• The Korean Journal of Mycology
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• v.20 no.2
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• pp.144-148
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• 1992

This experiment was carried out to investigate on the flora and cardinal wild mushroom in Tricholoma matsutake producing sites at the Namweon area. The results were as follows: Flora of Tricholoma matsutake producing sites were prevailed over at tree layer (Pinus densiflora S. et. Z.), shrub layer (Rhododendora mucronulatum TURCZ., Quercus serrata THUNB.) and herb layer (Carex humilis LEYSS.). Piedominant wild mushrooms were Boletaceae (Suillusplacidus Bon.) and Ramariaceae (Ramaria botrytis Fr.)

• The Korean Journal of Mycology
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• v.19 no.2
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• pp.109-119
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• 1991

The vertical distribution of the fungal population for the soil samples from two sites of producing and nonproducing of Tricholoma matsutake, song-yi mushroom, were examined at Yang­yang and Myung-joo, Gangweon province. By the dilution plate method, a total number of propagu­les of fungi per gram of soil was observed to be low at the song-yi producing sites but high at the song-yi nonproducing sites under the communities of Pinus densiflora. The tendency of the number of fungal propagules were decreased with the increasing vertical depth. In the incuhation method at $42^{\circ}C$, six genera and nineteen species of the fungi were isolated from two sites; Aspergillus fumigatus, Acremonium sp., Talaromyces stipitatus, Penicillium lilacinum, P. oxalicum and Westerdykella multispora. The most dominant species by this method was A. fumigatus. From heat treatment method at $70^{\circ}C$, seven genera and nineteen species were isolated; Aspergillus fumigatus, Alternaria alternata, Neurospora sitophila and Mucor sp.. In the ethanol treatment method, one genera and one species was isolated Mortierella sp.. From the three isolation methods, it was found that the total number of the soil fungi and the frequency of species appeared were the highest at the soil of upper layer whereas the lowest at the soils of lower layer in its vertical distribution.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.89-98
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• 2016

Endophytes play an important role in the growth and development of the host. However, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. We studied the bacteria associated with fruiting bodies of Tricholoma matsutake picked from seven main T. matsutake-producing areas in Sichuan, China, by barcoded pyrosequencing. About 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and archaea, and 361-797 operational taxonomic units were observed at a 97% similarity level. The bacterial community was always both more abundant and more diverse than the archaeal community. UniFrac analysis showed there were some difference of bacterial communities among the samples sites. Three bacterial phyla, Proteobacteria, Bacteroidetes, and Firmicutes, were dominant in all samples. Correlation analysis showed there was a significant correlation between some soil properties and bacterial community associated with T. matsutake. This study demonstrated that the bacteria associated with T. matsutake fruiting bodies were diversified. Among these bacteria, we may find some strains that can promote the growth of T. matsutake.

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.203-213
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• 1991

From 1986 to 1990, the fifty-three sites (shiros) of the five places known as an areas of the pine-mushroom production in Kyoung Sang Do were visited, investigating the biology or ecology of pine-mushrooms. Physical or ecological characteristics of the areas producing pine-mush­rooms was similar to those reported in Japan, but not similar to in this regardings; The ages of pine trees, the understory plant flora of the places producing the pine-mushrooms and the soil types. The areas of pine-mushroom productions are divided to two typical areas, the inland and coastal areas. Ecological differences between two areas were found, The understory plant flora and different soil types. Three kinds of soil were, by the naked eyes, were found from our observa­tions of Kyoung Sang Do, but not based on soil analysis. The pine-mushrooms observed in Kyoung Sang Do were related to the root of the pine trees, but not confirmed to be ectomycorrhizally related to the pine trees. The mycelia isolated form the different places were compared with the degradation of phenolic compounds and culture methods, but were not clearly distinguished. The productions of pine-mushroom and several methods for the pine mushroom were suggested under four years' observations.