• Journal of fish pathology
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• v.24 no.3
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Animal cells and systems
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• v.7 no.2
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• The Transactions of the Korean Institute of Power Electronics
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• v.11 no.4
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• pp.313-320
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• 2006

In this paper, a design approach of LC trap filter for output side harmonic reduction of single phase NPC multilevel inverter is proposed, and THD of the output voltage and harmonic FFT of the output current are analyzed. The proposed filter consists of a conventional LCR filter cascaded with an LC trap filter and it is tuned to inverter switching frequency. A NPC multilevel inverter inverter is used an inverter system for high power application and DSP(TMS320C31) is used for the controller. The effectiveness of the proposed system confirmed through simulation and experimental results.

• Proceedings of the SAREK Conference
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• 2009.06a
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• pp.465-470
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• 2009

This study investigated the water saving technology for the toilet stool system in a water closet. Since in 21st century water would be the prospected factor to measure the wealth of a country instead of the oil, water saving technologies in the toilet stool in a closet system is getting more important than the cleaning stool and the reducing noise performances. According to the statistics in Korea the percentage of using water in toilet is about 27% to the total amount of water used. Therefore, this study theoretically approached the possible amount of water saving and suggested the new variable position straight trap device in the toilet stool system.

• Current Optics and Photonics
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• v.5 no.3
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• pp.220-228
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• 2021

To improve the measurement accuracy of a solar-radiation observer instrument, aiming at the problem of multiorder-stray-light interference caused by the diffraction of the flat-field concave grating in the spectroscopic system, straylight suppression methods for different forms of optical traps are studied. According to the grating surface-scattering distribution-function model, the bidirectional scattering distribution function (BSDF) of a dust-polluted surface and the flat-field concave grating's transition area of the spectroscopic system is calculated, and a Lyot stop with blade baffle is designed to suppress this kind of stray light. For diffraction multiorder stray light, based on the theory of light-energy transmission, a design for precise positioning of the trench optical trap is proposed. The superiority of the method is verified through simulation and actual measurement. The simulation results show that in a spectroscopic system approximately 160 mm × 140 mm × 80 mm in size, the energy of the stray light is reduced by one order of magnitude by means of the trench optical trap and Lyot stop, and the number of beams is reduced from 5664 to 1040. The actual measurements show that the stray-light-suppression efficiency is about 69.4%, which is effective reduction of the amount of stray light.

• Journal of Electrical Engineering and Technology
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• v.9 no.2
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• pp.635-642
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• 2014

The space charge behaviors of oil-paper insulation affect the stability and security of oil-filled converter transformers of traditional and new energies. This paper presents the results of the electrical aging of oil-impregnated paper under AC-DC combined voltages by the pulsed electro-acoustic technique. Data mining and feature extractions were performed on the influence of electrical aging on charge dynamics based on the experiment results in the first stage. Characteristic parameters such as total charge injection and apparent charge mobility were calculated. The influences of electrical aging on the trap energy distribution of an oil-paper insulation system were analyzed and discussed. Longer electrical aging time would increase the depth and energy density of charge trap, which decelerates the apparent charge mobility and increases the probability of hot electron formation. This mechanism would accelerate damage to the cellulose and the formation of discharge channels, enhance the acceleration of the electric field distortion, and shorten insulation lifetime under AC-DC combined voltages.

• Korean journal of applied entomology
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• v.58 no.2
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• pp.143-149
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• 2019

This study was conducted to examine the attractiveness of yellow sticky trap (YST) for insect pests by the angle of inclination of the trap surface. In strawberry farms with high bed system, YSTs were installed to attract insect pests in vertical direction with one surface, horizontal direction with upper and under surface, and angle of $45^{\circ}$ with upper and lower sticky surface. Thrips (Frankliniella occidentalis in dominance) and Sciaridae (Bradysia agrestis in dominance) species were more attracted on upper surface of $45^{\circ}$ angle trap, vertical surface and upper surface of horizontal trap than on lower surface of $45^{\circ}$ angle trap or horizontal trap. Cicadellidae (Empoasca vitis in dominance) species were caught more on vertical surface and upper surface of $45^{\circ}$ angle trap than on other traps. There were no specific trend in capture of aphid (Aphis gossypii in dominance) and white fly (Trialeurodes packardi in dominance) species among traps, probably because of a low density of the pest species.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.47 no.4
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• pp.300-315
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• 2011

The investigation for the catch and species composition in the deep sea of the East Sea was done to search marine organisms using some kinds of traps with small commercial fishing vessel near Goseong, Korea from 2008 to 2009. The experiments was carried out with some different traps at the depth of 200m, 400m, 700m and 1,000m. In 2008, the amount of catch with rectangular, cone and drum net traps was 91.4kg with twelve trips and the proportion of catch with rectangular and cone traps was high. Dominant species caught were Aniwan whelk (57%), snow crab (12%), hunchback sculpin (7%) and red snow crab (5%). The catch of Aniwan whelk was done well with rectangular and cone traps at the depth of 700-1,000m. Snow crab was mainly caught with rectangular and cone traps at the shallower depth of 200m, while red snow crab caught a lot with cone trap at the deeper depth of 700m. In 2009, the amount of catch with rectangular, cone and round traps was 92.4kg with nine trips. The proportion of catch with round trap was higher than that with cone trap. Dominant species caught were snow crab (67.1%), Aniwan whelk (14.8%) and northern shrimp (9.8%). Snow crab was caught well with round trap at the depth less than 400m.

• The Journal of Fisheries Business Administration
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• v.50 no.1
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• pp.1-15
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• 2019

Offshore trap fishery managers have payed in advance to attract excellent fishing crew in the minium grantee lay system. This practice can intensify competition among fishery management bodies and increase the burden of fishery manager about income tax and four social insurance programs. The purpose of this study is to identify the problems of advance payment practice in terms of fishery manager in offshore trap fishery. The main results of this study are as follows. First, under the TYPE 1 model, which is the current wage payment manner, there is a risk that fishery manager will not able to return the prepayment paid in advance when the income of fishery management bodies decrease. Second, the TYPE 2 or the TYPE 3 model which reduces or abolishes advance payment can alleviate the burden of fishery manager relative to the deduction of redemption amount, incentive to induce artificial expenses, income tax and four social insurance programs. In conclusion, advance payment practice in offshore trap fishery is placing a heavy burden on the fishery manager. In order to solve these problems in the future, we need to find solutions through similar case studies.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.60 no.2
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• pp.161-169
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• 2024

This study employs Bayesian network analysis to quantitatively evaluate the risk of incidents in trap boats, utilizing accident compensation approval data spanning from 2018 to 2022. With a dataset comprising 1,635 incidents, the analysis reveals a mortality risk of approximately 0.011 across the entire trap boat. The study significantly identifies variations in incident risks contingent upon fishing area and fishing processes. Specifically, incidents are approximately 1.22 times more likely to occur in coastal compared to offshore, and the risk during fishing processes outweighs that during maintenance operations by a factor of approximately 23.20. Furthermore, a detailed examination of incident types reveals varying incidence rates. Trip/slip incidents, for instance, are approximately 1.36 times more prevalent than bump/hit incidents, 1.58 times more than stuck incidents, and a substantial 5.17 times more than fall incidents. The study concludes by providing inferred mortality risks for 16 distinct scenarios, incorporating fishing areas, processes, and incident types. This foundational data offers a tailored approach to risk mitigation, enabling proactive measures suited to specific circumstances and occurrence types in the trap boat industry.