• International Journal of Precision Engineering and Manufacturing
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• v.9 no.3
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• pp.55-58
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• 2008

Hydroforming is a forming technology in which a steel tube is set in a die and formed to fit a specified shape by applying hydraulic pressure from inside the tube while also applying force in the tube axial direction (axial feed). In present study, the entire design process chain for an automotive cross-member was simulated and developed using hydroforming technology on high-strength steel. The part design stage required a feasibility study. The process was designed using computer-aided design techniques to confirm the actual hydroformability of the part in detail. The possibility of using hydroformable cross-member parts was examined using cross-sectional analyses, which were essential to ensure the formability of the tube material for each forming step, including pre-bending and hydroforming. The die design stage included all the components of a prototyping tool. Press interference was investigated in terms of geometry and thinning.

• Korea-Australia Rheology Journal
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• v.11 no.4
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• pp.305-311
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• 1999

The shear behavior of polymers obtained by means of devices such as capillary and cone-and-plate rheo-meters is commonly used to assess their processing performance and as a characterization tool. However, the number of instances when two polymers have the same shear properties but perform differently during certain types of processing operations (e.g. film blowing and sheet extrusion) indicate that shear properties alone may not be sufficient to characterize polymeric fluids. We begin by defining the kinematics of shear-free or extensional flow and the associated material functions. The extensional and shear behavior of three different types of polyethylene (PE) are then compared to illustrate the points that one cannot ascertain the extensional properties of polymer melts from their shear properties and, furthermore, there may not be a simple relation between properties obtained from one type of extensional flow and those of another type. The kinematics of most processing flows are extensional rather than shear in nature, and , hence, the performance of polymers during processes such as fiber spinning, film casting, film blowing, thermoforming, blow molding, and even extrusion is more readily accounted for through extensional viscosity measurements. Methods for carrying out extensional flow measurements are then reviewed including approximate methods. To illustrate the sensitivity of extensional viscosity measurements to subtle changes in the molecular architecture of PEs, results are presented for samples with a narrow molecular weight distribution but with varying numbers of long chain branches. Finally, constitutive equations which allow one to separate shear and extensional flow behavior are discussed as any attempts to simulate the subtle processing differences between two polymers will require constitutive equations of this nature.

• Journal of IKEEE
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• v.24 no.2
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• pp.486-491
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• 2020

ARM Cortex-M0 DesignStart provided by ARM is cost-free design development suit targeting for designing and prototyping SoC with Cortex-M0 core. In this paper, we presents a method how to implement a custom system design using ARM Cortex-M0 DesignStart. First, hardware elements for ARM Cortex-M0 DesginStart is analyzed focusing on bus and memory map, and next software toolchain is explained to clarify the translating process from high level language to binary machine language. As an example of the custom system, UART system operated with Cortex-M0 is designed and simulated.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.269-269
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• 2017

Germination is one of the techniques used to enhance the texture properties and nutritional value of the brown rice (BR). Therefore, germinated BR (GBR) has received significant attention during the last decade. Physicochemical and cooking properties of brown rice were examined before and after germination. Germination raised the cooking properties, such as water absorption, expanded volume and soluble solid of cooked BR (brown rice). The texture, measured using tensipresser, was significantly improved by germination. The hardness of cooked BR was decreased by germination, but the GBR was sticker. In RVA, all viscosity value (peak viscosity, break down, set back, and final viscosity) of germinated rice flour was also reduced while gelatinization temperature did not change. Amylose content and amylopectin chain length distribution of BR starch were slightly changed by germination. Overall results revealed that germination was an effective tool to improve texture and cooking properties of BR.

• Journal of Korean Society on Water Environment
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• v.24 no.6
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• pp.725-740
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• 2008

In this study a multi-site daily precipitation generator which generates the precipitation with similar spatial correlation, and at the same time, with conserving statistical properties of the observed data is developed. The proposed generator is intended to be a tool for down-scaling the data obtained from GCMs or RCMs into local scales. The occurrences of precipitation are simultaneously modeled in multi-sites by 2-parameter first-order Markov chain using random variables of spatially correlated while temporally independent, and then, the amount of precipitation is simulated by 3-parameter mixed exponential probability density function that resolves the issue of maintaining intermittence of precipitation field. This approach is applied to the Nakdong river basin and the observed data are daily precipitation data of 19 locations. The results show that spatial correlations of precipitation series are relatively well simulated and statistical properties of observed precipitation series are simulated properly.

• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.21-25
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• 2008

Bone marrow mesenchymal stem cells (BMMSCs) have the capacity for self-renewal and differentiation into a variety of cell types. They represent an attractive source of cells for gene and cell therapy. The purpose of this study is to direct the specific expression of the DsRed reporter gene in $Sca-1^+$ BMMSCs differentiated into a cardiomyogenic lineage. We constructed the prMLC-2v-DsRed vector expressing DsRed under the control of the 309 tp fragment of the rat MLC-2v 5'-flanking region. The specific expression of the DsRed reporter gene under the transcriptional control of the 309 bp fragment of the rat MLC-2v promoter was tested in 5-azacytidine healed-$Sca-1^+$ BMMSCs over 2 weeks after the prMLC-2v-DsRed transfection. The prMLC-2v-DsRed was specifically expressed in the $Sca-1^+$ BMMSCs with cardiomyogenic lineage differentiation and it demonstrates that the 309 bp sequences of the rat MLC-2v 5'-flanking region is sufficient to confer cardiac specific expression on a DsRed reporter gene. The cardiac-specific promoter-driven reporter vector provides an important tool for the study of stem cell differentiation and cell replacement therapy in ischemic cardiomyopathy.

• Journal of Korean Society for Quality Management
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• v.44 no.4
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• pp.751-770
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• 2016

Purpose: Two-stage ${\bar{X}}$ chart is a useful tool for process control when a surrogate variable may be used together with a performance variable. This paper extends the two-stage ${\bar{X}}$ chart to a three stage version by decomposing the first stage into the preliminary stage and the main stage. Methods: The expected cost function is derived using Markov-chain approach. The optimal designs are found for numerical examples using a genetic algorithm combined with a pattern search algorithm and compared to those of the two-stage ${\bar{X}}$ chart. Sensitivity analysis is performed to see the parameter effects. Results: The proposed design outperforms the optimal design of the two-stage ${\bar{X}}$ chart in terms of the expected cost per unit time unless the correlation between the performance and surrogate variables is modest and the shift in process mean is smallish. Conclusion: Three-stage ${\bar{X}}$ chart may be a useful alternative to the two-stage ${\bar{X}}$ chart especially when the correlation between the performance and surrogate variables is relatively high and the shift in process mean is on the small side.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.291-291
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• 2010

Electron-beam lithography (EBL) process is a versatile tool for a fabrication of nanostructures, nano-gap electrodes or molecular arrays and its application to nano-device. However, it is not appropriate for the fabrication of sub-5 nm features and high-aspect-ratio nanostructures due to the limitation of EBL resolution. In this study, the precision assembly and alignment of DNA molecule was demonstrated using sub-5 nm nanostructures formed by a combination of conventional electron-beam lithography (EBL) and plasma ashing processes. The ma-N2401 (EBL-negative tone resist) nanostructures were patterned by EBL process at a dose of $200\;{\mu}C/cm2$ with 25 kV and then were ashed by a chemical dry etcher at microwave (${\mu}W$) power of 50 W. We confirmed that this method was useful for sub-5 nm patterning of high-aspect-ratio nanostructures. In addition, we also utilized the surface-patterning technique to create the molecular pattern comprised 3-(aminopropyl) triethoxysilane (APS) as adhesion layer and octadecyltrichlorosilane (OTS) as passivation layer. DNA-templated gold nanoparticle chain was attached only on the sub-5 nm APS region defined by the amine groups, but not on surface of the OTS region. We were able to obtain DNA molecules aligned selectively on a SiO2/Si substrate using atomic force microscopy (AFM).

• Korean Journal of Veterinary Research
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• v.36 no.3
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• pp.627-633
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• 1996

To analyze the genomic diversity of transmissible gastroenteritis virus (TGEV), the N-terminal half of the spike (S) glycoprotein gene and nonstructural protein gene (open reading frames 3 and 3-1) were amplified by reverse transcriptase reaction and polymerase chain reaction (RT-PCR), and analyzed using restriction fragment length polymorphism (RFLP) patterns of the amplified DNA. In this study, TGEV Miller (M6) and Purdue (P115) strains were used as reference strains, and two vaccine strains (MSV and STC3) and four Korea isolates (P44, VRI-WP, VRI-41, and VRI-48) were analyzed. All TGEV strains were amplified with three TGEV primer pairs. Although there was some exception in RFLP analysis, this method differentiated TGEV strains into following groups : Miller group (M6 and MSV), Purdue group (PUS, STC3, P44, VRI-WP, VRI-41, and VRI-48). Using Sau3AI and SspI, VRI-48 was differentiated from the Miller and Purdue type viruses. The RT/PCR in conjuction with RFLP analysis was a rapid and valuable tool for differentiating several strains of TGEV. This study revealed the occurences of distinct difference in genome of TGEV strains.

• Korean Journal of Veterinary Research
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• v.47 no.2
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• pp.163-167
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• 2007

Bovine viral diarrhea (BVD) is very important disease in cattle industry with a worldwide distribution. Detection and elimination of persistently infected calves with bovine viral diarrhea virus (BVDV) was valuable strategy for BVD eradication because those calves were main source for transmission. We surveyed persistent infection with BVDV by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) using whole blood and skin. Five hundred thirty nine Korean native calves were tested. Four calves (0.7%) were positive for BVDV antigen for both tests. Those calves remained positive for follow-up by RT-PCR and IHC. Therefore they were identified as persistently infected with BVDV. We confirmed that immunohistochemistry using skin biopsy samples was very useful tool to detect persistently infected calves with BVDV. As far as we know, this would be first report on persistent infection with BVDV in Korea.