• 한국식품과학회지
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• 제45권6호
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• pp.667-674
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• 2013

본 연구는 열풍 건조와 동결 건조한 오미자를 시료로 하여 마이크로웨이브 추출 조건(W, 시간, 에탄올 농도)에 따른 추출물의 항산화 특성을 비교하였다. 추출물의 총 폴리페놀 함량과 항산화능(DPPH, ABTS radical 소거능)은 마이크로웨이브 용량(100-200W)이 증가할수록 동결 및 열풍 건조 모두에서 증가하였다. 추출시간(1-8분)에 따른 총 폴리페놀 함량과 항산화능은 동결건조 시료에서는 증가하였지만, 열풍건조 시료의 경우는 4분까지 증가하다가 이후에는 감소하였다. 용매를 달리한 추출물의 총 폴리페놀 함량과 항산화능은 에탄올 및 물 혼합 용매 추출물(50, 70%)에서 가장 높게 나타났으며, 열수 추출물과 99% 에탄올 추출물은 낮게 나타났다. 건조방법에 따른 총 폴리페놀 함량과 항산화능을 비교하였을 때, 모든 추출 조건에서 동결건조 오미자는 열풍건조 오미자보다 높은 항산화 활성을 나타내었다. 각 추출조건별 실험인자들의 상관성을 분석한 결과, 동결 오미자는 총 폴리페놀 함량과 항산화 활성 간에 높은 상관성을 나타낸 반면, 열풍건조 오미자는 상관성이 상대적으로 낮았다. 이상의 결과에서 건조방법은 오미자 추출물의 항산화적 특성에 유의적인 영향을 미쳤으며, 동결건조 오미자는 열풍건조 오미자보다 높은 총 폴리페놀 함량과 항산화 활성을 나타내었다.

• 한국약용작물학회지
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• 제14권6호
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• pp.322-328
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• 2006

Green tea is of particular source as it has been found to have strong antioxidant activities. The extracts of green tea during the commercial harvest seasons from April, 2003 to August, 2003 were compared. The purpose of this study was to determine the correlation between the polyphenol content of green teas and its antioxidant activities. The con-tent of total polyphenols was analyzed and several antioxidant testings were performed. The levels of total polyphenols were higher in the green teas (e.g. Woojeon, Sejak) harvested during very early spring and lower in the green teas harvested late(eg. Ipha, Yepcha). In particular, the free radical scavenging, the inhibition of LDL oxidation, the cytoprotective effect and the inhibition of DNA damage were correlated with the total polyphenol contents of green tea extracts harvested early spring such as Woojeon, Sejak and Jungjak. The results obtained here show that all extracts of green teas including purified green tea catechin, GTC, have strong antioxidant activities on oxidative stress in vifrθ. The variation in polyphenol content and antioxidant activities among various types of green tea by the harvesting time may provide critical information for investigators and consumers using tea in purposes of nutrition and chemoprevention.

• 한국작물학회지
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• 제61권1호
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• pp.33-40
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• 2016

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of $8.57{\mu}g/mg$ and $3.83{\mu}g/mg$, respectively while the highest carotenoid content was found in MJ ml/L treatment with $1.62{\mu}g/mg$. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

• Preventive Nutrition and Food Science
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• 제14권3호
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• pp.195-200
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• 2009

This study was carried out to investigate the biological activities of star anise extracts obtained from four different solvents (water, methanol, ethanol and chloroform) by measuring total polyphenol and flavonoid contents, DPPH radical scavenging, hydroxyl radical scavenging, nitrite scavenging activity and antimicrobial activity. The methanol extract showed the highest extraction yield, followed by ethanol, water and chloroform. The properties of the extracting solvents significantly affected the total polyphenol content. Methanol extracts contained more total polyphenols than any other extracts. The highest DPPH radical scavenging activity was found in methanol extract. The hydroxyl radical and nitrite scavenging activity were highest in methanol and ethanol extracts. In antimicrobial activity, water extract showed stronger activities than methanol and ethanol extract against Micrococcus luteus and Bacillus subtilis, and no inhibitory effects on Gram negative bacteria were found in all extracts at the concentration used.

• 한국식품영양학회지
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• 제33권5호
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• pp.561-569
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• 2020

In this study, the antioxidative activity of Jeolpyun containing S. glauca (Bunge) Bunge powder (2%, 4%, 6%, and 8%) and extract (2%, 4%, 6%, and 8%) was evaluated based on the total polyphenol contents, electron-donating ability, scavenging of superoxide anion radical, and decomposition of hydrogen peroxide. Total polyphenol contents of Jeolpyun containing 8% S. glauca (Bunge) Bunge powder were the highest with a value of 2,280±0.00 ㎍ GAE/mL. It was observed that total polyphenol content exhibited a significant relation with concentrations at p<0.05. The electron-donating ability of Jeolpyun containing 8% S. glauca (Bunge) Bunge extract was the highest with a value of 83.93±0.69%. The electron-donating abilities revealed significant differences between concentrations (p<0.05). The superoxide radical scavenging ability of the blank was 0.01523±0.00068 and Jeolpyun containing 6% S. glauca (Bunge) Bunge powder was the highest with a value of 0.00977±0.00842. The hydrogen peroxide decomposing ability of Jeolpyun containing 0% S. glauca (Bunge) Bunge powder was -0.00377±0.0049; 8% S. glauca (Bunge) Bunge powder revealed the highest decomposing ability of -0.0476±0.0234. Hydrogen peroxide decomposing abilities revealed significant differences between concentrations (p<0.05). Jeolpyun containing S. glauca (Bunge) Bunge powder and extract demonstrated improved antioxidative activities.

• 한국식품과학회지
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• 제37권2호
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• pp.268-273
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• 2005

끓는 물에 의한 재래적인 방법과 전자렌지를 이용하여 데치는 방법이 시금치의 phytochemical 성분 및 위해성 요인의 변화에 미치는 영향을 살펴보았다. ${\beta}$-Carotene과 chlorophylls 함량은 데치기에 의해 대조군보다 유의적으로 증가하였으며, 특히 재래적인 방법이 전자렌지에 의한 방법보다 높은 함량을 보였는데 chlorophyll a 함량은 유의적으로 증가한 반면 ${\beta}$-carotene과 chlorophyll b 함량은 단지 함량만 증가하였을 뿐 유의적인 차이는 없었다. Ascorbic acid와 총 flavonoid 및 총 polyphenol 함량은 데치기에 의해 모두 감소하였으며, 전자렌지에 의한 방법이 재래적인 방법보다 높은 함량을 보였다. 이때 전자렌지에 의해 데쳤을 경우 ascorbic acid는 대조군과 유의적인 차이 없이 감소하였으나, 총 flavonoid와 총 polyphenol은 대조군보다 유의적으로 낮은 함량을 보여 다소 영향을 받는 것으로 나타났다. 총균과 대장균군 수는 데치기에 의해 모두 유의적으로 감소하였으며 특히 끓는 물에 의한 재래적인 방법이 전자렌지에 의한 데치기보다 약 20-25% 정도 유의적으로 낮았다. Nitrate는 데치기에 의해 감소하는 경향을 나타냈으며 특히 전자렌지에 의한 방법이 재래적인 방법보다 낮은 함량을 보였다.

• 한국식품영양학회지
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• 제32권6호
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• pp.581-588
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• 2019

In this study, S. glauca (Bunge) Bunge extract was testified total polyphenol contents, electron donating ability on DPPH, and scavenging ability of superoxide anion radical and hydrogen peroxide. Total polyphenol contents of S. glauca (Bunge) Bunge extract were 7,053.30±915.93 ㎍ GAE/mL. In the fractions, fraction by ethanol was the highest content of 10,973.30±1,000.24 ㎍ GAE/mL. Fractions of ethyl acetate and water were 2,386.70±166.53 ㎍ GAE/mL, 2,413.30±41.63 ㎍ GAE/mL respectively. It was shown that total content of polyphenol according to solvent was significant relation at p<0.05. In the experiment of the electron donating ability, 70% ethanol extract and methylene chloride fraction were -246.15±24.17%, -254.01± 16.54% respectively. In case of ethyl acetate fraction, it was the highest electron donating ability to DPPH radical, 39.06±0.34%, and then water fraction of 36.71±5.55%, ethanol fraction of 29.77±2.57%, gradually. Electron donating abilities revealed significant difference (p<0.05) between the solvents. The superoxide radical scavenging ability of standard material was 0.029±0.0011; 70% ethanol extract of 0.022±0.00052, methylene chloride fraction of 0.027±0.00031, ethyl acetate fraction of 0.024±0.0011, ethanol fraction of 0.021±0.00024 and water fraction of 0.024±0.00019. Hydrogen peroxide scavenging ability of 70% ethanol extract was -0.0029±0.00040 and the others were as follow; methylene chloride fraction (-0.0042±0.00058), ethyl acetate fraction (-0.003± 0.0041), ethanol fraction (-0.0029±0.0015) and water fraction (-0.0028±0.00090).

• 한국잠사곤충학회지
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• 제53권2호
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• pp.130-134
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• 2015

본 연구에서는 벼메뚜기의 생리활성을 확인하기 위하여 벼메뚜기의 성충을 실험재료로 하여 항산화 활성, 항균활성, 항균 물질의 열 안정성, pH 안정성, total polyphenol 함량 등을 측정하였다. 추출용매에 따른 항균력은 E. coli 균주의 경우 ethanol 혼합용매에서 가장 높은 항균력을 보였다. 항균 활성은 열풍건조 $40^{\circ}C$와 동결건조 조건에서 가장 높았으며 암수에 따른 차이는 없었고 항균 물질은 열과 pH에 안정하였다. 벼메뚜기의 항산화 활성은 50% ethanol 추출물과 70% ethanol 추출물에서 가장 높은 활성이 나타났고 total polyphenol은 12 ~ 17 mg/100 g으로 건조조건이나 성별에 따른 차이는 크지 않음을 확인하였다.

• 한국조리학회지
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• 제24권1호
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• pp.73-81
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• 2018

The objective of this study was to investigate their physicochemical properties and antioxidant activities of white radish teas such as pH, color value, total polyphenol contents, total flavonoids, DPPH radical scavenging activity, reducing power and sensory evaluation. They were processed by drying(AD), drying and roasting(ADR), steaming and drying(SAD), and steaming, drying, and roasting(SADR). As a result, the pH of ADR, and SADR tended to be significantly lowered and SADR was the lowest at $5.48{\pm}0.14$(p<0.001). Additionally, ADR and SADR were significantly different in color and browning value, compared to AD and SAD. There was no significant difference in reducing sugar content depending on processing methods. The total polyphenol content and total flavonoid content of the teas were significantly increase after roasting. DPPH radical scavenging activity was significantly higher in ADR and SDAR than in AD or SAD by 1.1~1.5 times(p<0.05). Compared with ADR and SADR after roasting, the sensory characteristics of AD and SAD were significantly lower than those of roasted ADAD and SAD, respectively. From the above results, it was concluded that the roasting process is a major process that affects the physicochemical quality characteristics and antioxidant activities.

• 한국약용작물학회지
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• 제19권5호
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• pp.354-361
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• 2011

In order to product dry goods of Gastrodiae Rhizoma with high quality, tubers were treated with various dry methods such as cutting, drying methods and steaming. In cutting than no cutting, they were shown shorter drying hours and lower drying yield. Drying hours in freeze drying was shorter than those of hot air and far-infrared ray. Total polyphenol content was higher in cutting than no cutting. According to steaming, it was increased in freeze drying but decreased rapidly in the other dry methods. Among all treatments, hot air dry treatment with cutting and no steaming had the highest total polyphenol content. Gastrodin and 4-hydroxybenzyl alcohol contents generally were similar or higher in cutting than no cutting. By steaming, gastrodin content in freeze drying was decreased but 4-hydroxybenzyl alcohol content was increased. But hot-air and infrared ray dry, they were shown opposite results. Gastrodin and 4-hydroxybenzyl alcohol contents showed opposite reaction to each other and were shown various response by dry methods.