• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.6
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• pp.123-132
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• 2016

The study was to introduce the revegetation measures for the improvement of the landscape in October, 2012 on the slope; two and four years after the introduction, the slope was examined to see the status of the revegetation. The result is as follows. The result from examining the soil on the slope, the soil was Loamy sand; which infers that the soil is in a poor condition for improving the scenery. Examining the characteristics of the vegetation-base materials, the soil acidity was pH 6.4 to 6.7; and the soil hardness was 21 to 24mm (hardness quotient of soil), this can be considered a favourable condition, which has no effect on the growth and development of plants. When it comes to the discovery of the species in the area, Lotus corniculatus var., Silene armeria L., Papaver rhoeas, Trifolium pratense L. developed and grew in spring after two years. And in the summer, Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense were founded. And there were Lotus corniculatus var., Dianthus chinensis L., Aster yomena, Coreopsis tinctoria, Trifolium pratense L. in the autumn. According to the examination conducting after four years, Lotus corniculatus var., Dianthus chinensis L., Silene armeria, Cosmos bipinnatus, Coreopsis drummondii L., Papaver rhoeas, Trifolium pratense L. were founded in the spring, and Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense grew in the summer. Lotus corniculatus var., Dianthus chinensis L., Astragalus sinicus, Aster yomena, Coreopsis tinctoria, Trifolium pratense L. were discovered in the autumn. Among the exotic species founded in the area, were Festuca arundinacea, Lolium perenne, Festuca rubra. When it comes to the protection of vegetation and dominance, Lotus corniculatus var., Metaplexis japonica, Coreopsis drummondii L. are the dominant species among the spring plants; and Lotus corniculatus var., Trifolium repens L., Erigeron annuus (L.) Pers. were the dominant plants in the summer. In the autumn, Lotus corniculatus var., Coreopsis drummondii L., Poa pratensis appear to be the dominant plants. The colonies of Pinus densiflora and Castanea crenata var. were formed around the South Sejong IC. Part of the area is occupied by the colony of Alnus hirsuta Turcz. ex Rupr. In terms of shrub, there were Indigofera pseudo-tinctoria, Robinia pseudoacacia, and Rosa polyantha var. genuina NAKA. As ground cover plants, Sasa borealis, Rubus crataegifolius Bunge were founded.

• International Journal of Oral Biology
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• v.44 no.3
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• pp.81-88
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• 2019

Trifolium pratense leaves (red clover) has been used in Oriental and European folk medicine for the treatment of whooping cough, asthma, and eczema, and is now being used to treat and alleviate the symptoms, such as hot flushes, cardiovascular health effects that occur in postmenopausal women. However, relatively little scientific data is available on the physiological activity of this plant. Therefore, in this study, we investigated the anti-cancer activity of T. pratense leaves using methanol extract of T. pratense leaves (MeTP) on human FaDu hypopharyngeal squamous carcinoma cells. MeTP inhibited the viability of FaDu cells by inducing apoptosis through the cleavage of procaspase-3, -7, and -9 and poly (adenosine diphosphate ribose-ribose) polymerase (PARP), downregulation of Bcl-2, and upregulation of Bax, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live & dead assay, 4'6-diamidino-2-phenylindole stain, fluorescence-activated cell sorting analysis, and Western blot analysis. In addition, colony formation was slightly inhibited when FaDu cells were treated with a non-cytotoxic concentration (0.125 mg/mL) of MeTP and almost completely inhibited when cells were treated with 0.25 mg/mL MeTP. Collectively, these results indicate that MeTP induced cell apoptosis via caspase- and mitochondrial-dependent apoptotic pathways, and inhibited colony formation of cancer cells in FaDu human hypopharyngeal squamous carcinoma cells. These findings suggest MeTP should be considered for clinical development as a chemotherapeutic option in oral cancer.

• Proceedings of the Korean Society of Crop Science Conference
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• 1991.05a
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• pp.42-43
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• 1991

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.437-442
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• 2004

Forage legumes are being used as an important source of protein for dairy farm as well as nitrogen fertilizer. This experiment was conducted to evaluate forage production and quality of five legumes at Cheonan Yonam College Experimental Livestock Farm for 1 year(2001-2002). Five legumes were crimson clover (Trifolium incarnatum L.) 'Tibbee', hairy vetch(Vicia villosa Roth) 'Common', red clover(Trifolium pratense L.) 'Kenland', persian clover(Trifolium resupinatum L.) 'Leeton', alfalfa(Medicago sativa L.) 'Vernal'. All legumes were shown cold hardiness of 8 rating or higher except crimson clover(7 rating). Flowering stage was observed 23th of April for crimson clover, 13th of May for hairy vetch, 3rd of May for red clover, and 12th of May for alfalfa. But persian clover didn't at harvest. Dry rnatter(DM) of crimson clover(17.6%) was the highest among legumes. In DM and total digestible nutrients(TDN) yields, crimson clover and hairy vetch were higher than those of other legumes. The crude protein of hairy vetch(20.5%) was the highest among legumes. In neutral detergent fiber(NDF) and acid detergent fiber(ADF), persian clover was the lowest(35.0% and 25.0%). Calculations of relative feed value(RFV) based on NDF and ADF of legumes were over 125(Prime degree) except hairy vetch. RFV and TDN of persian clover were the highest among legumes. According to the results of this study, crimson clover and hairy vetch are excellent in forage yield. Persian clover is superior in forage quality among legumes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.127-132
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• 1999

No comprehensive study of yield and quality losses of forage legumes harvested and cured in spring has been conducted in Korea, therefore, this experiment was carried out to gain information on yield and quality, losses of alfalfa(Medicgo sativa L.), crimson clover (Trifolium incarnatum L.), and red clover(Trifolium pratense L.) during field curing in spring(mid-May). Alfalfa was highest in dry matter content of 15% at harvest, while crimson clover and red clover had 12.4 and 11.5%, respectively. Species differences for the dry matter content were maintained and consistent trends were observed during the field curing. Alfalfa took seven days to reach dry matter content over 80%, while both crimson clover and red clover took eight days. Crimson clover and alfalfa tended to show a higher leaf-stem ratio than red clover based on dry matter, but red clover showed a higher leaf-stem ratio than alfalfa and crimson clover based on crude protein content. Losses in dry matter of alfalfa from leaf shattering were higher than crimson clover and red clover, and similar trends were detected losses in crude protein for three different legumes. Fresh legume forages averaged higher in clude protein(CP), total digestible nutrients(TDN), and relative feed value(RFV), while dry legume forages averaged lower in acid detergent fiber(ADF) and neutral detergent fiber(NDF). Results of the experiment indicate that hay curing in spring affects crude protein concentration more than ADF and NDF, and this is due, in part, to leaf shattering caused by field operations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.3
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• pp.199-207
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• 1996

This experiment was canied out to determine the effects of seeding method and seeding ratio in mixed cropping of forage rye and red clover on dry matter yield, nutritional yield and chemical characteristics of soil. The main treatment was two seeding methods(drilling and broadcasting) and the sub treatment was six seeding ratios between rye and red clover(T1; 150kg : 0 kg/ha, T2; 120 : 3, T3; 90 : 6, T4; 60 : 9, T5; 30 : 12, T6; 0 : 15). The experiment was performed at the College of Natural Science of Kon-Kuk University in Chungju in 1992. The results were summarized as follows; 1. Protein content was increased by increasing the seeding ratio of red clover, but content of NDF and ADF were decreased. 2. The seeding method did not affects significantly the dry matter yield, but broadcasting showed higher dry matter yield than drilling in the same seeding ratio. Especially, T3 of broadcasting was the highest yield of 13, 172 kg/ha The T3 of broadcasting and T4 of drilling showed hlghly crude protein yield of 1, 268 kgha and 1, 252 kg/ha, respectively. 3. Comparing the each seeding ratio of two seeding method, total nitrogen yield of shoot and root of drilling were higher than broadcasting. Especially, T2 and T4 of drilling were the highest in total nitrogen yield. 4. There were more increase in organic matter and nitrogen contents but decrease in K, Ca of soil by increasing the seeding ratio of red clover than soil before experiment. In the seeding methods, broadcasting was an effect of soil improvement.

• Korean Journal of Organic Agriculture
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• v.22 no.1
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• pp.129-145
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• 2014

To evaluate the potential of Hypochoeris radicata L. for the development of environment-friendly organic materials, its growth characteristics, allelopathic effects and antifungal activity was investigated. The growth characteristics of H. radicata L. was investigated by measuring comparative number of shoot and flower, and the diameters of clones in mowing areas and non-mowing areas, respectively. As a result, the number of shoot and flower, and diameters of clones of H. radicata L. grown in mowing areas were much higher than those grown in non-mowing areas. Water extracts of H. radicata L. inhibited seed germination, and shoot, root and root hair growth of 14 test plants including Trifolium pratense, Festuca myuros, Bidens bipinnata and finally reduced heir biomass remarkably. The inhibitory effect of the extract was different depending on the kind and the part of tested plants. The extracts showed high antifungal activity against Pythium spp. and Phytophthora However, it showed comparably less antifungal activity against Rhizoctonia solani than Pythium spp. and Phytophthora. In conclusion, cutting H. radicata L. resulted increase of its vegetative and seed propagation and helped it to form large colony. Also it had an effect on growth of microbes and germination and growth of other plants. Therefore H. radicata L. holds the competitive dominant position in plant ecosystem in Jeju Island and it can be used as candidate of environment-friendly organic materials.

• Korean Journal of Plant Resources
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• v.35 no.1
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• pp.71-99
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• 2022

This study was conducted to investigate the vascular flora of Masan, located in Goseong-gun, Gangwon-do, and to comprehend the distribution of remarkable plants such as rare and Korea endemic plants and invasive alien plants. The survey was carried out the distribution of vascular plants 11 times per season from 2010 to 2018. A total of 619 taxa in 93 families, 328 genera, 529 species, 6 subspecies, 78 varieties and 6 forms were identified on mt. Masan. Among them, Korean endemic plants are 21 taxa including Saussurea diamantica Nakai and are plants designated by the Korea Forest Service are 20 taxa counting Saxifraga octopetala Nakai. Invasive alien plants were classified a total of 35 taxa inclusive of Trifolium pratense L. (WS), Aster pilosus Willd. (SS), Coreopsis lanceolata L. (SR), Rumex obtusifolius L. (SC) and Lindernia dubia (L.) Pennell (CS). The naturalization rate was 5% and the urbanization index was 10.0%, respectively. Although this study identified the diverse distribution of major plants such as rare and endemic plants around Masan, it was confirmed that invasive alien plants, which pose a great threat to forest biodiversity conservation and promotion, spread and distributed in a specific section Therefore, it is urgent to establish a conservation strategy and countermeasures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.293-304
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• 1997

A field experiment was conducted at the forage experimental plots, Seoul National University, Suweon h m 1995 to 1996 to determine the effect of legume residues as a N source and N fertilizer on corn (Zea mays L.) silage yield, N uptake, and availability of inorganic N in the soil. Corn was grown following (i) red clover (Trifolium pratense L.), (ii) crimson clover (Trifolium incarnatum L.), (iii) alfalfa (Medicago sativa L.) and (iv) winter fallow. The plots were split into two rates of fertilizer N (0 and 90kg Nlha) in a split-plot experimental plan. Compared with fallow treatment, legumes depleted soil water in the surface 15cm at corn planting by 17 to 26%. As a result, corn emergence was markedly delayed with legume residues by 8 to 11 days. Corn silage DM yield was significantly reduced in the presence of legume residues by 2.0 to 3.4 and 1.5 to 2.5 tonlha compared with winter fallow treatment at 0 and 90kg fertilizer Nlha, respectively, but no significant difference in the corn DM yield was found between legume residue treatments. There was an overall tendency for increased corn yields with 90kg fertilizer Nlha compared to Okg fertilizer Nlha, although not all yield increases were significantly greater. The corn yield response to applied N suggests that a source of N from legume residues was not sufficient for a succeeding corn crop. There was significantly more N (P

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.3
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• pp.119-123
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• 1989

레드 클로버의 葉肉細胞로 부터 原形質體의 裸出에 影響을 미치는 要因들을 調査하여 다음과 같은 結果를 얻었다. 原形質體의 裸出에 使用된 酵素는 Cellulase, Macerozyme, Driselase 및 Pectolyase였으며 그 中 1%의 Cellulase ONOZUKA R-10과 0.5%의 Macerozyme R-10의 組合에서 가장 좋은 結果를 얻었다. 裸出된 原形質體의 適正 膨壓을 維持하기 爲하여 Mannitol의 濃度를 0.3~O.8M의 범위에서 6단계로 조정하였을 때, 0.7M에서 가장 높은 收率을 얻을 수 있었다. 原形質體 裸出을 爲한 酵素溶液의 pH는 5.6에서 가장 좋은 結果를 나타내었으며, 材料로 쓰인 레드 클로버 일을 4가지 크기로 區分하여 裸出實驗한 結果, 작은 잎보다 큰 잎에서 收率이 높은 傾向이었다. 酵素處理 後 裸出된 原形質體의 정제에는 0.8M의 Sucrose가 가장 適當하였다.