This experiment was conducted to evaluate the nutrient loss and to assess the eutrophication into small stream by intensive rains in the steeped cornfield during cultivation. The crop cultivated was a soiling com (DW5969), and the experimental plots were divided into two parts that were 10 and 18% of slope degrees. The amount of T-N and T-P loss was calculated by analysis of surface run-off water quality, and was investigated the effect of eutrophication to small stream as a part of life cycle assessment (LCA) methodology application. For the surface run-off water quality, EC and T-N values were highest in first runoff event as compared to the other events and maintained the stage state with litter variations at every hour during the runoff period except for EC in the slope 18%. However, T-P concentration has been a transient stage after runoff event of July 27. Total surface run-off ratio was not significantly different with slope degrees, but amount of T-N and T-P losses at 18% of slope were high as $5.96kg\;ha^{-1}\;and\;0.65kg\;ha^{-1}$ as relative to 10% of slope degree, respectively. Furthermore, T-N losses from run-off water in the sloped cornfield 10 and 18% were approximately 9.8 and 12.5% of the N applied as fertilizer when the fertilizer applied at recommended rates after soil test, respectively. For the eutrophication impact to the small stream, it was shown that $PO_4$ equivalence and Eco-indicator value at 18% of slope degree were greater as much $6.11kg\;ha^{-1}$ and 0.81 as compared to the slope angle 10%, respectively. Therefore, it was appeared that each effect of nutrient losses, eutrophication and Eco-indicator value was enhanced according with higher slope degree.
Transactions of the Korean Society of Mechanical Engineers B
/
v.35
no.5
/
pp.473-480
/
2011
The proton exchange membrane (PEM) fuel cell system is critically dependent on the humidity, which should be properly maintained over the entire operating range. A membrane humidifier is used for the water management in the PEMFC because of the membrane humidifier's reliable performance and zero parasitic power loss. In the PEMFC system, the membrane humidifier is required to provide appropriate humidity for the design point of the fuel cell. Although the performance of the fuel cell depends on the performance of the humidifier, few studies have provided a systematic analysis of the humidifier. We carry out an experimental analysis of the membrane humidifier using a vapor condensation bottle. The dry air pressure, water flow temperature, and air flow rate were chosen as the operating parameters. The results show that the time constant for the dynamic response of the membrane humidifier is relatively short, but additional analysis should be carried out.
Chung, Suk-Jae;Ramanathan, Vikram;Brett, Claire M.;Giacomini, Kathleen M.
Journal of Pharmaceutical Investigation
/
v.25
no.3
/
pp.7-20
/
1995
Taurine, a ${\beta}-amino$ acid, plays an important role as a neuromodulator and is necessary for the normal development of the brain. Since de novo synthesis of taurine in the brain is minimal and in vivo studies suggest that taurine dose not cross the blood-brain barrier, we examined whether the choroid plexus, the blood-cerebrospinal fluid (CSF) barrier, plays a role in taurine transport in the central nervous system. The uptake of $[^3H]-taurine$ into ATP depleted choroid plexus from rabbit was substantially greater in the presence of an inwardly directed $Na^+$ gradient taurine accumulation was negligible. A transient in side-negative potential gradient enhanced the $Na^+-driven$ uptake of taurine into the tissue slices, suggesting that the transport process is electrogenic, $Na^+-driven$ taurine uptake was saturable with an estimated $V_{max}$ of $111\;{\pm}\;20.2\;nmole/g/15\;min$ and a $K_M\;of\;99.8{\pm}29.9\;{\mu}M$. The estimated coupling ratio of $Na^+$ and taurine was $1.80\;{\pm}\;0.122.$$Na^+-dependent$ taurine uptake was significantly inhibited by ${\beta}-amino$ acids, but not by ${\alpha}-amino$ acids, indicating that the transporter is selective for ${\beta}-amino$ acids. Since it is known that the physiological concentration of taurine in the CSF is lower than that in the plasma, the active transport system we characterized may face the brush border (i.e., CSF facing) side of the choroid plexus and actively transport taurine out of the CSF. Therefore, we examined in vivo elimination of taurine from the CSF in the rat to determine whether elimination kinetics of taurine from the CSF is consistent with the in vitro study. Using a stereotaxic device, cannulaes were placed into the lateral ventricle and the cisterna magna of the rat. Radio-labelled taurine and inulin (a marker of CSF flow) were injected into the lateral ventricle, and the concentrations of the labelled compounds in the CSF were monitored for upto 3 hrs in the cisterna magna. The apparent clearance of taurine from CSF was greater than the estimated CSF flow (p<0.005) indicating that there is a clearance process in addition to the CSF flow. Taurine distribution into the choroid plexus was at least 10 fold higher than that found in other brain areas (e. g., cerebellum, olfactory bulb and cortex). When unlabelled taurine was co-administered with radio-labelled taurine, the apparent clearance of taurine was reduced (p<0.0l), suggesting a saturable disposition of taurine from CSF. Distribution of taurine into the choroid plexus, cerebellum, olfactory bulb and cortex was similarly diminished, indicating that the saturable uptake of taurine into these tissues is responsible for the non-linear disposition. A pharmacokinetic model involving first order elimination and saturable distribution described these data adequately. The Michaelis-Menten rate constant estimated from in vivo elimination study is similar to that obtained in the in vitro uptake experiment. Collectively, our results demonstrate that taurine is transported in the choroid plexus via a $Na^+-dependent,saturable$ and apparently ${\beta}-amino$ acid selective mechanism. This process may be functionally relevant to taurine homeostasis in the brain.
Objective : The transverse hippocampal slice is one of the most commonly studied in vitro models of mammalian brain physiology. However, despite its broad usage, there has been no standardization of slice preparation techniques or recording condition. It is well known that variations in recording conditions can result in profound different effects to neuronal responses. Evoked field potentials, recorded extracellularly, were used to investigate the effects of variations in hippocampal slice preparation protocol on hypoxia responses of CA1 neurones. Material & Methods : Before hypoxic injury, hippocampal slices were incubated for 4 hours. During incubation period, the slices were placed in a incubation chamber($21^{\circ}C$) for recovery from preparation injury and then transferred to recording chamber($34^{\circ}C$) for more recovery and baseline electric recording with current stimulation(0.1Hz). Various time periods in incubation chamber and recording chamber were applied to each experimental group(group 1=60min : 180min, group 2=90min : 150min, group 3=180min : 60min, time in incubation chamber : time in recording chamber) before 10 min hypoxia produced by replacing 95% $O_2$+5% $CO_2$ mixed gas to 95% $N_2$+5% $CO_2$ gas. Calcium, Magnesium ions and several drugs effecting on glutamate receptor also were studied. Recoveries from hypoxic injury of hippocampal slices were estimated by percent recovery of population spike(PS). Statistic analysis of study were performed using paired t-test. Results : The percent recovery of PS after 10min hypoxia was considerably enhanced by increasing the period of current stimulation during incubation period before hypoxic injury. Temperature effect on the result of this experiment was also studied(group 4) but the result from this showed no statistic significance. Low magnesium ion concentration of artificial CSF(Mg-free aCSF) during incubation period enhanced the recovery of PS but low calcium (calcium-free) and high magnesium ion concentration(2mM) reduced it after hypoxic injury. L-glutamate($100{\mu}M$) and AP-5($50{\mu}M$) had no effect on the recovery of PS but CNQX($10{\mu}M$) in artificial CSF during incubation period markedly enhanced the recovery of PS. Co-treatment of AP-5($50{\mu}M$), CNQX($10{\mu}M$) and high magnesium concentration(2mM) enhanced recovery of PS in immediate following period of hypoxic injury but the effect of cotreatment after then decayed rapidly and lost statistic significance. Conclusions : Judging from above results, the condition of baseline recording is important in observing the recovery of population spike after hypoxia, and the time and the condition should be controled more strictly to obtain reliable results.
The effects of $Ca^{++}$ and its antagonists (verapamil and $La^{+++}$) upon the spontaneous contraction and the contracture induced by 60 mM K-Tyrode solution were studied in the isolated uterine muscle. Longitudinal muscle strips were prepared from the rat uteri at estrous stage. All experiments were performed in tris-buffered Tyrode solution which was aerated with 100% $O_2$ and kept at 35^{\circ}$. The results obtained were as follows: 1) In the uterine strips contracting spontaneously, both the amplitude of peak tension and the area of contraction curve increased dose-dependently in the range of $0.5${\sim}8$ mM $Ca^{++}$. The frequency of contraction increased as the concentration of $Ca^{++}$ increased up to 2 mM, but above this concentration the frequency decreased. In $Ca^{++}-free$ media, however, contraction did not develop. In the contracture induced by 60 mM K-Tyrode solution, the developed tension increased dose-dependently as the concentration of external $Ca^{++}$ increased to 8 mM. In the absence of external $Ca^{++}$ K-contracture appeared, but it was not sustained. 2) The spontaneous contraction of rat uterus was suppressed by verapamil in proportion to an increase of its concentration and totally abolished at the concentration of $3{\times}10^{-4}\;g/l$, but the spontaneous contraction re-appeared by addition of $Ca^{++}$. The amplitude of peak tension recovered completely but the recovery of frequency was incomplete. K-contracture decreased in a dose-dependent manner after the treatment with verapamil and totally disappeared at its concentration of $3{\times}10^{-4}\;g/l$. Even in this case contracture developed again by extra $Ca^{++}$. 3) The spontaneous contractile activity was inhibited by $La^{+++}$. At the concentration of $10^{-4}$M $La^{+++}$, fibrillation appeared. In the strip inhibited by $10^{-5}M\;La^{+++}$, contractility recovered completely by extra $Ca^{++}$ while in the $10^{-4}M\;La^{+++}$ treated preparation, the rhythmic spontaneous contraction did not develop even at the concentration of 16 mM $Ca^{++}$. After the initial transient depression of contracture tension by $10^{-3}M$ of $La^{+++}$, the strip stowed considerably large size of contracture, hardly influenced by external $Ca^{++}$ or verapamil. The results obtained in this experiment suggest that in the rat uterine muscle there would be some competitive actions between $Ca^{++}$ and its antagonists. It is speculated that $Ca^{++}$ plays an important role in the conduction of excitation, and $La^{+++}$ influences upon cellular $Ca^{++}$ mobilization and re-uptake process as well as transmembrane $Ca^{++}$ transport in a K-depolarized state.
Journal of the Korean Society for Aeronautical & Space Sciences
/
v.45
no.3
/
pp.163-172
/
2017
Porous insulation have been frequently used in a number of industries by minimizing thermal insulation space because of excellent performance of their thermal insulation. This paper devices an effective thermal conductivity prediction model. First of all, we perform literature survey on traditional effective thermal conductivity prediction models and compare each other model with heat transfer experimental results. Furthermore this research defines advanced effective thermal conductivity prediction models model based on heat transfer experimental results, the Zehner-Schlunder model. Finally we verify that the newly defined effective thermal conductivity prediction model has better performance prediction than other models. Finally, this research performs a transient heat transfer analysis of thermal protection system with a porous insulation using the finite element method and confirms validity of the effective thermal conductivity prediction model.
The confirmed integrity of nuclear fuel cladding materials is an important object during steady state and transient operations at nuclear power plant. In this context, the clad material yielding behavior is especially important because of pellet-clad gap expansion. During the steep power excursion, the in-pile irradiation behavior differences between uranium-dioxide fuel pellet and zircaloy clad induce the contact pressure between them. If this pressure reaches the zircaloy clad yield pressure, the zircaloy clad will be plastically deformed. After the reactor power resumed to normal state, this plastic permanent expansion of clad tube give rise to the pellet-clad gap expansion. In this paper, the simple mandrel expansion test method which utilizes thermal expansion difference between copper mandrel and zircaloy tube was adopted to simulate this phenomenon. That is, copper mandrel which has approximately three times of thermal expansion coefficient of zircaloy-4 (PWR fuel cladding material) were used in this experiment at the temperature range from 400C to 700C. The measured plastic expansion of zircaloy outer radius and derived mathematical relations give the yield pressure, yield stress of zircaloy-4 clad at the various clad wall temperatures, the activation energy of zircaloy tube yielding, and pellet-clad gap expansion. The obtained results are in good agreement with previous experimental results. The mathematical analysis and simple test method prove to be a reliable and simple technique to assess the yielding behavior and gap expansion measurement between zircaloy-4 tube and uranium-dioxide fuel pellet under biaxial stress conditions.
The experiment was carried out to investigate the absorption profile of blood glucose in mice administered to silkworm powder MeOH extract. The mice was injected to oral load of maltose, sucrose and lactose(2 g/kg) and silkworm powder MeOH extract at the same time. After injecting the sample, blood glucose concentration was measured at 0, 30, 60, 120, 180 and 240 minutes. Blood glucose lowering effect of silkworm powder for loading maltose was 69% in postprandial 30 minutes. After the postprandial 60 minutes, the glucose was absorbed slowly. Total amount of blood glucose absorption in mice administered to maltose were 560.7 mg/dl during 240 minutes. That of silkworm powder MeOH extract marked 534.7 mg/dl. Total amount of blood glucose from oral loading sucrose reached to 508.9 mg/dl. That of loading silkworm powder MeOH extract were 468.8. But, silkworm powder was not inhibited lactose absorption. As a above results, silkworm powder inhibits the transient rising of blood glucose after postprandial 30 minuts through inhibition of ${\alpha}$-glucosidases. In case of starvation silkworm powder don't promote the hypoglycemia. In addition, silkworm powder induces the delay absorption of glucose without loss of it.
Journal of the Korean Institute of Telematics and Electronics T
/
v.35T
no.1
/
pp.90-98
/
1998
This paper is to describe the improved vector control which can control the induction motor robustly in low speed. When the induction motor is drived with low speed, below 10 percent of the rated speed, an algorithm which can compensate the error of unit vector angle generated by the harmonics is proposed. Another algorithm which can be tuned to the rotor time constant so that nay be robust to the rotor parameter change in low speed and transient state was proposed. The ripple of flux and torque was reduced by the proposed vector control and then the stable output characteristics was obtained in low speed. When the input and output is sinusoidal, the proposed vector control, the direct vector control and the indirect vector control were analyzed and compared in the low speed characteristics. And each control characteristics is compared and analyzed in state of containing harmonics. The estimation and tunning performance of rotor time constant is confirmed with simulation. The whole control system is implemented by real hardware and experimented to compare the proposed vector control with the direct vector control. As a result of the experiment with two control methods in low speed, the torque ripple of the proposed vector control is improved by 45 percent than the direct vector control. And it is confirmed that the flux current ripple is reduced in 0.2 p.u. and torque current ripple is reduced in 0.6 p.u. It is confirmed that the rotor time constant by the estimation and the tunning algorithm is tunned by the real rotor time constant. Finally, it was confirmed that the validity and robustness for the proposed vector control in low speed existed.
Objectives : This study was to evaluate the single dose toxicity of Persicae Semen (PS) in ICR mice. Methods : Aqueous extracts of PS (Yield = 18.60%) were administered as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) guidelines (2009-116, 2009). Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 12 principle organs were examined. Results : Amygdalin contents in PS aqueous extracts were detected as $32.50{\pm}5.96{\mu}g/ml$. We could not find any PS extracts treatment related mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations up to 2,000 mg/kg in both female and male mice, except for transient and slight loss of locomotion detected in female and male mice treated with 2,000 mg/kg. In addition, pharmacological immunomodulatory effects related findings were also demonstrated in 2,000mg/kg treated female and male mice as hypertrophy and hyperplasia of lymphoid cells in the submandibular lymph nodes. Conclusions : Based on the results of this experiment, the approximate lethal dose (ALD) of PS extracts after single oral treatment in female and male mice were considered above 2,000 mg/kg, respectively. It should be carefully used in clinics because the possibilities of respiratory or neurological disorders were observed when administered over 2,000 mg/kg of PS extract related to amygdalin.
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