• Journal of Microbiology
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• 제44권2호
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• pp.200-205
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• 2006

The tributyltin (TBT)-resistant bacterium, Pseudomonas aeruginosa 25W, which was isolated in seawater from the Arabian Sea, was subjected to transcriptome analysis in the presence of high concentrations of TBT. Only slight effects were observed at TBT concentration of $50{\mu}M$, but exposure to $500{\mu}M$ resulted in the upregulation of 6 genes and the downregulation of 75. Among the 75 downregulated genes, 53% (40 out of 75) were of hypothetical function, followed by 14 transcriptional regulation- and translation-associated genes. The results of this study indicated that although the 25W strain was highly resistant to TBT, high concentrations of TBT result in toxic effect on the transcriptional and translational levels. The target genes likely belong to a specific category of transcription- and translation-associated genes rather than to other gene categories.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.168-168
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• 2002

A clear association between occupational tributyltin (TBT) exposure and testicular cell line, TM3 has been observed in mouse. In the present study, we have investigated cytotoxic effects of TBT on Significant cytolethality was observed in TM3 cells in a time- and concentration-dependent manner when measured by MTT assays. (omitted)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.169-169
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• 2002

The present was performed to identify the effects of tributyltin oxide (TBTO) in the immature mice testes. 3-week-old male ICR mice were orally administrated on one time basis of TBTO dose of 0 (Vehicle control, VC), 30 (TBTO 30 mg/kg, T30), 60 (TBTO 60 mg/kg, T60) per each one.(omitted)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.138-138
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• 2003

Tributyltin (TBT) is also recognized as an endocrine disrupter. Organotin compounds such as TBT are widely used as agricultural biocides, and for antifouling paint of ship bottoms and of fishing nets. In this study, we investigated the role of nur 77 in induction of apoptosis in TBT-induced leydig cells.(omitted)

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.169-169
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• 2002

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.168-168
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• 2002

• Bulletin of the Korean Chemical Society
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• 제32권1호
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• pp.356-358
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• 2011

• 환경생물
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• 제22권1호
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• pp.177-183
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• 2004

유기주석화합물인 tributyltin chloride (TBTC), tributyltin oxide (TBTO)와 triphenyltin chloride (TPTC)를 넙치 간장으로 만든 미크로좀에 in vitro적으로 노출시켜서 이들 화합물의 대사에 관여하는 mixed function oxidase (MFO) 중 cytochrome P450 (CYP) 농도와 7-ethoxyresorufin deethylase (EROD) 활성의 변화를 조사하였으며, 또한 in vivo 실험에서는 TPTC를 넙치에게 복강주사(7.5 mg $kg^{-1}$ BW)하여 간장의 MFO (CYP농도, NADPH cytochrome c 환원효소 활성, NADH chtochrome b5 환원 효소 활성, EROD 활성) 반응을 경시적으로 조사하였다. 그 결과, in vitro에서는 TBTC, TBTO 및 TPTC가 모두 CYP 농도와 EROD 활성을 저해하였으며, 저해력은 TPTC가 가장 컸고 이어서 TBTO, TBTC의 순이었다. 유기주석화합물의 노출농도와 노출시간과 비례하면서 저해정도가 커졌으며, 특히 EROD활성의 저해는 노출농도에 크게 의존적이었다. 그리고 in vivo실험에서도 유기주석 화합물은 CYP농도, NADPH cytochrome c 환원효소 활성, NADH cytochrome b5 환원효소 활성, EROD 활성을 억제하였다. EROD 활성은 오염물질에 의한 반응이 민감하고 재현성도 있어 바람직한 측정지표로 이용될 수가 있을 것이다.

• Fisheries and Aquatic Sciences
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• 제9권2호
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• pp.64-69
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• 2006

Cytochrome P450 (CYP1A) gene expression in the liver and sex steroid levels in plasma were investigated in olive flounder (Paralichthys olivaceus) exposed to tributyltin (TBT) and benzo[a]pyrene (BaP). We constructed a cDNA library and cloned a 230-base sequence encoding partial CYP1A DNA. The CYP1A gene expression level was estimated using northern blotting. Hepatic CYP1A mRNA levels in fish injected with BaP at 10 mg/kg body weight (b.w.) increased for 48 h after injection. However, fish injected with both BaP and TBT at 10 mg/kg b.w. showed no significant changes in CYP1A mRNA level after 48 h. Plasma concentrations of testosterone and $17{\beta}$-estradiol were not significantly different in males and females injected with BaP and TBT. We suggest that TBT-induced suppression of BaP bioactivity should be interpreted with caution in biomonitoring field studies.

• Journal of the korean society of oceanography
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• 제33권3호
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• pp.90-99
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• 1998

Butyltin and phenyltin residues were quantified in seawater and biota of the Chinhae Bay System, Korea in 1995. Butyltin compounds were detected in all seawater and biota samples, whereas phenyltin compounds were found only in the biota samples. Tributyltin (TBT) concentrations in seawater ranged from < 8-35 ng Sn/l. Tributyltin concentrations in Crassostyea gigas and Thais clavigera ranged from 95-885 and 23-414 ng Sn/g, respectively, Triphenyltin(TPhT) concentrations in each species ranged 155-678 and 46-785 ng Sn/g, respectively. Spatial distribution of TBT was closely related to boating and dry-docking activities. However, spatial distribution of TPhT was not consistent with that of TBT. The biological concentration factor for TBT in C. gigas was about 25000 that is four times greater than that of T. clavigera. Butyl- to phenyltin concentration ratio was greater than one in C. gigas, but that in T. clavigera was less than one. Major tissues of C. gigas also showed different accumulation patterns for butyl- and phenyltin compounds. Furthermore, 19 and 28% of total body burdens of TBT and TPhT were found in gonadal mass of C. gigas just prior to spawning.