• 대한소아치과학회지
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• 제30권2호
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• pp.320-325
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• 2003

이산화탄소 레이저의 출력과 조사시간에 따른 유치 법랑질의 탈회억제 및 재경화 효과를 알아보고자 하였다. 유치 법랑질의 직경 2mm 부위를, 파장 $10.6{\mu}m$ 탈촛점 펄스파 이산화탄소 레이저로 6W 2초 및 3W 8초 조사하고, Diagnodent로 측정하여 탈회억제 및 재경화 효과를 평가하고, 선행 연구의 3W 4초 및 6W 4초 조사 결과와 함께 4개군을 비교하였다. 유치 법랑질을 6W 2초 또는 3W 8초 조사 후 탈회시켰을 때, 조사 전과 조사 후에 비해 탈회 후 측정치가 유의하게 증가하였으며(P<0.05), 군 간에 유의한 차이가 없었다. 4개 군 중 6W 4초 군에서 뚜렷한 탈회억제효과가 있었다. 탈회된 유치 법랑질을 6W 2초 또는 3W 8초 조사하였을 때, 탈회 후에 비해 조사 후 측정치가 유의하게 감소하였으나 탈회 전보다는 유의하게 증가하였고(P<0.05), 군 간에 유의한 차이가 없었다. 4개 군 중 6W 4초 군이 거의 완전한 재경화효과가 있었고, 세 군은 부분적 재경화효과가 있었으며 서로 유의한 차이가 없었다. 치아변색은 6W 4초 조사한 경우에만 나타났다. 우식 억제와 치아변색은 총 조사 에너지보다 출력에 더 의존한다고 사료되었다.

• Journal of Korean Dental Science
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• 제5권1호
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• pp.37-44
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• 2012

Purpose: Among the facilitation of tooth movement in adult orthodontic treatment methods, surgical approaches are gaining popularity but complications following mechanical bone reduction are a problem. In this study, tooth movement was observed after alveolar bone was chemically demineralized to verify whether tooth movement had been facilitated. Materials and Methods: Twelve rabbits were used. In the experimental group, the alveolar bone of the left first molar area was exposed and demineralized. Thirty seven percents phosphoric acid was applied for 5 minutes for demineralization. The opposite first molar area was used as control. Two teeth were pulled with 200 g force and 4 rabbits each were sacrificed at 3, 7, and 14 days after the force was applied. Histologic examination was done with hematoxylin and eosin and tartrate-resistant acid phosphatase staining. Result: The histologic examination results revealed more bone resorption in the demineralized area. As time passed, the number of osteoclasts increased in the compressed area. The amount of tooth movement was larger in the experimental group compared to the control group but the difference was not statistically significant. Conclusion: The demineralization with etchant resulted in limited bone resorption, more tooth movement and less damage of the cementum after applied orthodontic force.

• 치위생과학회지
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• 제19권4호
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• pp.261-270
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• 2019

Background: In this study, four types of effervescent vitamins marketed in Korea were analyzed for their acidity and vitamin content. For this purpose, bovine teeth were immersed in vitamin, and surface microhardness and appearance were measured before and after immersion to evaluate tooth demineralization and erosion. Methods: Bovine permanent incisors with sound surface enamel were cut to 5×5 mm size, embedded in acrylic resin, and polished using a polishing machine with Sic-paper. The prepared samples were analyzed for pH, vitamin content, and surface hardness before and after immersion using a surface microhardness meter. Demineralization of surface dental enamel was observed using a scanning electron microscope. Results: The average pH of the four effervescent vitamins was less than 5.5; the pH of the positive control Oronamin C was the lowest at 2.76, while that of the negative control Samdasoo was the highest at 6.86. The vitamin content was highest in Berocca and lowest in the DM company Multivitamin. On surface microhardness analysis, surface hardness values of all enamel samples were found to be decreased significantly after 1 and 10 minutes of immersion (p<0.05). After 10 minutes of immersion, there was a significant difference in the decrease in hardness between the experimental groups (p<0.05). Scanning electron microscopy observation showed that dental enamel demineralization after 10 minutes of immersion was the most severe in Oronamin C except for Samdasoo, followed by DM company Multivitamin and VitaHEIM. Immersion in BeroNew and Berocca resulted in similar effects. Conclusion: There is a risk of tooth erosion due to decreased tooth surface microhardness when using the four types of effervescent vitamins and vitamin carbonated beverages with pH below 5.5. Therefore, high pH vitamin supplements are recommended to prevent tooth erosion.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제41권2호
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• pp.116-116
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• 2015

• Restorative Dentistry and Endodontics
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• 제26권5호
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• pp.372-378
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• 2001

The aim of this in vitro study was to analyze the composition of human tooth enamel in terms of three components, Ca, P, and F after demineralization and remineralization in acid buffer solution. A total of 8 human premolars without any defects and cracks were selected and buccal and lingual sides of the teeth were cleaned with an ultrasonic device and pumice without fluoride 5$\times$5mm windows were opened, and other areas were completely covered with 3-coats of nail varnish to prevent from being in contact with demineralized and remineralized solutions. After demineralization process, each tooth was sectioned into two slices, highly polished one of them with$\gamma$-alumina, and then analyzed the composition of the demineralized tooth with EPMA(electron probe micro-analyzer). The other slices were put into the remineralized solution for 10 days, polished, and analyzed in the same manner. These data were statistically analyzed with one sample t-test(p<0.05). The results were as follows. 1. Normal tooth enamel consists of 49.76% Ca, 39.80% P, and 0.28% F. 2. After demineralization, percentage of Ca and P ratio were decreased by about 5.57 and 5.07% respectively. Percentage of F ratio was also decreased by about 0.01%, which was not statistically significant. 3. After remineralization, percentage of Ca, P increased about by 4.47 and 4.35% respectively Percentage of F decreased by about 0.01%, which was not statistically significant. In conclusion, remineralized solution used in our study has the potential to induce the uptake the Ca and P into the pore sites of the demineralized enamel. But, in the oral cavity. there were rapid temperature change, organic matrix that inhibits the movement of the ions, and limitation of continuous contact with this remineralized solution. Therefore, further in vivo study is necessary.

• 대한소아치과학회지
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• 제46권3호
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• pp.293-300
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• 2019

고정식 교정 장치 치료의 흔한 부작용으로 구강 위생이 불량한 환자에서 브라켓과 밴드에 인접한 법랑질의 탈회가 발생한다. 이 연구는 불소를 이용한 다양한 법랑질 탈회 예방 방법 중 고정식 교정장치 주위의 탈회 예방에 무엇이 가장 효과적인지 알아보고자 시행되었다. 건전한 표면을 가진 발치된 소의 절치 80개를 실험재료로 하여 4개 군으로 분류하였다 : (I군) 대조군, (II군) V $varnish^{TM}$, (III군) Tooth Mousse $Plus^{(R)}$, (IV군) $Vanish^{TM}$ XT. 각 군 별로 처리 후 인공우식용액에서 탈회시킨 다음 0일, 30일, 60일, 90일에 무기질 소실량과 비커스 표면미세경도를 측정하였다. 무기질 소실량은 IV군에서 가장 적었으며, 그 다음으로는 II군, III군 순으로 적었으며, 유의한 차이를 보였다. 표면미세경도는 IV군에서 가장 적었으며, 그 다음으로는 II군, III군 순으로 적었으며, 유의한 차이를 보였다. 이상의 연구결과, IV군이 브라켓 주위 법랑질 탈회 방지에 가장 우수한 효과를 보였다. III군은 대조군과 비교하여 유의한 법랑질 탈회 예방 효과를 보였지만, 다른 군보다는 그 효과가 적었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제41권2호
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• pp.109-115
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• 2015

Odontoma is the most common odontogenic benign tumor, and the treatment of choice is generally surgical removal. After excision, bone grafts may be necessary depending on the need for further treatment, or the size and location of the odontoma. Although the osteogenic capacity of a demineralized tooth was verified as early as 1967 by Urist and many other investigators, the cumbersome procedure, including a long demineralization time, may be less than comfortable for clinicians. A modified ultrasonic technology, with periodic negative pressure and temperature control, facilitated rapid and aseptic preparation of demineralized teeth for bone grafts. This approach reduces the demineralization time dramatically (${\leq}80$ minutes), so that the graft material can be prepared chairside on the same day as the extraction. The purpose of this article is to describe two cases of large compound odonotomas used as graft material prepared chairside for enucleation-induced bony defects. These two clinical cases showed favorable wound healing without complications, and good bony support for future dental implants or orthodontic treatment. Finally, this report will suggest the possibility of recycling the benign pathologic hard tissue as an alternative treatment option for conventional bone grafts in clinics.

• 대한소아치과학회지
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• 제30권1호
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• pp.153-160
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• 2003

연구의 목적은 이산화탄소 레이저의 유치 법랑질 탈회 억제 및 재경화 효과를 레이저형광측정법을 사용하여 평가하는 것이었다. 사람 유치의 건전 평활면으로부터 법랑질 시편을 제작하여, 코카콜라에 담가 24시간 탈회시키는 전후에 정촛점 연속파 또는 탈촛점 펄스파, 3W 또는 6W로 4초간 직경 약 2 mm의 조사점에 이산화탄소 레이저를 조사하고, Diagnodent 측정을 통하여 탈회억제효과와 재경화 효과를 평가하였다. 탈회된 법랑질에 대한 정촛점 연속파 또는 탈촛점 펄스파 조사의 재경화 효과는 두 군 사이에 유의한 차이가 없었으며 모두 탈회 전과 유의한 차이가 없는 수준으로 감소하였다. 건전 법랑질에 대한 3W 또는 6W 탈촛점 펄스파 조사의 탈회 억제 효과에서 3W군은 탈회 후의 측정치가 유의하게 증가하였고(P<0.05), 6W군은 탈회 후 유의한 증가가 없었다. 탈회된 법랑질에 대한 3W또는 6W 탈촛점 펄스파 조사의 재경화 효과에서 3W군은 레이저 조사 후 유의하게 감소하였으나 탈회 전보다 유의하게 컸고(P<0.05), 6W군은 레이저 조사 후 유의하게 감소하였고(P<0.05) 탈회 전과 유의한 차이가 없었다(P>0.05). 유치 법랑질의 색은 6W로 탈회 후 조사하였을 때 갈색 내지 검은색으로 변색되었고 탈회 전 조사하였을 때 갈색으로 변색되었으며, 탈회 전과 후에 3W로 조사하였을 때에는 색의 변화가 없었다.

• 대한소아치과학회지
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• 제25권2호
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• pp.335-351
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• 1998

Salivary proteins which are produced in the saliary acinar cells have been known to be involved in the Calcium and phosphate metabolism. The acquired pellicle resulting from such metabolism is considered as a secondary defence membrane against tooth caries. In this respect, some proteins included in saliva probably play an important role in the prevention of demineralization in enamel. On the other hand, fluoride has long been known to prevent the demineralization of enamel by the inhibition of the growth of Streptococcus mutans(S. mutans) and by the chemical reaction with calcium and phosphate, Therefore, I have examined the roles of amylase and albumin in the demineralization of enamel and compared these preteins with fluoride in terms of anticariogenic effect. 1. The demineralization caused by S. mutans occurred slowly and progressively for the first 60 min, then the rate of demineralization was accelerated afterwards. 2. pH decreased continuously during the entire period of each experiment. 3. The demineralization was significantly inhibited by the preteatment of amylase and fluoride but albumin had little effect on it. 4. An addition of 0.1 mM lactic acid (final concentration 0.1 ${\mu}M$) caused a rapid increase in calcium concentration reaching a maximum within 10 min. 5. pH decreased rapidly by the addition of 0.1 mM lactic acid and reached a minimum within a few seconds followed by an increase in pH. pH reaced a plateu with 10 min. 6. Fluoride, amylase and albumin played little role in the 0.1 mM lactic acid-induced demineralization. 7. A slow infusion of 0.1 M lactic acid at a rate of 5 ${\mu}l/min$ caused a slower increase in calcium concentration compared with the bolus addition of lactic acid. 8. Fluoride had an inhibitory effect on the calcium release caused by slow infusion of lactic acid while amylase and albumin had no effect on it. These results suggest that fluoride inhibits demineralization by protecting the HA from the acid attack whereas amylase has a direct effect on S. mutans to prevent demineralization.

• 대한치과의사협회지
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• 제58권6호
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• pp.324-335
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• 2020

We investigated whether fluoride varnishes recover the hardness of bovine teeth under 20 days of demineralization/remineralization cycling. The fluoride varnish groups (two commercial fluoride varnishes [V-varnish (Vericom, Korea) and CavityShield (3M ESPE, USA)] and an experimental fluoride varnish including 5 wt.% NaF were compared with a control group without fluoride varnish. Vickers hardness was measured at baseline, 3 days after immersion in caries-inducing solution, 24 hours after application of a fluoride varnish, and after 10 and 20 days of demineralization/remineralization cycling. Afterward, tooth surfaces were observed by scanning electron microscope. After fluoride varnish application and the cycling 10 and 20 days, the experimental varnish group showed the highest hardness, while the CavityShield and the control groups demonstrated the lowest hardness. The experimental varnish group recovered the hardness of the baseline at 24 hours after application of the varnish, while it was recovered after 20 days of the cycling in case of the V-varnish. However, the CavityShield and the control groups did not recover the hardness even after 20 days of the cycling. The experimental fluoride varnish with fast recovery in the hardness of the baseline can be used as an effective fluoride varnish to resist demineralization and to facilitate remineralization.