• 제목/요약/키워드: Total and amino nitrogen

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미나리의 단백질의 및 아미노산 조성 (Protein and Amino Acid Composition of Water Cress Oenanthe stolonifera DC)

  • 문숙임;조용계;류홍수
    • 한국식품영양과학회지
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    • 제19권2호
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    • pp.133-142
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    • 1990
  • This study was attempted to compare the nutritive value of leaf with stem of the water cress Oenanthe stolonifera DC. in order to improve the eating habits and as a part of studying on the effective curing nutrients for the damaged liver. The contents of moisture crude proteinon the effective curing nutrients for the damaged liver. The contents of moisture crude protein crude fat and crude ash were 90.40% 2.85%, 0.42% and 0.74% in leaf while the contents of moisture crude protein crude fat and crude ash were 95.15% 0.77% 0.09%, and 0.64% of moisture crude protein crude fat and crude ash were 95.15% 0.77% 0.09% and 0.64% in stem respectively. The quantitative fractionation of proteini of both leaf and stem ranked albumin the highest content followed globulin prolamin and glutelin in order. It has been sh-own by SDS-polyacrylamide gel electrophoresis that water extractable protein of leaf 11 bands but those of stem were not detected. The scope of molecular weight for the main protein of water extractable protein of leaf was between 34.700 and 45,000. The amounts of extractive-nitrogen from leaf and stem of the water cress were 241.02mg% and 271.67mg% respec-tively. The amounts of free amino acid-nitrogen from the leaf and stem were 89.02mg% and 32.02mg% respectively. In free amino acid-nitrogen from the leaf and stem were 89.02mg% and 32.02mg% respectively. In free amino acid composition of both leaf and stem the major components were aspartic acid and glutamic acid. In total amino acid composition of water cress leaf aspartic and glutamic acid were the major components. Whereas alanine and thr-eonine were the major components in stem The assessment of water cress leaf and stem with chemical score. EAAl Rl showed that the values of stem were lower tendancy than those of leaf. Limiting amino acid of leaf was tryptophan while that of stem was lysine.

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가열처리한 저염 멸치액젓의 저장 중 품질변화 (Quality Changes of Low-Salt Anchovy Sauce Treated by Heating during Storage)

  • 박종혁;김상무
    • 한국수산과학회지
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    • 제38권2호
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    • pp.89-93
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    • 2005
  • To manufacture the low-salt anchovy, Engraulis japonicus, sauces with $14\%$ and $17\%$ NaCl, anchovy sauce with $26\%$ NaCl was diluted with sterilized water and then heated at $60^{\circ}C$ for 10 min, 20 min, 30 min, and 40 min. The chemical and microbial changes of the heat-treated low-salt anchovy sauces stored at $25^{\circ}C$ were analyzed at different storage periods. Lactic acid and total nitrogen contents of $14\%$ and $17\%$ NaCl anchovy sauces decreased slightly during storage, but pH, VBN and amino nitrogen contents were almost constant. The amino nitrogen content of $17\%$ NaCl anchovy sauce was higher than that of $14\%$ NaCl anchovy sauce. Total viable cell and lactic acid bacteria were not detected in the anchovy sauce heated at $60^{\circ}C$ for more than 20 min and only proteolytic bacteria was determined less than 10 CFU/mL. The 17% NaCl anchovy sauce heated at $60^{\circ}C$ for 30 min was relatively good for the taste and odor by sensory evaluation.

고추장의 맛성분(成分)에 관(關)한 연구(硏究) - (제(第)1보(報)) 전(全)아미노산(酸) 함양(含量)과 질소성분(窒素成分) - (Approach to the Taste Components of Kochuzang (Red Pepper Paste) - Part 1 : Content of Amino Acids and other Nitrogen Compounds -)

  • 이택수;조한옥;류명기
    • Journal of Nutrition and Health
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    • 제13권1호
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    • pp.43-48
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    • 1980
  • In order to identify the taste components of Kochuzang (red pepper paste), glutinous rice Kochuzang were prepared with addition of Saccharomyces rouxii and Torulopsis versatilis respectively. The nitrogen compounds were determined during the fermentation period at intervals and the amino acid components of 210 days aged glutinous rice Kochuzang were measured. Results are as follows; 1) The increment of amino-N and soluble-N of Kochuzang were increased remarkably during fermentation period untill 60 day but after that period, the increment was slowly changed. 2) Ammonia-N of Kochuzang was increased slowly during aging period but after that time, the amount was decreased slightly. 3) Amino-N ratio and Nitrogen solubility of 300 days aged Kochuzang were 23.71-25.38% and 54.12-56.19% respectively. 4) 17 Kinds of amino acid were identified at 210 days aged Kochuzang those were Lysine, Histidine, Arginine, Aspartic acid, Threonine, Serine, Glutamic acid, Proline, Glycine, Alanine, Valine, Methionine, Isoleucine, Leucine, Phenylalanine, Tryptophane and Tyrosine. 5) Total amino acids were 3.5-4.1% which consists of Glutamic acid, 0.95-1.05%, Aspartic acid, 0.63-0.65% and Serine, Proline, Alanine, Valine, Leucine were 0.22-0.28% respectively but Methionine, Histidine and Arginine were below 0.1%, and Tyrosine, Tryptophane and Phenylalanine existed in Kochuzang in small quantities.

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Optimized Conditions for Making Tea from Camellia(Camellia japonica) Leaf and Flower and Sensory Evaluation

  • Kim, Ju-Hee;Im, Wha-Chun;Park, Min-Hee-;Lee, Jun-Ho;Lee, Sook-Young
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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    • pp.34-35
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    • 2003
  • Before making of tea, the number of leaf, plant height and node length in young shoot were 5.2, 14.9cm and 1,9cm respectively. These contents caused problems in leaf rolling and uniformity. No significant difference in quality and comoposition of roasted and steamed were observed, external shape and internal quality, however, were good from 1st to 3rd leaf. Chemical nutrition consists of leaf position, Total nitrogen content of terminal leaf was 4.88%, total free amino acid 21.12%, and caffein 3%. Vitamin C content was increased with increasing of leaf age. Making of roasted tea was required long time because camellia leaf was very hard and smoothly. Products had lower water color, perfume and taste. Internal quality of steamed tea was good in water color and taste. The contents of total nitrogen, total free amino acid, catechin, caffeine and vitamin C were 4.24%, 1.01, 17.7%, 2.6% and 75.7mg/ml.(중략)

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호두 첨가에 따른 양조간장의 맛 성분 및 관능적 특성 변화 (Changes in Taste Compounds and Organoleptic Preferences of Soy Sauce with Addition of Walnut)

  • 최희은;유범석;최호민;김준협;정성모;이난희;김나율;최웅규
    • 한국식품영양학회지
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    • 제30권5호
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    • pp.916-922
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    • 2017
  • This study was conducted to investigate changes in organoleptic properties and taste components including free sugar, organic acid and free amino acid with addition of walnut. Changes in total nitrogen were insignificantly different with addition of walnut. Amino type nitrogen content was decreased in proportion to supplemental level of walnut. Content of organic acids, of which three kinds were detected, such as citric acid, malic acid and lactic acid increased more than two times with addition of walnut. Contents of free sugar and amino acid were decreased with addition of walnut. The proportion of essential amino acid was 40.6~41.4 percent. Glutamic acid in total amino acid was increased in proportion with addition of walnut. The highest sensory evaluation score was recorded in soy sauce with addition of 2 percent walnut. Together, it was expected that 2 percent addition of walnut to soy sauce has a positive effect on the taste of soy sauce.

해양심층수 소금으로 제조한 간장의 발효기간에 따른 품질변화 (Changes in the Quality Characteristics of Soy Sauce Made with Salts Obtained from Deep Ocean Water)

  • 권오준;김미애;김태완;김대곤;손동화;최웅규;이선호
    • 한국식품저장유통학회지
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    • 제17권6호
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    • pp.820-825
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    • 2010
  • 본 연구에서는 경제적 측면에서 일반 소금보다 우수한 해양심층수 소금을 이용하여 간장을 제조한 후 발효기간에 따른 품질특성을 조사하였다. pH와 완충능은 발효가 진행됨에 따라 점차 감소하였다. 순추출물의 함량은 점차 증가하여 발효 120일째에 7.5%로 나타났다. 간장의 갈색화는 발효 30일 째에 대부분의 갈색화가 진행되었다. 총질소와 아미노태 질소는 발효가 진행됨에 따라 점차 증가하여 발효 12일째 각각 1.4%와 0.75%를 나타내었다. 유리아미노산의 함량은 179.8~315.7 mg%가 검출되었으며, 발효기간이 증가함에 따라 점차 함량이 증가하였다. 발효 전 기간에 걸쳐 glutamic acid가 가장 많았으며, alanine, lysine 및 leucine의 순으로 많았다. 총 유리 아미노산에 대한 glutamic acid의 비율은 21.0~23.2%였으며, 필수아미노산의 함량은 발효 30일째에 78~142.3 mg%가 검출되었으며, 발효가 진행됨에 따라 그 함량도 증가하였다.

LC-MSMS를 이용한 대기 중 PM2.5 유리아미노산 분석 방법 연구 (Determination of Analytical Approach for Ambient PM2.5 Free Amino Acids using LC-MSMS)

  • 배민석;박다정;이권호;조승식;이광열;박기홍
    • 한국대기환경학회지
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    • 제33권1호
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    • pp.54-63
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    • 2017
  • Atmospheric nitrogen containing organic compounds(e.g. amino acids) has attracted considerable attention from the viewpoint of the oceanic biogeochemical cycle of nitrogen as well as the long range transfer. However, only a few measurements of organic nitrogen compounds have been conducted due to analytical difficulties. In this study, total of nine amino acids such as Glutamic acid, Histidine, Arginine, Tyrosine, Cystine, Valine, Methionine, Phenylalanine, Lysine have been analytically determined by Liquid Chromatography - Mass Spectrometry Mass Spectrometry (LC-MSMS). As results, Fragmentor Voltage (FV), Precursor Ion, Collision Energy, Product Ion related to individual amino acid compounds are shown. Based on the operational conditions, Lysine, Glutamine Acid, Tyrosine were analyzed during the China Oriented Smog Period. High concentrations of Lysine, Glutamine Acid, and Tyrosine are discussed with organic carbon (OC), elemental carbon (EC), and water soluble ions. The results can provide to understand the sources with aging process related to amino acids influenced by the long-range transport from the Yellow Sea area.

Performance of Broiler Chickens Fed Low Protein, Limiting Amino Acid Supplemented Diets Formulated Either on Total or Standardized Ileal Digestible Amino Acid Basis

  • Kumar, C. Basavanta;Gloridoss, R.G.;Singh, K.C.;Prabhu, T.M.;Suresh, B.N.
    • Asian-Australasian Journal of Animal Sciences
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    • 제29권11호
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    • pp.1616-1624
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    • 2016
  • The aim of present experiment was to investigate the effect of protein reduction in commercial broiler chicken rations with incorporation of de-oiled rice bran (DORB) and supplementation of limiting amino acids (valine, isoleucine, and/or tryptophan) with ration formulation either on total amino acid (TAA) or standardized ileal digestible amino acids (SIDAA). The experimental design consisted of $T_1$, TAA control; $T_2$ and $T_3$, 0.75% and 1.5% protein reduction by 3% and 6% DORB incorporation, respectively by replacing soybean meal with supplemental limiting amino acids to meet TAA requirement; $T_4$, SIDAA control, $T_5$ and $T_6$, 0.75% and 1.5% protein reduction by DORB incorporation (3% and 6%) with supplemental limiting amino acids on SIDAA basis. A total of 360 dold fast growing broiler chicks (Vencobb-400) were divided into 36 homogenous groups of ten chicks each, and six dietary treatments described were allocated randomly with six replications. During 42 days trial, the feed intake was significantly (p<0.05) reduced by TAA factor compared to SIDAA factor and protein factor significantly (p<0.05) reduced the feed intake at 1.5% reduction compared to normal protein group. This was observed only during pre-starter phase but not thereafter. The cumulative body weight gain (BWG) was significantly (p<0.05) reduced in TAA formulations with protein step-down of 1.5% ($T_3$, 1,993 g) compared to control ($T_1$, 2,067 g), while under SIDAA formulations, BWG was not affected with protein reduction of 1.5% ($T_6$, 2,076 g) compared to $T_4$ (2,129 g). The feed conversion ratio (FCR) was significantly (p<0.05) reduced in both TAA and SIDAA formulations with 1.5% protein step-down ($T_3$, 1.741; $T_6$, 1.704) compared to respective controls ($T_1$, 1.696; $T_4$, 1.663). The SIDAA formulation revealed significantly (p<0.05) higher BWG (2,095 g) and better FCR (1.684) compared to TAA formulation (2,028 g; 1.721). Intake of crude protein and all limiting amino acids (SID basis) was higher in SIDAA group than TAA group with resultant higher nitrogen retention (4.438 vs 4.027 g/bird/d). The nitrogen excretion was minimized with 1.5% protein reduction (1.608 g/bird) compared to normal protein group (1.794 g/bird). The serum uric acid concentration was significantly reduced in $T_3$ (9.45 mg/dL) as compared to $T_4$ (10.75 mg/dL). All carcass parameters were significantly (p<0.05) higher in SIDAA formulation over TAA formulation and 1.5% protein reduction significantly reduced carcass, breast and thigh yields. In conclusion, the dietary protein can be reduced by 0.75% with TAA formulation and 1.5% with SIDAA formulation through DORB incorporation and supplementation of limiting amino acids and among formulations, SIDAA formulation was better than TAA formulation.

발효온도 및 식염농도가 전갱이 액젓 발효에 미치는 영향 (Effect of Fermentation Temperature and Salt Concentration on the Quality of Jack Mackerel(Trachurns japonicus) Fish Sauce)

  • 김보경;이홍희;정민홍;조영제;심길보
    • 수산해양교육연구
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    • 제24권6호
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    • pp.755-762
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    • 2012
  • Qualities properties of fish sauce made jack mackerel (Trachurns japonicus) at different salt concentrations (25~35%) for 240 days at fermentation temperature ($25{\sim}55^{\circ}C$) were investigated. Total nitrogen content of the fish sauce made jack mackerel at 25% salt concentration after 240 day of fermentation was higher than those of 30%, 35% salt concentration. Total nitrogen content was increased under the same condition as fermentation temperature increased except at $55^{\circ}C$. Amino nitrogen contents at 25% salt concentration after 240 day of fermentation at 35, 45, $55^{\circ}C$ were 949.3, 812.8 and 834.4 mg/100 g, respectively. Those at 25, $55^{\circ}C$ fermentation temperature were 811.2 and 614.8 mg/100 g, respectively. The amino acid nitrogen content at 30 and 35% salt concentration ware lower than 20% salt concentration and that after 240 day of fermentation at $55^{\circ}C$ was lowest. The volatile basic nitrogen content increased during fermentation as fermentation temperature increased. However, increasing salt concentration controlled the formation of volatile basic nitrogen. Histamine content of samples fermented at $25{\sim}55^{\circ}C$ after 240 days were 9~20 mg/kg showing that it was not significantly different among salt concentration. The results indicated that the controlled salt concentration and fermentation temperature could be used as a successful process for fish sauce of jack mackerel as an unused resource.

밭토양 조건에서 질소함량별 유기자원의 질소 무기화율 추정 (Estimation of Nitrogen Mineralization of Organic Amendments Affected by Nitrogen Content in Upland Soil Conditions)

  • 임진수;이방현;강승희
    • 한국환경농학회지
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    • 제38권4호
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    • pp.262-268
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    • 2019
  • 토양에서 유기자원의 무기화 특성을 구명하기 위하여 질소함량별로 볏짚, 우분퇴비, 균배양체, 유박, 아미노볼 등 총 5가지를 각각 처리하여 128일 동안 실내 항온배양실험을 실시하였으며, 이 분석결과를 질소 무기화 모형에 적용하여 유기자원의 질소 무기화 양상을 구명하였다. 항온배양 기간 동안 유기자원의 질소 순 무기화율은 질소함량이 가장 높은 아미노볼에서 가장 높았고, 질소함량이 가장 낮은 볏짚에서 가장 낮았다.잠재적 질소 무기화율은 전질소 함량과는 양의 상관관계(0.96)가 인정되었다. 무기화 속도상수 k는 유기자원의 유기물(-0.96) 및 탄소함량(-0.97)과 음의 상관관계가 인정되었다. 모형에 의해 추정된 1작기 동안의 질소 무기화율은 볏짚 6.6%, 우분퇴비 11.6%, 균배양체 30.9%, 유박 70.7%이었으며 아미노볼은 81.0%를 나타냈다. 질소 무기화율은 유기자원의 종류 또는 질소함량에 따라 다르게 나타나 질소 무기화율을 유기자원의 질소 공급 특성을 결정하는 지표로 사용할 수 있다. 질소함량이낮거나 발효과정을 거치는 퇴비 등의 유기자원은 퇴비화 과정에서 유기태 질소가 안정화되어 질소의 무기화율이 무발효 유기자원보다 낮으므로 시비량 결정시 양분공급의 목적보다는 토양 물리성 개량을 목적으로 사용하는 것이 바람직하다.