• 한국미생물·생명공학회지
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• 제27권6호
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• pp.500-508
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• 1999

Several white-rot fungi collected from the mountains of Korea were evaluated for their ability to decolorize azo, polymeric, and reactive dyes. Strains CJ-105, CJ-212 and CJ-315, identified as Trametes sp., Pleurotus sp. and Fomes sp., respectively, showed higher potential for decolorization of those dyes in either solid or liquid media. For Trametes sp. CJ-105, 100ppm of Remazol Brilliant blue R and 500ppm of Acid Red 264 were completely decolorized after 2 days under liquid culture. The dominating ligninolytic enzyme existing in the culture broth was laccase (E.C. 1.10.3.2). Also, Pleurotus sp. CJ-212 and Fomes sp. CJ-315 showed similar patterns in decolorization of Remazol Brilliant Blue R and Acid Red 264. The extent of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic enzymes produced by each strain. In addition to that Trametes sp. CJ-105 was highly effective in degradation of polycyclic aromatic hydrocarbons and pentachlorophenol by the activity of the ligninolytic enzymes produced. In this study, we found that white-rot fungi, Trametes sp. CJ-105(KFCC 10941), Pleurotus sp. CJ-212(KFCC 10943) and Fomes sp. CJ-315(KFCC 10942), were effective in decolorizing a wide range of structurally different synthetic dyes, as well as some chemical compounds which are known to be hardly degradable.

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.477-483
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• 1999

For Trametes sp. CJ-105, a kind of white-rot fungi which was collected from the mountain of Korea and was proven to be effective in decolorizing a wide range of structurally different synthetic dyes, the optimum conditions for mycelial growth and laccase(E.C. 1.10.3.2) production were investigated. Among various carbon sources, glucose showed the highest potential for the mycelial growth and laccase production, the optimum concentration being 2% glucose. For the nitrogen source, asparagine was good for the mycelial growth, while ammonium tartrate for laccase production(optimum concentration: 0.04%). The addition of thiamine and biotin increased both th emycelial growth and laccase production. When 2,5-xylidine was added as an inducer after the first day of culture, the production of alccase was seven-times higher than that in the absence of the inducer. The optimum pH and temperature conditions for laccase production by Trametes sp. CJ-105 were pH 5.0 and $25^{\circ}C$, respectively. In the 5L fermentation, the production of laccase reached a maximum of 340U/ml at the time when the ammonium ion was being rapidly depleted.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.630-635
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• 1997

Decolorization of congo red, methyl orange, poly R478, remazol brilliant blue R and crystal violet by white-rot fungus Trametes sp. CJ-105, isolated in Korea, was investigated. Remazol blue and methyl orange were almost completely decolorized after 2 days of culture, but congo red, crystal violet and poly R478 were decolorized by about 80%, 40% and 30% after 10 days of culture, respectively. As a result of determination of cell mass and enzyme activity, it was shown that color removal efficiency was related to cell mass and enzyme activity, and also found that only laccase (E.C.1.10.3.2) activity was existed in the culture broth. The decolorization ratios of remazol blue in the concentrations of 100ppm to 3, 000 ppm were 85% and above after 2 days of culture. In this study, we found that white-rot fungus, Trametes sp. CJ-105, was effective in decolorizing a wide range of structurally different synthetic dyes.