• Journal of the Korea Furniture Society
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• v.20 no.1
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• pp.52-60
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• 2009

Joyangru, which is located in Woodo mountain in Chuncheon, Korea, was a gate tower of Monsogak. There are little historical records about Joyangru except of the 20th-century records. To study about Joyangru history, a dendrochronological analysis was conducted. We identified also the species of woods, We took 87 samples of wood elements for the species analysis and 13 samples for the dendrochronological analysis. We found 78 hard pines(Diploxylon), 4 Douglass-fir, 3 exotic hard pines(Pinus ponderosa type) and 1 Shorea sp.(Dipterocarpaceae). In the dendrochronological analysis, 2 floor flames were dated in 1887 (with complete sapwood) and 2 beams in 1884. We concluded that Joyangru was reconstructed just after 1888, most likely in 1890 together with Monsogak.

• Korean Journal of Organic Agriculture
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• v.6 no.1
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• pp.35-43
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• 1997

For the purpose of acquiring microbial agents that can be utilized to billogically control the major airborne disease to apple trees, such as canker(Valsa ceratosperma), the effective microorgaisms were isolated, tested for antagonistic activity to the pathogen causing major disease to apple trees and identifed. Screening of more than 3, 000 species of microorganisms collected in nature for them antagonistic action to the pathogen, Valsa ceratosperma causing disease to apple tree resulted in selection of effective species. Out of the 11 species, one species designated as CAP141 demonstrated outstanding activity. The bacterial strain, CAP141 exerted antagonistic efficiency of 65% on Valsa ceratosperma. The CAP141 was identified as a bacterial strain to Bacillus subtilis based on morphology, culture conditions, and physio-biochemical characteristics.

• Journal of Forest and Environmental Science
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• v.37 no.2
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• pp.163-167
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• 2021

Mushroom survey and collection were conducted in the Dong Hua Sao National Biodiversity Conservation Area (DHSNBCA), which is located about 30 km east of Pakse and about 10 km south of the town of Paksong, Champasak Province of Lao-PDR, from from at the early July to October in 2019. During this periods, mushroom specimens were collected from 6 different locations, and then they were identified and classified into 103 species, 74 genera, 37 families, 15 orders and 6 classes by morphological and molecular analyses. The mushrooms belongs to Ascomycota were classified into 10 species, 6 genera, 4 families, 3 orders and 2 classes, while those belongs to Basidiomycota were classified into 92 species, 67 genera, 32 families, 11 orders, and 3 classes, respectively. In addition, 1 species of Myxomycota was also identified. Among these mushrooms, the most species-rich families are Marasmiaceae (17.3%), Polyporaceae (13.1%), Mycenaceae (8.9%), Agricaceae (8.3%), Xylariaceae (6.5%), Auriculariaceae (4.8%), Ganodermtaceae (3.6%), Dacrymycetaceae (3.0%), Meruliaceae (3.0%), Russulaceae (3.0%) and comprised 71.4% of the total specimens identified.

• Journal of the Korean Wood Science and Technology
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• v.44 no.6
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• pp.897-902
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• 2016

This study was conducted to identify wood species from main pillars of Daeungjeon Hall, the main building of Jeonghyesa Temple located in Suncheon, Jeollanamdo Province in Korea. It was known that this temple had been built in the $8^{th}$ century by Hyeso, a national master in the Unified Silla period, and was designated as Treasure No. 804 in 1984 due to its historic and academic values. The results of wood species identification showed that main pillars from Daeungjeon Hall of the Jeonghyesa Temple were made of hard pine, Korean fir (Abies spp.) and Zelkova tree (Zelkova spp.). In addition to the wood species identification, floor plan documentation was also done to help identify what wood species had been used for main pillars. These results could be applied and utilized for the conservation and restoration of Daeungjeon Hall in future. This study may help with the authenticity of conservation works.

• Journal of the Korea Furniture Society
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• v.20 no.4
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• pp.274-280
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• 2009

The purpose of this study was to identify the species of coffin woods excavated at Mundangdong in Gimcheon and to date this coffin by using tree-ring method. All coffin woods were identified as red pines, most possibly, Pinus densiflora S. et Z. Tree-ring dating provided absolute years of 3 among 19 coffins. Both I-9 and II-22 coffins were estimated to be made in the mid-seventeenth century, and I-65-1 in the mid-sixteenth century. Others possessed too few rings to be dated.

• The Journal of the Korean Society for Microbiology
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• v.34 no.6
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• pp.561-571
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• 1999

Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the ${\beta}$ subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.

• Korean Journal of Remote Sensing
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• v.24 no.3
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• pp.267-272
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• 2008

Tree species-specific estimates with spacebome high-resolution imagery improve estimation of forest biomass which is needed to predict the long term planning for the sustainable forest management(SFM). This paper is a contribution to develop crown distinguishing coniferous species, Pinus densiflora and Pinus koraiensis, from QuickBird imagery. The proposed feature analysis derived from shape parameters and first and second-order statistical texture features of the same test area were compared for the two species separation and delineation. As expected, initial studies have shown that both formfactor and compactness shape parameters provided the successful differentiating method between the pine species within the compartment for single crown identification from spaceborne high resolution imagery. Another result revealed that the selected texture parameters - the mean, variance, angular second moment(ASM) - in the infrared band image could produce good subset combination of texture features for representing detailed tree crown outline.

• Journal of Forest and Environmental Science
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• v.35 no.4
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• pp.268-271
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• 2019

Mushroom survey and collection were conducted in the Phousabous National Protected Area (PNPA), which is located at the Northeastern area 230km from the capital city of Lao-PDR, Vientiane, from April 2017 to September 2018. During this periods, mushroom specimens were collected from 8 different locations, and then they were identified and classified into 284 species, 142 genera, 56 families, 20 orders and 7 classes by morphological and molecular analyses. The mushrooms belongs to Ascomycota were classified into 10 species, 5 genera, 5 families, 4 orders and 3 classes, while those belongs to Basidiomycota were classified into 274 species, 137 genera, 51 families, 16 orders, and 4 classes, respectively. Among these mushrooms, the most species-rich families are Boletaceae (16.06%), Polyporaceae (10.12%), Russulaceae (9.94%), Amanitaceae (7.68%), Agaricaceae (4.54%), Marasmiaceae (4.36%), Cortinariaceae (3.66%), Entolomataceae (3.66%), Mycenaceae (2.79%), Xylariaceae (2.44%), Physalacriaceae (2.09%), Omphalotaceae (2.09%), Hydnangitaceae (2.09%), Lyophyllaceae (1.92%), Tricholomataceae (1.75%) and comprised 75.19% of the total specimens identified.

• Journal of Microbiology
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• v.41 no.4
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• pp.312-319
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• 2003

In an attempt to develop a method for rapid and accurate identification of six Vibrio species that are clinically important and most frequently detected in Korea, 16S rDNA restriction fragment length polymorphism (RFLP) of Vibrio type strains, as well as environmental isolates obtained from the Korean coastal area, was analyzed using ten restriction endonucleases. Digestion of the 16S rDNA fragments amplified by polymerase chain reaction (PCR) with the enzymes gave rise to 2~6 restriction patterns for each digestion for 47 Vibrio strains and isolates. An additional 2~3 restriction patterns were observed for five reference species, including Escherichia coli, Aeromonas hydrophila, A. salmonicida, Photobacterium phosphoreum, and Plesiomonas shigelloides. A genetic distance tree based on RFLP of the bacterial species correlated well with that based on 16S rDNA sequences. The very small 16S rDNA sequence difference (0.1%) between V. alginolyticus and V. parahaemolyticus was resolved clearly by RFLP with a genetic distance of more than 2%. RFLP variation within a species was also detected in the cases of V. parahaemolyticus, V. proteolyticus, and V. vulnificus. According to the RFLP analysis, six Vibrio and five reference species were assigned to 12 genotypes. Using three restriction endonucleases to analyze RFLP proved sufficient to identify the six pathogenic Vibrio species.

• Korean Journal of Plant Taxonomy
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• v.46 no.2
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• pp.163-174
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• 2016

Gnetum (Gnetaceae, Gnetales) is a gymnosperm genus with ca. 35 species distributed in tropical forests around the world. Due to its dioecious habit and lack of diagnostic characters from vegetative tissue, the identification of Gnetum species is not easy without seeds or reproductive structures. To identify and verify their phylogenetic positions, we applied DNA barcoding to Cambodian Gnetum collections gathered between 2010 and 2015, with previously designed cp matK gene primers. We newly sequenced partial matK sequences from 72 Gnetum collections, 43 out of 72 from Cambodia, and analyzed 115 Gnetum accessions using the neighbor-joining method. The resulting neighbor-joining tree categorized Cambodian Gnetum samples into three clades of species: G. macrostachyum, G. montanum, and G. aff. gracilipes. The recognition of G. aff. gracilipes in Cambodia is reported here for the first time. Taxonomic information for the three recognized Cambodian Gnetum species is provided and the benefits of the taxonomic reevaluation assisted by DNA barcoding are emphasized in this work.