• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.289-289
• /
• 2022

Waterlogging tolerance of corn is one of the important factor for cultivate in paddy soil condition to increase cultivation area and self-sufficiency of corn in Korea. In order to develop elite waterlogging tolerance corn, the new corn lines bred by crossing wild corn, Teosinte, and cultivated corn inbred lines. Five accessions among the 2 species, Zea mays sub spp. mexicana and Zea mays spp. parviglumis, of 81 Teosinte were selected through the waterlogging treatment. The waterlogging treatments were implemented for 7 days at the seedling(V3) stage. The inbred lines were developed by crossing 5 teosinte accessions and cultivated corn lines and they were estimated waterlogging tolerance. It was screened and analyzed the metabolites extracted from roots of 19KT-32(KS141 × teosinte) that was treated waterlogging. We selected 8 of 180 metabolites like as γ-aminobutyric acid(GABA), putrescine, citrulline, Gly, and Ala that expression was remarkably changed over 2.5-times, 7 metabolites increased and 1 metabolite decreased in waterlogging, respectively. Glutamate decarboxylase(GAD) catalyzing GABA accumulation gene have 10 haplotypes, and exon1 was highly conserved, but identified to 135 SNPs after the first intron. Among the 135 SNPs, the number of transversion mutations (52) surpassed the number of transition mutations (38). Most of metabolites were related to abiotic stress in plant that it regulated to pH, osmotic pressure K⁺/Ca⁺⁺ and ATPase activity. We are analyzing the association using these results for increase breeding efficiency.

• 한국산림과학회지
• /
• 제90권1호
• /
• pp.83-89
• /
• 2001

본 연구는 외생균근균 중 중금속에 대한 내성이 우수한 것으로 알려져 있는 모래밭버섯균의 Cd 축적과 내성 특성을 밝히고자 수행하였다. 모래밭버섯균은 1997년 전남 무안의 곰솔림에서 분리한 것으로 MMN 배지에 $CdSO_4{\cdot}5H_2O$을 0, 0.2, 0.5, 2, $10{\mu}g/m{\ell}$로 첨가하여 기내에서 배양하였다. 배양을 시작한 후 일주일 간격으로 24일 동안 생장량과 내성지수를 측정하였으며, 40일 후에는 균사를 수확하여 Cd 함량, superoxide dismutase(SOD) 활성, glutathione reductase(GR) 활성을 측정 하였다. 기내 배양된 모래밭버섯균은 Cd $2{\mu}g/m{\ell}$ 처리에서 생장이 감소하였으며, Cd $10{\mu}g/m{\ell}$의 처리에서는 생장이 거의 정지하였다. 대조구와 Cd 처리구의 생장량 비로 결정된 모래밭버섯균의 내성지수는 Cd 처리 농도가 증가함에 따라 점차 감소하는 경향을 나타냈으나 Cd $0.5{\mu}g/m{\ell}$와 Cd $2{\mu}g/m{\ell}$ 처리구에서는 14일 경과 후 다시 증가하였다. 모래밭버섯균의 균사내 Cd 농도는 Cd 처리 농도 증가에 따라 증가하였으며, 특히 Cd $10{\mu}g/m{\ell}$ 처리의 균사내 Cd 농도는 $854{\mu}g/g$으로, Cd $0.5{\mu}g/m{\ell}$ 처리구의 균사내 농도 $12.3{\mu}g/g$보다 70배 높았다. 모래밭버섯균의 SOD 활성은 Cd 농도별 처리간에 큰 차이를 보이지 않았다. 그러나 GR 활성은 Cd $0.5{\mu}g/m{\ell}$ 처리구에서 대조구보다 높았으며 Cd $2{\mu}g/m{\ell}$에서는 대조구보다 낮았다. 결론적으로 모래밭버섯균은 Cd의 흡수가 뛰어나며, Cd에 대한 내성은 비교적 저농도에서 항산화효소의 합성 증가와 같은 독성 제거 기작을 통하여 나타난다고 판단된다. 따라서 모래밭버섯균은 저농도로 오염된 중금속 오염지의 식생 복원에 이용이 가능하다고 본다.

• Journal of Nutrition and Health
• /
• 제26권3호
• /
• pp.299-312
• /
• 1993

Anthropometry, computed tomography(CT) at the umbilical level, nutrient intake, blood pressure, serum levels of lipids and lipoproteins and response of glucose, c-peptide, insulin, and free fatty acid(FFA) during oral glucose tolerance test(OGTT) were estimated on 11 normal-weight controls and 35 overweight and obese middle-agd men. The areas of total abdominal, subcutaneous and visceral were determined by CT scanning technique. Total abdominal fat area correlated the most significantly with the levels of serum lipids, lipoproteins and insulin among several obese indices. Compared with normal-weight controls, overweight and obese men with abdominal fat lower than 29000$\textrm{mm}^2$ showed an increase in waist-hip ratio, areas of total abdominal(35%), visceral and subcutaneous fat and C-peptide response area during OGTT, though age, percent ideal body weight, body mass index, % body fat, and all biochemical indices except C-peptide response area were not different between two groups. Overweight and obese men with abdominal fat greater than 29000$\textrm{mm}^2$ showed a higher values in total abdominal fat(85%), serum levels of triglyceride, total-and LDL-cholesterol, the ratio of LDL-to HDL-cholesterol, and response areas of FFA, insulin and C-peptide during OGTT than normal-weight controls. Overweight and obese men with great abdominal fat showed an increase in alcohol ingestion and percent calorie intake per total energy expenditure, compared with normal-weight controls. Our results indicate that obesity and a certain level of total abdominal fat accumulation is required to observe abnormal levels of serum lipids, lipoproteins and insulin in Korean middle-aged men. In addition, increased alcohol and calorie intake and decreased physical activity could partly explain total abdominal fat accumulation in men.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.38-38
• /
• 2017

Water stress obstructs rice growth mainly by oxidative damage in biological cells to cause a reduction of leaf photosynthesis and evapotranspiration processes. In this study, exogenous application of vanillic acid (VA) and p-hydroxybenzoic acid (PHBA) to improve drought tolerance of two Oryza sativa cultivars, Q2 and Q8 was tested. The drought evaluation based on leaf phenotypes to show that both Q2 and Q8 resulted in remarkable water-stress tolerance induced by leaf spraying pretreatment of mixed solution of $50{\mu}M\;VA+50{\mu}M\;PHBA$. The mixtures of $25{\mu}M\;VA+25{\mu}M\;PHBA$ and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treated on Q2 and Q8 in water deficit condition also indicated that total phenols, total flavonoids, and DPPH radical scavenging activity were significantly greater their controls. In general, the accumulation of individual phenolic acids was increased in exogenous phenolic treatments, as compared with controls. Particularly, Q2 obtained a considerable amount of endogenous PHBA after application of $50{\mu}M\;VA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ (0.18 mg/g DW, 0.71 mg/g DW, and 1.41 mg/g DW, respectively); and a negligible content of VA (0.003 mg/g DW) appeared uniquely in the treatment of $50{\mu}M\;VA$. Similarly, Q8 also absorbed a significant quantity of PHBA in $50{\mu}M\;PHBA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treatments (0.15 mg/g DW, 0.15 mg/g DW, and 0.22 mg/g DW, respectively). In addition, the spraying $50{\mu}M\;PHBA$ and $25{\mu}M\;VA+25{\mu}M\;PHBA$ on Q8 leaves induced similar amount of drought tolerance of Q2 and Q8 were improved, paralleled with the increased amounts of endogenous phenolics revealed that VA and PHBA played an important role to enhance drought tolerance in rice.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.33-33
• /
• 2017

Water stress obstructs rice growth mainly by oxidative damage in biological cells to cause a reduction of leaf photosynthesis and evapotranspiration processes. In this study, exogenous application of vanillic acid (VA) and p-hydroxybenzoic acid (PHBA) to improve drought tolerance of two Oryza sativa cultivars, Q2 and Q8 was tested. The drought evaluation based on leaf phenotypes to show that both Q2 and Q8 resulted in remarkable water-stress tolerance induced by leaf spraying pretreatment of mixed solution of $50{\mu}M\;VA+50{\mu}M\;PHBA$. The mixtures of $25{\mu}M\;VA+25{\mu}M\;PHBA$ and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treated on Q2 and Q8 in water deficit condition also indicated that total phenols, total flavonoids, and DPPH radical scavenging activity were significantly greater their controls. In general, the accumulation of individual phenolic acids was increased in exogenous phenolic treatments, as compared with controls. Particularly, Q2 obtained a considerable amount of endogenous PHBA after application of $50{\mu}M\;VA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ (0.18 mg/g DW, 0.71 mg/g DW, and 1.41 mg/g DW, respectively); and a negligible content of VA (0.003 mg/g DW) appeared uniquely in the treatment of $50{\mu}M\;VA$. Similarly, Q8 also absorbed a significant quantity of PHBA in $50{\mu}M\;PHBA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treatments (0.15 mg/g DW, 0.15 mg/g DW, and 0.22 mg/g DW, respectively). In addition, the spraying $50{\mu}M\;PHBA$ and $25{\mu}M\;VA+25{\mu}M\;PHBA$ on Q8 leaves induced similar amount of VA (0.04 mg/g DW). Meanwhile, there were no trace of VA and PHBA found in controls. The levels of drought tolerance of Q2 and Q8 were improved, paralleled with the increased amounts of endogenous phenolics revealed that VA and PHBA played an important role to enhance drought tolerance in rice.

• 제어로봇시스템학회논문지
• /
• 제17권11호
• /
• pp.1101-1105
• /
• 2011

Radiation particles can introduce temporary errors in memory systems. To protect against these errors, so-called soft errors, error detection and correcting codes are used. In addition, scrubbing is applied which is a fundamental technique to avoid the accumulation of soft errors. This paper introduces an optimal scrubbing scheme, which is suitable for a system with auto error detection and correction logic. An auto error detection and correction logic can correct soft errors without CPU's writing operation. The proposed scrubbing scheme leads to maximum reliability by considering both allowable scrubbing load and the periodic accesses to memory by the tasks running in the system.

• 한국유기농업학회지
• /
• 제24권2호
• /
• pp.273-287
• /
• 2016

Accumulation of excessive salt in Reclaimed coastal tidelands can reduce crop yields, reduce the effectiveness of irrigation, degradation of soil structure, and affect other soil properties. These salts has shown to cause specific ions in the plant over a period of time leads to ion toxicity or ion imbalance and a continuous osmotic phase that prevents water uptake by plants due to osmotic pressure of saline soil solution. This review focuses on the characteristics of salt-affected soils, mechanisms of salt-tolerance plants, desalinization technology, and soil management to maintain sustainable agro-ecosystem in salt-affected soils.

• 한국생명과학회:학술대회논문집
• /
• 한국생명과학회 2003년도 제39회 학술심포지움
• /
• pp.136-136
• /
• 2003

• 대한기계학회논문집
• /
• 제14권5호
• /
• pp.1181-1185
• /
• 1990

본 연구에서는 정적파괴는 피로균열 진전의 최종과정에서 생기는 경우가 많다. 따라서 정적파괴의 경우의 파괴평가선도 및 피로파괴의 경우의 파괴평가선도가 얻어지 면 실제의 사용에 대해서 대단히 유용하다. 이와같은 관점에서 피로파괴에 대한 파 괴평가선도와 데이터베이스(data base)의 구축을 검토해 보고자 한다.

• KSBB Journal
• /
• 제21권2호
• /
• pp.79-84
• /
• 2006

본 연구에서는 Corynebacterium glutamicum I 균주에 salt tolerance를 도입하여 L-lysine 생산량을 증가시키고자 하였다. I 균주를 이용하여 mutagenesis를 수행한 후 모균주가 생장하는 못하는 9%의 NaCl이 포함된 배지에서 빠르게 생장하는 C14-49-3-15-7-3-20 균주를 선별하였다. flask 배양으로 L-lysine 생산을 조사한 결과 모균주 I의 경우 L-lysine 농도가 53.3 g/L, 수율이 51.6%인 반면 변이주 C14-49-3-15-7-3-20의 경우에는 L-lysine 농도 61.2 g/L, 수율 61.0%로 나타났다. 그리고 5 L 발효조에서 유가식 배양법으로 배양하여 L-lysine 생산량을 조사하였다. 그 결과 모균주는 113.0 g/L의 L-lysine을 생산하였고 수율은 41.8%이었다. 하지만 변이주의 경우에는 $33^{\circ}C$로 유지하여 배양한 후 PCV가 7.5%가 되는 시점에 배양 온도를 $35^{\circ}C$로 올려주고 배양하였을 때 L-lysine 생산량이 130.6 g/L, 수율이 48.6%로 모균주보다 많은 양의 L-lysine을 생산하였다. L-lysine 생산과 균주의 생장에 대한 osmotic pressure의 영향을 조사하기 위해 변이주 C14-49-3-15-7-3-20을 고농도의 NaCl과 당이 포함되어 있는 배지에 각각 배양하여 균체 생장 및 L-lysine 생산량을 조사하였다. 그 결과 모균주는 균체 생장이 느리고 생산량도 낮은 반면 변이주 C14-49-3-15-7-3-20의 경우에는 균체 생장 정도가 높고 생산량도 모균주보다 높았다. 그리고 2%의 NaCl이 포함되어 있는 배지에 osmoprotectant 를 첨가하였을 경우 모균주는 균체 생장 및 L-lysine 생산량이 높아졌다. 하지만 C14-49-3-15-7-3-20 균주의 경우에는 proline의 영향을 받지 않았다. 이러한 결과로 Corynebacterium glutamicum 균주에 salt tolerance를 도입하면 L-lysine 생산성을 크게 향상시킬 수 있음을 확인하였다.