• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.278-278
• /
• 2022

Recently, the yield potential of high yield Tongil-type rice varieties has greatly increased, reaching 817kg/10a(Geumgang No.1). Moreover, in order to promote rice consumption and strengthen the competitiveness of the rice processing industry, the R&D of high yield Tongil-type rice varieties for each processing purpose, such as rice noodles, grain feed and industrial materials, has been continuously carried out. However, because Tongil-type rice varieties or lines are generally very vulnerable to cold damage, cold tolerance test can be said to be absolutely necessary to improve the cultivation safety. This study is the result of the seedling and field cold tolerance characteristic tests carried out in 2021 of high yield Tongil-type rice breeding lines. For the cold tolerance characteristic test of seedlings, total 303 high yield rice breeding lines for processing were treated in cold water of 13℃ and irrigation depth of 4cm for 10 days from the third-leaf age, then it was evaluated by comparing the degree of discoloration and withering with the checked varieties(Boramchan, Hanahreum No.2). Also, for the test of field, total 186 high yield rice breeding lines for processing were treated in cold water of 17℃ by keeping pouring day and night from 30 days after transplanting to ripening stage, then it was evaluated by comparing the degree of discoloration, delay of heading, shortening rate of stem length and percent of fertile grain etc. with the checked varieties. And the cold tolerance evaluative criteria were classified as strong(1~3), medium(4~6) and weak(7~9) in overall cold tolerance. (Seedling test) As for the degree of cold tolerance of the check variety, 'Boramchan' and 'Hanahreum No.2' showed a response of 'medium' and 'weak', respectively. However, there was no 'strong' line in the high yield rice breeding lines, 2 lines showed a 'medium' response, and 301 lines showed a 'weak' response. Therefore, except for a few lines(0.7%), most lines(99.3%) showed a 'weak' response. (Field test) In terms of the overall cold tolerance of the check variety, both 'Boramchan' and 'Hanahreum No.2' showed a 'medium' response. Similarly, there was no 'strong' line in the case of high yield rice breeding lines, 20 lines showed a 'medium' response, and 166 lines showed a 'weak' response. Therefore, except for some(10.8%) lines, most(89.2%) lines showed a 'weak' response. From the above results, we selected about 100 individuals with less seed shattering and degeneration of the ear tip, and with a relatively high percent of fertile grain, and are continuing to select lines with improved cold tolerance in the F4~F5 group in this year. As such, most of the Tongil-type rice varieties have poor cold tolerance and thus have low cultivation safety at low temperatures. However, it is important to select improved lines through generational progress because there are some lines that still have a certain level of cold tolerance among them.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.44 no.2
• /
• pp.176-179
• /
• 1999

Trinexapac-ethyl[ 4-(cyclopropyl- $\alpha$ -hydroxy-methylene)-3,5-dioxocyclohexane carboxylic acid ethylester] is a growth-retardant for plants by inhibiting a key step in biosynthesis of GA. A treatment of trinexapacethyl generally induces a reduction in vegetative growth and also inhibits heading. In addition, the trinexapacethyl was known to enhance the freeze-tolerance in annual bluegrass, however, the mechanism is not known yet. One possible reason for the enhanced freeze-tolerance may be the antifreeze protein known to be accumulated in intercellular space of the leaf during cold acclimation. In order to see the possible in-duction of the synthesis of antifreeze proteins by trinexacpacethyl, the apoplastic proteins extracted from Kentucky bluegrass treated with trinexapacethyl were analyzed by SDS-PAGE and the presence of the antifreeze protein was observed. In addition, western analysis showed the identity of the protein induced by both a cold acclimation and a trinexapacethyl treatment. It appears that an enhanced freeze-tolerance of the turf grass by trinexapacethyl is due to the synthesis and/or accumulation of the antifreeze protein similar to the enhanced freeze tolerance induced by cold acclimation.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.21 no.6
• /
• pp.1003-1009
• /
• 1997

To study the time course of increased cold tolerance, the physiological responses were measured during the course of a 2-yr study (Feb. 1995~Feb. 1996) of four female college students, whose cold tolerance was proved to be increased through wearing cool clothing in daily life from Sep. 1994 to Feb. 1995. To determine their cold tolerance, subjects rested at 25$\pm$1$^{\circ}C$, then were exposed to 15$\pm$1$^{\circ}C$, 50$\pm$5% R.H. for 90min in Feb. 1995 and Feb. 1996. Subjects' rectal temperatures, mean skin temperatures, heat production, shivering onset, thermal sensations, and comfort showed no significant changes, when they were measured in 1995 and 1996. Based on these results, we can safely assume that mild cold acclimatization coming from wearing cool clothing lasts at least oneyear, that is until the following year. One of the possible explanations for this is that the subjects did not increase their clothing thermalresistances after the cold acclimatization.

• The Korean Journal of Community Living Science
• /
• v.18 no.4
• /
• pp.599-607
• /
• 2007

We analyzed the effects of regular Jjimjilbang (Korean sauna) exposure on the cold tolerance in young and elderly females. Subjects were young (n=7) and elderly (n=7) females who had never had Jjimjilbang exposure experience. The Jjimjil training group was exposured in the Jjimjilbang 19 times per week. Jjimjibang exposure was limited to three times per day. Jjimjil activity was taken freely. To prove the effects of the cold tolerance, subjects were exposed to cold air ($12{\pm}1^{\circ}C,\;60{\pm}10%$RH). In these conditions, subjects were exposed for 30 minutes at which time rectal temperature, skin temperatures, clothing microclimate, energy expenditure, blood pressure and subjective sensation were measured. To prove the effects of the local cold tolerance, vascular hunting reaction was observed measuring the finger skin temperature while the left middle finger tip was immersed in cold water of $0^{\circ}C$ for 30 minutes. The results are as follows. According to repeated Jjimjil exposure, rectal temperature changes, peripheral temperature and energy expenditure were increased gradually in the cold climate chamber. Blood pressure, subjective sensation and vascular hunting reactions did not show any significant difference. In conclusion, regular Jjimjilbang exposure has negative effects on the ability of the body to improve its regulation of temperature especially in cold tolerance.

• Horticultural Science & Technology
• /
• v.32 no.5
• /
• pp.607-613
• /
• 2014

Calcium has been associated with improved cold tolerance in many crops. The aim of this study was to investigate the changes in leaf cell $Ca^{2+}$ distribution and cell organelle ultrastructure of loquat (Eriobotrya japonica Lindl.) plants in response to cold stress at $-3^{\circ}C$, using transmission electron microscopy (TEM). Two loquat accessions, Zaozhong 6 (a commercial cultivar) and oakleaf loquat (a wild relative) were used. Cold tolerance, as measured by leaf browning rate, was higher in oakleaf plants, and calcium treatment improved cold tolerance in both species. Cold stress first induced inward transport of $Ca^{2+}$ from the intracellular space. Then, the imported $Ca^{2+}$ was aggregated around the chloroplast membrane, finally entering the chloroplast. This pattern of $Ca^{2+}$ distribution in leaf cells occurred earlier in Zaozhong 6 than in the wild loquat. With increasing time of cold exposure, the chloroplast membranes of Zaozhong 6 leaves were damaged, blurred and even disappeared, while those of wild oakleaf loquat leaves maintained their structure longer. In Zaozhong 6, cold stress induced a clear cavity between poorly structured granal thylakoids and vesicles appearing inside the chloroplast, while in oakleaf leaves cold stress had little effect on the ultrastructure of chloroplasts (although chloroplast membranes looked blurred). Loquat leaves accumulated free calcium ions around chloroplasts in response to cold stress, with earlier calcium accumulation occurring in the cold-sensitive cultivar Zaozhong 6 than in wild oakleaf loquat. These results demonstrate that these two loquat species have differences in both cold tolerance and calcium accumulation dynamics.

• ALGAE
• /
• v.25 no.2
• /
• pp.99-104
• /
• 2010

A psychrotolerant cyanobacterium, Nodularia spumigena KNUA005, was isolated from a cyanobacterial bloom sample collected near Dasan Station in Ny-${\AA}lesund$, Svalbard Islands during the Arctic summer season. To generate an axenic culture, the isolate was subjected to three purification steps: centrifugation, antibiotic treatment and streaking. The broad antibacterial spectrum of imipenem killed a wide range of heterotrophic bacteria, while the cyanobacterium was capable of enduring both antibiotics, the remaining contaminants that survived after treatment with imipenem were eliminated by the application of an aminoglycoside antibiotic, kanamycin. Physical separation by centrifugation and streaking techniques also aided axenic culture production. According to the cold-tolerance test, this mat-forming cyanobacterium was able to proliferate at low temperatures ranging between 15 and $20^{\circ}C$ which indicates the presence of cold-tolerance related genes in N. spumigena KNUA005. This suggests the possibility of incorporating cold-resistance genes into indigenous cyanobacterial strains for the consistent production of algae-based biofuel during the low-temperature seasons. Therefore, it is needed to determine the cold-tolerance mechanisms in the Arctic cyanobacterium in the next research stage.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.36 no.5
• /
• pp.429-444
• /
• 1991

Cold stress influence plant growth through a wide range of growth characters. Adverse effects of low temperature to plant growth come from results of colligative and complex physiological responses to cold stress. To evaluate more exactly cold tolerance of crop plant, it is needed to observe physiological changes induced by cold stress and to analyze relationships between intraspecific variations in physiological factors related to cold tolerance and the extent of cold tolerance in the field. Therefore, the composition and unsaturation ratio of fatty acids in phospholipid, a constituent of membrane, the transition-temperature in respiratory activity of mitochodria, the chlorophyll fluorescence as a factor related to photosynthesis were investigated in rice plant and data on these factors were compared with the degree of cold tolerance obtained in the field experiment. Also, effects of hardening and Mn++ treatment were evaluated as a method to reduce chilling injuries. The unsaturation ratio of fatty acids, whether rice plants were grown in a natural condition or under the chilling stress, was higher in the cold- tolerant varieties and was significantly correlated with the degree of cold tolerance (1-9) observed in the field experiment. And it was also increased by chilling treatment or hardening treatment, due to a reduction in palmitic acid content and an increase in linolenic acid content. The transition-temperature of respiratory activity of mitochodria isolated from etiolated rice seedlings ($25^{\circ}C$, two week-grown in the dark), was correlated with the degree of cold tolerance in the field, cold -tolerant varieties showing a lower transition-temperature. It was not influenced by growth stages. The intensity of chlorophyll fluorescence was highly correlated with the degree of cold tolerance, cold-tolerant varieties having a higher fluorescence intensity. By foliar application of Mn, the transition-temperature of respiratory activity was lowered as much as 0-2$^{\circ}C$ in all tested varieties. Soil application of Mn induced more significant effect in cold-susceptible varieties with a possibility of reducing chilling injuries. On the whole, there were high correlationships among the degree of cold tolerance, the unsaturation ratio of fatty acids in phospholipid, the transition- temperature of respiratory activity and chlorophyll fluorescence except for a few varieties. The transition- temperature of respiratory activity appeared to be negatively correlated with the unsaturation ratio of fatty acids. and the chlorophyll fluorescence to be positively correlated with the unsaturation ratio. This implies that these physical and physiological factors were very closely related to cold tolerance and can be used as an effective index of the evaluation of cold tolerance of crop plant. But other factors as well as three factors discussed above are needed to be considered colligatively and altogether with a systematic analysis for the more exact evaluation of cold tolerance. in rice cultivars. in rice cultivars.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.20 no.1
• /
• pp.1-6
• /
• 2000

Improvement of cold-tolerance of Italian ryegrass(Lo1ium multiflorum L.) is an important breeding objectivefor enlargement of cultivation area in Korea. To develop the cold-tolerant variety of Italian ryegrass,cold-tolerant clones survived under - 13- - 14$^{\circ}$C of minimum average air temperature in January wereselected in the area of Dun Nae, Kwangwon Province. The 5 lines of Italian ryegrass clones selected werepolycrossed for seed production. Synthetic seeds were examined on growth characters and forage productionsin Suwon from 1996 to 1998, in Unbong 1997 and in Younchun 1998, respectively. The growth charactersand forage productions of Italian ryegrass variety named as "Hwasan 101" were summarized as follow ;ltalian ryegrass variety, Hwasan 101 was tetraploid and showed semi-prostrate growth habit in autumn andwas not only dark green in leaf color and broad in flag leaf width but also excellent in leafiness. Also, itshowed low plant height at first heading date of 20th May and excellent lodging tolerance. Expecially,Hwasan 101 in all trial regions was 80 -90% of winter survivals that was higher than 60 -90% of Florida80 and Marshall varieties. Therefore we estimate that Hwasan 101 can be cultivated in regions that is higherthan -9$^{\circ}$C in minimum average air temperature and lower than 400m in sea level. In forage production ofHwasan 101, fresh weight and total digestible nutrient(TDN) yield were 66,940 and 6,551kghq and drymatter yield was 10,050kgha. In conclusion, Italian ryegrass, Hwasan 101 was medium and late maturingvariety but excellent in cold-tolerance, lodging-tolerance, leafiness, and forage production.(Key words : Italian ryegrass, Cold tolerance, New variety)ian ryegrass, Cold tolerance, New variety)

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.5
• /
• pp.407-412
• /
• 2003

In order to measure an antifreezing tolerance, antifreeze proteins accumulated upon cold acclimation in apoplast were analyzed. As Dongborilho were cold-acclimated for 3 to 74 days there was an abrupt increase in apoplastic proteins up to 30 days and then decrease to the similar levels. Among the known antifreeze proteins, CLP produced in E. coli. and TLP purified from apoplast were used to generate antisera that allow to measure and localize the proteins in leaves of barley. The CLP of 27.7 kDa and TLPs of 6, 26, 27, 30, and 31 kDa were increased in their amounts in apoplast as cold treatment being longer. There was a correlation among the amounts of those proteins accumulated in apoplast and freezing tolerance as shown in field and ion leakage tests for five cultivars. The deposit of CLP was localized in the marginal area and the area adjacent to leaf vescular bundle cells in an increasing manner according to duration of cold acclimation but no variation was observed in terms of it's distribution. Based on the close correlation between levels of antifreeze proteins and degrees of freezing tolerance, the immunological methods was to develop to estimate a freezing tolerance in barley.

• The Korean Journal of Community Living Science
• /
• v.17 no.2
• /
• pp.49-60
• /
• 2006

It was measured the energy expenditure in each season, the cold tolerance in winter and the heat tolerance in summer. Farmers' adaptability to the change of environment was compared with those of city-dwellers such as indoor workers and street cleaners to determine the effect of living environment, especially living temperature, on the health of human body. It turned out that farmers had experienced wide range of temperature that was higher in summer and lower in winter than indoor workers. Farmers and street cleaners showed seasonal adaptation in energy expenditure, which was high in winter and low in summer. However, indoor workers did not show seasonal changes. Energy expenditure had an inverse correlation with the temperature in work place where subjects spend the longer time in a day except in female indoor workers in Seoul. And It was proved that farmers and street cleaners had stronger cold tolerance and heat tolerance than indoor workers.