• 제목/요약/키워드: Trichosporon sp.

검색결과 4건 처리시간 0.016초

Effect of Glucose Concentration on the Production of Erythritol by Trichosporon sp.

  • Park, Jin-Byung;Seo, Byung-Cheol;Kim, Jung-Ryul;Pek, Un-Hua;Park, Yong-Kun
    • Journal of Microbiology and Biotechnology
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    • 제8권5호
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    • pp.543-546
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    • 1998
  • The effect of glucose concentration on the production of erythritol by Trichosporon sp. was mainly studied. The specific growth rate and production rate of erythritol gave the highest values of $0.23 h^{-1}\; and\; 4.2 g/\ell/h,\; respectively,\; on\; 100 g \; glucose/\ell$ of medium. The conversion yield of erythritol during the exponential phase and the stationary phase was constantly maintained at 19% and 51 %, respectively, while the glucose concentration in the medium varied from 100 g/$\ell$ to 400 g/$\ell$. The maximum overall erythritol conversion yield of 47% was obtained when the glucose concentration in the medium was 400 g/$\ell$. It corresponded to a 74% increase compared with the 100 g/$\ell$ glucose medium. The diauxy growth of this microbe was also observed. It grew exponentially consuming glucose, then after the second lag phase, biomass slowly increased using glycerol and erythritol.

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Purification and Characterization of Lipase from Trichosporon sp. Y-11and Its Use in Ester Synthesis of Unsaturated Fatty Acids and Alcohols

  • Song, Xin;Qu, Yinbo;Shin, Dong-Hoon;Kim, Eun-Ki
    • Journal of Microbiology and Biotechnology
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    • 제11권6호
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    • pp.951-956
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    • 2001
  • A 28-kDa extracellular lipase (pI 8.7) was purified to homogeneity from the culture supernatant of Trichosporon sp. Y- 11 by mmonium sulfate precipitation, DEAE-Sephadex A-50, Bio-Gel P-30, CM- Sephadex C-50, and Bio-Gel P- 10 chromatographies. The purified enzyme exhibited a specific activity of $2,741{\;}{\mu}mol/min/mg$ based on the hydrolysis of triolein, and the optimal hydrolysis activity was dentified at pH 8.0 and $40^{\circ}C$. The enzyme activity was inhibited by $Ag^+$ and enhanced by $Fe^{2+}$, $Fe^{3+}$, $Mg^{2+}$, $Mn^{2+}$, and $Li^{+}$. The enzyme activity exhibited for the hydrolysis of both tributyrin and trilinolein. The ester synthesis of unsaturated fatty acids with various alcohols catalyzed by the purified lipase in a nonaqueous medium or microaqueous system was also investigated. The esterification activity of the lipase increased with an increase of the carbon chain length in the alcohol. The synthesis rate of linoleic acid and oleyl alcohol was the highest with an optimal temperature and pH of $40^{\circ}C$ and 8.0, respectively. The water content and agitation also affected the esterification activity of the lipase.

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Streptomyces sp. LAM-593이 생산하는 수용성 항진균성 항생물질 (Solumycin : A water-soluble antifungal antibiotic from Streptomyces sp. LAM-593)

  • 이동희;박승림;권태종;정호권
    • Applied Biological Chemistry
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    • 제34권2호
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    • pp.180-186
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    • 1991
  • 토양에서 분리한, 수용성 항진균성물질(solumycin)을 생산하는 Streptomyces sp. LAM-593의 배양액으로부터 butanol 추출, alumina와 2회의 Sephadex LH-20 column chromatography 등의 방법으로 물질을 정제하고 여러가지 성질을 조사한 결과는 다음과 같다. 본 물질은 silica gel TLC에서 단일 spot를 나타내었으며 ethanol-ammonia water-water (8:1:1). butanol-ethanol-water (5:1:4 및 5:2:2), 50% methanol계에서의 Rf치는 각각 0.24, 0.46, 0.57, 0.84였고, 물 methanol, acidic aq. butanol 등에 잘 용해하였으며 Fehling과 Molish 반응에서 양성인 342, 361. 380, 404nm에서 peak를 나타내는 heptaene계 물질이었다. 그리고 Candida, Cryptococcus, Saccharomyces, Trichophyton, Trichosporon 등의 진균에 대해서는 항균력이 컸으나 여러가지 세균에 대해서는 활성이 거의 없었다.

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Dynamics of fungal community during silage fermentation of elephant grass (Pennisetum purpureum) produced in northern Vietnam

  • Vu, Viet Ha;Li, Xiyang;Wang, Mengyuan;Liu, Rongmei;Zhang, Guojian;Liu, Wei;Xia, Baixue;Sun, Qun
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권7호
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    • pp.996-1006
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    • 2019
  • Objective: This study aimed to gain deeper insights into the dynamic changes in spoilage fungi populations during fermentation and the influence of traditional additives on silage quality. Methods: Elephant grass (Pennisetum purpureum) was prepared without any additive (control), and with the addition of 0.5% salt, and 0.5% salt-0.2% sugar mixture. The fungal community was then determined using a classic culturing method and high-throughput sequencing at 0, 5, 15, and 60 days after ensiling. Results: The results showed that the fungal community of elephant grass silage varied significantly between the natural fermentation without any additive and the two additive groups. The diversity and relative abundance of spoilage molds in the control group were much higher than those in the two treatment groups (p<0.05). Three species of yeasts (Candida sp., Pichia sp., Trichosporon sp.) and four spoilage molds (Fusarium sp., Aspergillus sp., Muco sp. and Penicillin sp.) were the predominant fungi in elephant grass during natural fermentation from 0 to 60 days, which were found to be significantly decreased in salt and sugar additive groups (p<0.05). Meanwhile, the diversity and relative abundance of undesirable molds in the 0.5%-salt additive group were the lowest among all groups. Conclusion: Adding salt and sugar, particularly 0.5% salt, is a promising effective approach to reduce the amount of undesirable fungi thus, improving the silage quality of elephant grass in northern Vietnam.