• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2006년도 추계학술대회
• /
• pp.65-74
• /
• 2006

Modem drug discovery requires rapid pharmacokinetic evaluation of chemically diverse compounds for early candidate selection. This demands the development of analytical methods that offer high-throughput of samples. Naturally, liquid chromatography / tandem mass spectrometry (LC-MS/MS) is choice of the analytical method because of its superior sensitivity and selectivity. As a result of the short analysis time(typically 3-5min) by LC-MS/MS, sample preparation has become the rate- determining step in the whole analytical cycle. Consequently tremendous efforts are being made to speed up and automate this step. In a typical automated 96-well SPE(solid-phase extraction) procedure, plasma samples are transferred to the 96-well SPE plate, internal standard and aqueous buffer solutions are added and then vacuum is applied using the robotic liquid handling system. It takes only 20-90 min to process 96 samples by automated SPE and the analyst is physically occupied for only approximately 10 min. Recently, the ultra-high flow rate liquid chromatography (turbulent-flow chromatography)has sparked a huge interest for rapid and direct quantitation of drugs in plasma. There is no sample preparation except for sample aliquotting, internal standard addition and centrifugation. This type of analysis is achieved by using a small diameter column with a large particle size(30-5O ${\mu}$m) and a high flow rate, typically between 3-5 ml/min. Silica-based monolithic HPLC columns contain a novel chromatographic support in which the traditional particulate packing has been replaced with a single, continuous network (monolith) of pcrous silica. The main advantage of such a network is decreased backpressure due to macropores (2 ${\mu}$m) throughout the network. This allows high flow rates, and hence fast analyses that are unattainable with traditional particulate columns. The reduction of particle diameter in HPLC results in increased column efficiency. use of small particles (<2 urn), however, requires p.essu.es beyond the traditional 6,000 psi of conventional pumping devices. Instrumental development in recent years has resulted in pumping devices capable of handling the requirements of columns packed with small particles. The staggered parallel HPLC system consists of four fully independent binary HPLC pumps, a modified auto sampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The process of Bioanalytical Method Validation is required by the FDA to assess and verify the performance of a chronlatographic method prior to its application in sample analysis. The validation should address the selectivity, linearity, accuracy, precision and stability of the method. This presentation will provide all overview of the work required to accomplish a full validation and show how a chromatographic method is suitable for toxirokinetic sample analysis. A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method developed to quantitate drug levels in dog plasma will be used as an example of tile process.

• 한국콘텐츠학회논문지
• /
• 제8권12호
• /
• pp.10-19
• /
• 2008

본 논문에서는 문화재의 실측된 3D 스캔(scan) 데이터로부터 내부문양 및 실루엣(silhouette)을 효과적으로 추출할 수 있는 자동화된 실루엣 추출 방법을 제안한다. 제안한 방법에서는 먼저 3D 벡터 데이터의 에지리스트(edge list)를 생성하고, 시점에 따라 윤곽 에지(contour edge)와 주름 에지(crease edge)를 결정한다. 다음으로 주름 에지에서 인접면들의 벡터 변화량을 검사하여 표면 실루엣을 추출한 후, 최종적으로 문화재의 도면(blueprint) 생성을 위한 윤곽 실루엣과 표면 실루엣을 추출한다. 제안한 실루엣 추출 방법의 성능 평가를 위하여 전통 기와, 자동차 및 석탑 데이터를 이용한 실루엣 추출 실험을 진행하였다. 제안한 방법은 기존의 임계값을 이용한 실루엣 추출 방법에서 불필요한 에지까지도 실루엣을 형성하는 것을 보완하여, 잡음과 같은 의미 없는 에지들을 효과적으로 제거함으로써 더욱 선명하고 깨끗한 실루엣 및 내부 문양을 추출하였다.

• 수산경영론집
• /
• 제14권1호
• /
• pp.19-43
• /
• 1983

Mankind has made traditional use of various ocean resources in such several forms as fish, plants food, means of transportation, and military purpose, followed by the recent exploitation of offshore subsoil or sea-bed minerals, energies, and utilization of ocean space. These available ocean resources come from the marine natural environment which has a distinct feature in view of the relationship between human wants and their capacity to meet them. Though these socially basic resources however bring forth the so-called scarcity or differential rent, their communal nature of ownership dissipates free gifts of nature endowed to society as a whole. Thus to maximize these rents and social welfare thereof, rents should be secured and preserved through a well-defined arrangements of property-ownership as well as appropriate comparison of competing uses of marine resources, taking full cognizance of their irreversible adverse effects of a specific choice on the alternatives. Here I showed the sources of rent yielded from the multiple uses of navigation, fishery, mariculture, minerals, and recreation site, and also summarized the presently widely-known analytic tool to measure these rents with emphasis on due care of the telescopic faulty of the appraiser in charge, viewed from the communal point, Finally, as communal property is in strict sense owned by the public at large, notwithstanding the restricted communal ownership at government or local governments control, effectiveness of competition I expect should be kept while transferring claims of these resources from the legal owner to private enterprise as well as while extracting their rent by her. In particular, various national or social objectives look forward to tile maximization of social efficiency. Discretionary system in noncompetitive method thus, is exceptionally suggested because of probable suspicion from the public whether these resources are transferred as a give away or not in discretion. And these realized rent payments, I propose, should be wisely taken advantage of in advancement of scientific research in marine nature to bring an incremental rent therefrom successively.

• Transactions on Control, Automation and Systems Engineering
• /
• 제4권3호
• /
• pp.224-230
• /
• 2002

In this paper, a counting algorithm hybridized with an adaptive automatic thresholding method based on Otsu's method and the algorithm that elongates markers obtained by the well-known watershed algorithm is proposed to enhance the exactness of the microcell counting in microscopic images. The proposed counting algorithm can be stated as follows. The transformed full image captured by CCD camera set up at microscope is divided into cropped images of m$\times$n blocks with an appropriate size. The thresholding value of the cropped image is obtained by Otsu's method and the image is transformed into binary image. The microbial cell images below prespecified pixels are regarded as noise and are removed in tile binary image. The smoothing procedure is done by the area opening and the morphological filter. Watershed algorithm and the elongating marker algorithm are applied. By repeating the above stated procedure for m$\times$n blocks, the m$\times$n segmented images are obtained. A superposed image with the size of 640$\times$480 pixels as same as original image is obtained from the m$\times$n segmented block images. By labeling the superposed image, the counting result on the image of microbial cells is achieved. To prove the effectiveness of the proposed mettled in counting the microbial cell on the image, we used Acinetobacter sp., a kind of ammonia-oxidizing bacteria, and compared the proposed method with the global Otsu's method the traditional watershed algorithm based on global thresholding value and human visual method. The result counted by the proposed method shows more approximated result to the human visual counting method than the result counted by any other method.

• 보존과학회지
• /
• 제22권
• /
• pp.15-30
• /
• 2008

공주 단지리 3지구 출토 와관묘를 구성하는 기와와 고토양을 대상으로 재료과학적 연구방법을 적용하여 기와의 제작기법 및 원료의 산지추정을 통해 와관묘의 성격을 검토하였다. 기와는 모두 암키와로서 백제기와의 특징을 가진 연질 및 경질 기와이며 표면과 단면에 유상조직을 가지고 있다. 또한 기와의 광물학적 및 지구화학적 분석 결과, 태토는 주변토양을 이용하였으며, 제작과정에서는 토양의 거정질 석영 등을 제거하여 정제된 상태의 태토를 사용하였을 것으로 추정된다. 소성온도 추정결과, 일부 경질 기와는 $940{\sim}1,020^{\circ}C$에서, 연질 기와는 $700{\sim}900^{\circ}C$에서 소성한 것으로 판단된다. 기와들은 오랜 기간 매장환경의 영향을 받아 유기물 함량이 높고 인체조직의 필수 미량원소들이 토양에 비해 농집되어 있다. 따라서 이 기와들은 시신을 직접 매장하는 일차장으로서의 역할을 수행하였을 것으로 판단된다. 또한 건물지에 사용한 기와와 크기, 형식, 제작방법이 유사하여 당시 사용되던 암키와를 매장용으로 사용하였을 것으로 해석된다.

• 한국농촌건축학회논문집
• /
• 제15권4호
• /
• pp.35-42
• /
• 2013

The study aims to investigate general characteristics of Jae-sil from an architectural perspective by grasping location, deployment, structure, and plan composition based on existing inhabited conditions in Jeollabukdo. Results derived from the study are as follows. First, the location of Jae-sil is classified into mountainous and village types, and the two types showed a similar distribution. The village type showed the most distribution in the foot of a mountain in the rear of village, while the mountainous type is close to graveyard. The Jae-sil were mostly exposed to south, southeast, and southeast, which was not significantly different from residence. For deployment of the Jae-sil, a "二" shaped house, where main quarter and gated building are parallel located, can be common, indicating that additional attached building is less required. Second, for the platform out of structural components, coursed masonry was conducted by using natural rocks, and placed were a prop on the platform, first line on processed foot stone, and others on natural foot stone. For pillar, front line round columns, while others square columns. The binding type of the upper part of pillar is dominated by jangyeosujang and sorosujang. For wooden frame structure, 5ryangs were the most distributed, and out of these, 1goju 5ryang was the most generally used. For a roof part, it was composed with gambrel roof with single eave, and the roof was constructed by placing traditional Korean tile roof on the top. Third, for a size of Jae-sil out of plan composition, although the facade and the side of it were presented in a diverse manner, 4 facade rooms and 1.5 side rooms were the most frequently presented, and all Jae-sil were equipped with front ceremonial space. For spatial composition of Jae-sil, main floored hall is placed at the center, and each room is placed on the left and right side to secure space for ancestral rite for participants of it, and for a house with 5 rooms or more, it shows that main floored hall and rooms are weighted from side to side.

• 대한지리학회지
• /
• 제39권3호
• /
• pp.297-320
• /
• 2004

1904년 대한제국 정부에서 간행한 $\boxDr$경상남도가호안$\boxUl$은 총 31개군 가운데 11개군의 자료가 현전하는데, 이 자료는 당시 이 지방의 민가에 대한 상세한 정보를 담고 있다. 이 논문은 제1차자료인 가호안의 분석을 통하여 동ㆍ면ㆍ군별 가좌의 토지등급ㆍ가옥의 규모, 가좌와 가옥의 소유관계, 초ㆍ와가의 분포 등을 파악한 연구이다. 가호안의 분석결과 전체 가호의 약 20%는 사유지 또는 국공유지를 임대한 가좌에 지어졌다. 또한 가옥의 90%는 방 한칸 또는 두칸에 부엌이 딸린 집이었으므로 지역의 가옥규모는 2.75칸에 불과하였다. 다시 말하면 100년전 경상남도의 가옥규모는 조선시대의 이상 가옥형인 삼간초옥에도 못미쳤던 것이다. 이러한 열악한 주거조건 때문에 1인당 점유공간이 2-4평방미터에 불과하며 결과적으로 수용가능한 가족의 수도 4인 내외로 한정되었다.

• 한국축산식품학회지
• /
• 제21권4호
• /
• pp.300-306
• /
• 2001

본 연구는 국내시장에 수입 유통중인 수입산 및 한국산 닭고기의 가슴부위육에 대한 이화학적, 미생물학적 및 관능학적 품질특성을 비교 분석하여 이에 대한 기초적인 품질정보를 제공하고자 실시되었다. 태국산 닭고기의 가슴부위가 조단백질 함량이 가장 높게 나타났고(P<0.001), 반면 조지방과 수분의 함량은 가장 낮은 수준을 나타내었다. 수분함량이 가장 높게 나타난 국내 재래시장에서 구입한 닭고기 가슴부위육은 미생물분석 결과 총균수가 $10^{5}$으로 가장 높게 나타났고(P<0.001), 대장균의 수 또한 가장 높게 나타났다(P<0.001). 육색에서 가장 높은 L값을 나타낸 태국산 닭고기 가슴육은 pH에서는 가장 낮은 수준을 나타내었다(P<0.01). 조지방함량이 가장 높게 나타내었으며, VBN에서는 국내 A회사에서 생산된 닭고기 가슴육이 가장 높게 나타났고, P<0.05수준에서 유의적인 차이를 나타내었다. 신선육에 대한 척도묘사분석에서는 국내 재래시장에서 구입한 닭고기 가슴육의 경우 불쾌취와 육즙삼출율에서 가장 높은 점수를 얻었으며, 기호도에서 가장 낮게 나타났으나, 반면 조리육에 대한 척도묘사분서결과 가장 높은 기호도를 보였다.다.

• 건축역사연구
• /
• 제17권4호
• /
• pp.65-82
• /
• 2008

The wooden pagoda sites which have been confirmed in Baekjae's former territory so far have flattened surface of the earth or foundation pert made by digging up the earth. In particular, the latter is found more often in the pagoda sites of Baekjae, which is essential and absolutely necessary because of the characteristics of pagoda structure. The wooden pagoda sites with foundation part made by digging up the earth under the stylobate are found at Yongjeongli ruined temple site of Woongjin area, and at Neung-sa temple site, Wangheung-sa temple site, Geumgang-sa temple site, and Mireuk-sa temple site of Sabi period. They are also observed at Hwanglyong-sa nine-storied wooden pagoda of Shilla and at Biin five-storied stone pagoda of early Goryeo. They are important data improving that the construction technologies of Baekjae continued to be applied to build stone or wooden pagodas, transcending time and space. Recently, the site assumed as a wood pagoda site of Hanseong area was examined in Gyeongdang sect ion of Pungnap mud fortification. If this is proved to be a real wooden pagoda site, this digging-up construction technology of foundation part ann be concluded to be a traditional engineering technology of Baekjae which was frequently used from Hanseong period to Sabi period. On the other hand, this digging-up construction technology of foundation part has been found only at pagoda sites and main building sites of temple ruins, and it helps examine their symbolism.

• 한국수산과학회지
• /
• 제11권2호
• /
• pp.65-83
• /
• 1978

This study was attempted to evaluate the taste of broiled-dried sea mussel which is a traditional dried Sea food in Korea. The contents of such compounds as free amino acids, nucleotides and their related compounds, TMAO, betaine, and nonvolatile organic acids were analysed. From the results of analysis of nucleotides, the contents of AMP and ADP in broiled-dried Mytilus coruscus and Mytilus edulis appeared higher than other nucleotides and tended to increase slightly after cooking, drying, and storage which might be due to both their stability and the addition of degradation of nucleic acids during cooking. In the free amino acid composition of fresh samples abundant amino were taurine, glycine, serine, glutamic acid, alanine, arginine, threonine, tyrosine, lysine and valine in order. Such amino acids as histidine, leucine, methionine and isoleucine were poor and both proline and phenylalanine were merely trace. The free amino acid composition in the extract of Mytilus corucus and Mytilus edulis after broil-drying was not changed. These amino acids, taurine, glycine, feline, glutamic acid, alanine and arginine were abundant in dried samples as well as in the fresh. The total free amino acid was greatly reduced after cooking and drying ranging from $76.3\%$ to $79.7\%$ loss to that of tile fresh. The content of betaine shelved the same tendency as in total free amino acid while TMA slightly increased relating to TMAO decrease during broil-frying. Such nonvolatile organic acids as succinic, lactic, malic and fumaric acid were abundant in both fresh and broiled-dried samples whereas oxalic and pyruvic acid were poor. It is found that the taste compounds of broiled-dried Mytilus coruscus and Mytilus edulis were composed of amino acids as glycine, serine, alanine, glutamic acid, arginine, and betaine, TMAO, ADP, AMP, and organic acids such as succinic acid, lactic acid, malic acid and fumaric acid. No significant differences in the taste compounds between male and female as well as between Mytilus coruscus and Mytilus edulis was not observed.