaf during cold acclimation. In order to see the possible in-duction of the synthesis of antifreeze proteins by trinexacpacethyl, the apoplastic proteins extracted from Kentucky bluegrass treated with trinexapacethyl were analyzed by SDS-PAGE and the presence of the antifreeze protein was observed. In addition, western analysis showed the identity of the protein induced by both a cold acclimation and a trinexapacethyl treatment. It appears that an enhanced freeze-tolerance of the turf grass by trinexapacethyl is due to the synthesis and/or accumulation of the antifreeze protein similar to the enhanced freeze tolerance induced by cold acclimation.