• The Korean Journal of Mycology
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• v.15 no.3
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• pp.187-195
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• 1987

Two kinds of heat treatment method for the selective isolation of soil fungi to eliminate the commonest fungi and also to examine the vertical and seasonal distributions of the fungal population were applied to soil samples from two plots around Seoul area. The incubation method at $42^{\circ}C$ and heat treatment at $70^{\circ}C$ were used in this experiment. In the incubation method, the almost all the fungi isolated from two plots were mesophile, while the thermotolerant fungi was Aspergillus fumigatus and thermophilic fungi were Sporotrichum thermophile and Malbranchea pulchella var. sulfrea. The most dominant species isolated by this method was A. fumigatus. Nine genera and fourteen species were isolated from the two plots, and S. thermophile, Talaromyces ucrainicus,Malbranchea pulchella var. sulfrea were new to Korea. From the selection method by heat treatment at $70^{\circ}C$, ten genera and twenty species were isolated. Among these, the most fungi were also mesophile and thermotolerant fungus was A. fumigatus. The most dominant species isolated by this method was T. stipitatus, Talaromyces helicus var. major, Emericella nidulans var. nidulans, Chaetomium subspirale and Neosartorya fisheri var. fisheri were new to Korea. From the two isolation methods, it was found that the total number of soil fungi and frequency of species appeared including dominant ones were the highest at the soils of upper layer while the lowest at the soils of lower layer in its vertical distribution.

• Korean Journal Plant Pathology
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• v.13 no.1
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• pp.46-56
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• 1997

Based on the principal component analysis (PCA) of Richards' parameter estimates, ginseng field soils were grouped as the principal component 1 (PC1) and the principal component 2 (PC2). The microflora and physico-chemical characteristics of each soil group were compared to elucidate soil environmental factors affecting the disease development of root rot of ginseng seedling. Among 3 soil groups by PC1, there were differences in the populations of total fungi (TF) and Cylindrocarpon plus Fusarium (C+F), and the population ratio of Cylindrocarpon plus Fusarium to total fungi or total bacteria (C+F/TF, C+F/TB) in rhizoplane of ginseng seedlings, the population of total actinomycetes (TA) and the population ratio of total Fusarium to total actinomycetes (Fus/TA) in soil, and soil chemical properties (EC, NO3-N, K, Mn, ect.). Among 4 soil groups by PC2, there were differences in TF, C+F, TB, C+F/TF and C+F/TB in the rhizoplane, Trichoderma plus Gliocladium (T+G) in soil, and P2O5 content in soil. Especially, EC, NO3-N, K, K/Mg and Mn were positively correlated to PC1, and TA was negatively to PC1; however, TF, C+F, TB, C+F/TF and C+F/TB in the rhizoplane were significantly correlated to PC2 positively. On the other hand, microbes in the rhizoplane were not significantly correlated to the stand-missing rate (SMR), although TA and Fe/Mn were negatively correlated, and pH and Ca were positively correlated to SMR.

• Journal of Veterinary Clinics
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• v.7 no.2
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• pp.489-495
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• 1990

The purpose of this study was to investigate the contamination rates of Keratinophilic fungi in soils In Chonju. From september to November 1986, 98 soil samples were collected at the school and children's play ground, dog barn, pig barn, cow barn, horse barn and fowl barn( total 33 sites ). The samples were collected at the different depths(0～2cm, 30cm, 50cm ) in each of the sites, respectively. Each sample was cultured at 25$^{\circ}C$ according to Modified hair baiting method by using horse mane hair as a bait. The results obtained were summerized as follows ; 1. Eighty one of the 98 soil samples were found to be positive for Keratinophilic fungi. 2. In the examination of keratinophilic fungi in 98 soil samples, the organisms isolated weie as follows; Microsporum gypseum 20, Trichophyton ajelloi 15, Chrysosporium tropicum 40, C. Keratinophilum 24, C. tuberculatum 6 and Chrysosporium spp. like organism 4 strains. 3. The positive rates of keratinophilic fungi in each of the depths were 87.9%(29/33) in surface layer, 90.9%(30/33) in middle layer and 68.8％(22/32) in deep layer. 4. The positive rates of Keratinophilic fungi in each of the sites were 100%(3/3) in horse barn 91.7％ (l1/12) in dog barn, 88.9%(16/18) in pig barn, 86.7%(13/15) in cow barn, 76.2%(16/21) in fowl barn and 75.9%(22/29) in school and children's play ground. 5. The isolation rates of M. gypseum, Pathogenic fungi for human and animals, were as follows ; 58.3％ in dog barn(surface layers 100％, middle layers 50％, deep layers 25％) and 16.7％ in pig barn(surface 33.3％, middle 16.7%, deep 0％). In the cow barn, the isolation rates were 13.3%(surface 40.0％, middle 0%, deep 0％) and 10% in horse barn(surface l00％, middle 100%, depp 100%). In the fowl barn, the isolation rates was 19.1%(surface 28.8%, middle 14.3%, deep 14.3%) and 3.5% in school and children's play ground(surface 0％, middle 0％, deep 11.1%).

• The Korean Journal of Mycology
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• v.35 no.1
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• pp.11-27
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• 2007

This study was conducted to investigate the diversity of higher fungi in NaeJangSan National Park from April 2004 to November 2006. The obtained results from investigation were as follows. The total of 5 classes, 19 orders, 60 families, 168 genera and 418 species (including 10 families, 13 genera and 15 species unrecorded) including saprophytic and ectomycorrhizal fungi was investigated. The higher fungi were classified into 47 families, 143 genera and 384 species in Basidiomycotina, 9 families, 19 genera and 28 species in Ascomycotina and 4 families, 6 genera and 6 species in Myxomycota. It was turned out that most of the higher fungi belong to Hymenomycetidae in Basidiomycotina, for which 34 families 122 genera, and 353 species were observed. Dorminant species belonged to Tricholomataceae(64 species) Russulaceae(39 species), Polyporaceae(36 species) and Boletaceae(36 species). The mushroom occurrence of higher fungi was closely related to climatic conditions such as high air temperature and lots of rainfall from July to September. The environment which has a favorable influence of mushroom occurrence was air temperature, relative humidity and rainfall of climatic environment.

• Mycobiology
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• v.31 no.3
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• pp.179-182
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• 2003

A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 11 locations in Korea. Their antifungal activities were screened in vivo by antifungal bioassays after they were cultured in potato dextrose broth and rice solid media. Antifungal activity against plant pathogenic fungi such as Magnaporthe grisea(rice blast), Corticium sasaki(rice sheath blight), Botrytis cinerea(tomato gray mold), Phytophthora infestans(tomato late blight), Puccinia recondita(wheat leaf rust), and Blumeria graminis f. sp. hordei(barley powdery mildew) was determined in vivo by observing the inhibition of plant disease development. Twenty(11.7%) endophytic fungi fermentation broths were able to control, by more than 90%, at least one of the six plant diseases tested. Among 187 liquid broths, the F0010 strain isolated from Abies holophylla had the most potent disease control activity; it showed control values of more than 90% against five plant diseases, except for tomato late blight. On the other hand, fourteen(7.5%) solid culture extracts exhibited potent disease control values of more than 90% against one of six plant diseases. The screening results of this study strongly suggested that metabolites of plant endophytic fungi could be good potential sources for screening programs of bioactive natural products.

• Mycobiology
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• v.44 no.4
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• pp.217-236
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• 2016

Polyporoid and corticioid fungi are among the most important wood-decay fungi. Not only do they contribute to nutrient cycling by decomposing wood debris, but they are also valuable sources for natural products. Polyporoid and corticioid wood-inhabiting fungi were investigated in Odaesan National Park. Fruit bodies were collected and identified based on morphological and molecular analyses using 28S and internal transcribed spacer regions of DNA sequences. As a result, a total of 149 species, 69 genera, 22 families, and 11 orders were recognized. Half (74 species) of the species were polypores, and the other half (75 species) were corticioid fungi. Most of the species belonged to Polyporales (92 species) followed by Hymenochaetales (33 species) and Russulales (11 species). At the genus level, a high number of species was observed from Steccherinum, Hyphodontia, Phanerochaete, Postia, and Trametes. Concerning distribution, almost all the species could be found below 1,000 m, and only 20% of the species were observed from above 1,000 m. Stereum subtomentosum, Trametes versicolor, T. hirsuta, T. pubescens, Bjerkandera adusta, and Ganoderma applanatum had wide distribution areas. Deciduous wood was the preferred substrate for the collected species. Sixty-three species were new to this region, and 21 species were new to Korea, of which 17 species were described and illustrated.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.457-473
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• 2020

We investigated the distribution and diversity of fungi in brackish water and soil from the Eulsukdo Island, Geumgang Estuary Bank, Suncheon Bay, Dae-ho Tide Embankment and coastal sand dune in Sinduri and Bu-nam Tide Embankment, Korea. Fungi were isolated from water samples by hand-pumped filtration, and soil samples were collected and diluted. The isolated fungi were incubated in potato dextrose agar at 25℃. A total of 173 fungal strains were isolated from brackish water and identified according to their respective internal transcribed spacer via phylogenetic analysis. The diversity of all fungal strains was analyzed according to diversity indices. The fungal strains belonged to any of 18 taxonomic orders: Pleosporales, Eurotiales, Capnodiales, Hypocreales, Polyporales, Saccharomycetales, Agaricales, Glomerellales, Mucorales, Dothideales, Russulales, Xylariales, Sordariales, Myrmecridiales, Tubeufiales, Onygenales, Cantharellales, and Amphisphaeriales. Cladosporium spp. (20%), Penicillium spp. (19%), and Fusarium sp. (5%) comprised majority of the identified strains. Two species from the fungal isolates were newly identified in Korea: Sarocladium kiliense NNIBRFG3280 and Fusicolla merismoides NNIBRFG23708.

• Journal of Food Hygiene and Safety
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• v.38 no.2
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• pp.63-68
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• 2023

In this study, we analyzed the effect of storage conditions on the survival of fungi in red pepper powder. Red pepper powder was inoculated with a total of six fungal species, namely Aspergillus terreus, Aspergillus flavus, Rhizopus microsporus, Aspergillus fumigatus, Aspergillus niger, and Aspergillus ochraceus at a final cell count of 4-6 log CFU/g. After inoculating the sterilized red pepper powder with fungi, we dried the powder on a clean bench and packaged it in zipper bags. Following drying, the water activity was 0.502±0.001. Subsequently, the red pepper powder inoculated with fungi was stored at -20℃, 5℃, 15℃, and 25℃. All six species of fungi perished the quickest at 25℃ and survived for the longest (168 days) at -20℃. In summary, this study showed that fungi survive for an extended period in red pepper powder at -20℃ and 5℃ compared to 15℃ and 25℃. Therefore, to prevent fungal contamination, red pepper powder should have a water activity below 0.6 and be stored in a zipper bag at room temperature.

• The Korean Journal of Mycology
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• v.14 no.2
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• pp.109-119
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• 1986

The correlations between environmental influences on microorganisms in soil and its effects on disease development in ginseng field were studied to obtain some useful data for increasing ginseng production and effective preventive measures against the root rot caused by soil-borne pathogens. The diseased replanted ginseng fields were selected as the diseased field and the healthy plot in first planted field selected as control in three major Korean ginseng producing areas such as Kumsan, Goesan and Poonggi. The physicochemical characteristics of the soil were analyzed and microorganisms susceptible for root rot of ginseng, such as Fusarium spp. and general fungi were investigated for their population density in various soil conditions. Correlations between soil microbial populations and environmental factors were investigated. The numbers of Fusarium spp. propagules were abundant in fall in both soil conditions. The numbers of Fusarium spp. were 1.9 to 2.6 times higher in replanted field than first planted field except Goesan area. Relative ratio of Fusarium spp. to total fungi propagules in replanted field was 1.6 times higher in replanted field than first planted field indicating higher numbers of Fusarium spp. distributed in replanted field of soil. The numbers of propagules of total fungi were increased in June and July and there was no sensitive variation according to the temperature. There was no significant difference in vertical distributions of total fungi according to soil depth, while the total fungi were abundant in the surface layer and $10{\sim}15\;cm$ layer. The contents of organic matter and phosphate in healthy field were somewhat high, and phosphate/organic matter ratio and Mg contents were high in diseased field. All of the soils showed a weak acidic pH of 4.5 to 5.7. Soil moisture contents were increased during winter season, but did not show any significant changes during the growing periods, showing 24.6% in healthy field and 19.5% in diseased field respectively. Soil temperature was the highest in July and August and the lowest in January and February.

• Culinary science and hospitality research
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• v.7 no.3
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• pp.85-98
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• 2001

This study was performed to describe the overall sanitation of baking utensils and equipments, employees, and environment in 9 bakeries. Microbiological tests on employees, utensils and equipments, were done according to standard procedure and included total plate count, coliforms, fungi and Staphylococcus aureus.. Microbiological testing is a value in determining hazards for developing a HACCP plan but were not detected throat and employee's hands before use. Staphylococcus aureus was detected nasal cavity and employees's hands after use. Employee's apron after use was detected fungi and coliform and was risk factor of cross-contamination to bread or cookies et al. Generally hygienic conditions of pan, kitchen board, knife, brush, and wooden scoop were worse than those of other baking utensils such as tray, bread tweezers, dusting brush and dish cloth. And refrigerator, freezer and fermentation chamber were detected fungi and coliforms. Total plate count of heating table, working table, distribution table, washbowl and refrigerator was increased in 2nd period. Temperature of refrigerator was 10.43$^{\circ}C$ and strict temperature control of refrigerations needs. Therefore, baking utensils and equipments were reguraly need to sterilize and clean. Additionary, it need to practice the effective sanitation education and training program for the bakery managers and employees.