• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.21-27
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• 2020

Somatic cell nuclear transfer derived embryonic stem cells (NT-ESCs) have significant advantages in various fields such as genetics, embryology, stem cell science, and regenerative medicine. However, the poor establishment of NT-ESCs hinders various research. Here, we applied fasudil, a Rho-associated kinase (ROCK) inhibitor, to develop somatic cell nuclear transfer (SCNT) embryos and establish NT-ESCs. In the study, MII oocytes were isolated from female B6D2F1 mice and performed SCNT with mouse embryonic fibroblasts (MEFs). The reconstructed NT-oocytes were activated artificially, and cultured to blastocysts in KSOM supplemented with 10 μM fasudil. Further, the blastocysts were seeded on inactivated MEFs in embryonic stem cell medium supplemented with 10 μM fasudil. A total of 26% of embryos formed into blastocysts in the fasudil treated group, while this ratio was 44% in the fasudil free control group. On the other hand, 30% of blastocysts were established NT-ESCs after exposure of fasudil, which was significantly higher than the control group (10%). The results suggest that fasudil reduced blastocyst development after SCNT due to inhibition of 2 cell cleavage while improved the establishment of NT-ESCs through the anti-apoptotic pathway.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.7-8
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.9-9
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• 2006

• Journal of the Optical Society of Korea
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• v.1 no.1
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• pp.5-9
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• 1997

Energy transfer direction in $Er^{3+}-Tm^{3+}$-codoped fluorozirconate glasses has been studied. For $Er^{3+}-Tm^{3+}$-codoped glasses, the dependence of the green emission intensity on the pump power (Pex) of 800 nm has ranged from (Pex)$^2$ to (Pex)$^3$. From this multistep absorption, a 1.48 $\mu m$ emission from the $^3F_4{\rightarrow}^3H_4$ transition on Tm$^{3+}$ ion has been found to transfer into $^4I_{13/2}$, $^4I_{9/2}$ and $^4S_{3/2}$ on $Er^{3+}$ ion. In case of the 1.06 $\mu m$ pumping, the emissin ratio of $^3H_4$ level in $Tm^{3+}$ to $^4I_{13/2}$ in $Er^{3+}$ showed that the amount of the energy transfer from $Tm^{3+}$ into $Er^{3+}$ increased with the increasing concentration of $Tm^{3+}$ ion. Our two kinds of pumping scheme suggest that the direction of dominant energy transfer between $Er^{3+}$ and $Tm^{3+}$ should be dependent on whether the $^3F_4$ level resonates in $Tm^{3+}$ the level or not.

• The Transactions of the Korean Institute of Power Electronics
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• v.24 no.4
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• pp.279-285
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• 2019

This study proposes an enhanced switching pattern that can improve energy transfer efficiency in an active cell-balancing circuit using a multiwinding transformer. This balancing circuit performs cell balancing by transferring energy stored in a specific cell with high energy to another cell containing low energy through a multiwinding transformer. The circuit operates in flyback and buck-boost modes in accordance with the energy transfer path. In the conventional flyback mode, the leakage inductance of the transformer and the stray inductance component of winding can transfer energy to an undesired path during the balancing operation. This case results in cell imbalance during the cell-balancing process, which reduces the energy transfer efficiency. An enhanced switching pattern that can effectively perform cell balancing by minimizing the amount of energy transferred to the nontarget cells due to the leakage inductance components in the flyback mode is proposed. Energy transfer efficiency and balancing speed can be significantly improved using the proposed switching pattern compared with that using the conventional switching pattern. The performance improvements are verified by experiments using a 1 W prototype cell-balancing circuit.

• Progress in Medical Physics
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• v.33 no.4
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• pp.80-87
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• 2022

Purpose: Proton therapy has different relative biological effectiveness (RBE) compared with X-ray treatment, which is the standard in radiation therapy, and the fixed RBE value of 1.1 is widely used. However, RBE depends on a charged particle's linear energy transfer (LET); therefore, measuring LET is important. We have developed a LET measurement method using the inefficiency characteristic of an EBT3 film on a proton beam's Bragg peak (BP) region. Methods: A Gafchromic EBT3 film was used to measure the proton beam LET. It measured the dose at a 10-cm pristine BP proton beam in water to determine the quenching factor of the EBT3 film as a reference beam condition. Monte Carlo (MC) calculations of dose-averaged LET (LET_d) were used to determine the quenching factor and validation. The dose-averaged LETs at the 12-, 16-, and 20-cm pristine BP proton beam in water were calculated with the quenching factor. Results: Using the passive scattering proton beam nozzle of the National Cancer Center in Korea, the LET_d was measured for each beam range. The quenching factor was determined to be 26.15 with 0.3% uncertainty under the reference beam condition. The dose-averaged LETs were measured for each test beam condition. Conclusions: We developed a method for measuring the proton beam LET using an EBT3 film. This study showed that the magnitude of the quenching effect can be estimated using only one beam range, and the quenching factor determined under the reference condition can be applied to any therapeutic proton beam range.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.105-105
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.106-106
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.54-55
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• 2006

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.84-85
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• 2006