• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.273-282
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• 2009

DNA marker information is used to identify or distinguish cattle breeds or individual animal. The purpose of this study was to apply Bovine Genotypes Kit Version 1.1/2.1 to bovine DNA samples (National Institute of Animal Science) taken from Australian / American beef (n=148), Holstein beef (n=170) and Hanwoo cattle (n=177) bred in Jeongeub, Jeonbuk, Korea, so that it could distinguish Hanwoo breed. The Bovine Genotype Kits consist of 16 ISAG MS markers, which were used to build a database of genotypes in each group. Genotyping results were analyzed using MS Tool kit and Phylip program to create phylogenetic tree. The GeneClass 2.0 was used to estimate breed identification. These analyses found that this kit had 100% capacity to distinguish Hanwoo beef, 95.3% capacity to differentiate Australian / American beef and 90% capacity to identify Korean Holstein steer beef. Hence, it is expected that 16 commercial microsatellite markers is useful to categorizegenetic characteristics of Hanwoo breed and also identify Hanwoo individuals and the origin of beef. In particular, it is expected that these markers will be advantageous in discriminating domestic Holstein beef from Australian / Americanbeef.

• Journal of the Korean Institute of Landscape Architecture
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• v.36 no.5
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• pp.1-12
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• 2008

Since the late 20th century, post-modern society has needed new styles in environmental design. The land art begun during that time supplied the momentum for the birth of the hybrid environmental design. The new design approach, focusing on land form and landscaping begun with land-form architecture, raised a powerful current of hybridization in the environmental design genres. The new picturesque landscape design distinguished by manipulated land forms and sublimated aesthetics appeared under the influence of land art and land-form architecture. Similarly, landscape urbanism was formed by the fusion of landscape architecture and urbanism. Therefore, the representative hybrid styles in environmental design appear as new picturesque landscape design, land-form architecture and landscape urbanism. With the new, strong interest in land and landscape, this same new interest was given to 'time' on account of the dynamics and indeterminacy of urban society. This new interest in land and time gave rise to new hybrid methodologies for environmental design such as mapping, diagramming and folding. These three tools have been applied most comprehensively in landscape urbanism. The 'fold' is the most popular design tool for most of the hybrid genres. The 'diagram' is the second-most popular design tool mostly for landscape urbanism and land-form architecture. Mapping is being actively applied to landscape urbanism and passively applied in new picturesque landscape design. In general, landscape urbanism seems to be a timely and suitable alternative for contemporary urban society. It displays very high potentials in the regeneration of the locality through the comprehensive hybrid methodology. It is necessary to actively engage in and develop landscape urbanism fit the local needs.

• Journal of Life Science
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• v.22 no.2
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• pp.266-280
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• 2012

Agar is a cell wall component of macro red algae that can be hydrolyzed by agarase. Agarases are classified into ${\alpha}$-agarase (E.C. 3.2.1.158) and ${\beta}$-agarase (E.C. 3.2.1.81), in accordance with their cleavage pattern, and can be grouped in the glycoside hydrolase (GH)-16, -58, -86, -96, and -118 family according to the amino acid sequences of the proteins. Many agarases and/or their genes have been detected, isolated, and recombinantly expressed from bacteria, and metagenomes have their origins in sea and terrestrial environments. Products of agarases, agarooligosaccharides and neoagarooligosaccharides, represent wide functions such as antitumor, immune stimulation, antioxidation, prebiotic, hepa-protective, antibacterial, whitening, and moisturizing effects; hence, broad applications would be possible in the food industry, cosmetics, and medical fields. In addition, agarases are also used as a tool enzyme for research. This paper reviews the sources, purifications and detection methods, and application fields of agarases. The role of agarases in agar metabolism and the function of their enzymatic products are also surveyed.

• Journal of architectural history
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• v.15 no.3
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• pp.27-42
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• 2006

Many contemporary architectural avant gardes tend to use painting as a medium to create architecture which goes beyond the rationalized spatial conception of modem architecture represented by perspectivism. They produce non perspective drawings to represent spatial Ideas, and expand it through poetic imagination to create an unexpected architectural form and space. This paper attempts to analyze the historical origin and background of dominance of drawing in the production of architecture. It was with the invention of perspective that architectural representation became important tool for architectural production. Thereafter, drawing was considered prior to actual building and architecture was considered a three dimensional realization of two dimensional drawing. Modernist avant gardes such as Cubism shattered the rationalized pictorial space of perspective and found a new pictorial space. They tried to extend it to three dimensional space through parallel projection largely based on the Hildebrand's theory of pure visibility. However, due to the ambiguity of the position of the viewing subject, their attempts could not succeed in creating a new architecture. The new architectural avant garde of the 70's rediscovered the early 20th century avant gardes in their attempt to create a new architecture which can register the fragmented spatial condition of contemporary society, and used painting as a medium to create architecture. Their difference from the early avant gardes was that they used poetic imagination rather than parallel projection in the process of projecting three dimensional space and form from the painting. However, their architecture cannot escape the scopic field of perspectivism in that they rely on the picture plane and the distance between object and viewing subject. Therefore, I conclude that in order to create architecture which goes beyond the rationalized space of modern architecture, it is necessary to resort to other tradition of modern architecture than visual one.

• International Journal of Industrial Entomology and Biomaterials
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• v.30 no.1
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• pp.6-16
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• 2015

This study established the genetic characterisation of 10 microsporidian isolates infecting non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) collected from biogeographical forest locations in the State of Assam, India, using PCR-based markers assays: inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD). A Nosema type species (NIK-1s_mys) was used as control for comparison. The shape of mature microsporidian spores were observed oval to elongated, measuring 3.80 to $4.90{\mu}m$ in length and 2.60 to $3.05{\mu}m$ in width. Fourteen ISSR primers generated reproducible profiles and yielded 178 fragments, of which 175 were polymorphic (98%), while 16 RAPD primers generated reproducible profiles with 198 amplified fragments displaying 95% of polymorphism. Estimation of genetic distance coefficients based on dice coefficients method and clustering with un-weighted pair group method using arithmetic average (UPGMA) analysis was done to unravel the genetic diversity of microsporidians infecting Indian muga and eri silkworm. The similarity coefficients varied from 0.385 to 0.941 in ISSR and 0.083 to 0.938 in RAPD data. UPGMA analysis generated dendrograms with two microsporidian groups, which appear to be different from each other. Based on Euclidean distance matrix method, 2-dimensional distribution also revealed considerable variability among different identified microsporidians. Clustering of these microsporidian isolates was in accordance with their host and biogeographic origin. Both techniques represent a useful and efficient tool for taxonomical grouping as well as for phylogenetic classification of different microsporidians in general and genotyping of these pathogens in particular.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.14 no.2
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• pp.33-41
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• 2005

ZnO epilayer was synthesized by the pulsed laser deposition(PLD) process on Al$_2$O$_3$ subsorte after irradiating the surface of ZnO sintered pellet by ArF(193nm) excimer laser. The epilayers of ZnO were achieved on sapphire(A1203) substrate at the 境mperature of 400$^{circ}$C. The crystalline structure of epilayer was investigated by the Photoluminescence and double crystal X-ray diffraction(DCXD). The carrier density and mobility of ZnO epilayer measure with Hall effect by van der Pauw mothod are $8.27\times$1016cm$^{-3}$ and 299 cm$^{2}$/V$\cdot$s at 293 K respectively, The temperature dependence of the energy band gap of the ZnO obtained from the absorption spectra was well described by the Varshni's relation, E$_g$(T)= 3.3973 eV - ($2.69\times$ 10$^{-4}$ eV/K)T$^{2}$/(T + 463K). After the as-grown ZnO epilayer was annealed in Zn atmospheres, oxygen and vaccum the origin of point defects of ZnO atmospheres has been investigated by the photoluminescence(PL) at 10K. The native defects of V$_{Zn}$, V$_{O}$, Zn$_{int}$, and O$_{int}$ obtained by PL measurements were classified as a donor or acceptor type. In addition we concluded that the heat-treatment in the oxygen atmosphere converted ZnO thin films to an optical p-type. Also, we confirmed that vacuum in ZnO/Al$_2$O$_3$ did not firm the native defects because vacuum in ZnO thin films existed in the form of stable bonds.

• Journal of Korean Medical classics
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• v.30 no.4
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• pp.49-67
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• 2017

Objective : Heavenly stems and Earthly branches is a tool used for understanding the virtue of Yin Yang and Five elements. Korean medicine understands the changes in Wuyun through the Ten Heavenly stems, and understand the changes of Liuqi through the Twelve Earthly branches. An accurate understanding of the definitions of Heavenly Stems and Earthly branches and the concept of each of the 10 stems is of vital importance. Method : The paper first reviews the origin, history, and significance of the Heavenly stems and Earthly branches before studying the definitions of the stems and branches as laid out in the works of Yu Onseo, Lee Samun, and Han Dongseok. The paper then reviewed the concept of the each of the ten stems through researching the texts of Seoulmunhaeja, the annotations of the four great Seolmuns, and the texts of Jeongyeokwonui. Result & Conclusion : Heavenly stems and Earthly branches have been in use since more than 6,000 years ago. The central numbers in the changes of Heaven and Earth are five and six. Each number functions with duality, yin and yang, meaning there are ten Heavenly numbers ($5{\times}2=10$) and 12 Earthly numbers ($6{\times}2=12$) which oversees all of the cosmic changes. Stems become the body and signifies water. Branches become the use and signifies divided fire. The meanings of the letters Gab Eul Byeong Jeong Mu Gi Gyeong Sin Im Gye originate from the one year life of a tree which grows, bears fruits, processes Yang qi, and awaits for the next spring. The reason a tree is used is because there is nothing better in studying in detail the changes of a living being through a year.

• Economic and Environmental Geology
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• v.28 no.3
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• pp.243-249
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• 1995

In order to investigate the origin of sulfate in rain waters and to evaluate the feasibility of using sulfur isotope method as a tracer of atmospheric pollutants, the sulfur isotopic ratio of sulfate in rain waters collected in Chonju city from October 1994 to March 1995 was monitored and was compared with those of possible sources proposed by previous works. The pH of rain waters shows an intermediate acidic range from 4.45 to 6.88 and their daily variation appears to be well correlated with to the amount of precipitation. The sulfur isotopic ratios of sulfate in rain waters show a highly restricted range from 0.0 to + 1.8‰. The ${\delta}^{34}S$ values are similar to those of soil and pine tree surrounding Chonju city, but largely deviate from those of China. D-parameter($d={\delta}D-8{\delta}^{18}O$) of rain waters varies from 9.4 to 28.8. The values indicate that the rain waters in Chonju city are originated from the rainy front of China continent. All data obtained from this study suggested that sulfate in the rain waters collected in Chonju city was mainly derived from the sulfur dioxide gas emitted by the petroleum combustion. Therefore, sulfur isotopic study for the precipitation provided an excellent tool for environmental assessment in this region and for tracing the source of atmospheric pollutants.

• Journal of Arbitration Studies
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• v.24 no.1
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• pp.85-109
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• 2014

Mediation and Arbitration are two distinct ADR processes. Their dissimilarity lies in the principle that in mediation the parties themselves decide what the resolution to the problem is, whereas in arbitration the arbitrator makes that determination. Med-Arb, hybrid of the two methods, is a fairly new ADR process dating back to the 1970s. Med-Arb capitalizes on the advantages of both mediation and arbitration, while eliminating many of their disadvantages. Mediation has the advantage of allowing for resolutions rather than decisions. Arbitration has the advantage of guaranteeing that the matter will be resolved when the procedure is over. In Med-Arb, the participants agree to be parties to mediation, and if the mediation comes to an impasse, a final settlement will be reached through arbitration. This study first explicates the origin and the development of Med-Arb in the United States. This study shows that the emergence of Med-Arb is benefited from the fact that arbitration has lost its own advantages ie, speed, cost-saving, and maintenance of an ongoing relationship between the disputants. Second, this study analyzes four cases in which Med-Arb is applied to various kinds of disputes as a tool of dispute resolution: labor disputes, entertainment disputes, will disputes, and international commercial disputes, consecutively. All those case studies show the generality of Med-Arb as a dispute resolution channel. Third, this study compares the advantages and disadvantages of Med-Arb. Finally, this study discusses the implications of Med-Arb. In particular it provides the universality of this hybrid form of dispute resolution in the East and West. For example, we show that China has its own distinctive Med-Arb system, where it has developed from ancient Confucian philosophy. Japan also emphasizes the role of an arbitrator who settles the disputes in the course of arbitration. The domestic arbitration rules of the Korean Commercial Arbitration Board (KCAB) have a similar process in that arbitration contains an element of conciliation. With regard to the universal characteristics of Med-Arb, it is necessary to analyze the pros and cons of Med-Arb at a deeper level in the future. One caveat is that it is necessary to handle the issues of the neutrality of the mediator-arbitrator.

• Journal of the Korea Fashion and Costume Design Association
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• v.9 no.1
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• pp.103-122
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• 2007

Fashion have been expressed by a face through various a period, social change, and various culture changing. Human expresses as "Transformation" by applying these needs of changing to the fashion. A origin tribe expressed its own self by using direct body transformation and extreme decorations in the past. However, human express creative and esthetic desire based on shape, material, and transformation method in the present time characteristics of the body. Exceptional transformation breaking a existing fixed idea appeared frequently due to dissolution through transformation which is positive expression method in the fashion from 20th century. As a results, followings are obtained in this study. First, human body transforms by using a tool or intermediation in investigation of aesthetic meaning for human body. The object, Transformation, is stably transformed by disintegration, distortion, exaggeration and simplification reduction, as design's sensitivity. Second, transformation from relation of clothing composition is expressed by extension, reduction, simplification, and dissolution. In transformation from original tribe's sensitivity, past decorative desire lead to transformation of human body. To give variable change from past to present fashion, external formative will is introduced. Then, extreme expression is made by direct transformation of clothing type. It seems to be accomplished that human body's expression method is continuously changed into extension, exaggeration, reduction, and dissolution from transformation method as described before. Transformation of modem fashion is expression method by creative supervision. Extreme transformation substituted body's each part is based on immanent play and representative satisfaction. Through these transformation, it is judged that variety of creative type is achieved.