• Journal of the Korea Organic Resources Recycling Association
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• v.21 no.2
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• pp.56-62
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• 2013

Organic wastes are not disposing object but renewable resources. One of those ways of rendering to resources, composting is environmentally friendly way as to aspect of recycling of resources. Regardless of ways, composting goes through the main processes which are fermentation process and curing process. In the study, mobile composting device was developed with the experiments which were performed to solve the problems of fermentation and curing process. Since reaction vessel is rotated, it does not cease in the middle of agitation because of foreign. In addition, it mixed as well. With maintaining uniform temperature of reaction vessel by controlling amount of air, fermentation and curing are easy to use. Its economic feasibility is better than the existing fermentation equipments. Furthermore, it benefits to reduce the personnel and materiel maintenance cost, and mass produce composting product which use organic waste. Especially, although it needs less space, it has effect to provide transferable composting device with available area to increase by contacting organic waste to air.

• Journal of Embryo Transfer
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• v.13 no.3
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• pp.213-217
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• 1998

The present study was undertaken to compare the superovulatory response to single subcutaneous (s.c.) injection of FSH dissolved in various solvents with standard multiple intramuscular (i.m.) injection. 1. Standing estrus was observed 100% in group 1 and 7 after the superovulation treatment. 2. Average numbers of total embryos and transferable embryos were significantly higher (p〈0.05) for group 1 and 7 (3.5, 2.9 and 3.8, 3.5) compared with another group. 3. Serum progesterone also was significantly higher(P〈0.05) in group 1 and 7 (6.19ng/ml, 7.54ng/m1) compared with another group. These data indicated that the single injection treatment using FSH diluted with PEG 30% was effective in superovulatory Korean cattle.

• Journal of Embryo Transfer
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• v.14 no.2
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• pp.113-119
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• 1999

This study was performed to improve the development of the in vitro fertilized bovine embryos by the condition of in vitro maturation. COCs were matured in TCM 199 supplemented with 0.1% PVA, 10ng/ml EGF, Hormones (5$\mu\textrm{g}$/ml FSH, 10 IU hCG, 1 $\mu\textrm{g}$/ml estradiol 17-$\beta$) or granulsa cell+Hormones atmosphere 39$^{\circ}C$, 5% CO2, 95% air for 24hrs. Matured oocytes were fertilized with frozen-thawed semen capacitated with 5mM caffein in BO medium for 20 hrs. IVF embryos were cultured in TCM 199 containing with hormones(same as matured medium), 10% FBS and co-culture with bovine oviduct epitherial cells. Maturation rates of COCs were showed 73.8%, 78.5%, 83.2% and 87.6% respectively, and were significant differences between PVA, EGF, and Hormones, GC+Hormones(p<0.05). The cleavage rates of IVF embryos were revealed 72.5%, 78.4%, 82.3% and 84.2% and showed same tendency as maturation rates(p<0.05). The blastocysts matured by above maturation condition and cultured for 7~10 days after fertilization had 34.4, 43.6, 52.3 and 59.3 cells had no differences among the treatments. These results suggest that high molecules as a substitutes of serum and growth factor may induce nuclear resumption of COCs but we need more study to produce transferable IVF blastocysts by use of that agents.

• Journal of Fisheries and Marine Sciences Education
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• v.10 no.2
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• pp.162-183
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• 1998

The fisheries resources control system in the Fisheries Act of Korea is introducing technical management method and input control method that controls fishing effort. Fishing effort control system of Korea aiming at realizing the maximum sustainable yield does not regulating the limitation of fishing quota and the limitation of fisheries object target fish. Therefore fishing operators who have fishing permit can use fishery resources without any restriction of fishing quota. But there are no rules that can controlling capacity of productivity of fishing by developing of fishing technic and fishing gear. For those reasons, productivity of fishing is superior to reproductivity of fisheries resources. Therefore, the Fisheries Act of Korea rearranges a legal basis for an introduction of fisheries resources management system by TAC, but the contents to be possible for a legal guarantee is not included and it is exceedingly defective as abstract and institutional devices. And that the affairs to be required for an enforcement of the said regime was placed in an administrative mandatory legislation and the danger to be degenerated is high in accordance with the bureaucratic self-righteous and/or the coercion of group's interest concerned and accordingly its substitute legislation system is keenly required. TAC system that is going to be introduced in our country is expected to enforce the Olympic fishing method and the individual quota method in parallel. This method is not certainly proper, because it occurs to overcapitalize and to compete fishing amounts between fishery operators. So as to prevent overcapitalization and fishing competition between fishery operators, and the exhaustion of coastal fisheries resources, individual transferable quota system should be introduced in Korean sea. Accordingly this thesis has attempted to constitute a view to improving problems of the traditional fisheries resources control system and introducing TAC fisheries resources control system.

• Korean Journal of Microbiology
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• v.26 no.3
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• pp.173-180
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• 1988

Chromosomal gene transferable hybrid plasmids, RP4::Mu cts and RP4::mini-Mu, were transferred by conjugation from E. coli to Pseudomonas strains. In order to use for recipient cells of RP4::Mu cts and RP4:: mini-Mu, plasmid-free Pseudomonas strains were characterized for their antobiotic resistance, aromatic hydrocarbon utility and degradation patterns of chlorinated herbicide. Transfer frequencies of RP4::mini-Mu exhibited about $10^{-2}$ to $10^{-4}$, while those of RP4::Mu cts exhibited very low value of $10^{-7}$ in recipients tested except Pseudomonas aeruginosa KU557. Existance of hybrid plasmids in Pseudomonas transconjugants were identified by their antibiotic resistance and agarose gel electrophoresis. In case of RP4::Mu cts transconjugants it was also confirmed by demonstrating that they were capable of releasing phage and forming plaques at $43^{\circ}C$. Plaque forming unit of the transconjugants was about $10^{5}$. It was shown by the stability test that RP4::Mu cts and RP4::mini-Mu in Pseudomonas were relatively stable.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.37
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• pp.277-327
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• 2008

This paper is to provide for an analysis on the trade adjustment assistance program and the Case study affected by international trade and FTA. The paper offers a legal study on analysis about Korea's Trade Adjustment Assistance Law for the Manufacturing Industries, USA's TAA. and Japan's law for Industry revival. In general, USA's TAA measures are defined as assistance actions for business enterprises, farmers and manufacturing industries workers. We exclude farmers and fishermen under Korea's Trade Adjustment Assistance Law for the Manufacturing Industries's business enterprises and workers, but we give them the systemic supports under korea's special assistance law for the farmers and fishermen and so on after FTA. Especially this focuses on the trade adjustment assistance case about both Korea's agriculture-farmer assistance from FTA and USA's TAA from workers in layoffs of trade-impacted communities. Korea has provided TAA to the sphere of agriculture after special legislation in Korea-Chile helping closed orchard, modernizing high quality producing facility for agriculture competition, producing superior fruit saplings, and constructing the distribution center in the producing district. But for US-Korea FTA and rapid increase of import by FTA expansion, we must prepare such indemnity systems for loss as cash benefit and wider aids for closed farm. The USA's TAA program targets manufacturing workers affected by international trade, who may have fewer transferable skills and face greater challenges to reemployment than other dislocated workers. A large majority sought some assistance from their one-stop center. Relatively small numbers chose to enroll in training, but those who did it often used this opportunity to chart a new career path. Finally, we learned the importance of education and discipline from USA cases. The fast and accurate information providing can raise efficiencies. The infrastructure can maximize the effect of TAA. The effective application would help us get over difficulties of TAA at hand.

• Journal of Embryo Transfer
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• v.12 no.1
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• pp.111-116
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• 1997

The present study was carried out to produce in vitro fertilized embryos with immature follicular oocytes collected by transvaginal aspiration from Holstein cows. A simple aspiration apparatus consists of two stainless steel tubes, an inner tube (needle holder; 1.2cmdiameter, 55cm long) and an outer tube (1.5cm diameter, 4Scm long), and a hand-operated vacuum pump was used. Under epidural anesthesia, the needle guide was passed into the vagina of the cow to a point next to the cervix. An ovary was placed against the wall of the vagina over the end of the aspiration needle by rectal manipulation. As the needlepassed into the ovary, an assistant was asked to apply vacuum(l00mrnHg) and the ovary was manipulated back and forth in all directions over the needle. When all sites of the ovary was aspirated, the needle was withdrawn and the needle guide was moved to the other side of ovary and the procedure was repeated. When the oocyte aspiration procedure was finished, collected fluid was transported to laboratory. Oocytes surrounded with at least 1 layer of cumulus cells were matured, fertilized and cultured in vitro. The results were as follows; Ninety seven oocytes were collected by transvaginal aspiration from seventeen Holstein cows(5.7 /head). The number of oocytes surrounded with at least 1 layer of cumulus cells were 60(61.9%). Following in vitro maturation, fertilization and culture, the cleavage and development rate to morula+blastocyst were 83.3% and 30.0%, respectively. From this study, transferable in vitro fertilized embryos could be produced with imma- ture follicular oocytes collected by transvaginal aspiration from Holstein cows using a simple aspiration apparatus.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1716-1723
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• 2017

The rapid dissemination of extended-spectrum ${\beta}$-lactamase (ESBL)-producing Escherichia coli has significantly contributed to public health hazard globally. A total of 281 E. coli strains recovered from pigs and chickens between 2009 and 2015 in South Korea were analyzed for ESBL production. ESBL phenotypes were recognized in 14 E. coli isolates; ten and three ESBL-producing isolates carried only $bla_{CTX-M}$ and $bla_{CMY}$ genes, respectively, and one isolate harbored both genes. The predominant CTX-M and CMY types were CTX-M-15 (n = 8) and CMY-2 (n = 3). We also detected ESBL-producing isolates harboring $bla_{CTX-M-65}$, $bla_{CTX-M-14}$, $bla_{CMY-6}$, $bla_{DHA-1}$, and $bla_{TEM-1}$ genes. All ESBL-producing isolates showed resistance to the extent of the fourth generation cephalosporins, along with multidrug resistance. CTX-M-15-producing isolates showed higher MIC values than CTX-M-14- and CTX-M-65-producing isolates. The $bla_{CTX-M}$ and $bla_{CMY}$ genes have the potential to be transferable. The spreading of $bla_{CMY}$ and $bla_{CTX-M}$ genes was arbitrated mainly via Frep and IncI1 plasmids. Our isolates showed clonal diversity in PFGE analysis. This is the first report of E. coli isolates carrying $bla_{CMY-6}$ in chicken from South Korea. The emergence of CMY-6 ESBLs in a population of poultry suggests that extensive screening with long-term surveillance is necessary to prevent the dissemination of ESBL from chicken to human.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.6
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• pp.1-9
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• 2020

Reservoir storage and water level information is essential for accurate drought monitoring and prediction. In particular, the agricultural drought has increased the risk of agricultural water shortages due to regional bias in reservoirs and water supply facilities, which are major water supply facilities for agricultural water. Therefore, it is important to evaluate the available water capacity of the reservoir, and it is necessary to determine the water surface area and water capacity. Remote sensing provides images of temporal water storage and level variations, and a combination of both measurement techniques can indicate a change in water volume. In areas of ungauged water volume, satellite remote sensing image acts as a powerful tool to measure changes in surface water level. The purpose of this study is to estimate of reservoir storage and level variations using satellite remote sensing image combined with hydrological statistical data and the Normalized Difference Water Index (NDWI). Water surface areas were estimated using the Sentinel-2 satellite images in Seosan, Chungcheongnam-do from 2016 to 2018. The remote sensing-based reservoir storage estimation algorithm from this study is general and transferable to applications for lakes and reservoirs. The data set can be used for improving the representation of water resources management for incorporating lakes into weather forecasting models and climate models, and hydrologic processes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.3
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• pp.1489-1494
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• 2013

Tsunami happens rarely enough to allow a false sense of security, but when they do occur, there may be just minutes or hours for people to reach a safe location. Natural disasters like tsunami are inevitable and it is almost impossible to fully recoup damages caused by the disasters. However, it is possible to minimize the potential risk by developing early warning strategies. GIS modelling with its geoprocessing and analysis capability can play a crucial role in efficient mitigation and management of disaster. This study aims at developing integrated spatial information system processing model supporting tsunami evacuation action planning using geo-information technology such as GIS. The integration process classified into four phases. And in each phase, required input data and GIS processes are decided. The main effort in minimizing casualties in tsunami disaster is to evacuate people from the hazard area before tsunami strikes by means of either horizontal or vertical evacuation. The study provides essential spatial information for local decision making related with people's evacuation in tsunami-prone areas based on a modeling approach transferable to other coastal areas.