• 한국연초학회지
• /
• 제27권1호
• /
• pp.100-106
• /
• 2005

To obtain the optimum condition for analysis of 10 alkaloids in tobacco leaves, such as nicotine, nornicotine, anatabine, anabasine, myosmine, cotinine, 2,3'-dipyridyl, $\beta-nicotyrine,\;\beta-nornicotyrine\;and\;\beta-formylnornicotin$, 5 types of extraction method were investigated by GC-FID and GC/MS. The optimum condition of alkaloid extraction was achieved by using methanol:dichloromethane(1:3, v/v) after NaOH treatment. The use of mass selective detector (MSD) provided unambiguous nicotine related alkaloid analysis. Alkaloids in various tobacco leaves were extracted with the optimum extraction condition and quantified by GC/MS/SIM mode. Compared with concentrations of alkaloids among the various tobacco leaves, the concentration of alkaloids was generally in the order burley > flue-cured > oriental tobacco. In flue-cured tobacco leaves, the order of concentration of alkaloids was nicotine > anatabine > nornicotine > $\beta-nicotyrine\;>\;\beta-formylnornicotine\; >\;myosmine\;>\;2,3'-dipyridyl\;>\;cotinine\;>\;anabasine\;>\;\beta-nornicotyrine$. However, in the case of burley and oriental tobacco leaves, the concentration of nornicotine was higher than that of anatabine.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XIII)
• /
• pp.186-189
• /
• 2003

Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a potent immunogen as well as major virulence factor. In order to express the recombinant urease in tobacco plants, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a plant expression vector. The recombinant vector was transformed to tobacco plants. The integration of the recombinant plasmids into tobacco chromosomal genome was verified by genomic PCR. Expression to mRNA was confirmed by Northern blot analysis, and expression to recombinant urease protein was observed by Western blot analysis. These results showed that the recombinant urease can be produced in tobacco plants and will be tested for immune response to use as an edible vaccine.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권12호
• /
• pp.7065-7067
• /
• 2013

Background: Tobacco use is one of the leading causes of preventable death across the world today and the smoking rates among men in the Arab world are amongst the highest in the world. Smoking of dokha, a traditional Arab tobacco is common in some of the Middle East countries such as the United Arab Emirates and Iran. This review focuses on the prevalence, pattern and health effects of dokha use in the Middle East. For this purpose an electronic search was performed in the following databases and websites: MEDLINE, EMBASE, Proquest and Google scholar up to December 2012. The search strategy was based on Internet search for the synonyms of dohka or midwakh. From the results of the review it emerged that younger people are the most common users of dokha. Hence effective outreach health education programs, targeting adolescents, especially school students before they take up the habit of smoking, may thus curb the emergence of the problem.

• 한국학교보건학회지
• /
• 제28권2호
• /
• pp.99-110
• /
• 2015

Purpose: This study aims to investigate the association between suicidal thoughts and the co-occurrence of alcohol, tobacco, and drug use among adolescents. Methods: This study used the data of the Korea Youth Risk Behavior Web-based Survey from 2008 through 2014. The subjects of this study were 517,866 students, in total (Male 266,500, Female 251,366). Frequency analysis was conducted on the data about the subjects' general characteristics and their experience of smoking, drinking, and drug use. The relationship between suicidal thoughts and the co-occurrence of alcohol, tobacco, and drug use was analyzed through ${\chi}^2-test$ and logistic regression. The analyses were done using SAS 9.4. Results: According to the study, adolescents who used alcohol, cigarettes, and drugs at the same time engaged in suicidal ideation 3.69 times more than their peers who didn't use such substances (95% CI: 3.40~4.00, p<.001). Also, adolescents who used only tobacco, only alcohol, and only drugs engaged in suicidal ideation 1.49 times (95% CI: 1.43~1.56, p<.001), 1.28 times (95% CI: 1.26~1.31, p<.001) and 2.34 times (95% CI: 2.04~2.69, p<.001) more than the students who didn't use any substances, respectively. Conclusion: Adolescents' suicidal ideation and the co-occurrence of alcohol, tobacco, and drug use had a statistically significant relationship.

• Membrane and Water Treatment
• /
• 제5권1호
• /
• pp.31-40
• /
• 2014

Papermaking tobacco sheet is an important reclaimed process for cigarette making. Traditionally, the pressure driven membrane was often used to isolate the effective compounds from the tobacco sheet extract. However, this method is difficult to remove small ionic compounds. Besides, membrane fouling is a major problem for effective use of these pressure driven membrane technologies. In this study, the electrodialysis process is used to removal the chloride ions and nitrate ions, thus the smoking quality of papermaking tobacco sheet extract can get improved. Three types of electrolytes ($Na_2SO_4$, NaCl and HCl) are chosen to prevent the generation of precipitation. The results indicate that 0.1mol/L HCl at current density of $30mA/cm^2$ is the optimal condition for the electrodialysis process. The removal rates of the Cland $NO{_3}^-$ in tobacco sheet extract are 97% and 98.4%, respectively. The electrodialysis process cost was estimated to be 0.11$/L. Naturally, electrodialysis is not only technological feasible, environmental-friendly and economical-attractive for tobacco extract treatment.

• 성인간호학회지
• /
• 제17권4호
• /
• pp.529-538
• /
• 2005

Purpose: This study was conducted to 1) find out the frequency of tobacco control intervention, barriers, and facilitators. 2) compare the differences in tobacco control intervention, barriers, and facilitators between oncology nurses and general nurses. Method: A sample was composed of 96 oncology nurses and 284 general nurses. The survey questionnaire was mailed out to nurses who were working at the randomly selected hospitals throughout the country. The questionnaire was adopted from the study of national survey on oncology nurse's tobacco interventions in United States by Sarnar, et al.(2000). Results: Oncology nurses were found to provide tobacco control interventions more frequently comparing to the general nurses. "Patient not motivated to quit smoking", "Lack of time", "Lack of recognition/rewards", were the most commonly identified barriers. "Patient wants to quit", "Adequate time", "Confidence in ability help people to stop smoking", were the most commonly identified facilitators. Conclusions: Although oncology nurses are in an important position in delivering tobacco interventions and providing resources, their participation in consistent delivery of a tobacco control intervention was less than desirable. To help nurses participate in the assessment of tobacco use and interventions for cessation, the development of educational program is necessary.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권4호
• /
• pp.1659-1665
• /
• 2014

The present study conducted with 100 oral cancer patients at a private tertiary care hospital in Delhidemonstrated that stage III cancer was associated with longer use of tobacco and poor oral hygiene. There was also statistically significant association (p<.05) between consumption of tobacco and alcohol. More than 60% treatment expenditure was on surgery followed by accommodation (9%) and investigations (8%). The effect of tobacco was well known among patients as 76% of the patients knew that common cancer in tobacco chewer is 'oral cancer', 22% of the patients however responded that they did not know which cancer is common in tobacco chewers. 58% said that they learnt about ill effects of tobacco from media while 24% said they learnt from family and friends. Out of 78 tobacco users, 60 (77%) said that they never received help to quit tobacco while 18(23%) have received help to quit.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권sup1호
• /
• pp.37-42
• /
• 2016

Studies have shown that smoking is a learnt behavior, often initiated during adolescence. This paper aims to describe tobacco-related knowledge, attitude and associations among school adolescents aged 13-15 with exposure to anti-smoking information. Using data from the Global Youth Tobacco Survey (GYTS) in Viet Nam, 2014, knowledge was measured through 4 questions about tobacco use, and attitude was assessed through 3 questions on personal, social and environmental aspects. Students giving most anti-tobacco responses to all questions were considered as having correct knowledge or appropriate attitude or both. Access to anti-smoking information was determined by exposure to any media messages on tobacco control during the past 30 days and teaching in school about the danger of tobacco use during the past 12 months. A substantial percentage of students thought that being near others who smoke might be harmful to them and smoking is harmful to health (89.4% and 89.6% respectively). However, only 46.4% reported that it is definitely difficult to quit smoking and 66.9% thought that smoking for only 1 or 2 years, once stopped, is harmful to health. Slightly more than half of the respondents reported appropriate attitude that young smokers have fewer friends than others and smoking makes them less attractive and less comfortable at social events. Noticing anti-smoking messages in the media together with having lessons in school about the dangers of tobacco substantially increased the likelihood of having correct knowledge, appropriate attitude and both. Despite relatively high awareness about smoking harms, effective educational communication is still highly needed to improve the level of comprehensive knowledge and an appropriate attitude regarding tobacco use.

• 한국연초학회지
• /
• 제23권1호
• /
• pp.90-93
• /
• 2001

In order to save hanging labor hours and reduce work intensity for bulk curing of the flue-cured tobacco, and electric hoist was developed. It consists of a pair of square steel frames with side plates and rolling casters to move back and forth on the second tire of bulk barn, and wire rope type lift was attached to the beneath of its top center. The lift driven by DC motor could be controlled by operating switch with one limit switch and control box, and a scissors shaped gripper was deviced to grip tobacco rack to the end of wire rope. As the results of experiments with electric hoist, labor hours for hanging could be saved by 30 to 40% as compared with those in conventional method. With simple and light structure, it was possible for even woman to load the harvested tobacco, and it could be recommended for farmers to use this device with reasonable price.

• 한국연초학회지
• /
• 제20권1호
• /
• pp.124-130
• /
• 1998

This study was conducted to evaluate the ability of anion generating air cleaner to remove gases, vapor and particles from closed room contaminated with environmental tobacco smoke (ETS). The measurements covered particle sizes of 13.8-542.5nm, particle concentration, surface area, volumes UVPM, FPM, solanesol, and the following gases and vapor; carbon dioxide, carbon monoxide, nicotine, and 3-ethenylpyridine. Tobacco smoke was generated and mixed in a closed room in which the airflow rates were in the range of 0.00-0.04 m/s. The anion generating air cleaner was startedl and the decay rates for the gases, vapor and particles were measured, When the use of anion generating air cleaner, solid components of ETS, such as respirable suspended particle (RSP), utraviolet particulate matter (UVPM, fluorescent particulate matter (FPM) and solanesol was sharply decreased, and vapor phase components of ETS, such as nicotines 3-ethenylpyidine were modelately decreased by time elapse. Even the use of anion generation air cleaner, the decreasing rate of carbon dioxide concentration was similar with control, and the decreasing rate of carbon monoxide was slower than that of control. Our results indicated that the use of anion generting air cleaner had an effect on reduction of solid and vapor components from ETs, but it had no effect on gaseous components of ETS.