• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.2
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• pp.103-107
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• 2003

Phosphorus (P) is a macronutrient playing important roles in many plant processes. Significant interest has been devoted to search and utilize genotypic variations in P use efficiency in rice but with little effort to understand its physiological and biochemical bases. In this study, we examined responses to P deprivation of some primary and secondary traits in 3-week-old seedlings of the three genotypes, Sobi-byeo (japonica), Dasan-byeo (japonica $\times$ indica) and Palawan (indica). In general, percent weight due to root was increased up to 26%, but amounts of root protein and proteins secreted from roots were decreased by 11 to 19% and 31 to 51 %, respectively, by 3 to 21 days of P deprivation in the three genotypes. Interestingly, however, responses of Palawan to short-term P deprivation were contrasting to those of Dasan-byeo and Sobi-byeo in seedling weight and contents of shoot protein, chlorophyll and anthocyanin. Seedling weight was not decreased, but shoot protein content was decreased in P-deprived seedlings of Palawan. Contents of chlorophyll in leaves and anthocynin in roots were increased in Dasan-byeo and Sobi-byeo, but decreased in Palawan. The results suggest that responses of protein and pigment synthesis to P deficiency are different in modem and traditional varieties and the difference may at least in part be due to the selection for high yield under highly fertilized conditions.

• Journal of Applied Biological Chemistry
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• v.60 no.3
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• pp.249-256
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• 2017

The Monascus azaphilone (MAz) pigment is a well-known food colorant that has yellow, orange and red components. The structures of the yellow and orange MAz differ by two hydride reductions, with yellow MAz being the reduced form. Orange MAz can be non-enzymatically converted to red MAz in the presence of amine derivatives. It was previously demonstrated that mppE and mppG are involved in the biosynthesis of yellow and orange MAz, respectively. However, ${\Delta}mppE$ and ${\Delta}mppG$ knockout mutants maintained residual production of yellow and orange MAz, respectively. In this study, we deleted the region encompassing mppD, mppE and mppG in M. purpureus and compared the phenotype of the resulting mutant (${\Delta}mppDEG$) with that of an mppD knockout mutant (${\Delta}mppD$). It was previously reported that the ${\Delta}mppD$ strain retained the ability to produce MAz but at approximately 10% of the level observed in the wildtype strain. A chemical analysis demonstrated that the ${\Delta}mppDEG$ strain was still capable of producing both yellow and orange MAz, suggesting the presence of minor MAz route(s) not involving mppE or mppG. Unexpectedly, the ${\Delta}mppDEG$ strain was observed to accumulate fast-eluting pigments in a reverse phase high-performance liquid chromatography analysis. A LC-MS analysis identified these pigments as ethanolamine derivatives of red MAz, which had been previously identified in an mppE knockout mutant that produces high amounts of orange MAz. Although the underlying mechanism is largely unknown, this study has yielded an M. purpureus strain that selectively accumulates red MAz.

• Conservation Science in Museum
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• v.13
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• pp.13-22
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• 2012

The patterns for Jeogui and Pyeseul (patterns of the queen's ceremonial robe worn and a belt ornament covering knees in the front) as owned by National Museum of Korea were made in the early 20th century and both of them were colored on paper. Through a non-destructive analysis on pigments and dyes used on both the patterns, it was attempted to identify the coloring materials. As for the analysis of the coloring pigments, the XRF was used. As a result of the pigment analysis on the pattern for Jeogui, it is presumed that lead white was used for obtaining the white color, ink stick for black color, orpiment for yellow color, cinnabar and red lead for red color, copper and arsenical or a mixture of copper carbonate and orpiment for green color whereas brass seems to have been used for obtaining other colors. The pigments used for obtaining the colors of white, black, yellow and green on the pattern for Pyeseul are the same as those used on the pattern for Jeogui whereas the red pigments used on each of the two patterns are seen to be different. Although it may be difficult to identify the kinds of blue pigments that were used on the patterns for Jeogui and Pyeseul, a mixture containing lead white is presumed to be used as blue pigment. Also, as a result of the UV-Vis spectroscopic analysis performed on the blue hanji (traditional Korean paper) of the pattern for Jeogui, it was presumed to have been dyed with indigo.

• Journal of Conservation Science
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• v.35 no.6
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• pp.600-611
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• 2019

Yellow to reddish brown soil is generally referred to as hwangto and is used in various industries in Korea. Despite the fact that it is used as an inorganic pigment in dancheong, limited studies have been conducted on the properties of pigments associated with soil and on the mineralogical characteristics of hwangto. This study examines how the pedological and mineralogical features of hwangto affect pigment properties. Results indicate that reddish and yellowish soils have differences in terms of soil texture, mineral composition, oil absorption and stability under light. Reddish soil is mostly found in clay regions, whereas Ulleungdo hwangto is found in loam regions. Yellowish soil is mostly present in the clay loam to loam zones. whereas Haenam hwangto exists in the sandy clay loam zone. As a result of a mineralogical analysis, reddish soil is classified into the feldspar group and clay soil. The major minerals in the yellowish soils are similar however these soils differ in terms of clay mineral compositions. results of the characteristics of pigments prepared by the traditional method revealed that the average particle size is in the range of 10-20 ㎛, reddish soil has an average of 20 ml/100 g higher oil absorption than yellowish soil. In addition, reddish soil is more susceptible to discoloration and deterioration under light than yellowish soil. This study confirms that the soil and mineral characteristics of hwangto affect the physical properties and stability of produced pigments. These result can be used as basic data in future studies natural inorganic pigments using hwangto.

• Journal of Conservation Science
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• v.31 no.4
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• pp.403-409
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• 2015

Malachite and Azurite are the typical copper system pigments which used the mural paintings since ancient times. The mural painting is at risk for damages of the painting layer by atmosphere gas because it is exposed at external environment. In this study, it did experiment about an effect to Malachite and Azurite by environmental pollution gas($NO_2$, $CO_2$, $SO_2$) then analysis and estimate about test for pieces using mural painting colored that two pigments. As a result, Malachite and Azurite were changed on $NO_2$ but not changed $CO_2$ and $SO_2$. Especially as the concentration of $NO_2$ is increased, exfoliation of the pigment layer weave remarkably formed pores on the pigment particles on SEM, the phenomenon to be pieces were observed together with smaller particles. In the case of Malachite that were exposed to $NO_2$ gas, new compounds(Rouaite : dicopper (nitrate(V) trihydroxide, $Cu_2(NO_3)(OH)_3$)) was appeared by XRD analysis. Therefore, there had been able to verify the fact that the cause exfoliation and discoloration phenomena accompanied by chemical changes for Malachite and Azurite.

• Journal of the Mineralogical Society of Korea
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• v.32 no.4
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• pp.259-271
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• 2019

We evaluated mineralogical, physical property and accelerated weather resistance of cinnabar, realgar and orpiment used as cultural heritages and traditional paintings. JB and JS are sample names of cinnabar and JH and UH are sample names of orpiment and Realgar, respectively. As a result of mineralogical property evaluation, Jinsa was identified cinnabar (HgS) and there was no difference in chemical composition according to the grade. UH and JH were confirmed realgar (AsS) and orpiment (As₂S₃), respectively. The polarization characteristics such as shape and color indicate that pigments using our test were natural mineral pigment observed by polarization microscope. Especially, in the case of cinnabar, it is not easy to distinguish between natural mineral pigment and synthetic pigment. But the results of polarization microscope said that cinnabar is natural occurring mineral which have authentic mineral particle and unevenly fracture. As a result of thermal analysis, JH has a higher glass transition temperature and heat stability than UH. After accelerated weather test, cinnabar, realgar and orpiment were striking result in color change because of light degradation. Red color of cinnabar turn into black and the color of realgar and orpiment became brighter than before. JB (meta-cinnabar) is more photosensitivity and faster becoming dark than JB (cinnabar). Finally light transforms realgar (red, As₄S₄) in arsenolite (As₂O₃).

• Journal of the Mineralogical Society of Korea
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• v.31 no.1
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• pp.33-46
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• 2018

Traditional inorganic pigments applied to dancheong, buddhist painting, and wall painting were produced from natural minerals which were later replaced by synthetic pigments, resulting in the loss of the recipe to prepare mineral pigments. This study examined the domestic occurrence and mineralogical characteristics of green and blue mineral pigments required for the conservation of cultural heritage. Cuprous green-blue mineral pigments were found as the weathering products of waste dumps and ores of abandoned Cu-Pb-Zn sulfide mines. Mineralogical analyses using X-ray diffraction and scanning electron microscopy identified diverse hydrous copper sulfate pigments of green (brochantite and devilline) and blue color (linarite, bechererite, and schulenbergite) with minor green pigments of antlerite and atacamite commonly associated with cerussite, smithsonite, anglesite, and cuprite. Noerok, a green silicate pigment, replaced the fractured basalt lava. Celadonite was responsible for the green color of Noerok, closely associated with opal in varying ratio. Glauconite, green silicate pigment, was identified in the Yellow Sea sediments. Malachite and azurite, the most important green and blue pigments of Korean cultural heritage, were not identified in this study.

• Korean Journal of Mineralogy and Petrology
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• v.33 no.4
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• pp.361-376
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• 2020

Atacamite and Verdigris were studied material scientific properties and durability that are used for traditional green pigment in traditional art painting work and Dancheong. As a result of ingrediant analysis, K-AA and K-VA identified Atacamite and Hoganite (or Verdigris) respectively. In order to find a factor of depressing the stability of pigment, we examined UV radiant exposure test, CO2/NO2 gas corrosion test and salt spray test. Salt spray test damaged both samples which were formed salt particle on the surface of the samples and it makes color disability. Furthermore, the results of gas corrosion test that both pigments change color enough to be perceived by the naked eyes showed that an air pollutant NO2 gas is also considered to be a major damage factor. In the case of K-VA, Hoganite that is main component of sample changes Tenorite with turn black after accelerated UV radiant exposure test. The consequences of the atmospheric environment effect test of the two pigments, K-VA showed relatetively weaker than K-AA.

• Economic and Environmental Geology
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• v.43 no.3
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• pp.269-278
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• 2010

We investigated characteristics of the coloring material of Dancheong pigments and hope that this study contributes the revival of traditional Dancheong pigments color. For this purpose, we collected Dancheong fragment samples that fell off naturally from old wooden buildings in Gwangju and Jeonnam and analyzed the natural coloring material by XRD and EDS-SEM analysis method. In white pigments of Dancheong fragments, it is confirmed that gypsum$(CaSO_{4}{\cdot}2H_{2}O)$, quartz$(SiO_{2})$, white lead$(PbCO_{3})$ and calcite$(CaCO_{3})$ which have been used for white pigments since ancient times and $TiO_{2}$ which is common used in modern times. In red pigments of Dancheong fragments, it is confirmed that hematite$(Fe_{2}O_{3})$ and red lead$(Pb_{3}O_{4})$, which have been used for red pigments since ancient times and C.I. pigment orange $13(C_{32}H_{24}C_{12}N_{8}O_{2})$ but there is no cinnabar(HgS) which has been used since B.C. 3000 in China. In yellow pigments of Dancheong fragments, it is confirmed that crocoite$(PbCrO_{4})$ and massicot(PbO). In blue pigments of Dancheong fragments, it is confirmed that sodalite$(Na_{4}BeAlSi_{4}O_{12}Cl)$ and nosean $(Na_{8}Al_{6}Si_{6}O_{24}SO_{4})$ as coloring material of blue pigment and C.I. pigments blue $29(Na_{7}Al_{6}Si_{6}O_{24}S_{3})$ which is used in modern times. In green pigments of Dancheong fragments, it is confirmed that calumetite$(Cu(OHCI)_{2}{\cdot}2H_{2}O)$, escolaite(Cr2O3), dichromium trioxide$(Cr_{2}O_{3})$, emerald green$(C_{2}H_{3}As_{3}Cu_{2}O_{8})$, and C.I. pigments green$(C_{32}H_{16}-XCl_{x}Cu_{8})$ which is used in modern time. In black pigments of Dancheong fragments, Chiness ink(carbon black) is confirmed.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.375-380
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• 2018

We have previously found that mycelia culture broth of eight kinds of traditional herbal extracts fermented with Phellinus linteus (previously named as 8-HsPLCB) not only inhibited melanin and tyrosinase activity, but also reduced the contents of melanogenesis-related proteins, including tyrosinase and microphthalmia-associated transcription factor, in 3-isobutyl-1-methylxanthine-stimulated B16F0 melanoma cells. For a further study, the effect of 8-HsPLCB against skin pigmentation in brown guinea pigs with ultraviolet B (UVB)-induced hyperpigmentation was investigated. 8-HsPLCB (3%) and arbutin (2%) as positive controls were applied topically twice daily for 4 weeks to the hyperpigmented areas. 8-HsPLCB showed skin-lightening effect as effective as arbutin, one of the most widely used in whitening cosmetics. Melanin index values as the degree of pigmentation showed a significant reduction week by week post 8-HsPLCB treatment and then substantially reduced by 4 weeks. The degree of depigmentation after 4 weeks of topical application with 8-HsPLCB was 32.2% as compared with before treatment (0 week). Moreover, using Fontana-Masson staining and hematoxylin-eosin staining, 8-HsPLCB reduced melanin pigmentation in the basal layer of the epidermis and epidermal thickness changes exposed to the UV-B irradiation as compared with non-treatment and vehicle treatment. The intensity of the skin-lightening effect of 8-HsPLCB was similar to arbutin. These results suggest that the skin-lightening effect of 8-HsPLCB might be resulted from inhibition of melanin synthesis by tyrosinase in melanocytes. To conclude, 8-HsPLCB treatment showed reduction of the melanin pigment and histological changes induced by UV irradiation in brown guinea pigs.