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Development of MURCC code for the efficient multi-unit level 3 probabilistic safety assessment

  • Jung, Woo Sik;Lee, Hye Rin;Kim, Jae-Ryang;Lee, Gee Man
    • Nuclear Engineering and Technology
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    • v.52 no.10
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    • pp.2221-2229
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    • 2020
  • After the Fukushima Daiichi nuclear power plant (NPP) accident, level 3 probabilistic safety assessment (PSA) has emerged as an important task in order to assess the risk level of the multi-unit NPPs in a single nuclear site. Accurate calculation of the radionuclide concentrations and exposure doses to the public is required if a nuclear site has multi-unit NPPs and large number of people live near NPPs. So, there has been a great need to develop a new method or procedure for the fast and accurate offsite consequence calculation for the multi-unit NPP accident analysis. Since the multi-unit level 3 PSA is being currently performed assuming that all the NPPs are located at the same position such as a center of mass (COM) or base NPP position, radionuclide concentrations or exposure doses near NPPs can be drastically distorted depending on the locations, multi-unit NPP alignment, and the wind direction. In order to overcome this disadvantage of the COM method, the idea of a new multiple location (ML) method was proposed and implemented into a new tool MURCC (multi-unit radiological consequence calculator). Furthermore, the MURCC code was further improved for the multi-unit level 3 PSA that has the arbitrary number of multi-unit NPPs. The objectives of this study are to (1) qualitatively and quantitatively compare COM and ML methods, and (2) demonstrate the strength and efficiency of the ML method. The strength of the ML method was demonstrated by the applications to the multi-unit long-term station blackout (LTSBO) accidents at the four-unit Vogtle NPPs. Thus, it is strongly recommended that this ML method be employed for the offsite consequence analysis of the multi-unit NPP accidents.

Construction of a full-length cDNA library from Typha laxmanni Lepech. and T. angustifolia L. from an EST dataset

  • Im, Subin;Kim, Ho-Il;Kim, Dasom;Oh, Sang Heon;Kim, Yoon-Young;Ku, Ja Hyeong;Lim, Yong Pyo
    • Korean Journal of Agricultural Science
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    • v.45 no.4
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    • pp.583-590
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    • 2018
  • Genus Typha L. (Typhaceae; Cattail in common) is one of the hydrophytic plants found in semi-aquatic regions. About nine to 18 species of the genus exist all over the world. In Korea, the most commonly found cattail species are T. laxmanni and T. angustifolia. The aim of this study was to prepare a cDNA library and sequences and analyze expressed sequence tags (ESTs) from these species, T. laxmanni and T. angustifolia. In the case of T. laxmanni, we observed that 715 out of 742 ESTs had high quality sequences, whereas the remaining 27 ESTs were low quality sequences. In this study, we identified 77 contigs, 393 unassembled clones and 65.7% singletons. Furthermore, in the case of T. angustifolia, we recorded 992 high quality EST sequences, and by excluding 28 low quality sequences from among them, we retrieved 120 contigs, 348 unassembled clones and 48.9% singletons. The basic local alignment search tool (BLAST) and Kyoto encyclopedia of genes and genomes (KEGG) database results enabled us to identify the functional categories, i.e., molecular function (16.5%), biological process (22.2%) and cellular components (61.3%). In addition, between these two species, the no hits and anonymous genes were 4.2% and 11.7% and 6.2% and 11.2% in T. laxmanni and T. angustifolia, respectively, based on the BLAST results. The study concluded that they have certain species-specific genes. Hence, the results of this study on these two species could be a valuable resource for further studies.

Fabrication of 3D Multilayered Microfluidic Channel Using Fluorinated Ethylene Propylene Nanoparticle Dispersion (불소화 에틸렌 프로필렌 나노 입자 분산액을 이용한 3차원 다층 미세유체 채널 제작)

  • Min, Kyoung-Ik
    • Korean Chemical Engineering Research
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    • v.59 no.4
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    • pp.639-643
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    • 2021
  • In this study, fluorinated ethylene propylene (FEP) nanoparticle as an adhesive for fabricating a three-dimensional multilayered microfluidic device was studied. The formation of evenly distributed FEP nanoparticles layer with 3 ㎛ in thickness on substrates was achieved by simple spin coating of FEP dispersion solution at 1500 rpm for 30 s. It is confirmed that FEP nanoparticles transformed into a hydrophobic thin film after thermal treatment at 300 ℃ for 1 hour, and fabricated polyimide film-based microfluidic device using FEP nanoparticle was endured pressure up to 2250 psi. Finally, a three-dimensional multilayered microfluidic device composed of 16 microreactors, which are difficult to fabricate with conventional photolithography, was successfully realized by simple one-step alignment of FEP coated nine polyimide films. The developed three-dimensional multilayered microfluidic device has the potential to be a powerful tool such as high-throughput screening, mass production, parallelization, and large-scale microfluidic integration for various applications in chemistry and biology.

Early overcounting in otoliths: a case study of age and growth for gindai (Pristipomoides zonatus) using bomb 14C dating

  • Andrews, Allen H;Scofield, Taylor R.
    • Fisheries and Aquatic Sciences
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    • v.24 no.1
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    • pp.53-62
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    • 2021
  • Gindai (Pristipomoides zonatus) is one of six snappers in a management complex called the Deep 7 of the Hawaiian Islands. Little is known about its life history and a preliminary analysis of otolith thin sections indicated the species may exhibit moderate growth with a lifespan approaching 40 years. Preliminary age estimates from the previous study were reinvestigated using the same otolith sections in an attempt to validate those ages with bomb radiocarbon (14C) dating. From the misalignment of birth years for the otolith 14C measurements with regional references - the post-peak bomb 14C decline period - it was concluded that previous ages were inflated from overcounting of the earliest growth zone structure in otolith sections. The oldest gindai was re-aged to 26 years once the age reading was adjusted for early overcounting, 13 years younger than the original estimate of 39 years for this fish. In general, the earliest otolith growth of gindai was massive and complicated by numerous subannual checks. The approach of lumping the early growth structures was supported by the alignment of 14C measurements from otolith core material (first year of growth). The result was greater consistency of calculated birthdates with the 14C decline reference, along with minor offsets that may indicate age estimation was imprecise by a few years for some individuals. The revised von Bertalanffy growth function applied to the validated age-at-length estimates revealed more rapid growth (k = 0.378 cf. 0.113) and a lifespan of approximately 30 years. The findings presented here are a case study of how the bomb 14C decline period can be used as a tool in the refinement of age reading protocols.

Smart monitoring system using electromagnetic waves to evaluate the integrity of reinforced concrete structural elements

  • Jong-Sub Lee;Dongsoo Lee;Youngdae Kim;Goangseup Zi;Jung-Doung Yu
    • Computers and Concrete
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    • v.31 no.4
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    • pp.293-306
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    • 2023
  • This study proposes and demonstrates a smart monitoring system that uses transmission lines embedded in a reinforced concrete structure to detect the presence of defects through changes in the electromagnetic waves generated and measured by a time-domain reflectometer. Laboratory experiments were first conducted to identify the presence of voids in steel-concrete composite columns. The results indicated that voids in the concrete caused a positive signal reflection, and the amplitude of this signal decreased as the water content of the soil in the void increased. Multiple voids resulted in a decrease in the amplitude of the signal reflected at each void, effectively identifying their presence despite amplitude reduction. Furthermore, the electromagnetic wave velocity increased when voids were present, decreased as the water content of the soil in the voids increased, and increased with the water-cement ratio and curing time. Field experiments were then conducted using bored piles with on-center (sound) and off-center (defective) steel-reinforcement cage alignments. The results indicated that the signal amplitude in the defective pile section, where the off-center cage was poorly covered with concrete, was greater than that in the pile sections where the cage was completely covered with concrete. The crosshole sonic logging results for the same defective bored pile failed to identify an off-center cage alignment defect. Therefore, this study demonstrates that electromagnetic waves can be a useful tool for monitoring the health and integrity of reinforced concrete structures.

Morphometric and genetic diversity of Rasbora several species from farmed and wild stocks

  • Bambang Retnoaji;Boby Muslimin;Arif Wibowo;Ike Trismawanti
    • Fisheries and Aquatic Sciences
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    • v.26 no.9
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    • pp.569-581
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    • 2023
  • The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

Enhancement of BIM Modeling Automation Algorithm for Linear-Based Tunnel Infrastructure and Development of BIM Modeling Automation System (선형기반 터널 인프라 구조물의 BIM 모델링 자동화 알고리즘 개선 및 BIM 모델링 자동화 시스템 개발)

  • Kim, Yun-Ok;Kim, Ji-Young; Kim, Tae-Min;Moon, So-Yeong
    • Journal of KIBIM
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    • v.13 no.3
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    • pp.1-11
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    • 2023
  • In order to use BIM as a tool for improving the productivity and quality of products in the construction industry, a BIM model must be created from the design stage first. Infrastructure structures such as bridges and tunnels are mainly created based on three-dimensional alignment in the generation of BIM models. Especially, generation of BIM models based on three-dimensional linearity has high task difficulty and algorithms for automating BIM modeling for railway infra structures have been suggested in previous studies. This study improved the BIM modeling automation algorithm of railway infrastructures and developed a system based on the algorithm so that it can be easily used by ordinary users. The system was built as an add-in system of Autodesk's Revit. As an improvement first, it is possible to arrange different libraries for each pattern, enabling various uses. In addition, it can be created models of several members with a single process and the system can automatically places structures that are added periodically, such as Rock Bolt and Fore Polling. Finally, 3D length information and volume for each pattern are automatically calculated for more accurate 3D-based volume calculation. This study contributes to increasing user accessibility by building a BIM modeling automation algorithm into a system. The system is expected to improve the efficiency of BIM modeling creation of linear-based infra structures, including railway infrastructure.

Molecular identification of fruit bats, natural host of Nipah virus in Bangladesh, based on DNA barcode

  • Md. Maharub Hossain Fahim;Walid Hassan;Afia Afsin;Md. Mahfuzur Rahman;Md. Tanvir Rahman;Sang Jin Lim;Yeonsu Oh;Yung Chul Park;Hossain Md. Faruquee;Md. Mafizur Rahman
    • Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.163-172
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    • 2024
  • Background: Fruit bats are natural carriers of Nipah virus (NiV). The primary objective of this study is to identify potential reservoir species in a selected geographic regions. It is necessary to determine an accurate species identification of the associated reservoir bat species distributed in a specific region. Results: In this study, we collected 20 different bat specimens from the NiV-prone area of the Kushtia district. Among these, 14 were tissue samples (BT-1-14) and six were fecal samples (BF-1-6). We used the mitochondrial gene cytochrome b, one of the most abundant and frequently used genetic markers, for polymerase chain reaction amplification and sequencing. Out of the 20 samples, 12 tissue samples and 2 fecal samples were successfully amplified and sequenced. However, two tissue samples and four fecal samples yielded chimeric sequences, rendering them unsuitable for annotation. The sequences of the successfully amplified samples were compared to those deposited in the National Center for Biotechnology Information database using basic local alignment search tool to identify the bat specimen collected. The study identified six different bat species using both morphological and genetic data, which may carriers of the NiV. Conclusions: Our results suggest that additional research should be conducted to gather more information on fruit bats from different localities across the country. The study contributes to the establishment of appropriate measures for NiV carrying disease control and management.

Study on MPI-based parallel sequence similarity search in the LINUX cluster (클러스터 환경에서의 MPI 기반 병렬 서열 유사성 검색에 관한 연구)

  • Hong, Chang-Bum;Cha, Jeoung-Ho;Lee, Sung-Hoon;Shin, Seung-Woo;Park, Keun-Joon;Park, Keun-Young
    • Journal of the Korea Society of Computer and Information
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    • v.11 no.6 s.44
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    • pp.69-78
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    • 2006
  • In the field of the bioinformatics, it plays an important role in predicting functional information or structure information to search similar sequence in biological DB. Biolrgical sequences have been increased dramatically since Human Genome Project. At this point, because the searching speed for the similar sequence is highly regarded as the important factor for predicting function or structure, the SMP(Sysmmetric Multi-Processors) computer or cluster is being used in order to improve the performance of searching time. As the method to improve the searching time of BLAST(Basic Local Alighment Search Tool) being used for the similarity sequence search, We suggest the nBLAST algorithm performing on the cluster environment in this paper. As the nBLAST uses the MPI(Message Passing Interface), the parallel library without modifying the existing BLAST source code, to distribute the query to each node and make it performed in parallel, it is possible to easily make BLAST parallel without complicated procedures such as the configuration. In addition, with the experiment performing the nBLAST in the 28 nodes of LINUX cluster, the enhanced performance according to the increase in the number of the nodes has been confirmed.

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Manufacture of 3-Dimensional Image and Virtual Dissection Program of the Human Brain (사람 뇌의 3차원 영상과 가상해부 풀그림 만들기)

  • Chung, M.S.;Lee, J.M.;Park, S.K.;Kim, M.K.
    • Proceedings of the KOSOMBE Conference
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    • v.1998 no.11
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    • pp.57-59
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    • 1998
  • For medical students and doctors, knowledge of the three-dimensional (3D) structure of brain is very important in diagnosis and treatment of brain diseases. Two-dimensional (2D) tools (ex: anatomy book) or traditional 3D tools (ex: plastic model) are not sufficient to understand the complex structures of the brain. However, it is not always guaranteed to dissect the brain of cadaver when it is necessary. To overcome this problem, the virtual dissection programs of the brain have been developed. However, most programs include only 2D images that do not permit free dissection and free rotation. Many programs are made of radiographs that are not as realistic as sectioned cadaver because radiographs do not reveal true color and have limited resolution. It is also necessary to make the virtual dissection programs of each race and ethnic group. We attempted to make a virtual dissection program using a 3D image of the brain from a Korean cadaver. The purpose of this study is to present an educational tool for those interested in the anatomy of the brain. The procedures to make this program were as follows. A brain extracted from a 58-years old male Korean cadaver was embedded with gelatin solution, and serially sectioned into 1.4 mm-thickness using a meat slicer. 130 sectioned specimens were inputted to the computer using a scanner ($420\times456$ resolution, true color), and the 2D images were aligned on the alignment program composed using IDL language. Outlines of the brain components (cerebrum, cerebellum, brain stem, lentiform nucleus, caudate nucleus, thalamus, optic nerve, fornix, cerebral artery, and ventricle) were manually drawn from the 2D images on the CorelDRAW program. Multimedia data, including text and voice comments, were inputted to help the user to learn about the brain components. 3D images of the brain were reconstructed through the volume-based rendering of the 2D images. Using the 3D image of the brain as the main feature, virtual dissection program was composed using IDL language. Various dissection functions, such as dissecting 3D image of the brain at free angle to show its plane, presenting multimedia data of brain components, and rotating 3D image of the whole brain or selected brain components at free angle were established. This virtual dissection program is expected to become more advanced, and to be used widely through Internet or CD-title as an educational tool for medical students and doctors.

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