• Animal Bioscience
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• v.34 no.10
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• pp.1579-1589
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• 2021

Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10⁶ spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

• The Research Journal of the Costume Culture
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• v.28 no.3
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• pp.356-375
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• 2020

The purpose of this study is to understand the characteristics and cultural values of the headdress among Chinese minorities with diverse ethnic cultures, and to provide various data on the design of clothing accessories. Theoretical considerations about ethnic minorities were addressed through literature and prior research, with data being collected using literature and websites. The results are as follows. First, the most common type of headdress is the horizontal type, which includes the head style, headband, and head scarf. The second most common is the cylinder type, which is a headdress with variations in the shape of a round hat, and has is evident among various minorities. Third, the pagoda type is decorated with ornaments mounted on top of a round shape. Fourth, the square crown type is a piece of wood as a material for a form of the material and for a variety of jewelry and the production of up to meet the women's head of the jewelry. Fifth, head belt-type ornaments consist of a headband and fancy bead ornaments from the bottom of the head. Mongol women usually wear an exaggerated form of this type. Sixth, the head cover type is a head decoration influenced by the dress code of Muslim women. Seventh is the disc type of crown shape worn by the Dai. Next, the ogival type is a cone-shaped headdress hat most commonly seen as head ornament hat among the Dai. Lastly, the sailboat type is the most exaggerated form of hair ornaments found among ethnic minorities.

• Development and Reproduction
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• v.11 no.2
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• pp.97-104
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• 2007

This study describes the spermatogenesis and sperm ultrastructure of the spiny top shell, Batillus cornutus using light and electron microscopy. The spiny top shells were collected by divers in the coastal water of Wandogun, Cheollanamdo, Korea(N $34^{\circ}13'$, E $126^{\circ}47'$) at May 2003. Spiny top shells of $60.0{\sim}69.9\;mm$ in shell height were used in this study. The testis comprises many spermatogenic follicles which contains germ cells in different developmental stages. The primary spermatocytes in the pachytene stage were characterized by synaptonemal complexes. The early spermatids were characterized by appearance of Golgi complex, increased karyoplasmic electron density and tubular mitochondria. In early spermatid the mass of proacrosomal granules consists of numerous heterogeneous granules with high electron density. From the mid-stage of spermiogenesis the well-developed mitochondria aggregate posterior to the nucleus, and surround the proximal and distal centrioles. In this stage, proacrosomal granules are condensed and form a acrosome with thin envelope. During the late spermiogenesis, the acrosome begins to elongate and then became conical. The sperm consists of head, mid-piece and tail. The head comprises a round nucleus and a conical acrosome. Acrosomal rod of microfibrous is observed between nucleus and acrosome. Five mitochondria observed in mid-piece. And tail has the typical "9+2" microtubular system originates from the centrioles.

• Fashion & Textile Research Journal
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• v.16 no.6
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• pp.954-960
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• 2014

This study surveyed high school girls in the Busan, Ulsan, and Gyeongnam areas to study how they wear school uniforms along with their satisfaction levels and demands in regards to functional school uniforms. This study presents data on the requirements for the development of winter school uniforms for high school girls with improved warmth functions. The findings are: First, high school girls purchased school uniforms of different brands and the purchase rates for three brands were 25% to 30% approximately. Most of them spent more than 300,000 KRW and less than 400,000 KRW on school uniforms purchases. Second, the greatest complaints by students about winter school uniforms were the ability to conduct routine activities and poor thermal insulation. The most popular bottom underwear for girls was underpants and leggings. The most popular top underwear was half-sleeved round neck t-shirts and running shirts. Third, a survey about uniform functionality showed that most students used and 'arm pit sweat absorbing pad', 'Waist size adjusting function, and 'inside pockets with zippers'. Satisfaction with functional uniforms was low: less than 3.5 in all questions. Fourth, students were favorable to develop functional uniforms to keep warm in the winter. The bottom needs to be warmer than the top and it is necessary to keep legs warm, especially the thighs and calves.

• Journal of the Korean Society of Costume
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• v.65 no.6
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• pp.79-98
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• 2015

This article studied women's headdresses that appeared in the paintings of Joseon Dynasty. This examined the shapes of women's headdresses painted in genre paintings, record paintings and nectar ritual paintings from Joseon Dynasty and compared them to literatures and relics in order to analyze their types and characteristics. Headdress can be categorized into three types: (1) square cloth worn on the top of the head; (2) a kind of small cap; (3) Cloth that completely covers the head. The first type of headdress resembles the shape of Garima. In the early period of Joseon Dynasty, it was worn not only by Gisaeng, but also by both upper and lower class women. The second type of headdress includes a cap made by connecting several cloths, round cap, and cone-shaped cap. These two types are worn on top of the head. However, sometimes these were worn between the head and the chignon to fix the chignon. Also, these were used as hair accessories for decorative purposes. In particular, old women wore these headdresses and braided their white hair around them to fix the chignon. This way, the headdress not only kept the head warm, but also hid the old women's scanty hair. Headdress was usually made of black fabric and it was a simple hair accessory that replaced the wig. The third type of headdress was widely worn among lower class women. The cloth completely covered the head so that the hair would not fall when working. It also provided protection from cold and hot weather. According to paintings, there were many ways of wearing the cloth around the head.

• Journal of the Korean Society of Clothing and Textiles
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• v.25 no.4
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• pp.719-730
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• 2001

The purpose of this study is to develop the basic patterns of adult womens brassiere therewith, provide for some basic data useful to the brassiere manufacturing which are both functional and fitting. For this purpose, 220 adults women(aged between 20 and 59) were sampled to be measured for their body sizes and tested for their brassiere wearing. The collected data were statistically processed using the SAS 6.12 for Means, Standard Deviation, etc. The results of this study can be summarized as follows; 1. In order to develop the basic patterns of adult womens brassieres, 75A size was selected out of 12 size combination(3 women per 75A size) and then, their brassiere fitting was tested. As a result, the fronts of the basic patterns were found to have such problems as undone front center, poor levelling of upper cups bottom line or lower cups top line, poor shape of upper cups outside top line and shoulder strip position. On the other hand, n the rear side of the basic patterns, the central part of the wing was slightly lifted, while the wing width and straps interval did not befit the full-cup brassiere. The result of this first brassiere fitting test showed that the functionality and aesthetics of the basic pattern brassieres were more or less satisfactory, and thus, the problems were address for the second test. In the second test, the aesthetics, breast-shaping effect and functionality of the basic pattern brassieres were much improved. 2. The basic patterns of adult womens brassieres were characterized by a round cup wired as well as a full cup embracing the entire breasts. Each basic pattern which consisted of upper and lower cups was shaped \"straight(-)\". The basic sizes adopted at the first design stage (see fig. 1-2) was 75A of which was designed as upper foundation basic pattern. At the second stage, the upper body foundation pattern were used to design lower cup, upper cup, front panel and wing in their order.(see fig. 3-5, fig. 8)5, fig. 8)

• Journal of the Institute of Electronics Engineers of Korea TE
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• v.42 no.4
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• pp.27-32
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• 2005

Liquid valve is divided into cylinder and liquid part or composed of a single body structure. It becomes a connected structure to cylinder head after inserting stainless(STS) shaft to Teflon packing. In injecting and intercepting fluid, working efficiency becomes low because of the top and bottom round trip operation the friction between Teflon packing and STS shaft fluid leakage, decline of working environment, and each part replacement. And so target value is unattainable in productivity liquid valve design, quality, and structure change are studied. In this paper, designed to solve the existing problems basically, to prevent friction of Piston by developing diaphragm linked with piston, to satisfy long life, and to provide the prevention of leakage. The objective of the study is also to prevent remains fluid at nozzle tip after dispensing fluid, and bell close with the suction function in piston retreating.

• Journal of the Korea Fashion and Costume Design Association
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• v.17 no.2
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• pp.63-78
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• 2015

The purpose of this study is to compare the style of clothing by type of the Goguryeo and Xianbei Tribe based on the examination of the literature centered on the historical relationship between Goguryeo and Xianbei Tribe. The research results are as follows. First, it was found that both the apparels of Goguryeo and Xianbei Tribe had hemp cloth with the V-neckline with the blocked front part and hemp cloth with the open front that is worn by overlapping the straight collars of the upper garment. Both men and women wore the garment of the straight line collar that is worn by folding towards the left or right, or upper garment of the round shaped collar. Women wore an upper garment with short sleeves on top of the upper garment of Korean traditional clothes while men wore pants for the lower garment, and women wore skirt and apron with triangular cloth decoration on top of the skirt. Second, Goguryeo Anak Tomb No. 3 mural painting(year 357) shows that apparel worn by the lady who is the wife of the owner of the tomb is similar to the apparel worn by the lady in the lacquer painting for folding screen of the Sima Jinlong Tomb ancient painting on the woman of chaste reputation in terms of the form and structure.

• Fashion & Textile Research Journal
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• v.10 no.6
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• pp.947-954
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• 2008

The purpose of this research is to find out related factors to obtain the best collar pattern of the women's tailored jacket by analyzing the difference of the shapes between the drafted pattern and that of being stitched. The experimental conditions established are as follows. The position and angle of its break point, the notch and the width of the collar and lapel are kept unchanged. The collar decrease in neckline edge and the top collar larger than the under collar. The crossing point of gorge line is changed to make the collar's roll line straight. When the pattern is changed to make the outline of the lapel straight, the amount of cloth is changed too. According to the experimental conditions, The experimental clothes using muslin were made to. put on the manikin and the suitable methods of making the collar pattern were decided. In order to find out the influence of fabric materials on the jacket' shapes, Three kinds of wool fabrics were used and the results were evaluated. Summary of the research is as follows. When 0.5cm of the neckline edge is shortened, the standing part of the collar has less wrinkles, and its position looks natural and lastly, the material has slightly affected the collar's condition. It is desirable to make the top collar 0.2cm longer than the under collar. But the length should be different according to the fabric material. It it is thick cloth, the length has to be extended. To make the roll line straight, 0.4cm of the crossing point of gorge line should be corrected. The thick cloth needs more correction. To make the lapel's outline straight, it needs outwardly round 0.2cm more and materials make little difference to affect the shape.

• Food Science of Animal Resources
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• v.28 no.3
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• pp.283-288
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• 2008

Eight subprimal cuts purchased from the branded Hanwoo beef of 3 quality grades ($1^{++}$, $1^+$, 1) at 13 stores were evaluated the tenderness using Warner-Bratzler shear (WBS). The beef frequency ratio (%) depending on postmortem aging periods were investigated. The 37.5 (quality grade $1^{++}$), 45.8 (quality grade $1^+$), and 36.4% (quality grade $1^{++}$) of branded beef were aged for < 7 d, 26.6 (quality grade $1^{++}$), 47.2 (quality grade $1^+$) and 36.4% (quality grade 1) were aged for $7{\sim}13$ d, and 31.3 (quality grade $1^+$), 4.2 (quality grade $1^+$) and 25.8% (quality grade $1^+$) were aged for 14 to 20 d. The temperature of cold room in stores was ranged average 2.27 to $2.42^{\circ}C$. WBS values for ansimsal (tenderloin), witdngsimsal (ribeye), cheggtsal (shortloin), doganisal (knuckle) and moongchisatae (hind shank) from branded Hanwoo beef of quality grade $1^{++}$ were tender than those from branded Hanwoo beef of quality grade 1 (p<0.05). WBS values for ansimsal (tenderloin) were 2.56 (quality grade $1^{++}$), 2.76 (quality grade $1^+$) and 3.10 kg (quality grade 1), respectively, and those for doganisal (knuckle, quality grade $1^{++}$), hongdukesal (eye of round, quality grade $1^+$) and bosupsal (top sirloin, quality grade 1) were 4.76, 4.96 and 5.66kg, respectively (p<0.05). The frequency ratio (%) of WBS < 3.9 kg in the all subprimal cuts from branded Hanwoo beef of quality grade $1^{++}$ were 100 [ansimsal (tenderloin) and cheggtsal (shortloin)], 87.5 [witdngsimsal (ribeye)] and 62.5% [bosupsal (top sirloin)], whereas that of WBS > 4.6 kg were 50.0% [hongdukesal (eye of round) and doganisal (knuckle)]. The frequency ratio of WBS < 3.9 kg in the an subprimal cuts of quality grade $1^+$ were 100 [ansimsal (tenderloin) and witdngsimsal (ribeye)] and 44.4% [cheggtsal (shortloin) and gurisal (chuck tender)], whereas that of WBS > 4.6 kg were 66.7 [hongdukesal (eye of round)], 55.6 [doganisal (knuckle)] and 44.4% [bosupsal (top sirloin)]. The frequency ratio (%) of WBS < 3.9 kg in the all subprimal cuts of quality grade 1 were 88.9 [ansimsal (tenderloin)], 62.5 [cheggtsal (shortloin)] and 44.4% [witdngsimsal (ribeye)], whereas that of WBS > 4.6 kg were 100.0 [doganisal (knuckle)] 62.7 [hongdukesal (eye of round)], 62.5 [gurisal (chuck tender)] and 55.6% [moongchisatae (hind shank)]. From these results, subprimal cuts from branded Hanwoo beef were marketed with short aging periods and high frequency ratio (%) of WBS > 4.6 kg.