• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2004.08a
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• pp.208-216
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• 2004

Since 2000, the authors have been developing remote radar network system to observe the vessel traffic in Tokyo Bay. In December 2002, the first operational remote radar station was set at the National Defense Academy in Yokosuka, and vessel traffic observation was started. However, it was impossible to perform accurate observation in the northern part of Tokyo Bay by this Yokosuka radar station only. In September 2003, the second remote radar station and AIS receiving station were installed at Higashi Ogishima in Kawasaki. This second radar enabled us to carry out accurate observation in that area. Both radars can be remotely controlled from the monitoring station in Tokyo University of Marine Science and Technology. On September 30 and October 1,2003, the vessel traffic observation was carried out using both radars. Combining radar images observed by both radars, the ships' tracks were taken and the dangerous ships were extracted by using SJ value and Bumper Model. The time changes of dangerous ship density in some areas in Tokyo Bay and utilization ratio of the traffic routes were also investigated. In addition, analyzing the AIS date received at Kawasaki station, the positions and speed vectors of the ships equipped with AIS were shown.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.416-417
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• 1989

• Journal of Animal Science and Technology
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• v.59 no.5
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• pp.9.1-9.1
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• 2017

• Journal of Radiation Protection and Research
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• v.49 no.2
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• pp.65-67
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• 2024

This review is based on articles published in Japanese Journal of Health Physics on "Radiation-exposed medical care and waste" related to Tokyo Electric Power Company Incorporated (TEPCO)'s Fukushima Daiichi Nuclear Power Station accident. Here, we have considered three original articles; one technical data, one special article, one 50^th anniversary article, one preface, three topics, and two cases of From Japan to the World (J to W). These articles have reported the system and standards that were established after the accident. Moreover, they have summarized rare experiences such as the fumbling response at the time and the evaluation of samples in that disaster. These articles constitute valuable records of the situation.

• Journal of Embryo Transfer
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• v.26 no.4
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• pp.237-244
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• 2011

Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vitro models. RT-PCR and real time PCR analysis respectively revealed that LH had no effect on c-Ski mRNA expression in the cultured granulosa cells regardless of LH treatment. Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-Ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally. Moreover, expression of mRNA of Arkadia, an E3 ubiquitin ligases, in luteinizing granulosa cells in vivo was assessed by realtime-PCR. The levels of Arkadia mRNA expression were unchanged during follicular growth and postovulatory luteinization. These findings suggest that Ski protein level may be regulated during luteinization at translational and/or post-translational level but not by Arkadia.

• Journal of Embryo Transfer
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• v.26 no.4
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• pp.229-235
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• 2011

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski protein is implicated in proliferation/differentiation in a variety of cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of this study was, by means of immunohistochemical methods, to locate Ski protein in the rat ovaries during ovulation and corpora lutea (CL) formation to predict the possible involvement of Ski in luteinization. In addition, we performed to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vivo models. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). These results indicate that Ski is profoundly expressed in the luteinized granulosa cells and luteal cells of CL during luteinization, and suggest that Ski may play a role in luteinization of granulosa cells.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.355-361
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• 2011

Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinization to predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadotropin to immature female rat, and luteinization was induced by human chorionic gonadotropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.341-348
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• 2011

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. The aim of the present study was to locate Ski protein in rat ovaries in order to predict the possible involvement of Ski in follicular development and atresia. First, expression of c-Ski mRNA in the ovaries of adult female rats was confirmed by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and proliferating cell nuclear antigen (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not in PCNA-positive granulosa cells, was also verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin (eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles, and suggest that Ski plays a role in apoptosis of granulosa cells during follicular atresia.

• Proceedings of the Korean Society of Crop Science Conference
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• 1995.05a
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• pp.68-69
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• 1995

• Nuclear Engineering and Technology
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• v.49 no.2
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• pp.442-449
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• 2017

Risk management of the Fukushima Daiichi Nuclear Power Station decommissioning is a great challenge. In the present study, a risk management framework has been developed for the decommissioning work. It is applied to fuel assembly retrieval from Unit 3 spent fuel pool. Whole retrieval work is divided into three phases: preparation, retrieval, and transportation and storage. First of all, the end point has been established and the success path has been developed. Then, possible threats, which are internal/external and technical/societal/management, are identified and selected. "What can go wrong?" is a question about the failure scenario. The likelihoods and consequences for each scenario are roughly estimated. The whole decommissioning project will continue for several decades, i.e., long-term perspective is important. What should be emphasized is that we do not always have enough knowledge and experience of this kind. It is expected that the decommissioning can make steady and good progress in support of the proposed risk management framework. Thus, risk assessment and management are required, and the process needs to be updated in accordance with the most recent information and knowledge on the decommissioning works.