• 생명과학회지
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• 제23권4호
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• pp.595-601
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• 2013

생물들에서 생명의 본질적 기능을 수행하는 단백질들의 종류와 보존성을 밝히기 위해 COG (Clusters of Orthologous Groups of proteins) 알고리즘을 이용하였다. 66종의 미생물에서 보존적인 63개의 ortholog 그룹들은 진핵생물 7종에서 104개의 ortholog들로 확산되었으며, 7종 모두의 핵에 보존적인 KOG (euKaryotic Orthologous Group)은 71개였다. 71개 중 단백질 합성에 관여하는 유전자들이 총 54개로 생명현상에서의 단백질의 중요성을 확인할 수 있었다. Distance value로 보존적 유전자가 생물종 사이에 나타내는 유전자 변이의 정도를 파악하니 'Translation initiation factor'인 KOG3403과 KOG3271 그리고 'Prolyl-tRNA synthetase' (KOG4163) 등이 높은 보존성을 보였다. 보존적 KOG들의 평균과 분산으로 유전체 분석을 수행하여 꼬마선충이 KOG 평균사이의 편차가 제일 커 유전자의 변이가 다양한 것을 알 수 있었다. 본 연구결과는 기초연구와 항생제 개발 등에 이용될 수 있을 것이다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2021년도 학술발표회
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• pp.153-153
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• 2021

Storm Storm event is one of major issues in South Korea due to devastating damage at its landfall. A series of statistical study on the historical typhoon records consistently insist that the typhoon translation speed (TS) is on slowdown trend annually, and thus provides an urgent topic in assessing the extreme storm surge under future climate change. Even though TS has been regarded as a principal contributor in storm surge dynamics, only a few studies have considered its impact on the storm surge. The landfall angle (LA), another key physical factor of storm surge also needs to be further investigated along with TS. This study aims to elucidate the interaction mechanism among TS, LA, coastal geometry, and storm surge synthetically by performing a series of simulations on the idealized geometries using Delft3D FM. In the simulation, various typhoons are set up according to different combinations of TS and LA, while their trajectories are assumed to be straight with the constant wind speed and the central pressure. Then, typhoons are subjected to make landfall over a set of idealized geometries that have different depth profiles and layouts (i.e., open coasts or bays). The simulation results show that: (i) For the open coasts, the maximum surge height (MSH) increases with increasing TS. (ii) For the constant bed level, a typhoon normal to the coastline resulted in peak MSH due to the lowest effect of the coastal wave. (iii) For the continental shelf with different widths, the slow-moving typhoon will generate the peak MSH around a small LA as the shelf width becomes narrow. (iv) For the bay, MSH enlarges with the ratio of L/E (the length of main-bay axis /gate size) dropping, while the greatest MSH is at L/E=1. These findings suggest that a fast-moving typhoon perpendicular to the coastline over a broad continental shelf will likely generate the extreme storm surge hazard in the future, as well as the slow-moving typhoon will make an acute landfall over a narrow continental shelf.

• 한국자기공명학회논문지
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• 제21권3호
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• pp.109-113
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• 2017

The hepatitis C virus (HCV) internal ribosome entry site (IRES) is an RNA structure located in the 5'-UTR of the HCV RNA genome. The HCV IRES consists of four domains I, II, III, and IV, where domains II - IV are recognized by 40S ribosomal subunit and the domain III is bound to eukaryotic initiation factor 3 (eIF3) for translation initiation. Here, we have characterized the tertiary interaction between an L-/K- rich peptide and the HCV IRES domain IV. To probe the peptide binding interface in RNA, we synthesized $^{13}C$- and $^{15}N$-double labeled RNA and the binding site was identified by using the chemical shift perturbation (CSP) NMR methods. Our results showed that the peptide binds to the upper stem of the IRES domain IV, indicating that the tertiary interaction between the IRES domain IV and the peptide would disrupt the initiation of translation of HCV mRNA by blocking the start codon exposure. This study will provide an insight into the new peptide-based anti-viral drug design targeting HCV IRES RNA.

• BMB Reports
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• 제47권10호
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• pp.558-562
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• 2014

OASL1 is a member of the 2'-5'-oligoadenylate synthetase (OAS) family and promotes viral clearance by activating RNase L. OASL1 interacts with the 5'-untranslated region (UTR) of interferon regulatory factor 7 (Irf7) and inhibits its translation. To identify the secondary structure required for OASL1 binding, we examined the 5'-UTR of the Irf7 transcript using "selective 2'-hydroxyl acylation analyzed by primer extension" (SHAPE). SHAPE takes advantage of the selective acylation of residues in single-stranded regions by 1-methyl-7-nitroisatoic anhydride (1M7). We found five major acylation sites located in, or next to, predicted single-stranded regions of the Irf7 5'-UTR. These results demonstrate the involvement of the stem structure of the Irf7 5'-UTR in the regulation of Irf7 translation, mediated by OASL1.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2022년도 제65차 동계학술대회논문집 30권1호
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• pp.9-10
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• 2022

본 논문에서는 딥러닝을 활용하여 취업준비생들의 데이터에 의하여 취업 가능 여부와 그에 따른 유용한 정보들을 얻기 위한 시스템을 제안한다. 취업 가능성이 성공적으로 평가된다면 예비 사회인, 취업준비생, 대학생들이 미리 취업 준비가 어느 정도 이루어졌는지 본인의 위치를 평가할 수 있으며 강점과 약점을 파악할 수 있을 것이다. 본 연구를 위해 취업생 및 취업준비생 데이터를 포함하는 CSV파일을 생성하였고, 딥러닝을 활용하여 유용한 정보들을 추출해내는데 성공했다. 이를 통해 취업 가능성 예측 프로그램은 취업준비생들과 기업의 인사관리자들에게 커다란 이점을 제공할 수 있을 것으로 보인다. 더 나아가 이 프로그램은 기업 구성원들의 업무능력을 평가할 수 있는 프로그램으로도 활용할 수 있을 것으로 사료된다.

• 대한간호학회지
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• 제45권3호
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• pp.439-448
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• 2015

Purpose: The purpose of this study was to examine validity and reliability of Webel and colleagues'HIV Self-Management Scale when used with a Korean sample. Methods: The original 20-item HIV Self-Management Scale was translated into Korean using translation and back-translation. Nine HIV nurse experts tested content validity. Principal component analysis (PCA) and confirmatory factor analysis (CFA) of data from 203 patients was used to test construct validity. Concurrent validity was evaluated using correlation with patients'self-rating as a "mart patient"measured using a visual analogue scale. Internal consistency was tested by Cronbach' alpha coefficients. Results: All items were rated as having satisfactory content validity. Based on PCA and consideration of conceptual meaning, a three-factor solution was selected, explaining 48.76% of the variance. CFA demonstrated the adequacy of the three-domain structure of the construct HIV self-management: daily self-management health practices, social support and HIV self-management, and chronic nature of HIV self-management. Goodness-of-fit indices showed an acceptable fit overall with the full model (${\chi}^2/df_{(164)}=1.66$, RMSEA=0.06, SRMR=0.05, TLI=0.91, and CFI=0.92). The Korean version of the HIV Self-Management Scale (KHSMS) was significantly correlated with patients'self-rated smart patient (r=.41). The subscale Cronbach' alpha coefficients ranged from .78 to .81; alpha for the total scale was .89. Conclusion: The KHSMS provides a valid and reliable measure of self-management in Korean patients with HIV. Continued psychometric testing is recommended to provide further evidence of validity with this population.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4235-4240
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• 2012

Objectives: Family caregivers (FCs) are often the primary source of social and emotional support for cancer patients and play a major role in how well they manage their illness. The aim of this study was to create an Turkish version of the Quality of Life - Family Version (QOL-FV) and to evaluate its psychometric properties in a sample of FCs of cancer patients. Materials and Methods: This study was carried out with the FCs of 218 patients with cancer. Data were collected with a Demographic Questionnaire and the QOL-FV and The Multidimentional Scale of Perceived Social Support (MSPSS). The QOL-FV was developed by Ferrell and Grant and is composed of 4 subdimensions. Linguistic validity, translation, back translation, and content validity were tested with expert opinions. Test-retest reliability, and internal consistency reliability were assessed. Construct validity was tested by factor analysis and with the scale of the MSPSS. Results: The family caregivers were between the ages of 46-56 (32.6%), a great number of them being male (52.8%). The scale is made up of four subdimensions. The result of the test-retest analysis of this scale was calculated as r:0.86. As a result of the reliability analysis, six items were eliminated from the scale, factor analyses were fulfilled according to varimax transformation through the method of principal components. Four new subdimensions were restrustured at the end of the analysis. The scale of Cronbach ${\alpha}$ coefficient was calculated as 0.90. Concurrent validity showed low correlations with the MSPSS (r=0.29). Conclusions: The QOL-FV, adapted into Turkish, was found to have sufficient reliability and validity.

• 기본간호학회지
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• 제24권4호
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• pp.296-303
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• 2017

Purpose: The purpose of this study was to evaluate the reliability and validity of the Korean version of Diabetes Empowerment Measurement Scale Short Form (DES-SF) for use with diabetic patients in Korea. Methods: A cross-sectional descriptive study was used with 107 participants. Bilingual nursing professionals performed translation and reverse translation. Reliability and validity such as face validity, construct validity, and concurrent validity were conducted. To assess the concurrent validity, the correlation coefficients between the Korean version of the DES-SF and a concurrent scale (Diabetes Self-Efficacy Scale, DSES) were calculated. Results: In the explanatory factor analysis, a uni-dimension was proposed, the one factor accounted for 49.4% of total variances. The Korean version of the DES-SF correlated with a concurrent variable such DSES for reliability of the Korean version of the DES-SF, Cronbach's ${\alpha}=.85$. Conclusion: The findings show that the Korean version of the DES-SF is reliable and valid for evaluating empowerment in patients with diabetes in Korea.

• 한국균학회지
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• 제45권4호
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• pp.292-303
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• 2017

2016년 버섯 재배사 내 실내공기, 식물병반(동백나무의 잎, 줄딸기, 은방울꽃, 생강나무), 버섯 배양배지 재료, 오염된 가구 등에서 진균을 분리 동정하였다. 동정된 진균 중 18종이 국내 미기록임을 확인하였다. 동정된 진균은 버섯 재배사 내 실내공기에서 6종, 식물병반에서 6종, 버섯 배양배지 재료에서 5종, 오염된 가구에서 1종이 각각 분리되었다 이들 동정된 균주에 대한 형태학적 특성과 ITS rDNA, 18S rDNA, 28S rDNA, calmodulin gene, translation elongation factor gene, ${\beta}-tubulin$ gene 등의 염기서열에 기반한 분자계통학적 관계에 대하여 기술하였다.

• 식물병연구
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• 제26권1호
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• pp.48-52
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• 2020

2019년 7월 안동지역 노지 재배지에서 복숭아에 과실 썩음병 피해가 발생하였다. 피해 과실에서는 병이 진전됨에 따라, 썩음증상과 함께, 흰색과 자주색을 띄는 균사 및 포자가 관찰되었다. 병원균을 순수 분리한 뒤, 건전한 복숭아 과일에 접종하였을 때 동일한 과실 썩음 증상을 유도하였다. 분리된 병원균의 internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF) 그리고 β-tubulin (β-TUB) sequence 분석을 통해 Fusarium avenaceum으로 동정되었다. 따라서, 이 증상을 Fusarium avenaceum에 의한 "복숭아 과실 썩음병"으로 명명하고자 한다. 분리된 균주는 농촌진흥청 국립농업과학원 미생물 은행[Korean Agricultural Culture Collection(KACC)]에 기탁되었다(KACC accession number 48936).