• Restorative Dentistry and Endodontics
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• 제21권2호
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• pp.602-618
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• 1996

The purpose of this study was to investigate the effect of acid treatment of fluoride applied dentin surface with various concentrations of phosphoric acid for various periods of time on dentin bonding. Dentin specimens prepared from freshly extracted bovine mandibular anterior teeth were divided into fluoridated and nonfluoridated groups. Specimens of nonfluoridated group were pretreated with 10% phosphoric acid for 15 seconds. Those of fluoridated groups were treated with 2% sodium fluoride or 2% stannous fluoride solution for 5 minutes and stored in $37^{\circ}C$ distilled water for 3 days, followed by phosphoric acid treatment. The concentrations of phosphoric acid were 10%, 32% or 50% and the treatment periods of time were 15, 30 or 60 seconds. All the specimens were bonded with All Bond$^{(R)}$ 2 and Bisfil$^{TM}$ composite resin. After bonded specimens were stored in $37^{\circ}C$ distilled water for 24 hours, tensile bond strengths of each specimens were measured and the pretreated dentin and the fractured dentin surfaces were examined under the scanning electron microscope. The results were as follows : The tensile bond strengths from the fluoridated groups were significantly lower than those from the nonfluoridated group when the concentrations of phosphoric acid and the treatment periods of time were equal in all the groups (p<0.05). In general, the higher the concentration of phosphoric acid and the longer the treatment period of time for acid etching on the fluoride applied dentin surface, the higher were the bond strength values. Recovery of bond strength of the dentin bonding agent was better in the NaF applied group than in the $SnF_2$ applied one. SEM findings of NaF applied and $SnF_2$ applied dentin surfaces demonstrated reaction product-covered and partially or completely obstructed dentinal tubules. SEM findings of dentin surfaces fluoridated for 5 minutes followed by etching showed wider tubular openings and more clean dentin surfaces when dentin was etched with higher concentration of phosphoric acid for longer period of time. On the SEM observations of the fractured dentin-resin interface, the etched specimens of fluoridated group showed an adhesive failure mode when the concentration of phosphoric acid and the treatment period of time were same as in the nonfluoridated group. As the concentration of phosphoric acid and the treatment period of time increase during acid etching, the cohesive failure area increased. However, excessive acid etching caused adhesive failure.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2015년도 학술발표회
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• pp.336-336
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• 2015

일반적인 다목적댐은 홍수 발생 시 본댐으로 유입되는 부유쓰레기 및 잡목 등을 차단하기 위하여 댐 상류에 차단망을 설치하여 1차적으로 사전 방지하고, 홍수기 이후 선박으로 부유물을 수거하여 재활용, 소각 등으로 처리하는 시스템이다. 그러나 본 연구의 대상인 한탄강댐은 홍수조절용 댐으로서 수위 변화 폭이 매우 크고 정상 조건(비 홍수기)시는 대규모 홍수터가 드러나기 때문에 댐 유지관리 방법이 상이하며 한탄강 본류 구간은 하폭과 수심 등 하도의 불규칙성과 만곡부에서 유수의 흐름이 한쪽으로 쏠리는 편기 현상이 발생하는 등 복잡한 수리현상으로 인하여 부유쓰레기의 분포를 판단하기 어려운 지역이다. 따라서 본 연구에서는 부유쓰레기의 유동을 모의하기 위하여 HEC-HMS 모형과 $Vflo^{TM}$ 모형을 활용하여 본류와 지류의 빈도별 홍수량 분석을 실시하고, EFDC 3차원 동수역학 모형을 적용하여 홍수조절용지 내 부유쓰레기의 유입 경로 및 정체 구역을 분석하여 효과적인 쓰레기 처리 방안을 제시하고자 하였다. 분석 결과, 소규모 빈도 홍수(2년, 5년)의 경우 중 상류 지류의 부유쓰레기는 거의 유동하지 못하고 하도의 만곡의 영향을 받아 부분적으로 높은 밀도로 고착될 것으로 모의되며, 특히 본류와의 합류점 부근에 도달한 부유쓰레기는 유로 폭이 급확대되어 유속이 거의 없는 사수역 구간으로 변함에 따라 본류 배수위 영향으로 불규칙하게 맴돌다가 고착되는 것으로 모의되었다. 중규모 홍수(25년, 50년)의 경우, 상류부의 부유쓰레기의 분포는 소규모 홍수 시와 유사한 패턴을 보였으나 중류부에서 홍수파가 본류 하도를 월류하여 홍수터로 넓게 범람하는 지역에서는 소규모 홍수 시 본류 하도 양안에 분포하던 부유쓰레기가 홍수터 쪽으로 밀려가서 고착되는 경향을 보였다. 대규모 홍수(100년, 200년)의 경우, 댐과 인접한 분석 구간 하류부는 댐 배수위의 영향을 가장 크게 받으며 수심이 깊어 흐름이 가장 정체되는 구간으로 본류와 지류 모두 다량의 부유쓰레기가 분포할 것으로 분석되었다. 분석 구간 중류부는 홍수 규모가 증가함에 따라 넓은 홍수터로 침수범위가 급격하게 증가하며 이에 따라 부유쓰레기가 분포할 수 있는 면적이 크게 증가할 것으로 분석되었다. 또한 분석 구간 상류부는 협곡을 이루고 있어 홍수규모가 증가하더라도 침수범위와 부유쓰레기의 분포면적에 큰 변화가 없을 것으로 분석되었다. 본 연구 결과를 바탕으로 댐의 치수 문제가 발생하지 않도록 효과적으로 수거 처리 할 수 있는 부유쓰레기 적치장 및 차단 시설의 적정 설치 위치를 파악하였다.

• 한국토양비료학회지
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• 제40권2호
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• pp.145-150
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• 2007

간척지 논에 대한 부산석고비료 처리 효과 구명을 위하여 고흥과 영암 지역의 간척지 시범포를 대상으로 부산석고비료를 처리하여 벼의 수량, 생육 및 벼의 성분함량과 간척지 논의 토양특성에 대해 조사하였다. 출수기의 벼 초장과 주당경수는 고흥지역의 경우는 차이를 보이지 않았으나 영암지역은 초장이 111.9 cm로 무처리구보다 6.7 cm 길어 고도로 유의한 차이를 보였다. 주당경수는 처리구가 9.1개, 무처리구가 8.0개로 석고처리구가 1.1개 높게 나타났다. 부산 석고처리 유무별 벼 수량 및 성분함량은 조사지역 두 곳 모두 무처리구에 비해 처리구가 좋게 나타났다. 수량지수는 석고 처리구가 무처리구에 비해 고흥지역이 5%, 영암지역이 13% 증수되었다. 수수는 고흥지역의 무처리구가 400개 $m^{-2}$로 처리구 보다 많지만 등숙률이 처리구에서는 81%, 무처리구는 68%로 처리구에서 높게 나타났다. 미질의 성분인 단백질, 아밀로오스 및 지방산의 함량은 부산석고비료 처리구가 무처리구에 비해 높게 나타났다. 토양의 화학적 특성은 두 지역 모두 pH, EC, Av-S, Ca가 무처리구에 비해 부산석고비료 처리구에서 높게 나타났으며, 반면, 토양 중 치환성 Na는 부산석고비료 처리구가 무처리구에 비해 고흥지역 18%, 영암지역 28%로 낮아짐을 보였고, 부산석고비료를 처리함으로서 ESP와 SAR이 낮아졌다. 식물체 분석 결과 석고처리구에서 CaO > MgO > K2O의 순서로 흡수가 많아졌다.

• Investigative Magnetic Resonance Imaging
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• 제23권1호
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• pp.46-54
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• 2019

Purpose: The aim of this study was to evaluate the diagnostic performance of a computer-aided detection (CAD) system used with automated breast ultrasonography (ABUS) for suspicious lesions detected on breast MRI, and CAD-false lesions. Materials and Methods: We included a total of 40 patients diagnosed with breast cancer who underwent ABUS (ACUSON S2000) to evaluate multiple suspicious lesions found on MRI. We used CAD ($QVCAD^{TM}$) in all the ABUS examinations. We evaluated the diagnostic accuracy of CAD and analyzed the characteristics of CAD-detected lesions and the factors underlying false-positive and false-negative cases. We also analyzed false-positive lesions with CAD on ABUS. Results: Of a total of 122 suspicious lesions detected on MRI in 40 patients, we excluded 51 daughter nodules near the main breast cancer within the same quadrant and included 71 lesions. We also analyzed 23 false-positive lesions using CAD with ABUS. The sensitivity, specificity, positive predictive value, and negative predictive value of CAD (for 94 lesions) with ABUS were 75.5%, 44.4%, 59.7%, and 62.5%, respectively. CAD facilitated the detection of 81.4% (35/43) of the invasive ductal cancer and 84.9% (28/33) of the invasive ductal cancer that showed a mass (excluding non-mass). CAD also revealed 90.3% (28/31) of the invasive ductal cancers measuring larger than 1 cm (excluding non-mass and those less than 1 cm). The mean sizes of the true-positive versus false-negative mass lesions were $2.08{\pm}0.85cm$ versus $1.6{\pm}1.28cm$ (P < 0.05). False-positive lesions included sclerosing adenosis and usual ductal hyperplasia. In a total of 23 false cases of CAD, the most common (18/23) cause was marginal or subareolar shadowing, followed by three simple cysts, a hematoma, and a skin wart. Conclusion: CAD with ABUS showed promising sensitivity for the detection of invasive ductal cancer showing masses larger than 1 cm on MRI.

• 한국동물생명공학회지
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• 제34권1호
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• pp.57-63
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• 2019

This study was conducted to find out the effect that ${\kappa}-Carrageenan$ has on the properties of dog sperm when it was added to the cryoprotectant. Extender basically was contained 1.21 g Trizma base, 0.67 g citric acid, 0.4 g glucose, 0.03 g penicillin G, 0.05 g streptomycin sulfate. Extender1 was added with 0.1%, 0.2%, 0.3%, and 0.5% carrageenan, while extender2 was supplemented with glycerol. After freezing-thawing, the motility, viability, acrosome integrity, apoptosis, and ROS (reactive oxygen specifications) of sperm were measured to analyze the effects of the supplementation of carrageenan. Total Motile (TM), Rapid Progressive Motile (RPM), Medium Progressive Motile (MPM), and Immotile were measured through the CASA system after thawing in 37 degree water. Extender with 0.2% ${\kappa}-carrageenan$ ($64.26{\pm}0.49$) was significantly higher than control ($40.24{\pm}8.27$) (p < 0.05). RPMs of extender with 0.1%, 0.2% ${\kappa}-carrageenan$ ($57.64{\pm}6.34$, $56.47{\pm}1.35$) were significantly higher than the other groups (p < 0.05). Acrosome integrity was measured by dyeing to PSA-FITC with an epifluorescence microscope. Normal acrosome ratio of extender with 0.5% ${\kappa}-carrageenan$ ($61{\pm}8.03$) was higher than the other groups (p < 0.05). Apoptosis was measured with a FACSCalibur flow cytometer using FITC (FITC Annexin V Apoptosis Detection Kit). Treated groups of ${\kappa}-carrageenan$ of 0.1% ($0.81{\pm}0.05$), 0.2% ($0.85{\pm}0.05$) were significantly higer (p < 0.05) than control. Modified SYBR/PI staining was used for determination of viability and DCF staining was used for evaluation of ROS. Viability and ROS were not significantly different from other groups. In conclusion, adding a certain concentration of carrageenan to the extender of cryopreservation, carrageenan contributes to the improvement of the sperm motility, acrosome integrity and prevention of apoptosis.

• Asian Journal for Public Opinion Research
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• 제7권2호
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• pp.94-112
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• 2019

Objective: The objective of this study was to compare the quality of life (QOL) of patients with end-stage renal disease (ESRD) between patients receiving hemodialysis (HD) and patients receiving continuous ambulatory peritoneal dialysis (CAPD) and to compare personal characteristic factors relating to the quality of life in patients with ESRD. Method: This study used a descriptive research design. The sample was recruited using purposive sampling that included 76 ESRD patients receiving either HD or CAPD at a dialysis clinic in Phraphutthabat Hospital, Saraburi Province, Thailand. Data was collected using the Quality of Life Questionnaire for Chronic Kidney Disease ($KDQOL-SF^{TM}$) version 1.3. Independent t-test and ANOVA procedures were used to analyze study data. Results: The results revealed that the HD patients had a moderate level of QOL. The highest scoring dimension of QOL was the encouragement of staff at the dialysis unit and patient satisfaction with the treatment (${\bar{X}}=100$, SD=.00), followed by social support (${\bar{X}}=89.29$, SD =16.88) and cognitive function (${\bar{X}}=88.57$, SD=11.82). On the other hand, the lowest scoring QOL dimension was physical problems (${\bar{X}}=50$, SD=51.89), and pain (${\bar{X}}=50$, SD=39.03), followed by work status (${\bar{X}}=53.57$, SD=45.84) and burden from kidney disease (${\bar{X}}=58.48$, SD=31.07). The CAPD patients also had a moderate QOL. The highest scoring QOL dimension was the encouragement of staff in the renal unit and patient satisfaction with the treatment (${\bar{X}}=100$, SD=.00), followed by social support (${\bar{X}}=95.61$, SD=14.20) and cognitive function (${\bar{X}}=88.83$, SD=13.52). The worst scoring QOL dimensions were work status (${\bar{X}}=44.44$, SD=42.72), general health (${\bar{X}}=53.61$, SD=39.05), and pain (${\bar{X}}=62.70$, SD=41.14). The difference overall and in each dimension of QOL in ESRD patients who were treated with HD and CAPD was not statistically significantly different. The QOL was not significantly different among patients with different personal characteristics except for income and duration of treatment; in those cases, the difference in QOL was statistically significant (p=.05). Conclusion: The overall QOL and life expectancy of patients with ESRD treated with HD and CAPD are not affected by gender, age, marital status, education, occupation, or type of health coverage. QOL was not significantly different, except for patients with different incomes and duration of renal replacement therapy, whose QOL was significantly different. The QOL of patients receiving dialysis should be studied to develop a QOL program for patients with chronic kidney disease who receive dialysis.

• Nutrition Research and Practice
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• 제16권1호
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• pp.33-45
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• 2022

BACKGROUND/OBJECTIVES: Ginseng extract (GSE) and taurine (TR) are widely used antifatigue resources in functional foods. However, the mechanism underlying the antifatigue effects of GSE and TR are still unclear. Hence, we investigated whether GSE and TR have synergistic effects against fatigue in mice. MATERIALS/METHODS: L6 cells were treated with different concentrations of TR and GSE, and cell viability was determined using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium. Oxidative stress was analyzed by immunocytochemistry using MitoTracker^TM Red FM and an anti-8-oxoguanine antibody. Respiratory gas analysis was performed to investigate metabolism. Expression of an activated protein kinase was analyzed using immunohistochemistry. Gene expression of cluster of differentiation 36 and pyruvate dehydrogenase lipoamide kinase isozyme 4 was measured using reverse transcription-polymerase chain reaction. Mice were orally administered TR, GSE, or their combination for 30 days, and then fatigue-related parameters, including lactate, blood urea nitrogen, and glycogen, were measured after forced swimming. RESULTS: TR and GSE reduced oxidative stress levels in hydrogen peroxide-stimulated L6 cells and enhanced the oxygen uptake and lipid metabolism in mice after acute exercise. After oral administration of TR or GSE for 30 days, the fatigue-related parameters did not change in mice. However, the mice administered GSE (400 mg/kg/day) alone for 30 days could swim longer than those from the other groups. Further, no synergistic effect was observed after the swimming exercise in mice treated with the TR and GSE combination for 30 days. CONCLUSIONS: Taken together, our data suggest that TR and GSE may exert antifatigue effects in mice after acute exercise by enhancing oxygen uptake and lipid oxidation.

• The Korean Journal of Pain
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• 제35권4호
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• pp.423-432
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• 2022

Background: The decreased expression of mu-opioid receptors (MOR) in the amygdala may be a key molecular in chronic post-surgical pain (CPSP). It is known that miR-339-5p expression in the amygdala of a stressed rat model was increased. Analyzed by RNAhybrid, miR-339-5p could target opioid receptor mu 1 (oprm1) which codes MOR directly. So, the authors hypothesized that miR-339-5p could regulate the expression of MOR via targeting oprm1 and cause the effects to CPSP. Methods: To simulate perioperative short-term stress, a perioperative stress prolongs incision-induced pain hypersensitivity without changing basal pain perception rat model was built. A pmiR-RB-REPORT^TM dual luciferase assay was taken to verify whether miR-339-5p could act on oprm1 as a target. The serum glucocorticoid level of rats was test. Differential expressions of MOR, GFAP, and pERK1/2 in each group of the rats' amygdala were tested, and the expressions of miR-339-5p in each group of rats' amygdalas were also measured. Results: Perioperative stress prolonged the recovery time of incision pain. The expression of MOR was down-regulated in the amygdala of rats in stress + incision (S + IN) group significantly compared with other groups (P < 0.050). miR-339-5p was up-regulated in the amygdala of rats in group S + IN significantly compared with other groups (P < 0.050). miR-339-5p acts on oprm1 3'UTR and take MOR mRNA as a target. Conclusions: Perioperative stress could increase the expression of miR-339-5p, and miR-339-5p could cause the expression of MOR to decrease via targeting oprm1. This regulatory pathway maybe an important molecular mechanism of CPSP.

• Animal Bioscience
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• 제35권3호
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• pp.503-510
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• 2022

Objective: This study was performed to determine the effects of stress after road transportation and oral administration of chromium and meloxicam on growth performance, plasma cortisol, serum metabolites, and behavior in dairy calves. Methods: A total of 50 Holstein heifers (average body weight [BW]: 172±4.19 kg; average age: 5.53±0.12 months) were randomly assigned to five groups including NL (not transported + D-lactose; 1 mg/kg BW), TL (transported + D-lactose; 1 mg/kg BW), TC (transported + chromium; 0.5 mg/kg dry matter [DM] feed), TM (transported + meloxicam; 1 mg/kg BW), and TMC (transported + combination of meloxicam and chromium; 1 mg/kg BW and 0.5 mg/kg DM, respectively). Doses of D-lactose monohydrate, meloxicam, and chromium were prepared for oral administration by suspension in 15 mL of water in a 20-mL dosing syringe. Blood was collected before transportation, immediately after 120 km of transportation (IAT), and at 6, 24, and 48 h after transportation. Results: Neither transportation nor administration of meloxicam and/or chromium affected (p = 0.99) average daily gain and feed intake. Plasma cortisol concentrations in the NL group (average 0.13 and 0.18 nmol/L, respectively) were lower (p<0.001) compared to the TL group (average 0.39 and 0.61 nmol/L, respectively) at IAT and 48 h after transportation. At 48 h after transportation, cortisol concentrations were lower (p<0.05) in the TC group (average 0.22 nmol/L) than in the TL group (average 0.61 nmol/L), and TC calves had similar cortisol concentrations to NL calves. Lying duration (min/d) was shorter (p<0.05) in the TL group than in the NL group at 2 d after transportation. Lying duration was longer (p<0.05) for the TC and TMC groups than for the TL group at 2 d after transportation. Conclusion: Transportation increased cortisol concentrations and affected lying behavior, while chromium administration reduced cortisol concentrations and changed lying behavior. Thus, chromium administration before transportation may be a viable strategy to alleviate stress elicited by road transportation.

• 분석과학
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• 제19권1호
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• pp.107-114
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• 2006

본 연구에서는 trimethylsilyl (TMS) 및 trifluoroacetyl (TFA)유도체화를 이용하여 소변여지에서 대사이상 질환을 신속하게 스크리닝하는 GC/MS 분석법을 개발하였다. 유기산과 아미노산을 동시에 유도체화하기 위해 메틸오렌지를 지시약으로 하여 $60^{\circ}C-70^{\circ}C$에서 15-20 분간 카르복실기는 TMS로 아미노기는 TFA로 유도체한 후 GC/MS-SIM으로 분석하였다. 유기산과 아미노산의 직선성의 범위는 0.001-50 mg이었고, 소변에서의 검출한계는 10-200 ng, 정량한계는 80-900 ng 이었다. 직선성을 보이는 범위에서의 상관계수(R2)는 0.994-0.998이었고, 회수율은 methylcitric acid와 glycine을 제외하고 80-100%를 보였다. 이 방법은 기존의 유기산분석에는 GC/MS를 아미노산 분석에는 아미노산분석기를 사용하는 것과 비교할 때 GC/MS만 사용하여 동시에 유기산과 아미노산을 분석함으로써 전처리가 간단하고 짧은 분석시간과 우수한 감도, 정확도, 정밀도를 보여 주었다. 정상인과 대사이상질환 환자의 검체에 적용해 보았을 때, 본 분석방법은 유기산과 아미노산대사이상의 유전성대사질환 환자의 스크리닝, 진단 및 모니터링에 임상적으로 유용함을 보여주었다.