• The Plant Pathology Journal
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• 제37권1호
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• pp.36-46
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• 2021

Acidovorax citrulli (Ac) is the causal agent of bacterial fruit blotch (BFB) in watermelon, a disease that poses a serious threat to watermelon production. Because of the lack of resistant cultivars against BFB, virulence factors or mechanisms need to be elucidated to control the disease. Glycerol-3-phosphate dehydrogenase is the enzyme involved in glycerol production from glucose during glycolysis. In this study, we report the functions of a putative glycerol-3-phosphate dehydrogenase in Ac (GlpdAc) using comparative proteomic analysis and phenotypic observation. A glpdAc knockout mutant, AcΔglpdAc(EV), lost virulence against watermelon in two pathogenicity tests. The putative 3D structure and amino acid sequence of GlpdAc showed high similarity with glycerol-3-phosphate dehydrogenases from other bacteria. Comparative proteomic analysis revealed that many proteins related to various metabolic pathways, including carbohydrate metabolism, were affected by GlpdAc. Although AcΔglpdAc(EV) could not use glucose as a sole carbon source, it showed growth in the presence of glycerol, indicating that GlpdAc is involved in glycolysis. AcΔglpdAc(EV) also displayed higher cell-to-cell aggregation than the wild-type bacteria, and tolerance to osmotic stress and ciprofloxacin was reduced and enhanced in the mutant, respectively. These results indicate that GlpdAc is involved in glycerol metabolism and other mechanisms, including virulence, demonstrating that the protein has pleiotropic effects. Our study expands the understanding of the functions of proteins associated with virulence in Ac.

• 마이크로전자및패키징학회지
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• 제27권4호
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• pp.47-54
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• 2020

활성 도파로와 수동 도파로의 집적은 광집적 회로의 구성에서 필수적인 요소이다. 이를 구현하기 위한 여러 기술 중 버트 조인트는 상당한 장점을 가지고 있다. 그러나 버트 조인트 접합은 높은 광손실을 야기하며, 두 도파로 간의 정렬에 있어서 정확한 공정 제어가 요구되는 구조이다. 본 논문에서는 레이저 다이오드와 spot size converter (SSC)로 구성된 집적 소자를 시뮬레이션하기 위해 beam propagation method을 이용하였다. 상이한 모드 특성을 갖는 두 SSC를 레이저 도파로와 연결하고, 광결합 효율을 시뮬레이션 하였다. 큰 근접장 모드를 가지는 SSC는 낮은 광결합 효율을 보여주나, 원거리 발산각 패턴이 좁고 더 대칭적이다. 테이퍼 구조의 수동 도파로는 원거리 발산각 패턴을 열화시키지 않고 버트 조인트에서 도파로 오프셋의 무의존성과 광결합 효율을 향상시키기 위해 이용되었다. 이를 바탕으로 89.6%의 높은 광결합 효율과 16°×16°의 좁은 원거리장 발산각을 얻을 수 있었다.

• 한국환경과학회지
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• 제30권3호
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• pp.235-243
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• 2021

This study was conducted to investigate the growth of Dendranthema zawadskii in damaged soils when they are treated with improvement agents. The treatments consisted of a control (unamended field soil) and the application of a loess ball of 1 cm to the field soil. According to the degree of damage the de-icing agent had caused, the soils were divided into 3 areas (based on the yellowing of Pinus densiflora for. multicaulis in soil surveys): H (high saline), M (medium saline), and L (low saline). A total of six treatments were performed: D. zawadskiia plant without soil amendment (H; high saline soil, M; medium saline soil, L; low saline soil), and a D. zawadskiia plant with loess ball on the soil surface (H.L; high saline soil with loess ball, M.L; medium saline soil with loess ball, L.L; low saline soil with loess ball). The results showed that D. zawadskiia growth went from highest to lowest in the order: M.L > L.L > M > L > H.L > H. Plant growth results showed that soils treated with soil amendments (loess ball) were better for D. zawadskii growth than untreated soils.

• Nutrition Research and Practice
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• 제15권2호
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• pp.160-172
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• 2021

BACKGROUND/OBJECTIVES: Nutritional status and food intake during pregnancy and lactation can affect fetal programming. In the current metabolic syndrome epidemic, high-fructose diets have been strongly implicated. This study investigated the effect of maternal high-fructose intake during pregnancy and lactation on the development of metabolic syndrome in adult offspring. SUBJECTS/METHODS: Drinking water with or without 20% fructose was administered to female C57BL/6J mice over the course of their pregnancy and lactation periods. After weaning, pups ate regular chow. Accu-Chek Performa was used to measure glucose levels, and a tail-cuff method was used to examine systolic blood pressure. Animals were sacrificed at 7 months, their livers were excised, and sections were stained with Oil Red O and hematoxylin and eosin (H&E) staining. Kidneys were collected for gene expression analysis using quantitative real-time Polymerase chain reaction. RESULTS: Adult offspring exposed to maternal high-fructose intake during pregnancy and lactation presented with heavier body weights, fattier livers, and broader areas under the curve in glucose tolerance test values than control offspring. Serum levels of alanine aminotransferase, aspartate aminotransferase, glucose, triglycerides, and total cholesterol and systolic blood pressure in the maternal high-fructose group were higher than that in controls. However, there were no significant differences in mRNA expressions of renin-angiotensin-aldosterone system genes and sodium transporter genes. CONCLUSIONS: These results suggest that maternal high-fructose intake during pregnancy and lactation induces metabolic syndrome with hyperglycemia, hypertension, and dyslipidemia in adult offspring.

• 한국정보통신학회논문지
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• 제26권1호
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• pp.146-152
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• 2022

라즈베리 파이나 아두이노를 이용하여 온도, 습도, 기울기, 압력 센서 등에서 수집한 정보는 자동 항온, 항습 시스템 등에 활용되고 있다. 또 농축산업에 활용하여 원격에서 스마트폰만으로 시스템을 제어함으로써 농축산업 종사자들이 편리하게 활용하기도 한다. 일반적으로 온도, 습도는 꺾은선 그래프 등으로 표현하여 변화를 실시간으로 감시하는 역할을 수행하기도 한다. 온도를 시각적으로 표현하는 기술은 최근 코로나19의 발열 검사를 위해 적외선 기기를 이용해 직관적으로 활용되기도 한다. 본 논문에서는 라즈베리 파이와 DHT11 센서에서 수집된 정보를 직관적인 시각화를 통해 공간상의 온도변화를 예측하고 즉각적인 대응이 가능하도록 제안한다. 이를 위해 온도, 습도를 효과적으로 시각화하기 위해 알고리즘을 만들었고, 일부 센서에 결함이 발생하는 경우에도 데이터 표현이 가능하다.

• 대한약침학회지
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• 제25권2호
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• pp.130-137
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• 2022

Objectives: Insulin resistance (IR) is major cause of type 2 diabetes (T2D), and adipokines (e.g., adiponectin, leptin, and resistin) play an important role in insulin sensitivity. Medicinal plants are frequently used for T2D treatment. This study investigates the effect of Artemisia annua L. (AA) extracts on adipokines in mice with high-fat-diet (HFD)/streptozotocin (STZ)-induced T2D. Methods: We divided 60 mice into 12 groups (n = 5 per group): control, untreated T2D, treated T2D, and 9 other groups. T2D was induced in all groups, except controls, by 8 weeks of HFD and STZ injection. The treated T2D group was administered 250 mg/kg of metformin (MTF), while the nine other groups were treated with 100, 200, and 400 mg/kg of hot-water extract (HWE), cold-water extract (CWE), and alcoholic extract (ALE) of AA (daily oral gavage) along with 250 mg/kg of MTF for 4 weeks. The intraperitoneal glucose tolerance test (IPGTT) was performed, and the homeostasis model assessment of adiponectin (HOMA-AD) index and blood glucose and serum insulin, leptin, adiponectin, and resistin levels were measured. Results: Similar to MTF, all three types of AA extracts (HWEs, CWEs, and ALEs) significantly (p < 0.0001) decreased the area under the curve (AUC) of glucose during the IPGTT, the HOMA-AD index, blood glucose levels, and serum insulin, leptin, and resistin levels and increased serum adiponectin levels in the MTF group compared to the T2D group (p < 0.0001). The HWEs affected adipokine release, while the CWEs and ALEs decreased leptin and resistin production. Conclusion: Water and alcoholic AA extracts have an antihyperglycemic and antihyperinsulinemic effect on HFD/STZ diabetic mice. In addition, they decrease IR by reducing leptin and resistin production and increasing adiponectin secretion from adipocytes.

• 대한통합의학회지
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• 제10권3호
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• pp.151-159
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• 2022

Purpose : The purpose of this study was to investigate the structural relationships between fear of missing out, addictive tendencies toward social network services (SNSs), and depression in colleges. Methods : The target subjects were students in colleges across gyeongnam & busan, to whom the purpose of the study was explained and who spontaneously agreed to participate. A survey was conducted with 302 participants over 31 days from March 7, 2022, and data from 299 responses was analyzed. Results : 1. Women felt a higher fear of missing out than men. 2. Women showed greater inability to control their use of SNSs, more SNS-related disorders in daily life, and greater immersion in and tolerance of SNSs when compared to men. 3. Women were more depressed than men. 4. Positive correlations were observed between the fear of missing out and SNS-addictive tendencies, between the fear of missing out and depression, and between SNS-addictive tendencies and depression. Conclusion : A comprehensive review of these findings suggests that women had overall higher levels of isolation fear, SNS-addictive tendencies, and depression than men. Based on this, universities should provide gender-specific educational programs around these issues; this student cohort will ultimately work in healthcare, and this kind of awareness will be essential for treating patients. Considering that the current situation poses unusual challenges due to the COVID-19 pandemic, the study's results can serve as basic data for planning educational programs in the future. Over the coming years, comprehensive and continuous education and counselling relating to the fear of missing out, SNS addiction, and depression will be urgently required.

• 여성건강간호학회지
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• 제28권4호
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• pp.275-285
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• 2022

Purpose: This study explored the experiences of pregnant women with depressed mood participating in a group cognitive behavioral therapy (CBT) program using video communication, based on Beck's cognitive theory. Methods: The participants were six pregnant women out of 13 women who had participated in an 8-session group CBT program using video communication for women with depressed mood (Edinburgh Postnatal Depression score of ≥9). Data were collected from February 20 through March 25, 2021. In-depth individual interviews were conducted through a video conferencing platform at 1 month post-baseline. Thematic analysis was done. Results: Three themes, 10 subthemes, and 38 concepts were derived from experiences of participating in the 4-week group CBT program (twice a week). The first theme, entitled "continuing realization" had subthemes of "a negative and instable self," "a selfish judgment that excludes others," and "a strong belief in self-control." The second theme, entitled "attempt to change for restoration" had subthemes of "shift to rational thinking," "freedom from suppressed beliefs," "tolerance of other people," and "courage for self-expression." The third theme, entitled "departure for a positive life," had subthemes of "emotional healing," "faith in oneself," and "reestablishing the criteria for happiness." Conclusion: Pregnant women with depressed mood expressed that continuing realizations and attempts to change supported their transition toward a positive direction of healing. Thus, they were able to change their distorted thinking into rational thinking through CBT using video communication. These findings support the use of group CBT using video communication with pregnant women who have depressed mood.

• 운동영양학회지
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• 제16권2호
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• pp.101-111
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• 2012

The purpose of this thesis is to investigate whether taurine supplementation in combination with fructose improves both energy metabolism and exercise capacity. Eight collegiate female subjects were recruited for the study. Each subject went through threecross-over designs: control(fluid), fructose, and taurine plus fructose supplementation trials. Subjects received taurine supplementation 100 mg/kg a day for two weeks. After the supplementation, all subjects take 10% fructose at 15 min prior to exercise, immediately before exercise, and every 15 min during exercise. Subjects received 150 ml fluid as placebo during the same procedure. The subjects performed submaximal exercise at the exercise intensity of 60% for 45 min and then 80% of maximal oxygen uptake (VO_2max) until exhaustion time. A 10ml blood sample was taken for measuring the level of glucose, ammonia, lactate, free fatty acids, and insulin every 15 min during exercise at 60% of VO_2max. The blood glucose levels was significantly higher at 45 min and 50 min exercise after supplementation of fructose, and immediately before exercise and 50 min exercise after taurine plus fructose compared to the placebo trial. However, the values tended to be lower in taurine plus fructose supplementation compared to the fructose trial. The levels of both lactate and ammonia were significantly lower compared to the placebo, while the exhaustion time was significantly increased. The level of free-fatty acids was significantly lower at 30, 45, and 50 min after fructoseand fructose plus taurine supplementation compared to the placebo trial. The level of glucagon was significantly lower at 15, 30, 45, and 50 min after fructose and fructose plus taurine supplementation compared to the placebo trial. There was no differences in insulin concentration among three treatments. This thesis concludes that combined taurine and fructose supplementation prior to exercise may improve exercise tolerance time and energy metabolism, lowering the muscle fatigue factors such as lactate and ammonia.

• Plant Biotechnology Reports
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• 제1권1호
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• pp.19-25
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• 2007

To develop an efficient screening method for detection of the transgene in Chinese cabbage (Brassica rapa spp. pekinensis) utilizing Basta spray, optimal conditions for Basta application were examined in this study. Two transgenic Chinese cabbage lines were obtained through Agrobacterium-mediated transformation and used as transgenic positive controls in the Basta screening experiment. Differential concentrations of glufosinate-ammonium were sprayed into three different growth stages of 12 commercial Chinese cabbage cultivars. The results showed that no plants could survive higher than 0.05% glufosinate-ammonium, and plants at the 2-3 leaf stage were most vulnerable to glufosinate-ammonium. On the other hand, no damage was observed in the transgenic control plants. Reliability of the Basta spray method was proven by showing perfect co-segregation of the tolerance to glufosinate-ammonium and the presence of the bar gene in T₁ segregating populations of the transgenic lines, as revealed by both PCR and Southern blot analyses. Using the developed Basta screening method, we tried to investigate the transgene flow through pollen dispersal, but failed to detect any transgene-containing non-transgenic Chinese cabbages whose parents had been planted adjacent to transgenic Chinese cabbages in field conditions. However, the transgene was successfully detected using Basta spray from the non-transgenic plants bearing the transgene introduced by hand-pollination. Since the Basta spray method developed in this study is easy to apply and economical, it will be a valuable tool for understanding the mechanism of gene flow through pollen transfer and for establishing a biosafety test protocol for genetically modified (GM) Chinese cabbage cultivars.