• Asian-Australasian Journal of Animal Sciences
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• 제26권7호
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• pp.930-934
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• 2013

The growth performance of embryo-transferred Japanese Black calves that were born from, and suckled by, Japanese Shorthorn cows in a cow-calf grazing system (BS-group, n = 5) was compared to that of Japanese Black calves from Japanese Black cows in a cowshed (BB-group, n = 5). The daily weight gain from birth to 1 month was higher in the BS-group than in the BB-group (p<0.01), and the same trend (p<0.05) was observed at 2 and 3 months of age. This resulted in body weight that was significantly higher for the BS-group between 1 and 3 months of age than what was observed for the BB-group (p<0.05). Heart girth was significantly greater in the BS-group than in the BB-group throughout the experimental period (p<0.01), and chest depth and withers height in the BS-group were significantly greater from 2 to 4 months of age (p<0.05) and at 4 months of age only (p<0.05). No difference in body length (p>0.05) was observed between the groups. These results suggest that the maternal effect of Japanese Shorthorn cows was positive for embryo-transferred Japanese Black calf growth during the early suckling stage. As Japanese Black calves are traded at a high price on the Japanese market, we conclude that this proposed production system is likely to improve the profitability of herd management in upland Japan.

• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1303-1308
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• 2004

The beef color stability during display of two muscles, m. longissimus thoracis and m. semitendinosus, of Japanese Shorthorn steers (n=14) was compared with that of Japanese Black steers (n=14). The beef color of each carcass was evaluated according to the Japanese Grading Standards at 24 h post mortem. Steak samples from muscles were over-wrapped with PVC film and displayed under fluorescent lights at $4^{\circ}C$ for 9 days. Metmyoglobin percentages of steak samples were determined at days 0, 3, 6 and 9. The overall grade of beef color of the carcasses of Japanese Shorthorn steers was significantly (p<0.05) lower than that of Japanese Black steers. The metmyoglobin percentages during the display of two muscles of Japanese Shorthorn steers were significantly (p<0.05) lower than those of Japanese Black steers. These results suggested that though beef color evaluation of the carcasses of Japanese Shorthorn steers was lower than that of Japanese Black steers, the beef color stability during the display of the muscle of Japanese Shorthorn steers was higher than that of Japanese Black steers.

• 한국언어정보학회:학술대회논문집
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• 한국언어정보학회 2007년도 정기학술대회
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• pp.471-480
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• 2007

Japanese does not exhibit deontic-epistemic polysemy which is recognized among typologically different languages. Hence, in Japanese linguistics, it has been debated which of the two types of modality is more prototypical. This study brings Chinese learner's acquisition data of Japanese modality to bear on the question of which of the two types of modality is more prototypical, using the Competition Model (Bates and MacWhinney 1981). The Competition Model notion of 'cues' as processing strategy adopted by learners reveals the continuity/discontinuity between these two modality domains.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1055-1062
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• 2012

Two SNPs, i.e. L127V and T172M, of bovine growth hormone (GH) causing the presence of GH gene haplotypes A, B, and C was previously shown to alter intramuscular fatty acid (FA) composition in Japanese Black (JB) heifers. To determine the SNP effect on somatotropic hormone concentration and lipogenesis, we measured plasma GH, insulin, and insulin-like growth factor-1 (IGF-1) concentrations. We also measured mRNA levels of fatty acid synthase (FASN), stearoyl-coA desaturase (SCD), and sterol regulatory element binding proteins-1 (SREBP-1) and FA composition in diaphragm tissues. Heifers with genotype CC had the lowest plasma insulin concentration and FASN and SCD mRNA levels among genotypes. FASN mRNA levels in haplotype A tended to positively correlate with saturated FA (SFA) content and negatively correlated with C18:2 and unsaturated FA (USFA) contents. SCD mRNA levels in haplotype A positively correlated with monounsaturated FA (MUFA) contents and negatively correlated with C18:0 content. They also tended to positively correlate with C16:1, C18:1, and USFA contents and USFA/SFA ratio and negatively correlate with SFA content. Taken together, GH gene polymorphism affects the lipogenic genes expression levels and their relationships with fatty acid compositions in diaphragm tissues of JB heifers at 31 months of age.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.420-426
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• 2005

Effect of pasture finishing on $\alpha$-tocopherol and $\beta$-carotene concentrations, drip loss, cooking loss, Warner-Bratzler shear force, fatty acid composition, meat color and metmyoglobin percentage of m. longissimus thoracis of Japanese Shorthorn steers was studied. Japanese Shorthorn steers (n=8), fattened indoors with a concentrate-based diet until they were 19 months of age were divided into two groups of four steers. Steers of the concentrate-fed group were fattened indoors with a concentrate-based diet until they were slaughtered. Steers of the pasture-fed group were fattened on pasture until they were slaughtered. The $\alpha$-tocopherol and $\beta$-carotene concentrations in the muscle of the pasture-fed group were higher (p<0.05) than those of the concentrate-fed group. The drip loss of the muscle of the pasture-fed group was lower (p<0.05) than that of the concentrate-fed group. Compared with the concentrate-fed group, the concentration of peroxidisable lipids (fatty acids with three or more unsaturated bonds) in the muscle of the pasture-fed group was high (p<0.05). The metmyoglobin percentage during display of the muscle of the pasture-fed group was higher (p<0.05) than that of the concentrate-fed group. These results suggested that pasture finishing decreased drip loss of the beef but lowered meat color stability.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1430-1434
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• 2005

We conducted two experiments to assess the effect of transfer from housing to grazing on stress hormone secretion in cattle using urine samples. In a preliminary experiment, urine samples were collected following an adrenocorticotrophic hormone (ACTH) challenge, and cortisol levels in urine were compared with the levels in plasma. In a second experiment, urinary cortisol was measured before and after the start of a grazing season in 6 Japanese Shorthorn cows, all of which had experienced grazing before. In experiment 1, urinary cortisol showed a pattern of changes similar to that of plasma with a 0.5-h temporal lag time, and the peak levels were 4 to 10 times higher than the basal levels. In experiment 2, the urinary cortisol levels in cows did not change after the cows were let out to pasture, with no decreases in body weight. This study suggests that the transfer from housing to grazing did not affect physiological responses to cause high excretion of urinary cortisol in grazing-experienced cattle using a non-invasive sampling method.

• Asian-Australasian Journal of Animal Sciences
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• 제28권8호
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• pp.1084-1089
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• 2015

Chemerin, highly expressed in adipose and liver tissues, regulates glucose and lipid metabolism and immunity in these tissues in ruminants and mice. Our previous reports showed that chemerin is involved in adipogenesis and lipid metabolism in adipose tissue as an adipokine. The aim of the present study was to identify single nucleotide polymorphisms (SNPs) in the coding region of the chemerin gene and to analyze their effects on carcass traits and intramuscular fatty acid compositions in Japanese Black cattle. The SNPs in the bovine chemerin gene were detected in 232 Japanese Black steers (n = 161) and heifers (n = 71) using DNA sequencing. The results revealed five novel silent mutations: NM_001046020: c.12A>G (4aa), c.165GT (92aa), c.321 A>G (107aa), and c.396C>T (132aa). There was no association between 4 of the SNPs (c.12A>G [4aa], c.165GG [107aa], and c.396C>T) and carcass traits or intramuscular fatty acid compositions. Regarding the remaining SNP, c.276C>T, we found that cattle with genotype CC had a higher beef marbling score than that of cattle with genotype CT, whereas cattle with genotype CT had a higher body condition score (p<0.10). Further, cattle with genotype CC had significantly higher C18:0 content in their intramuscular fat tissue than that of cattle with genotype CT (p<0.05). On the other hand, cattle with genotype CT had significantly higher C14:0 and C16:0 content in their intramuscular fat tissue (p<0.05). Moreover, the number of individuals carrying the minor allele of c.276C>T SNP is small. It is suggested that the c.276C>T SNP of the chemerin gene has potential in cattle breeding using modern methods, such as marker assisted selection. So, further functional and physiological research elucidating the impact of the chemerin gene on bovine lipid metabolism including fatty acid synthesis will help in understanding these results.

• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1828-1832
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• 2018

Objective: Environmental change is one of the stressful events in livestock production. Change in environment disturbs cow behavior and cows require several days to regain a stable behavioral pattern. Sleeping posture (SP) and lying posture (LP) have been used as indicators for animal that are relaxed and well-acclimated to their environment. The aim of this study was to examine the time required by Japanese black cows for stabilization of SP and LP after moving into new environment. Methods: Seven pregnant Japanese black cows were used. Cows were moved into new tie-stall shed and their sleeping and lying posture measured 17 times during 35 experimental days. Both SP and LP were detected by accelerometer fixed on middle occipital and hip-cross, respectively. Daily total time, frequency, and average bout of both SP and LP were calculated. Results: Daily SP time was the shortest on day 1 and increased to the highest on day 3. It then decreased until day 9, after that stabilized about 65 min/d till the end of experiment. Daily LP time changed in same manner as daily SP time. The average SP bout was the longest on day 1, and then decreased to stable level on day 7. On the other hand, the average LP bout was the shortest on day 1, and it increased to stable level on day 7. Conclusion: These results showed that pregnant Japanese black cows needed 1 week to stabilize their SP. However, there were different change patterns between the average SP and LP bout, even though the change pattern of daily SP and LP time were similar.

• 말소리와 음성과학
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• 제15권1호
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• pp.35-45
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• 2023

일본어의 전통적인 어두 폐쇄음은 파열 전에 성대 진동을 동반하는 유성음과 파열 후에 약간의 기음을 동반하는 무성음으로 이분된다. 한편 도호쿠지방의 유성음은 어느 세대나 파열 전에 성대 진동을 동반하지 않고 무성화한 유성음으로 실현되어 다른 지역과 대조를 이룬다. 무성화한 유성음은 voice onset time(VOT)이 양값으로 나타나고 그러면 기존의 무성음의 VOT와 충돌하게 되어 카테고리 구별에 영향을 미치게 된다. 이에 대해 도호쿠지방의 화자는 생성 시에 다른 지역과는 달리 폐쇄음 구별에 후속 모음의 fundamental frequency(F0)를 적극적으로 사용하는 것이 여러 연구에 의해 확인되었다. 본 연구는 인지면에서도 F0가 폐쇄음 구별에 중요한 역할을 하고 있는지를 밝히기 위해 VOT와 함께 검토한 것이다. VOT와 F0를 재합성한 자극음을 사용하여 도호쿠지방 청자를 대상으로 조건을 달리한 여러 개의 지각실험을 실시하였다. 결과에서는 무의미어의 경우 지역차(도호쿠 지방 vs.주부 지방)는 유의하지 않았으나 유의미어에서는 어휘에 따라 F0 사용에 유의한 차이가 있었으며 이러한 차이는 F0를 적극적으로 사용하는 몇몇의 청자들에게서 기인하는 것으로 밝혀졌다. 논의에서는 이들이 혁신 청자들로 여겨지며 이들을 중심으로 폐쇄음 지각에 F0 역할이 일반화되고 지각특성으로서 F0가 확립될 가능성에 대해 추론해 보았다.

• Animal Bioscience
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• 제36권11호
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• pp.1769-1774
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• 2023

Objective: Resting comfort may influence sleep-related lying postures in cattle. This study aimed to investigate the effects of bed cleanliness on tucked (TSP) and extended (ESP) head positions in sleep-related lying postures. Methods: The study was conducted over two experimental periods. In each period, four Japanese Black fattening cattle were assigned to the cleaning treatment (CL), in which bedding material was replaced once during each experimental period. Four cattle were assigned to the control treatment (CON) with no bed cleaning. Daily duration, bout frequency, and bout length of sleep-related lying postures were measured, and bed moisture, ammonia concentration in the air, plasma cortisol, and serotonin concentration were also measured within one week before and after cleaning treatment in each period. Results: The bed moisture and ammonia concentrations decreased in CL after bed cleaning. Following bed cleaning, the duration and bout frequency of TSP in CL decreased compared to that observed in CON, whereas ESP in CL increased after bed cleaning. Total duration of sleep-related lying postures and cortisol and serotonin levels did not differ between CL and CON. Conclusion: These results suggest that cattle in sleep-related lying postures shifted from tucked head positions to extended head positions in response to improved bed cleanliness.