• KSBB Journal
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• 제31권1호
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• pp.33-39
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• 2016

Developing the method for the selection of animal cell line producing therapeutic monoclonal antibody (mAb) is invaluable as its market is rapidly growing. Although the quality of produced mAb is as important as quantity, however there is no method developed for the selective screening of cell lines on the basis of both quantity and quality. From recent reports, the ratio of light and heavy chain mRNAs of mAb in the cell is a key parameter for the indication of product quality. Therefore, it is obvious that developing the novel method that can detect both light and heavy chain mRNAs in single live cell will provide unprecedented opportunities in bio-industry. Here, we have constructed oligonucleotide probes, molecular beacons for the detection of light or heavy chain mRNAs, respectively, in the live cells producing mAbs. Both beacons showed increased fluorescent intensity after transient transfection of plasmid expressing mAbs analyzed by fluorometer. Flow cytometric analysis clearly demonstrated that both molecular beacons can simultaneously detect the expression of light and heavy chain mRNAs of mAb in the same cell. The technique described in the thesis provides the new direction and concept for developing the method for the smart selection of cell lines producing recombinant proteins including therapeutic mAbs.

• Nuclear Engineering and Technology
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• 제54권8호
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• pp.3085-3094
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• 2022

The design concept of a Small High-flux Multipurpose LBE(Lead Bismuth Eutectic) cooled Fast Reactor (SHMLFR) was proposed in the paper. The primary cooling system of the reactor is forced circulation, and the fuel element form is arc-plate loaded high enrichment MOX fuel. The core is cylindrical with a flux trap set in the center of the core, which can be used as an irradiation channel. According to the requirements of the core physical design, a series of physical design criteria and constraints were given, and the steady and transient parameters of the reactor were calculated and analyzed. Regarding the thermal and hydraulic phenomena of the reactor, a simplified model was used to conduct a preliminary analysis of the fuel plates at special positions, and the temperature field distribution of the fuel plate with the highest power density under different coolant flow rates was simulated. The results show that the various parameters of SHMLFR meet the requirements and design criteria of the physical design of the core and the thermal design of the reactor. This implies that the conceptual design of SHMLFR is feasible.

• 대한조선학회 특별논문집
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• 대한조선학회 2015년도 특별논문집
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• pp.50-55
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• 2015

Application of newly conceptualized Anti-Splash Devices designed for COT vent pipes were studied on a P/V valve located on the upper deck of an oil carrier vessel. Anti-Splash devices are used in the shipbuilding industry in order to avoid oil overflow and spray accidents caused by excess pressure and vacuum condition in the cargo oil tanks. These conditions are caused by the transverse and longitudinal sloshing forces that arise from ship motion during sea voyages. The main issue with existing Anti-Splash device model is flux at the outlet of the Anti-Splash Device, and so, new conceptual models for the Anti-Splash device were developed and compared to existing Anti-Splash device model using CFD analysis. Transient analysis was used to capture the flow and velocity of each model and a comparative analysis was performed between old and new-concept models. This data was used to determine the optimal design parameters in order to develop an optimized Anti-Splash Device. A Factory acceptance test was performed on the new-concept models in order to verify the performance and efficiency against their design requirements and other criterion. The final step performed was to apply the optimized Anti-Splash Device models for COT vent pipes to an actual vessel and verify performance through a seawater cargo operation during a sea voyage as per the ship owner's request. The patent for the aforementioned device was obtained by the Korean Intellectual property Office dated Dec. 18th,2014.

• Nuclear Engineering and Technology
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• 제56권8호
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• pp.2974-2989
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• 2024

The one-dimensional Two-Fluid concept uses an area-average approach to simplify the time and phase-averaged Two-Fluid conservation equations, making it more suitable for addressing difficulties at an industrial scale. Nevertheless, the mathematical framework has inherent weaknesses due to the loss of details throughout the averaging procedures. This limitation makes the conventional model inappropriate for some flow regimes, where short-wavelength perturbations experience uncontrolled amplification, leading to solutions that need to be physically accurate. The critical factor in resolving this problem is the integration of closure relations. These relationships play a crucial function in reintroducing essential physical characteristics, thus correcting the loss that occurs during averaging and guaranteeing the stability of the model. To improve the accuracy of predictions, it is essential to assess the stability and grid dependence of one-dimensional formulations, which are particularly affected by closure relations and numerical schemes. The current research presented in the text focuses on improving the well-posedness of the TFM, specifically within the TRACE code, which is widely utilized for nuclear reactor safety assessments. Incorporating a bubble collision model in the momentum equations is demonstrated to enhance the TFM's resilience, especially in scenarios with high void fractions where conventional TFMs may face challenges. The analysis presents a linear stability analysis performed for the transient one-dimensional Two-Fluid Model of system code TRACE within the framework of vertically dispersed flows. The main emphasis is on evaluating the stability characteristics of the model while also acknowledging its susceptibility to closure relations and numerical techniques.

• 대기
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• 제18권1호
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• pp.55-70
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• 2008

The global time slice approach is a transient experiment using high resolution atmosphere-only model with boundary condition from the low resolution globally coupled ocean-atmosphere model. The present study employs this "time slice concept" using ECHAM4 atmosphere-only model at a horizontal resolution of T106 with the lower boundary forcing obtained from a lower-resolution (T42) greenhouse gas + aerosol forcing experiment performed using the ECHO-G/S (ECHAM4/HOPE-G) coupled model. In order to assess the impact of horizontal resolution on simulated East Asian summer monsoon climate, the differences in climate response between the time slice experiments of the present and that of IPCC SRES AR4 participating 21 models including coarser (T30) coupled model are compared. The higher resolution model from time slice experiment in the present climate show successful performance in simulating the northward migration and the location of the maximum rainfall during the rainy season over East Asia, although its rainfall amount was somewhat weak compared to the observation. Based on the present climate simulation, the possible change of East Asian summer monsoon rainfall in the future climate by the IPCC SRES A1B scenario, tends to be increased especially over the eastern part of Japan during July and September. The increase of the precipitation over this region seems to be related with the weakening of northwestern part of North Pacific High and the formation of anticyclonic flow over the south of Yangtze River in the future climate.

• 동력기계공학회지
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• 제12권1호
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• pp.13-19
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• 2008

In this study, a variable induction and exhaust system is applied to turbocharged diesel engine to improve the volumetric efficiency, especially, in a low and transient engine speed range where much of the pollutant matters are expelled out. The volumetric efficiency is known as one of the most important factor which affects significantly engine performance, fuel economy and further emission and noise level. As the torque increase with the engine speed up, the gas flow in an exhaust pipe become pulsating and then has an effect on boost up capacity of air charging into the cylinder and expelling capacity to atmosphere simultaneously. But at a low and idling speed, the pulsation effect was not so significant. Accordingly, resonator was employed to compensate their loss. The variable induction system consists of the secondary pipe, resonator, intercooler, and torque variance were examined with extended operating conditions. In the mean time, for interpretation and well understanding for the phenomena of wave action that arising during intake and exhaust process between turbocharger and variable intake system, the concept of the combined supercharging was introduced. Some of results are depicted which deal with a pressure history during valve events of induction process. Consequently, by the governing of these phase and amplitude of pulsating wave, it enables us to estimate and evaluate for the intake system performance and also, designing stage of the system layout.