• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.292-298
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• 2014

Tridemorph is a systemic morpholine fungicide for crops. The objective of this study was to develop reliable and sensitive analytical method for determination of tridemorph residues in tea samples for ensuring the food safety. Tridemorph residues in samples were extracted with acetonitrile after hydration, partitioned with saline water, and then purified using an aminopropyl ($NH_2$) SPE cartridge. The purified samples were detected and quantified using LC-ESI-MS/MS. The linear detection limits for tridemorph ranged from 0.02 to $1.0mgL^{-1}$ with a correlation coefficient of 0.9999. The method was validated using tea samples spiked with tridemorph at different concentration levels (0.02 and $0.05{\mu}gmL^{-1}$). The average recovery ranged between 75.0 and 84.7% with relative standard deviations less than 10%. The LOD and LOQ were 0.01 and $0.02mgL^{-1}$, respectively. The developed method was applied successfully to the identification of tridemorph in real tea samples obtained from different sources, and tridemorph was not detected in any of the samples. The results show that the developed analytical method is accurate and suitable for tridemorph determination in tea samples.

• Journal of Food Hygiene and Safety
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• v.32 no.4
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• pp.290-297
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• 2017

The purpose of this study was developed for the determination of tridemorph in agricultural commodities samples. Tridemorph residues in samples were extracted with acetonitrile, partitioned with saline water, and then purified using and aminopropyl ($NH_2$) SPE catridge. The purified samples were quantified and confirmed via liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). Matrix-matched calibration curves were linear over the calibration ranges (0.005~2.5 ng) into a blank extract with $r^2$ > 0.999. The limits of detection and quantification were 0.001 and 0.005 mg/kg, respectively. The average recovery ranged between 75.9% and 103.7% at different concentration levels (LOQ, 10 LOQ, 50 LOQ, n = 5) with relative standard deviations (RSDs) less than 9.0%. An interlaboratory study was conducted to validate the method by Korea Advanced Food Research Institute. The average recovery ranged between 87.0% and 109.2% at different concentration levels (LOQ, $10{\times}LOQ$, $50{\times}LOQ$, n = 5) with relative standard deviations (RSDs) less than 8.0%. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL40, 2003) and Food Safety Evaluation Department guidelines (2016). The results prove that the developed analytical methods is accurate, effective and sensitive for tridemorph determination.

• The Plant Pathology Journal
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• v.18 no.2
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• pp.68-73
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• 2002

The influence of fungicidal spray was assessed on powdery mildew epidemics caused by Erysiphe polygoni D.C. and on yield-attributing characters of mungbean [Vigna radiata (L.) Wilczek]. Mildew attack adversely affected the yield-attributing characters of mungbean and exhibited negative significant correlation with pod length (-0.57), pods/plant (-0.74), pod weight (-0.68), 100 seeds weight (-0.69), 100 seeds swell weight (-0.59), and seed germination (-0.71). These characters had direct or indirect effects on grain yield, which was also adversely affected due to mildew attack (-0.89). Powdery mildew was significantly retarded due to the single spray of carbendazim (0.05%) at 30-day-old crop, where the apparent rate of infection (r) was minimum at 0.0095/ unit/day and with low (11.44%) powdery mildew intensity. Other fungicides like tridemorph (0.075%) and penconazole (0.05%) were equally effective against the mildew disease where the disease intensity was less than 20% and the values of r were 0.0134 and 0.039/unit/day respectively, as compared with the control at 0.267/unit/day. Fungicide spray influenced the yield-attributing characters besides controlling the disease. Such effects were more pronounced in carbendazim (0.05%)-treated plots due to its phytotonic nature where pod length (7.59cm), pods/plant (29.75), pod weight (8.16 g), 100 grain weight (3.94 g), and swell weight of 100 seed (9.49 g) were maximum resulting to the highest yield (480 kg/ha) as compared with that of control (224 kg/ha). Spray of carbendazim also improved seed germination (74.5%). Spray of other fungicides like carbendazim with copper oxychloride in 1:1 ratio, tridemorph (0.075%), and penconazole (0.05%) was equally effective against powdery mildew of mungbean. These fungicides also exhibited positive effects on yield-attributing characters of the crop and finally increased yield. These systemic fungicides were more effective in controlling powdery mildew disease of V. radiata in the rainy season compared with wettable sulphur.