In an effort to develop a microbial in secticide, B. thdringiensis var. thuringiensis was cultured in the medium composed of cocoon-cooked water from a filature. The results obtained are summarized as followss : (1) Bacillus thuringiensis is a bacterium producing a ${\delta}-endotoxin$ especially toxic to lepidopterous insects and a thermostable exotoxin harmful to dipterous insects. (2) With a view to utilizing the cocoon-cooked water discarded from the filature, as a nutrient source in the B. thuingiensis culture, it was analyzed to contain large amounts of various minerals and protein (7.5 mg/ml) believed to be extracted from the pupae. (3) A large amount of the ${\delta}-endotoxin$ can be obtained most cheeply by using cocoon-cooked water instead of distilled water in preparing GYS and citrate salts media. (4) The largest amount of a mixture of the vegetative cells, spores, and crystals was obtained by addition of 8 gr/l of glucose to the GYS medium. (5) The growth of the bacterium was far better, when leucine, isoleucine, and valine were added all together to the citrate salts medium to the concentration of $1.25{\times}10^{-3}M$. (6) The best growth was observed by addition of Na-glutamate to the citrate salts medium to the concentration of $2.5{\times}10^{-3}M$. (7) The optimal culture time ranged from 9 to 15 days. (8) The highest mortality was shown in Pieris rapae Linne with a pH of the total body extract of 8.4, whereas Dendrolimus spectabilis Butler and Bombyx mori Linne with lower pH's were less susceptible to the ${\delta}-endotoxin$. (9) The presence of the thermo stable exotoxin was confirmed by the fact that the supernatant of the culture was very toxic to the Drosophila melanogaster tested.
This study aims to analyze 19 kinds of mineral contents in hair targeting 311 people in Seoul who suffer from hair loss. The results are as follows: 1. The average contents of all kinds of minerals were within the standards. 2. Sodium, potassium, and selenium were more detected in men than in women; calcium, magnesium, copper, and manganese were more detected in women than in men; toxic elements, mercury, antimony, and arsenic were more detected in men than in women, which was statistically significant. 3. As the age increased, potassium (p<.05), iron (p<.01), manganese (p<.05), chromium (p<.01), and mercury (P<0.01) increased gradually, which was statistically significant. In particular, the content of mercury exceeded its standard in those over fifty. 4. Selenium (p<.01), lead (p<.05), aluminum (p<.05), and arsenic (p<.01) were more detected in natural hair than in permanent or colored hair. Calcium (p<.001), magnesium (p<.001), and manganese (p<.01) were more detected in permanent or colored hair than in natural hair. They were all statistically significant.
Arsenic and its compounds which is one of the most toxic elements that can be found naturally on earth in small concentrations are used in the production of pesticides, herbicides, and insecticides. Most arsenic that cannot be mobilized easily when it is immobile is also found in conjunction with sulfur in minerals such as arsenopyrite (AsFeS), realgar, orpiment and enargite. In this investigation we observed the leaching of arsenic in soils amended with several levels of gravel size of arsenopyrite collected from a road construction site. Soil and gravel size of arsenopyrite were characterized by chemical and mineralogical analyses. Results of XRF analysis of arsenopyrite indicated that the proportion of arsenate was 0.075% (wt $wt^{-1}$) while the maximum amount of arsenic in soil samples was 251.3 mg $kg^{-1}$. Cumulative amounts of effluent collected from the bottom of the soil column for different mixing rate of the gravel were gradually increased where proportion of the gravel mixed was greater than 70% whereas the effluent was stabilized to the maximum after approximately 45 pore volumes of effluent or greater were collected. The arsenic in the effluent was recovered from the soil columns in which the proportion of arsenopyrite gravel was 60% or greater. The total amount of arsenic recovered as effluent was increased with increasing proportion of gravel in a soil, indicating that the arsenic in the effluent was closely related with gravel fraction of arsenopyrite.
One of the characteristics features of Chinese Medicine is recognized as the practical use of combinations of crude drugs in the forms of various prescriptions according to the symptoms of illness. Such crude drugs are mostly dried plant roots, rhizomes, leaves, fruits and seeds, sometimes dried animla organ preparations even fossils or minerals. In the oldest book of Chinese Medicine, Sang Han Ron, 113 prescriptions were described in which about 80 crude drugs were included, and in other old literature, Shin Nong Bon Tcho Keun, 363 crude drugs were recorded. Such drugs, in particular, those included in Sang Han Ron are generally low toxic, and the prescriptions are acting mild with less side effects, but obviously not so easy task to determine the effect of the indivisual drug from the total effects of various prescriptions which are generally consisted of many kinds of crude drugs. About 200 years ago, in the middle of Edo era, a Japanese medical doctor, Yoshimasu Todo wrote a series of books named "Yakucho", in which he described the essential various prescriptions in which the certain drugs plays the main role. This book is very suggestive to evaluate the effects of some Chinese drugs. The actual effects of Chinese medicine and drugs have widely been recognized by the East Asian peoples during their long age experiences, but still it has not been studied sufficiently by modern scientific methods to give generally acceptable evidences. During past some years, my collaborators and I have been engaged to study some well-known Chinese drugs chemically to find out the active principles in determining their chemical structures under the collaboration of pharmacologists, Prof. K. Takagi and his coworkers. In the present lecture, I would like to present two topics from our recent investigations: The studies on the principles of Paeony and Ginseng roots.
Kim, Yumi;Oh, Jong-Min;Jung, Hea-Yeon;Lee, Seung Yeop;Roh, Yul
Economic and Environmental Geology
/
v.47
no.4
/
pp.431-439
/
2014
The purposes of this research were to investigate the enrichment of metal-reducing bacteria from KURT groundwater and the identification of the microbial diversity by 16S rRNA as well as to examine microbial Fe(III)/Mn(IV) reduction and to analyze morphological features of interactions between microbes and precipitates and their mineralogical composition. To cultivate metal-reducing bacteria from groundwater sampled at the KURT in S. Korea, different electron donors such as glucose, acetate, lactate, formate, pyruvate and Fe(III)-citrate as an electron accepter were added into growth media. The enriched culture was identified by 16S rRNA gene sequence analysis for the diversity of microbial species. The effect of electron donors (i.e., glucose, acetate, lactate, formate, pyruvate) and electron acceptors (i.e., akaganeite, manganese oxide) on microbial iron/manganese reduction and biomineralization were examined using the 1st enriched culture, respectively. SEM, EDX, and XRD analyses were used to determine morphological features, chemical composition of microbes and mineralogical characteristics of the iron and manganese minerals. Based on 16S rRNA gene analysis, the four species, Fusibacter, Desulfuromonas, Actinobacteria, Pseudomonas sp., from KURT groundwater were identified as anaerobic metal reducers and these microbes precipitated metals outside of cells in common. XRD and EDX analyses showed that Fe(III)-containing mineral, akaganeite (${\beta}$-FeOOH), reduced into Fe(II)/Fe(III)-containing magnetite ($Fe_3O_4$) and Mn(IV)-containing manganese oxide (${\lambda}-MnO_2$) into Mn(II)-containing rhodochrosite ($MnCO_3$) by the microbes. These results implicate that microbial metabolism and respiratory activities under anaerobic condition result in reduction and biomineralization of iron and manganese minerals. Therefore, the microbes cultivated from groundwater in KURT might play a major role to reduce various metals from highly toxic, mobile to less toxic, immobile.
The soil developed from volcanic ash in Jeju Island, Korea, were classified as typical Andisols. The soils had acidic pH, high water contents, high organic matters and clay-silty textures. The crystalline minerals of the samples were mainly composed of ferromagnesian minerals such as olivine and pyroxene, and iron oxides such as magnetite and hematite derived from basaltic materials. A large amount of gibbsite was found at the subsurface horizon as a secondary product from the migration of excessive aluminum. In addition, our study has shown that considerable amounts of poorly ordered minerals like allophane and ferrihydrite were present in Jeju soils. The contents of $SiO_2$ were lower than those of other soil orders, but $A1_2O_3$ and $Fe_2O_3$ contents were higher. These results are some of the important chemical properties of Andisols. The contents of heavy metals were in the range of $84{\sim}198$ for Zn, $56{\sim}414$ for Ni, $38{\sim}150$ for Co, $132{\sim}1164\;mg\;kg^{-1}$ for Cr, which are higher than the worldwide values in most of the soils. Some soil samples contained relatively high levels of Cr exceeding 1000 mg/kg. Mean reduction capacity of the Jeju soils was $6.53\;mg\;L^{-1}$ reduced Cr(VI), 5.1 times higher than that of the non-volcanic ash soils from inland of Korea. The soil reduction capacity of the inland soils had a good correlation with total carbon content (R = 0.90). However, in spite of 20 times higher total carbon contents in the Jeju soils, there was a week negative correlation between the reduction capacity and the carbon content (R = -0.469), suggesting that the reduction capacity of Jeju soils is not mainly controlled by the carbon content and affected by other soil properties. Correlations of the reduction capacity with major elements showed that Al and Fe were closely connected with the reduction capacity in Jeju soil (R = 0.793; R = 0.626 respectively). Moreover, the amounts of Ni, Co and Cr had considerable correlations with the reduction capacity (R = 0.538; R = 0.647; R = 0.468 respectively). In particular, in relation to the behavior of redox-sensitive Cr, the oxidation of the trivalent chromium to mobile and toxic hexavalent chromium can be restricted by the high reduction capacity in Jeju soil. The factors controlling the reduction capacity in Jeju soils may have a close relation with the andic soil properties explained by the presence of considerable allophane and ferrihydrite in the soils.
Journal of The Korean Society of Grassland and Forage Science
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v.26
no.1
/
pp.15-24
/
2006
This study was conducted to make a rapid and easy determination for the fertility of liquid pig manure as fertilizer by investigating the contents, and correlation coefficients of various nutrients. Samples were collected from 118 local pig farms in the western area of Jeju in Korea. Electrical conductivity(EC), dry matter(DM), $NH_4-N$ and minerals were determined and the relationships among them were examined. The collected liquid manure samples from 118 pig farms were classified according to the level of DM contents ;< 3% (92 farms), $3{\sim}6%$ (18 farms), $6{\sim}9%$ (5 farms) and>9% (3 farms), based on the collected data, most of the liquid manure coming from the local pig farms contain small amount of dry matter. The dry matter contents appeared highly correlated(p<0.01) with EC, $NH_4-N$, T-P, Ca, Mg and Na, except for K. In addition EC was proportional to $NH_4-N$, T-P, Ca, and Na except fer Mg. The fertilizer component ratio of $NH_4-N$, P and K in liquid pig manure were not constant, resulting in low efficiency for fertilizer. However, the toxic heavy metals of Cu etc. were below the criteria of organic fertilizer and soil contamination evaluation. Therefore, we concluded that both dry matter content and electrical conductivity could be used as an indicator for evaluating the fertility of liquid pig manure.
The medical mineral menas a single mineral or a complex of minerals. It is natural material. using the medical action of he major or the minor elements, and traditional medicine stuff which has been used since long time ago. Jusa, cinnabar as the mineral name, is the product of the hydrothermal process. It is used to relax the body and cure high blood pressure, apoplexy and cardiopathy. Jusais the major component of "An shin hwan" and "Woo hwang chung shim hwan" nowadays because it has such an excellent calm effect. In addition, it is used to cure cancers such as esophageal cancer and gastric cancer. Jusa composed of mercuric sulfide causes mercury poisoning such as Minamata disease. It is dealt with mineralogical property and chemical composition medical stuff in Korea and China, as well asmercury poisoning and medical action of Jusa in this study. In order to predct accumulation of the interior of the body of the major and minor elements in Jusa, leaching experiment of Jusa by artificial gastric juice was done as well as thermodynamic reaction modelling to know concentration of each species of body fluid. The minor elements of 24 species such as As, Pb, Cd, a and Fe by leaching reaction of Jusa and artificial gastric juice were leached. We can know the fact that as is less than 1 ppm, Hg is less than 25 ppm and Cd and m are not detected. In addition, mercury exists as species of Hg2+, HgCl+, HgCl2, HgCl3-, HgCl42-, HgClOH, HgS(H2S)2, Hg(HS)3-, HgS22-, HgOH and Hg(OH)2 by reaction modelling between Jusa and artificial gastric juice. The concentration of sulfide complexes is 24.2 ppm and that of others is less than 10 ppm. According to increasing pH, the concentration of HgS(H2S)2, Hg(HS)3+, HgS22- and Hg(OH)2 increases, whereas the concentration of HgCl+, HgCl2, HgCl3- and HgCl42- decreases. Therefore, Jusa is very useful for the development of new medicine because it is possible to predict formation of the body species and species accumulation on mercury known as a toxic element and concentration changes of toxicity and efficiency.city and efficiency.
TMA(Tissue Mineral Analysis) is very popular method in hair mineral analysis for health care professionals in over 48 countries medical center. Assesment of nutritional minerals and toxic elements in the hair is very important not only for determining adequacy, deficiencies and unbalance, but also for assessing their relative relationships in a body. In Korea, there are some problems in TMA method. Because of not haying a medical information database which is suitable for korean to do analyze, the requested TMA has to send to TEI-USA. However, as the TMA results from TEI-USA is composed of English documents and graphic files prohibited to open, its usability is very low and a lot of dollars has to be payed. Also, it can make some problems in the reliability of the TMA results, since the TMA results are based on the database of western health and mineral standards, To solve these problems, I developed the first Medical Information System of TMA in Korea here. The system can analyze the complex tissue mineral data with multiple stage decision tree classifier. It is also constructed with multiple fuzzy database and hence analyze the TMA data by fuzzy inference methods. The effectiveness test of this systems can be shown the increased business efficiency and satisfaction rate 86% and 92% respectively.
Cadmium occurs as a minor element in sphalerite ((Zn, Fe)S) from the Boseong-Jangheung gold-silver mine district. We analyzed the abundance of cadmium in sphalerite using an electron probe micro analyzer (EPMA) and discussed the natural sources of cadmium in terms of bedrock geochemistry, in order to preliminarily reconnoiter the potential cadmium contamination in mine districts. Cadmium contents of sphalerites from the Au-Ag mines (Bodeok, Mundeok, Jeonbo, Boknae, Keumsan) in the Boseong-Jangheung district are considerably high, compared with cadmium contents of sphalerites (average = 0.5 wt.% Cd, maximum = 4.4 wt.% Cd) in the world. Sphalerites from the Keumsan mine (average = 9.49 wt.% Cd, maximum=11.22 wt. Cd) are highly enriched in cadmium. Our data suggest that the Boseong-Jangheung area is an important potential site of cadmium contamination in Korea. Based on bedrock geochemistry, natural causes of cadmium enrichment in sphalerite from the mine district are thought to be the mixing of cadmium leached from organic-rich, metasedimentary rocks (including coal) and/or black shales. From this study, we propose that the pinpointing of potential sites of pollution by toxic heavy metals can be done effectively through detailed reconnaisance study on mineralogical compositions of ore minerals such as sphalerite from the mine area.
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