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Antimicrobial Activity of the Sargassum fulvellum Ethanol Extract and the Effect of Temperature and pH on Their Activity (모자반 에탄올 추출물의 항균활성과 열 및 pH 안정성)

  • Yoon, So-Young;Lee, So-Young;Kim, Koth-Bong-Woo-Ri;Song, Eu-Jin;Lee, So-Jeong;Lee, Chung-Jo;Park, Na-Bi;Jung, Ji-Yeon;Kwak, Ji-Hee;Nam, Ki-Wan;Ahn, Dong-Hyun
    • Korean Journal of Food Science and Technology
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    • 제42권2호
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    • pp.155-159
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    • 2010
  • The antimicrobial activity of Sargassum fulvellum (SF) was investigated using the agar diffusion assay and MIC test. In addition, the stability of this activity under extreme heat and pH conditions was examined. The SF ethanol extract was shown to display strong antimicrobial activities against B. subtilis, C. perfringens, L. plantarum, S. aureus, L. monocytogenes, S. cerevisae and C. tropicalis in the agar diffusion assay at the concentration of 4 mg/mL. The MIC value of the SF ethanol extract against the tested microbes ranged from 0.05 to 0.0063%. In the heat and pH stability test, the antimicrobial activity of the SF ethanol extract was not altered when the temperature was maintained at $121^{\circ}C$ for 15 min, and it was also not affected in the pH range of 2-10. These results suggest that the SF ethanol extract is highly stable against drastic changes in temperature and pH.

Evaluation of N2O Emissions with Different Growing Periods (Spring and Autumn Seasons), Tillage and No Tillage Conditions in a Chinese Cabbage Field (배추의 재배시기와 경운 유.무에 따른 아산화질소 배출 평가)

  • Kim, Gun-Yeob;Jeong, Hyun-Cheol;Shim, Kyo-Moon;Lee, Seul-Bi;Lee, Deog-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • 제44권6호
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    • pp.1239-1244
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    • 2011
  • Importance of climate change and its impact on agriculture and environment has increased with a rise of greenhouse gases (GHGs) concentration in Earth's atmosphere. Nitrous oxide ($N_2O$) emission in upland fields were assessed in terms of emissions and their control at the experimental plots of National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA) located in Suwon city. It was evaluated $N_2O$ emissions with different growing periods (spring and autumn seasons), tillage and no tillage conditions in a chinese cabbage field. The results were as follows: 1) An amount of $N_2O$ emissions were high in the order of Swine manure compost>NPK>Hairy vetch+N fertilizer. By tillage and no tillage conditions, $N_2O$ emissions were reduced to 33.7~51.8% (spring season) and 31.4~76.7% (autumn season) in no-tillage than tillage conditions. 2) In autumn season than those spring season, $N_2O$ emissions at NPK, hairy vetch+N fertilizer and swine manure compost were reduced to 49.6%, 39.0% and 60.0%, respectively, in tillage treatment and 59.5%, 70.6% and 58.7%, respectively, in no-tillage treatment. 3) $N_2O$ emission measured in this study was 15.2~86.4% lower with tillage and no tillage treatments than that of the IPCC default value (0.0125 kg $N_2O$-N/kg N).

Estimation of Genetic Parameter for Milk Production and Linear Type Traits in Holstein Dairy Cattle in Korea (국내 Holstein 젖소의 유생산 형질과 유방 및 지제 선형심사 형질에 대한 유전모수 추정)

  • Won, J.I.;Dang, C.K.;Lim, H.J.;Jung, Y.S.;Im, S.K.;Yoon, H.B.
    • Journal of agriculture & life science
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    • 제50권1호
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    • pp.167-178
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    • 2016
  • This study was conducted to estimate genetic parameters for milk production and linear type traits in Holstein dairy cattle in Korea. The data including milk yields, fat yields, protein yields, fat percent, protein percent, somatic score and 15 linear type traits for 10,218 first parity cows collected by Dairy Cattle Improvement Center, National Agricultural Cooperative, Korea, which were calving from January 2009 to April 2013. Genetic and error (co)variances between two traits selected form 19 traits were estimated using bi-trait pairwise analyses with WOMBAT package. The estimated heritabilities for milk yield(MY), fat yield(FY), protein yield(PY), fat percent(FP), protein percent(PP), somatic cell score(SCS), udder depth(UD), udder texture(UT), median suspensory(MS), fore udder attachment(FUA), front teat placement (FTP), rear attachment height(RAH), rear attachment width(RAW), rear teat placement(RTP), front teat length(FTL), foot angle(FA), heel depth(HD), bone quality(BQ), rear legs side view(RLSV), rear legs rear view(RLRV) and locomotion(LC) were 0.128, 0.144, 0.100, 0.273, 0.333, 0.090, 0.179, 0.066, 0.104, 0.109, 0.127, 0.099, 0.059, 0.069, 0.154, 0.014, 0.010, 0.052, 0.065, 0.175 and 0.031, respectively. Among the genetic correlations, UD, UT, FTP, RAW, FTL, FA and RLSV with MY were -0.334, 0.271, 0.445, 0.544, 0.076, -0.281 and -0.228, respectively, and MS, FTP, RTP, FTL, FA, BQ, RLSV, RLRV and LC with PP were -0.147, -0.182, -0.262, -0.136, 0.355, 0.311, 0.135, 0.233 and 0.143, respectively. Especially, MY had the highest positive genetic correlation with RAW (0.544), while SCS had the highest negative genetic correlation with LC (-0.603). FP had negative genetic correlation with most udder traits, whereas, FP had positive genetic correlation with leg and hoof traits (0.056 - 0.355).

Benign Adenomyoepithelioma of the Breast: Imaging Characteristics (유방의 양성 선근상피세포종: 영상 특징)

  • So Ra Shin;Eun Young Ko;Boo-Kyung Han;Eun Sook Ko;Ji Soo Choi;Haejung Kim
    • Journal of the Korean Society of Radiology
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    • 제84권2호
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    • pp.398-408
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    • 2023
  • Purpose This study aimed to evaluate the radiological and clinical characteristics of benign adenomyoepitheliomas of the breast. Materials and Methods Over the last 20 years, 120 patients were histologically diagnosed with breast adenomyoepithelioma (AME) at our institution. We excluded 43 patients who were incidentally diagnosed during mastectomy for breast cancer, 28 who underwent percutaneous biopsy without further excision, and 8 who had biopsy-confirmed benign AME and were found to have another pathology after complete excision. We retrospectively reviewed the clinical records and radiological findings of the remaining 41 patients with histologically diagnosed benign breast AMEs after complete excision. Results All 41 patients underwent US; 38 underwent mammography (MG) and US; and 18 underwent MG, US, and MRI. MG detected 38 cases with a round or oval shape (56%), and mass (89%), were non-circumscribed (62%), hyperdense (53%), and without microcalcifications (95%). Breast US revealed suspicious masses (98%) with a non-circumscribed margin (66%), hypoechogenicity (43%), and intratumoral vascularity (63%). All lesions on breast MRI showed suspicious masses (100%) with ill-defined margins (61%), and 84% showed wash-out kinetics. Benign AMEs showed suspicious features of Breast Imaging Reporting and Data System (BI-RADS) category 4 or 5 in 83%-95% of the MG, US, and MRI. Sixteen of the 41 cases were misdiagnosed on the initial core needle biopsy and two were diagnosed as malignancy. Conclusion Benign breast AME often shows suspicious radiological features mimicking a malignant mass on MG, US, and MRI. Differentiating benign AME from other pathologies might be difficult on core needle biopsy, and complete excision is needed for a correct diagnosis.

Monitoring of Pesticide Residues and Heavy Metals in Fermented Liquor in Ulsan (울산지역 유통 발효주의 잔류농약 및 중금속 실태조사)

  • Hee-Jung Kim;Kyoung-Jin Kim;Min-Kyung Kim;Geum-Bi Kim;Su-Hee Kim;Young-Kyung Jo;Ju-Eun Park;So-Yeon Jeong;Won-Dug Seo;Young-Sun Choi
    • Journal of Food Hygiene and Safety
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    • 제39권3호
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    • pp.250-259
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    • 2024
  • In this study, we investigated in pesticide residues and heavy metals in fermented liquor products (wine, beer, makgeolli). Targeted analysis of 400 pesticide residues in the sample was performed using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method, followed by gas chromatography-tandem mass spectrometry (MS/MS) and LC-MS/MS. The contents of heavy metals (Pb, Cd) were determined by ICP-MS using the microwave method. The mercury was measured using a mercury analyzer. From the analysis of 150 cases, 102 (68.0%) cases of fermented liquor were detected, and 35 pesticide residues (including metalaxyl, mandipropamid, azoxystrobin, and fenhexamid) were detected among the 400 pesticide residues tested. Pb, Cd, and Hg were tested in 150 samples. Lead was detected in 73 samples (48.7%), cadmium in 9 samples (6.0%), and mercury in 36 samples (24.0%). Exposure assessment was conducted to determine the safety of the detected pesticide residues and heavy metals. According to this assessment, the pesticide residues and heavy metals showed very low %ADI values (less than 1%).

Study on the Characteristics of Cultivation Period, Adaptive Genetic Resources, and Quantity for Cultivation of Rice in the Desert Environment of United Arab Emirates (United Arab Emirates 사막환경에서 벼 재배를 위한 재배기간, 유전자원 및 수량 특성 연구)

  • Jeong, Jae-Hyeok;Hwang, Woon-Ha;Lee, Hyeon-Seok;Yang, Seo-Yeong;Choi, Myoung-Goo;Kim, Jun-Hwan;Kim, Jae-Hyeon;Jung, Kang-Ho;Lee, Su-Hwan;Oh, Yang-Yeol;Lee, Kwang-Seung;Suh, Jung-Pil;Jung, Ki-Yuol;Lee, Jae-Su;Choi, In-Chan;Yu, Seung-hwa;Choi, Soon-Kun;Lee, Seul-Bi;Lee, Eun-Jin;Lee, Choung-Keun;Lee, Chung-Kuen
    • Korean Journal of Agricultural and Forest Meteorology
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    • 제24권3호
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    • pp.133-144
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    • 2022
  • This study was conducted to investigate the cultivation period, adaptive genetic resources, growth and development patterns, and water consumption for rice cultivation in the desert environment of United Arab Emirates (UAE). R esearch on rice cultivation in the desert environment is expected to contribute to resolving food shortages caused by climate change and water scarcity. It was found that the optimal cultivation period of rice was from late November to late April of the following year during which the low temperature occurred at the vegetative growth stage of rice in the UAE. Asemi and FL478 were selected to be candidate cultivars for temperature and day-length conditions in the desert areas as a result of pre-testing genetic resources under reclaimed soil and artificial meteorological conditions. In the desert environment in the UAE, FL478 died before harvest due to the etiolation and poor growth in the early stage of growth. In contrast, Asemi overcame the etiolation in the early stage of growth, which allowed for harvest. The vegetative growth phases of Asemi were from early December to early March of the following year whereas its reproductive growth and ripening phases were from early March to late March and from late March to late April, respectively. The yield of milled rice for Asemi was 763kg/10a in the UAE, which was about 41.8% higher than that in Korea. Such an outcome was likely due to the abundant solar radiation during the reproductive growth and grain filling periods. On the other hand, water consumption during the cultivation period in the UAE was 2,619 ton/10a, which was about three times higher than that in Korea. These results suggest that irrigation technology and development of cultivation methods would be needed to minimize water consumption, which would make it economically viable to grow rice in the UAE. In addition, select on of genetic resources for the UAE desert environments such as minimum etiolation in the early stages of growth would be merited further studies, which would promote stable rice cultivation in the arid conditions.

Monitoring of Pesticide Residues Concerned in Stream Water (전국 하천수 중 잔류우려 농약 실태조사)

  • Hwang, In-Seong;Oh, Yee-Jin;Kwon, Hye-Young;Ro, Jin-Ho;Kim, Dan-Bi;Moon, Byeong-Chul;Oh, Min-Seok;Noh, Hyun-Ho;Park, Sang-Won;Choi, Geun-Hyoung;Ryu, Song-Hee;Kim, Byung-Seok;Oh, Kyeong-Seok;Lim, Chi-Hwan;Lee, Hyo-Sub
    • Korean Journal of Environmental Agriculture
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    • 제38권3호
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    • pp.173-184
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    • 2019
  • BACKGROUND: This study was carried out to investigate pesticide residues from fifty streams in Korea. Water samples were collected at two times. Thee first sampling was performed from april to may, which was the season for start of pesticide application and the second sampling event was from august to september, which was a period for spraying pesticides multiple times. METHODS AND RESULTS: The 136 pesticide residues were analyzed by LC-MS/MS and GC/ECD. As a result, eleven of the pesticide residues were detected at the first sampling. Twenty eight of the pesticide residues were detected at the second sampling. Seven pesticides were frequently detected from more than 10 water samples. Ecological risk assessment (ERA) was carried out by using residual and toxicological data. Four scenarios were applied for the ERA. Scenario 1 and 2 were performed using LC50 values and mean and maximum concentrations. Scenarios 3 and 4 were conducted by NOEC values and mean and maximum concentrations. CONCLUSION: Frequently detected pesticide residues tended to coincide with the period of preventing pathogen and pest at paddy rice. As a result of ERA, five pesticides (butachlor, carbendazim, carbofuran, chlorantranilprole, and oxadiazon) were assessed to be risks at scenario 4. However, only oxadiazon was assessed to be a risk at scenario 3 for the first sampling. Oxadiazon was not assessed to be a risk at the second sampling. It seems to be temporary phenomenon at the first sampling, because usage of herbicides such as oxadiazon increased from April to march for preventing weeds at paddy fields. However, this study suggested that five pesticides which were assessed to be risks need to be monitored continuously for the residues.

Effect of Extraction Conditions on in vitro Antioxidant Activities of Root Bark Extract from Ulmus pumila L. (추출조건에 따른 유근피 추출물의 항산화 활성)

  • Kim, Jae-Min;Cho, Myoung-Lae;Seo, Kyu-Eun;Kim, Ye-Seul;Jung, Tae-Dong;Kim, Young-Hyun;Kim, Dan-Bi;Shin, Gi-Hae;Oh, Ji-Won;Lee, Jong Seok;Lee, Jin-Ha;Kim, Jong-Yae;Lee, Dae-Won;Lee, Ok-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • 제44권8호
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    • pp.1172-1179
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    • 2015
  • This study investigated optimal extraction conditions for application of Ulmus pumila L. as a natural antioxidant. U. pumila L. was extracted using ethanol (EtOH) at various concentrations (0, 40, and 80%) and extraction times (1, 2, and 3 h) at $70^{\circ}C$ and then evaluated for extraction yield, total phenolic contents, total flavonoid contents, as well as antioxidant activities [2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, reducing power, and oxygen radical absorbing capacity (ORAC)]. Antioxidant activities were correlated with total phenolic and flavonoid contents. Of the solvent conditions, 80% EtOH extracts for 3 h at $70^{\circ}C$ showed the highest total phenolic and flavonoid contents with strong antioxidant activities, although there were no significant time effects on DPPH and ABTS radical scavenging activities and reducing power. However, ORAC values of all EtOH extracts remarkably increased in a time-dependent manner. In addition, 80% EtOH extract for 3 h exhibited strong antioxidant effects on HDF and 3T3-L1 cells. Therefore, the antioxidant capacity of U. pumila L., may due to phenolic and flavonoid contents, and extraction conditions were 80% EtOH for 3 h at $70^{\circ}C$. This extract could be a good source for natural antioxidants.

Effect of Extracts from Morus alba L. and Curcuma aromatica on Shelf-life and Quality of Wet Noodle (상백피 및 강황 추출 혼합물 첨가에 따른 생면의 저장성 및 품질증진 효과)

  • Park, Na-Bi;Lee, So-Young;Yoon, So-Young;Kim, Koth-Bong-Woo-Ri;Song, Eu-Jin;Lee, So-Jeong;Lee, Chung-Jo;Jung, Ji-Yeon;Kwak, Ji-Hee;Lee, Ho-Dong;Choi, Ho-Duk;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • 제39권5호
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    • pp.750-756
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    • 2010
  • This study was performed to examine the shelf life and qualities of wet noodle with Morus alba L. root and Curcuma aromatica extracts (MCE) during storage at $4^{\circ}C$. Lightness and redness of wet noodle were decreased with increasing amounts of MCE added in noodle while yellowness was increased. The viable cell and molds count of wet noodle with MCE was reduced about 1~2 log cycle as compared with control during storage time. Also the TBARS (thiobarbituric acid reactive substance) value of wet noodle with MCE was lower than that of control. Hence the wet noodle with MCE has shown remarkable antioxidation effect. In sensory evaluation, the wet noodle containing the ratio of 2.5:0.02 of M. alba : C. aromatica was preferred than the control. From these results, the addition of 2.5% of M. alba and 0.02% of C. aromatica extracts in wet noodle had a good effect on improvement of preservation and development of quality.

A Study on the Relationship between Sleep Duration and Suicidal Idea in an Urban Area of South Korea (일 도시지역 주민들의 수면시간과 자살사고의 연관성에 관한 연구)

  • Lee, Yu-Jin;Kim, Seog-Ju;Cho, In-Hee;Kim, Jong-Hoon;Bae, Seung-Min;Koh, Seung-Hee;Cho, Seong-Jin
    • Sleep Medicine and Psychophysiology
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    • 제16권2호
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    • pp.85-90
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    • 2009
  • Introduction: There has been an increasing interest in the relationship between sleep and suicidality. In addition, suicidal patients habitually report their sleep problems. Although sleep-related complaints and electroencephalographic changes are generally encountered in psychiatric disorders, sleep complaints such as insomnia, hypersomnia and nightmares are more common in suicidal patients. In current study, we aimed at investigating the relationship between self-reported sleep duration and suicidality in general population. Methods: One thousand general population (male:female=500:500, mean age=$39.6{\pm}11.6$ years, ranged age=20-77 years) completed Center for Epidemiologic Study-Depression (CES-D), Beck Suicide Intent scale (BSI), Spielberger State-Trait Anger Expression Inventory (STAXI), Barratt Impulsiveness Scale (BIS), Morningness-Eveningness Scale (MES) and brief questionnaire of sleep habits. Results: After controlling for age and sex, score of BSI was correlated positively with the score of CES-D, STAXI and BIS on partial correlation analysis ($r_p$=0.251; p<0.001, $r_p$=0.352; p<0.001, and $r_p$=0.175; p<0.001, respectively). In addition, score of BSI was inversely correlated with the score of MES (rp=-0.066; p=0.037). However, score of BSI showed no significant correlation with sleep duration. However, regression analysis revealed that short (<6 hrs) or long (>10 hrs) sleep duration, the family history of psychiatric illness, the score of CES-D, and the score of STAXI predicted higher score of BSI significantly in total subjects (F=17.837, adjusted $R^2$=0.166; p=0.003, p=0.003, p<0.001, and p=0.003, respectively). This model was explained better in depressed subjects with 16 or higher score of CES-D (F=9.920, adjusted $R^2$=0.298). Conclusion: Current result suggested that not only short sleep duration (<6 hrs) but also long sleep duration (>10 hrs) might be related to suicidality.

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