• Korean Journal of Environmental Agriculture
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• v.29 no.1
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• pp.27-32
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• 2010

This study was conducted to reclassify Anryong series based on the second edition of Soil Taxonomy and to discuss the formation of Anryong series distributed on the mountain foot slope. Morphological properties of typifying pedon of Anryong series were investigated and physico-chemical properties were analyzed according to Soil survey laboratory methods manual. The typifying pedon of Anryong series has brown (7.5YR 4/4) loam Ap horizon (0-22 cm), strong brown (7.5YR 4/6) cobbly clay loam BAt horizon (22-35 cm), strong brown (7.5YR 4/6) cobbly clay loam Bt1 horizon (35-55 cm), reddish brown (5YR 5/4) cobbly clay loam Bt2 horizon (55-82 cm), and brown (7.5YR 5/4) cobbly clay loam Bt3 horizon (82-120 cm). The typifying pedon has an argillic horizon from a depth of 22 to 120 cm and a base saturation (sum of cations) of less than 35% at 125 cm below the upper boundary of the argillic horizon. It can be classified as Ultisol, not as Alfisol. It has udic soil moisture regime, and can be classified as Udult. Also that meets the requirements of Typic Hapludults. It has 18-35% clay at the particle-size control section, and have mesic soil temperature regime. Therefore Anryong series can be classified as fine loamy, mesic family of Typic Hapludults, not as fine loamy, mesic family of Ultic Hapludalfs. Anryong series occur on mountain foot slope positions in colluvial materials derived from acid and intermediate crystalline rocks. They are developed as Ultisols with clay mineral weathering, translocation of clays to accumulate in an argillic horizon, and leaching of base-forming cations from the profile for relatively long periods under humid and temperate climates in Korea.

• Journal of Korean Society of Forest Science
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• v.86 no.2
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• pp.186-199
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• 1997

The soils derived from granite gneiss occupy almost one third of the land area in Korea. The soils under forest vegetation, formed on granite gneiss, in Sun chon-shi, Chollanam-do in southern part of Korea, were studied to evaluate the weathering and the transformation of primary minerals into secondary minerals, clay minerals. The studied soils contained large amounts of ferromagnesian minerals, weathered biotites and were well weathered, strongly acid and low in organic matters and in ration exchange capacity. The clay contents in the Bt horizon were almost two times higher than those in the C horizon. The O horizon had a thin layer which consisted of a little decomposed plant components with a granic fabric and high porosity, and showed the micromorphological characteristics of moder humus. The related distribution pattern of the E horizon were enaulic and large amounts of silts and small amounts of sand grains were another characteristics of the E horizon. The most striking micromorphological features were multilaminated clay coating and infillings in the voids in the Bt and C horizons, and generally limpid ferriargillans ejected from the biotites and imparted red color to the soils in the Bt horizon. High clay contents in the Bt horizon was not only due to clay translocation, but also due to intensive in situ mineral weathering in this horizon. The most significant pedogenic process, revealed by the petrographic microscope and SEM, was the formation of iron oxides from biotites, the formation of tubular halloysites and the weathering models of biotites; wedge weathering and layer weathering. The thick coating on the weathering biotites showed the characteristics of the weathering process and the synthetic hematites were revealed in clays by TEM. Total chemical analysis of clays revealed extensive loss of Ca, and Na and the concentration of Fe and Al. Mineralogical studies of clays by XRD showed that micas were almost completely weathered to kaolinite, vermiculite-kaolinite intergrade, hematite, gibbsite, while halloysites from other primary minerals. Some dioctahedral mica appeared to be resistant in the soils. Parent rock of the soils contained a considerable amounts of biotites and this forest soils showed especially a dominant characteristics of biotite weathering.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.1
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• pp.45-52
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• 1985

Phytotron and field experiments were conducted to determine the influence of morphological development and environmental temperature on synthesis, translocation and accumulation behaviour of Fructosan, Monoand Disaccharose in corn cv. Blizzard and fodder sorghum cv. Sioux and Pioneer 931 at Munich technical university. Sorghum and maize plants were grown for 42 days at 4 temperature regimes (30/25, 25/20, 28/18 and 28/8 dog C) and mid-summer sunlight over 13-h days. The obtained results are summarized as follows: 1 Non-structural carbohydrates in maize and sorghum were accumulated mainly as Mono- and Disaccharose. The concentrations of Mono- and Disaccharose were increased markedly after differentiation of growing points and shown at early milk stage the highest contents with 27.8-29.1% and 16.8-20.4% for maizeand sorghums respectively. 2. Non-structural carbohydrates were accumulated mainly in stalk. However, during the late maturity the most of Mono- and Disaccharose were translocated into grain and reserved as starch. The increase of starch was associated with decrease of total non-structural carbohydrates. 3. Fructosan synthesis was not affected by morphological changes and environmental factors, which shows a value of 1.5-2.5% in whole stage of maize and sorghum. 4. Sorghum and maize plants were shown to have a great photosynthetic rates to high temperature. Reserved Mono- and Diaaccharose were, however, declined when temperature exceeded 30 dog C. Under cold stress at l8/8 deg C non-structural carbohydrates were not translocated and also were accumulated in leaves too much that cause to restrict of photosynthesis. 5. Net Energy Lactation (NEL) of sorghum and maize were directly associated with synthetic rates of non-structural carbohydrates, especially Mono- and Disaccharose. The highest values of NEL were found at physiological maturity stage with 6.6- 6.9 MJ and 5.7-6.0 MJ-NEL/kg for maize and sorghum respectively.

• Journal of Life Science
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• v.24 no.11
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• pp.1157-1167
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• 2014

In order to compare the anti-inflammatory effects of five selected cereal grains-proso millet, hwanggeumchal sorghum, foxtail millet, barnyard millet, and adlay-the inhibitory activities of 80% ethanol (EtOH) extracts obtained from the individual grains on lipopolysaccharide (LPS)-induced nitric oxide (NO) generation were investigated in RAW264.7 cells. The EtOH extract of barnyard millet (Echinochloa crus-galli var. frumentacea) grains exhibited more potent anti-inflammatory activity than that of the other grains. When the EtOH extract of barnyard millet grains was sequentially fractionated with n-hexane, methylene chloride (MC), ethyl acetate (EtOAc), and n-butanol, the majority of the anti-inflammatory activity was detected in the MC fraction, followed by the EtOAc fraction. Pretreatment with the MC fraction caused downregulation of the expression levels of iNOS- and COX-2-specific transcripts and proteins, as well as proinflammatory cytokine gene transcripts (IL-$1{\beta}$, IL-6, and TNF-${\alpha}$) in LPS-stimulated RAW264.7 cells. Additionally, the MC fraction could suppress not only the LPS-induced nuclear translocation of cytosolic NF-kB, but also the LPS-induced activation of MAPKs, such as ERK, JNK, and p38MAPK. Further analysis of the MC fraction by HPLC identified kaempferol, biochanin A, and formononetin as the major phenolic components. Both kaempferol and biochanin A, but not formononetin, could exert anti-inflammatory effect at the same concentrations as those of the MC fraction. Consequently, these results indicate that kaempferol and biochanin A are among the most effective anti-inflammatory phenolic components in barnyard millet grains. This finding suggests that barnyard millet grains and the MC extract enriched in kaempferol and biochanin A could be beneficial functional food sources that have an anti-inflammatory effect.

• Journal of Genetic Medicine
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• v.4 no.2
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• pp.133-141
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• 2007

Purpose : Cri-du-Chat syndrome (CdCs) is a rare but clinically recongnizable condition with an estimated incidence of 1:50,000 live births. The clinical characteristics of the syndrome include severe psychomotor and mental retardation, microcephaly, hypertelorism, hypotonia, and slow growth. Also the size of the chromosome 5p deletion ranges were known from the region 5p13 to the terminal region. In this study, we report the spectrum of 5p deletion in Korean 20 pts. with CdCs and genotype-phenotype associations in CdCs. Methods : In order to delineate genotype-phenotype correlation, molecular cytogenetic studies including GTG banding and clinical characterization were performed on Korean 20 pts with CdCs including parents. CGH array and Fluorescence in situ hybridization (FISH) analysis were used to confirm a terminal deletion karyotype and map more precisely the location of the deletion breakpoint. Results : Molecular analysis of the spectrum of 5p deletion revealed 9 pts (45%) with a del (5)(p14), 7 pts. (35%) a del (5)(p13), 3 pts. (15%) a del (5)(p15.1) and 1 pt. (5%) a del (5)(p15.2) in 20 pts with CdCs. 4(20%)pts were identified to have additional chromosome abnormalites of deficiency and duplication involving chromosomes of 6, 8, 18, & 22. Parental study identified 3 familial case (2 paternal and 1 maternal origin) showing parents being a balanced translocation carrier. And the comparison study of the deletion break points among these 20 pts. with their phenotype has showed the varying clinical pheno-types in the CdCs critical region. Conclusion : The characterization of 5p deletion including parental study may help to delineate the genotypephenotype correlation in CdCs. Also these molecular cytogenetic analyses will be able to offer better information for accurate genetic diagnosis in CdCs and further make possible useful genetic counseling in pts. and family.

• Childhood Kidney Diseases
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• v.8 no.2
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• pp.138-148
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• 2004

Purpose: Regardless of the underlying diseases, the proteinuric condition demonstrates ultrastructural changes in podocytes with retraction and effacement of the highly specialized interdigitating foot processes. We examined the molecular basis for this alteration of the podocyte phenotypes, including quantitative and distributional changes of ZO-1 protein as a candidate contributing to the pathogenic changes in the barrier to protein filtration. Methods: To investigate whether high glucose and advanced glycosylation endproduct(AGE) induce podocyte cytoskeletal changes, we cultured rat GEpC under 1) normal glucose(5 mM=control) or 2) high glucose(30 mM) or 3) AGE-added or 4) high glucose plus AGE-added conditions. The distribution of ZO-1 was observed by confocal microscope and the change of ZO-1 expression was measured by Western blotting and RT-PCR. Results: By confocal microscopy, we observed that ZO-1 moves from peripheral cytoplasm to inner actin filaments complexes in both AGE-added and high glucose condition. In Western blotting, high glucose or AGE-added condition decreased the ZO-1 protein expression by 11.1%(P>0.05) and 2.3%(P>0.05), respectively compared to the normal glucose condition. High glucose plus AGE-added condition further decreased ZO-1 protein expression to statistically significant level(12%, P<0.05). No significant change was seen in the osmotic control. In RT-PCR, high glucose plus AGE-added condition significantly decreased the expression of ZO-1 mRNA by 12% compared to normal glucose condition. Conclusion: We suggest that both high glucose and AGE-added condition induce the cytoplasmic translocation and suppresses the production of ZO-1 at transcriptional level and these changes may explain the functional changes of podocytes in diabetic conditions.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.57-62
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• 2006

Several methods for determining the response of corn to glyphosate were investigated to provide a fast and reliable method for identifying glyphosate-resistant corn in vivo. Two bioassays were developed. One assay is named 'whole plant / leaf growth assay', in which the herbicide glyphosate is applied on the upper part of 3rd leaf and the growth of herbicide-untreated 4th leaf is measured at 3 day after treatment. in this assay, the leaf growth of conventional corn was inhibited in a dose dependent from 50 to $1600{\mu}g/mL$ of glyphosate and growth inhibition at $1600{\mu}g/mL$ was 55% of untreated control. The assay has the potential to be used especially in the case that the primary cause of glyphosate resistance is related with a reduction of the herbicide translocation. Another assay is named 'leaf segment / shikimate accumulation assay', in which the four excised leaf segments ($4{\times}4mm$) are placed in each well of a 48-well microtiter plate containing $200{\mu}L$ test solution and the amount of shikimate is determined after incubation for 24 h in continuous light at $25^{\circ}C$. In this assay, 0.33% sucrose added to basic test solution enhanced a shikimate accumulation by 3 to 4 times and the shikimate accumulation was linearly occurred from 2 to $8{\mu}g/mL$ of glyphosate, showing an improved response to the method described by Shaner et al. (2005). The leaf segment / shikimate accumulation assay is simple and robust and has the potential to be used as a high throughput assay in the case that the primary cause of glyphosate resistance is related with EPSPS, target site of the herbicide. Taken together, these two assays would be highly useful to initially select the lines obtained after transformation, to investigate the migration of glyphosate-resistant gene into other weeds and to detect a weedy glyphosate-resistant corn in field.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.4
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• pp.389-398
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• 1984

The effect of N forms ($NO_3{^-}-N$, $NH_4{^+}-N$) and concentrations (4, 8, $16meq/{\ell}$) which were changed at head formation stage on the tip-burn incidence of chinese cabbage was studied under the three levels (0, 8, 16 meq/l) of Ca. All of the plants grown on $NH_4{^+}-N$ showed the symptoms of tip-burn and low yields regardless of Ca levels. Roots of plants grown on $NH_4{^+}-N$ were severely damaged. The pH of the leachate from $NH_4{^+}-N$ pot was decreased to below pH 5. Plants which had been grown on $NH_4{^+}-N$ before the head formation stage, but changed to $NO_3{^-}-N$ were recovered from abnormal growth. But, the reverse showed the tip-burn symptoms. $NH_4{^+}-N$ treatments increased the T-N contents, but lowered K and Ca contents of inner leafblades. Icreased applications of Ca did not affect the T-N and K contents of the inner leafblades. $NH_4{^+}-N$ suppressed Ca translocation into the inner leaves from outer leaves after the head formation stage, but $NO_3{^-}-N$ accelarated it. Ca contents were much lower in leaves showing tip-burn symptoms than in healthy leaves. Internal rot which is a tip-burn symptom occuring after head formation were noted in plants applied with high concentration of $NO_3{^-}-N$ both before and after the head formation stage. Ca contents correlated well with the rates of Ca application, but there was no correlation between ca conents and internal rot incidence. Chinese cabbage tip-burn is not caused by Ca deficiency, as is commonly believed, but rather by the water stress (95% water contents in $NO_3{^-}$-fed plants, 91% in $NH_4{^+}$-fed plants) resulting from root damage caused by ammonium toxicity. Internal rot is also caused by excess applications of nitrogen, and is unaffected by calcium levels.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.224-229
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• 2010

This study was conducted to reclassify Cheongpung series based on the second edition of Soil Taxonomy, and to dicuss the formation of Cheongpung series distributed on the diluvial terrace. Morphological properties of typifying pedon of Cheongpung series were investigated, and physico-chemical properties were analyzed according to Soil survey laboratory methods manual. The typifying pedon of Cheongpung series has red (2.5YR 4/6) silty clay loam Ap horizon (0-18 cm), red (2.5YR 4/8) clay BAt horizon (18-35 cm), red (2.5YR 4/2) cobbly clay Bt1 horizon (35-65 cm), and red (2.5YR4/6) cobbly clay Bt2 horizon (more than 65 cm). The typifying pedon has an argillic horizon from adepth of 18 to more than 65 cm, and a base saturation (sum of cations) of less than 35% at 125 cm below the upper boundary of the argillic horizon. It can be classified as Ultisol, not as Alfisol. It has udic soil moisture regime, and can be classified as Udult. Also that meets the requirements of Typic Hapludults. It has 35% or more clay at the particle-size control section, and have mesic soil temperature regime. Therefore Cheongpung series can be classified as fine, mesic family of Typic Hapludults, not as fine, mesic family of Ultic Hapludalfs. Cheongpung series occur on moderately elevated diluvial terrace which have relatively stable geomorphic surface. They are developed as Ultisols with clay mineral weathering, translocation of clays to accumulate in an argillic horizon, and leaching of base-forming cations from the profile for relatively long periods under humid, and temperate climates in Korea.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.5
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• pp.500-506
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• 2008

Enhanced phytoremediation with EDTA or PSM(Phosphate solubilizing microorganism) was studied using green foxtail (Setaria viridis) in columns packed with 1,200 mgPb/kg contaminated soil to investigate the effects of EDTA or PSM on the plant uptake and vertical migration of Pb. EDTA, equimolar amount of total Pb in the column soil, was administered in two methods: the one was treated with 1/6 aliquots of the equimolar EDTA every week for 6 weeks and the other was treated with single dose of the equimolar EDTA before 14 days of harvest. The results showed that higher concentrations of Pb accumulated in the biomass of green fowtail after the chemical or biological treatment. The plant-root Pb concentration in PSM treatment(M), EDTA aliquot treatment(ES), and single dose treatment(E) was 2.6, 3.0, and 3.3 times higher, respectively, than that in the plant-root of control(164.7 mg/kg). The plant-stem Pb concentration in the M, ES and E treatment was 27, 37, and 40 times higher than that in the stem of control(8.1 mg/kg). The translocation factor, the ratio of shoot/root Pb concentration, was 0.6 in the two EDTA treatment, 0.5 in the M treatment, and 0.05 in the control, respectively. The largest amount of Pb was phyto-extracted in the E treatment whereas vertical migration of EDTA was significant in the ES treatment. This result showed that a single large dose of EDTA before harvest serves better for enhanced phytoremediation of Pb. Although, treatment with PSM showed less Pb phytoextraction by the plant but enhanced both the growth of plants in the column and microbial dehydrogenase activity in the soils. Therefore, enhanced phytoextraction of Pb with PSM treatment can be an alternative option for EDTA treatment, which is toxic to plants and soil ecosystem.