• Parasites, Hosts and Diseases
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• 제32권3호
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• pp.149-156
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• 1994

주민들의 장내 기생충 감염상황 조사와 구충을 목적으로 1994년 3월 경상남도 거창군 고제면 주민 116명의 대변을 수거하여 포르말린-에테르 집란법으로 1번 검사하였다 장흡충 충란 양성자들에게 프라지콴텔을 투여하여 충체를 회수하였다. 주민들의 충란 양성율은 11.2%(13례)이었으며 간흡충 3.4%(4례), 메타고니무스 5.2%(6례), 호르텐스극구흡충 9.5%(11례)이었다. 7명의 호르텐스극구흡충 양성자를 치료하여 평균 65(6-227)마리의 호르텐스극구흡충을 수집하였다 그중 1례에서 이전고환극구흡충 3마리를 수집하였고, 다른 1례에서 수세미이형흡충 한마리를 수집하였다. 상복부 팽만감, 무른변 설사, 변비가 감염자의 주된 위장관 증상이었다. 경남 거창군 고제면을 호르텐스극구흡충 유행지의 하나로 추가한다. 이 조사의 이전고환극구흡충증은 국내 제6례, 수세미이형흡충증은 국내 제 5례이다.

• Parasites, Hosts and Diseases
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• 제58권6호
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• pp.701-707
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• 2020

A survey was performed to investigate the infection status of zoonotic helminth larvae in fish from a local market of North Dagon District in Yangon City, Myanmar. A total of 486 fish in 13 species were collected 8 times from December 2015 to December 2019. All fish were transported under ice to a laboratory in Korea and examined for helminth larvae using artificial digestion method. Larval gnathostomes and metacercariae of more than 8 zoonotic trematode species, i.e., Opisthorchis viverrini, Haplorchis taichui, H. pumilio, H. yokogawai, Centrocestus spp., Stellantchasmus falcatus, Pygidiopsis cambodiensis, and Procerovum sp., were detected. Larval gnathostomes were found in 58 (16.0%) out of 362 fish of 6 species, with mean intensity of 2.8 per fish infected. Metacercariae of O. viverrini were detected in 10 (2.9%) out of 349 fish of 5 species, with mean intensity of 16.9 per fish infected. Metacercarial prevalences of 4 intestinal flukes, H. taichui, H. pumilio, H. yokogawai, and Centrocestus spp., were 16.8%, 26.0%, 12.5%, and 15.0% in the positive fish species, respectively, and mean metacercarial intensity was 63.3, 26.8, 86.2, and 8.7 per fish infected. Metacercariae of S. falcatus and P. cambodiensis were detected only from the mullet, Chelon macrolepis. Metacercariae of Procerovum sp. were found in Channa striata and Anabas testudineus. Collectively, it was confirmed that the fish were infected with gnathostome larvae and metacercariae of O. viverrini and intestinal flukes in Yangon City, Myanmar.

• Journal of Ecology and Environment
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• 제30권1호
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• pp.97-99
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• 2007

Waders inhabiting an area in which A. tyosenense is endemic were examined to determine if they serve as reservoir hosts for the parasite. Of the waders examined, 4 individuals from 2 species were found to be hosts for 18 to 1820 adult worms. Common and velvet scoters, which are known to be the final hosts of A. tyosenense, are rare in the infected areas, while waders are prevalent and prey on the bivalves known to be intermediate hosts of A. tyosenense. Thus, from the experimental results and circumstances presented herein, we conclude that waders are definitive and reservoir hosts of A. tyosenense in nature.

• Parasites, Hosts and Diseases
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• 제48권4호
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• pp.343-346
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• 2010

The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.

• Parasites, Hosts and Diseases
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• 제60권5호
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• pp.367-370
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• 2022

Fascioliasis is a parasitic infection caused by liver flukes. Although several cases have been reported in Korea, phylogenetic analysis of isolates is lacking. In this study, a 66-year-old woman with right upper quadrant (RUQ) abdominal pain was diagnosed as fascioliasis involving abdominal muscle by imaging study. She received praziquantel treatment, but symptoms were not improved. Lateral movement of the abscess lesion was followed. Trematode parasite was surgically removed from the patient's rectus abdominis muscle. The fluke was identified as Fasciola hepatica based on sequence analysis of 18S rDNA. To determine the phylogenetic position of this Fasciola strain (named Korean Fasciola 1; KF1), the cox1 gene (273 bp) was analyzed and compared with the genes of 17 F. hepatica strains isolated from cows, sheep, goats, and humans from various countries. Phylogenetic analysis showed that KF1 was closely related with the isolates from China goat.

• 한국임상수의학회지
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• 제37권5호
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• pp.242-248
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• 2020

An intestinal strigeiid trematode, Apharyngostrigea pipientis, was detected from Ardea cinerea jouyi, Egretta intermedia and Nycticorax nycticorax between July, 2006 and June, 2020 in Daejeon metropolitan city and Kangwon-do, Republic of Korea. It was observed with a light and scanning electron microscope (SEM), and molecularly analyzed with 18S rRNA genes. The infection rate of the strigeiid trematode was 25.9% (7/27). The body is cylindrical in whole mount, greatly elongated in outline and shows a strong dorsal curvature. The overall measurements are: length 4.1-4.8 mm; fore-body 1.00-1.20 mm by 0.8-0.9 mm; hind-body 3.2-3.6 mm by 0.43-0.46 mm. The body is covered by a relatively thick cuticle, devoid of spines. The oral sucker leads into a short esophagus that is devoid of a muscular pharynx. The holdfast organ is enormously developed. The vitelline follicles distributed dorsal wall of fore-body and hind-body. This strigeiid trematode especially differs from other species of genus Apharyngostrigea in morphology. Although these A. pipientis are several morphological distinctions from Apharyngostrigea cornu, the partial sequence of 18S rRNA showed the sequence homology of A. pipientis (99.9%) and A. cornu (99.8%), and this result revealed their conspecific relationship between A. cornu and A. pipientis. This is the first report of A. pipientis in the Republic of Korea.

• 한국수산과학회지
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• 제13권4호
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• pp.173-178
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• 1980

1980년 1월부터 동반 10월까지 군산 근교 미면 내초도에서 채집한 백합Meretrix lusoria, 바지락 Tapes japonica, 맞조개 Seten strictus을 조사하여 흡충류의 유충인 Bacciger harengulae의 cercaria의 높은 기생율을 보았다. 이 cercaria는 S자형으로 활발히 운동한다. 꼬리에는 양쪽에 27쌍의 강모(seta)을 가지며, 1개의 강모(setae)는 6개의 가락(조)으로 되어 있다. 체내에는 구흡반, 맹장, 흡복반과 2개의 정소가 뚜렷히 보이고, 큰 V자형의 관상 배설낭내에는 작고 큰 과립이 충만되고, 염세포식은 2[(3+3)+(3+3)]=24이다. 현재까지 보고된 유사한 ceraria를 비교하니 Cercaria pectinata와 같았다. 제2중간숙주를 거쳐 결숙주에 성충을 얻었으므로, Bacciger harengulae의 cercaria라 동정하였다. 내초도에서 채집한 패류중 맛조개, 바지락, 백합의 순으로 이 기생법의 기생률이 높았으나, 이들 조개류는 모두 2-3년생인큰 것이 많이 기생되어 있었다.

• 대한의생명과학회지
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• 제12권4호
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• pp.329-335
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• 2006

Echinostoma hortense (E. hortense) is an intestinal trematode with the highest infection rate in South Korea. However, the immune response against E. hortense infection has not been explained well. In the present study, we investigated the effect of treatment with cyclosporin A (CsA) and histamine receptor antagonists on the cytokine expression and mucosal goblet cells in E. hortense-infected C3H/HeN mice. The alteration of cytokine mRNA expression ($TNF-{\alpha},\;IL-l{\beta},\;IL-4\;and\;IL-5$), intestinal worm recovery rate and goblet cell responses were measured weekly from 0 to 5 weeks post-infection (P.I.) in the control and the following three drug-treated groups: CsA, hydroxyzine and cimetidine. Compared with the control group, the expression of $TNF-{\alpha}$, IL-4 and IL-5 mRNAs decreased in the CsA- and hydroxyzine-treated groups, but only IL-4 mRNA expression did in the cimetidine-treated group. Worm recovery rate was significantly increased in the drug-treated groups. Mucosal goblet cells and their mucin response significantly decreased in the CsA-treated group (P<0.01), but significantly increased in the cimetidine- (P<0.05) and hydroxyzine- (P<0.01) treated groups. These data suggest that CsA treatment inhibits production of Th1- and Th2-type cytokines which are necessary for the worm expulsion. Histamine receptor increases goblet cells and their mucin activation, although it remains to be elucidated whether it directly affects the worm expulsion period of E. hortense in C3H/HeN mice.

• Parasites, Hosts and Diseases
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• 제56권4호
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• pp.335-339
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• 2018

This study was conducted to investigate the prevalence of intestinal parasites among students from Goseonggun, Gangwon-do and Hadong-gun, Gyeongsangnam-do as typical low and high endemic counties. From May to July 2017, a total of 2,033 fecal samples were collected at 27 elementary, 10 junior high, and 8 high schools from 2 counties and examined by the Kato-Katz technique for egg-positive surveys (Collection rate: 37.02% [2,033/5,492]). Of the participants examined, 13 (0.64%) were found to harbor eggs of 3 parasitic species, Trichuris trichiura, Clonorchis sinensis and Metagonimus yokogawai. Based on the regional distribution, the egg-positive rate in Goseong-gun was 0% (0/550) and that in Hadong-gun was 0.88% (13/1,483). The positive rates for C. sinensis, M. yokogawai, and T. trichiura in Hadonggun were 0.20% (3/1,483), 0.61% (9/1,483), and 0.07% (1/1,483), respectively. The present survey showed that the prevalence of parasitic infection among students is currently very low even in remote, previously endemic areas, and the present status of parasitic diseases can be summarized as some transmission of fish/food-borne trematodes. Thus, it is necessary to carefully, continuously monitor the trematode infection status, particularly of C. sinensis and M. yokogawai.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.641-645
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• 2015

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.