• Journal of Life Science
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• v.30 no.9
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• pp.798-803
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• 2020

Late in the 1960s, South Korea established cultivation technology and began breeding new "Tongil" type rice cultivars by crossing indica and japonica. To date, this cultivation technique has been used to produce a wide variety of cultivars to meet consumer preferences and adapt to extreme weather conditions. Once major genetic traits are no longer segregated through advanced generations, varieties obtained from a cross between different crops may become a new variety. In this study, we confirmed the agronomic traits of F₄, F₅, and F₆ by advanced generation a population in which drought tolerance and brown planthopper (BPH) resistance were crossed. HV23, Ilmi/HV23, and Drimi2ho/HV23 were used. HV23 was drought-tolerant, and Drimi2ho was resistant to BPH. As a result, it was possible to consider that the agronomic traits were fixed because none of them showed a significant difference from the others. While it takes more than 10 years for the pedigree method of breeding, this study confirmed that the agronomic traits were fixed in 4-6 years. In the future, we will investigate the homology of the CaMsrB2 gene and the Bph1 gene, to confirm that both genes are closely related to each other, and analyze the stable inheritance of the introduced gene for multiple successive generations.

• Korean Journal of Environmental Agriculture
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• v.14 no.1
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• pp.1-6
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• 1995

This experiment was carried out to clarify the evapotranspiration and water use efficiency in rice plant canopy. Two rice cultivars, Daechungbyo(japonica type) and Samgangbyo(Tongil) were planted on the field of Suwon Weather Forecast Station in 1989. Evapotranspiration, dry matter production and leaf area of rice plant were measured to investigate the water use efficiency. There was significant correlation between cumulative evapotranspiration and dry matter production of aboveground. The parameter of linear regression was 4.13. The ratio of cumulative top dry matter production per cumulative evapotranspiration was increased until $5.5{\sim}5.9$ leaf area index. The de Wit's "m" value revealed maximum record at heading-flowering stage. At the harvest, the values were ranged from 175.5 to 191.7. The parameter of cumulative solar radiation to dry matter production was $1.011{\sim}1.248$. The evapotranspiration ratio(g.water/g.DW) of Samgangbyo(278) was higher than that of Daechungbyo (299.9). The efficiency of evapotranspiration(g.DW/g. water) was 1.58 in Daechungbyo and 1.98 in Samgangbyo.

• Current Research on Agriculture and Life Sciences
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• v.27
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• pp.21-28
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• 2009

The purpose of this study was to improve the culturability of 'IR 36', a indica type rice cultivar using DNA marker associated with the ability of plant regeneration in anther and seed culture. The culturability of 6 rice cultivars and 2 indica/japonica lines ('MGRI 036', 'MGRI 079') were investigated in anther and seed culture. The culturability of 3 japonica rice cultivars were much higher than tongil and indica rice cultivars, and 'MGRI 036' and 'MGRI 079' has high culturability with 20% regenerability, also. 34 $BC_2F_4$ 4 lines were selected by marker screening using RZ400 among 90 $BC_2F_4$ lines derived from a cross between 'MGRI 079' and 'IR 36'. The frequency of callus formation of 10 $BC_2F_4$ lines were higher than 'IR 36' in anther culture among selected 34 $BC_2F_4$ lines. The ability of plant regeneration of 10 lines were higher than 'IR 36' in the seed culture among selected 34 $BC_2F_4$ lines. A promising line, $BC_2F_4$-28, was selected to have better culturability in the anther and seed culture among selected 34 $BC_2F_4$ lines. The heading date and grain shape of the $BC_2F_4$-28 was similar to 'IR 36'. Using the RZ400 DNA marker associated with the culturability will be useful method for improving of indica rice culticvar's culturability in rice breeding program.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.3
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• pp.205-211
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• 2005

This study was carried out to construct cold-tolerance characteristics. The RILs were developed from progenies of a cross between cold-susceptible Tongil-type rice variety, Milyang 23 and cold-tolerant Japonica rice variety, Stejaree 45 by single seed descent methods. The 175 RILs $(F_8)$ were evaluated for cold tolerance traits by field screening under cold-water irrigation. Frequency distribution of RILs in leaf discoloration, heading delay, culm length reduction and number of spikelets reduction displayed nearly normal distributions with transgressive segregations to either side of parents, while the spikelet fertility reduction and panicle exsertion at low-temperature showed the more or less skewed continuous distribution toward the susceptible parent. Higher heritabilities over $60\%$ were observed in leaf discoloration, spikelet fertility reduction, panicle exsertion, while relatively lower heritabilities less than $40\%$ were observed in culm length reduction, number of spikelets reduction and grain yield reduction. Some cold-tolerance RILs were selected effectively by cold water irrigation, which are expected to be good materials in breeding program for cold tolerance.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.21-28
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• 1991

The effect of subculture intervals and passages on plant regeneration from seed-derived callus was determined. Regeneration capacity of callus varied with rice cultivars and subculture intervars tested. The callus subcultured every 2 weeks produced more plants than that of 4 weeks. The calli from a Tongil-type rice cultivar, Milyang 23, lost easily their regeneration ability when the calli were subcultured every 2 weeks and 4 weeks. The callus induced from a japonica cultivar, "Yeongdeogbyeo", showed to maintain high frequency(>70%) of plant regeneration when it was subcultured every 2-week intervals. Casein hydrolysate supplemented in callus induction medium enhanced callus growth and its regeneration. High frequency of plant regeneration was obtained from the calli transferred on $N_6$ medium supplemented with kinetin(2mg/1) and NAA(1mg/1). The subcultured calli in the medium supplemented with casein hydrolysate(2 g/1), myo-inositol(200mg/1) and thiamine-HCl(2mg/1) increased the frequency of embryogenic callus formation and plant regeneration.

• Korean Journal of Soil Science and Fertilizer
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• v.9 no.3
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• pp.183-199
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• 1976

1. The cultivated land in Korea has originally low fertility resulting in high dependence to fertilizers. 2. The total fertilizer requirement calculated by the Office of Rural Development (ORD) in 1976 was about 1,153 thousand mts, and the total amount of supply planned by the Ministry of Agriculture and Fishery was 1,010 thoushand mts which is close to the amount calculated by ORD. However, there is some gap between the amount of recommended (N, 11.6; $P_2O_5$: $K_2O$, 7.3kg/10a> and supply planned (N, 12.0; $P_2O_5$, 6.3; $K_2O$. 4.8kg/10a) fertilizers for each elements per unit area 3. For 15 years from 1960 to 1975 the fertilizer consumption of nitrogen was roughly increased from 200,000 mts to 500,000 mts; phosphorus, from 50,000 to 250,000 mts; potassium, from 10, 000 to 170,000 mts; accounting 2.5, 5, and 17 times of increase respectively. 4. The total fertilizer consumption for 5 years from 1967 to 1971 was about 100,000 mts and another 5years from 1971 to 1975 was 300,000 mts indicating three times increase. 5. The direct factors influenced to the increase of fertilizer consumption in recent years are 1) the dissemination of high yielding Tongil type rice varieties which are resistant to heavy fertilization 2) high price policy for agricaltural products 3) increased cultivation of vegetables:, fruits, and forages which require high level of fertilizers. The indirect factors are 1) dissemination of new improved agricultural techniques, 2, improvement of cultivated land conditions through irrigation system and land reform, 3) increased supply of silicate fertilizers, and 4) increase of farm income. 6. The percentage of total fertilizer consumption by rice (32%) and barley (25%) is about 57%. The ratio of total fertilizer consumption by vegetables and forages is expected to increase greatly. 7. Based on the increasing tendency of cultivated land and yield per unit area for last 10 years in each crop, total fertilizer consumptions in 1980, 1990, and 2000 year are estimated to 1,290,000, 1,580,000 and 1,870,000 mts respectively.

• Korean Journal of Agricultural Science
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• v.5 no.2
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• pp.115-119
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• 1978

To improve the feed value of rice straw, Tongil type rice straw and Persicaria Thunbergii Gross var. coreana Gross ex Nakai were mixed in the ratios of 10 : 90 (T1), 20 : 80 (T2), 30 : 70 (T3) and 40 : 60 (T4) in fresh weight and ensiled for 6 weeks. The chemical composition of fresh materials and silage, the organic acid contents and pH in silage were determined. And the results obtained were summarized as follow: 1. The crude protein content in rice straw was 8.18% of DM basis and that in Persicaria Thunbergii was 14.47%. And the nitrogen-free extract content in rice straw was 44.66% and.. that in Persicaria Thunbergii was 51.13%. 2. The optimum moisture contents were appeared in T2 as 72.12% and T3 as 66.43%. Considerably high crude protein contents were determined from all of treatments. 3. The pH of silage was varied from pH 3.8 of T1 to pH 4.5 of T2. And Total organic acid contents were increased from T1 to T4, but the percentage of lactic acid contents to total acid contents were decreased. 4. It was concluded that good quality silage could be made from rice straw with Persicaria Thunbergii and the best ratios of rice straw: Persicaria Thunbergii were between 20 : 80 and 30 : 70 in fresh weight.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.300-300
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• 2017

'Saemimyeon' a Tongil-type rice variety (Indica ${\times}$ Japonica), which contains high amylose contents is suitable for rice noodle production. Nowadays, the major parts of rice processing industry that includes products like rice flour and noodles are expected to partially replace wheat flour market. The volume of rice noodle market is getting bigger and can contribute to the rice surplus and farmer's income. This study was carried out to promote productivity and flour-making quality of 'Saemimyeon' by finding the most suitable transplanting and harvesting times. The transplanting days used were May $10^{th}$, May $17^{th}$, May $24^{th}$, May $31^{th}$, June $7^{th}$ and June $14^{th}$ and the planting distance used was 30 x 12cm. In addition, harvesting time was determined by days after heading time (40, 45, 50, 55 and 60 days). The field experiment was conducted at the experimental field in Miryang (Southern plain area of Korea) from 2015 to 2016. Our results suggest that the optimum transplanting days were from May $24^{th}$ to May $31^{th}$ which resulted to an average yield of 748~751kg/10a. Interestingly, yield was sharply decreased below 700kg/10a before May $10^{th}$ and after June $7^{th}$. The average grain filling rate before May $31^{th}$ was more than 83% but it declined to 75% after June $7^{th}$. The average temperature ranges from heading time to harvesting time was $ 21\sim25^{\circ}C$ and the estimated optimal temperature was $23.4^{\circ}C$ which is similar to May $24^{th}$ by regression equation. We found that the optimal harvest time was 45~50 days after heading time. It is hypothesized that low temperature at seed maturation time caused the lower grain filling rate therefore 'Saemimyeon' need to be transplanted before May $31^{th}$ for higher productivity. We found no statistical variation in amylose contents among experimental plots (28.2~30.4%). We conclude that the productivity of 'Saemimyeon' highly depends on temperature that is critical for grain filling stage controlled by transplanting time.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.67-67
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• 2017

We developed the breeding materials with diverse grain size and shape in japonica rice. Grain size and shape are important factors affecting consumer preference and choice. However, most of Korean japonica rice cultivars have small, short, and round grain. To diversify the grain size and shape of japonica rice, we conducted the breeding program using donor parents, Jizi1560 and Jizi1581. Jizi1560 and Jizi1581 are japonica germplasm with extremely large grain. Four crosses between the each donor parents and high yielding japonica rice cultivars, Deuraechan and Boramchan, were constructed and then anther culture method was applied. We obtained 290 doubled-haploid (DH) lines with appropriated morphological traits and selected 91 DH lines with diverse grain size and shape. The grain related-traits of the selected DH lines showed a higher diversity when compared with 319 cultivars developed by NICS (264 japonica, 13 black, and 32 Tongil type cultivars). We designated the selected DH lines, four parents, and Daeripbyeo 1, large grain japonica cultivar, as the breeding materials for further analysis. The breeding materials were classified into five groups, A to E, based on the grain-related traits. Group A (including Jizi1581) and Group B (including Daeripbyeo 1) showed similar grain width, whereas Group A exhibited longer grain length and heavier grain weight. Group C (including Deuraechan and Boramchan) showed shorter and rounder grain shape and smaller grain size than any other groups. Group D including solely Jizi1560 had extremely large grain, such as the longest grain length, width, and thickness and heaviest grain weight. Group E including only two DH lines had long and slender grain shape, so that showed the highest ratio of length to width. The grain size and shape of the breeding materials exhibited beyond the characteristics of previously developed Korean japonica cultivars. The breeding materials will be applied in the breeding programs to diversify the grain size and shape of japonica rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.1
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• pp.12-40
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• 1983

Heading behaviour of native japonica (GI) and Japanese varieties (GII) grown during 1910-1920s were thermo-sensitive, home bred varieties during 1930s (GIII) - 1950s (GIV) were photo-sensitive and semi-dwarf indica derived varieties released since 1971 (GV) were controlled by basic vegetative phase. Number of leaves on main culm was increased with varietal improvement and culm length was gradually shortened as variety improved. GV variety had the shortest plant height among the groups. The first internode length was about 30cm in all groups and difference of 2nd-4th internode length was caused shortening the culm length. Panicle length and diameter of panicle neck were shortened and became thinner as variety developed from GI to GIV, however, GV had longer panicle and thick panicle neck, respectively. Number of panicles/hill and straw weight/hill increased and became heavier as variety improve from GI to GIV but GV had moderate number of panicle/hill and relatively light straw weight. Number of spikelet/panicle has been reduced with varietal improvement but GV had the biggest number. Grain yield/hill also increased as variety improved, however, it was decreased as transplanting season postponed and GV had shown the most sensitive difference. Top dry matter weight at heading stage and at 20 days after heading (20 DAH) had no difference among the groups except GI which was the smallest. Average grain weight at 20 DAH was heavier in the order of GI > GII, GV > GIV > GIII. It had a little variation in GI & GII while that of other variety group showed significant decrease as transplanting delayed. Harvest index (HI) of GV was the highest at 54% for the earlier transplanting and GIII had the lowest HI. Difference of HI became conspicuous for the later transplanting, the varieties bred later had the lower HI when transplanting of rice delayed. At the earliest transplanting applied (May 15), the grain yield was higher in the order of GI > GIV > GIII > GII > GI, however, that of Tongil type variety (GV) was the lowest for the latest transplanting (June 29). Interrelationship among the agronomical characteristics and grain yield was discussed.