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Antibacterial Properties of Silver-alginate/PVP Nanofiber (은-알지네이트/PVP 나노섬유의 항균 특성)

  • Choi, Yoo-Sung;Min, Kyung-Du;Yoon, Doo-Soo
    • Applied Chemistry for Engineering
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    • v.24 no.4
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    • pp.416-422
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    • 2013
  • In order to incorporate silver ions into the alginate, silver-alginate was prepared with aqueous solutions of silver nitrate. In the study, the silver-alginate was prepared by blending with poly vinylpyrrolidone solutions and the electrospinning was performed by using this blend solution. Antibacterial properties of silver-alginate/PVP solutions were estimated for Escherichia coli and Staphylococcus aureus by the colony counting test. Electrospinning conditions of silver-alginate/PVP solution were the tip-to-collector distance of 22 cm, the flow rate of the solution at 0.01 mL/min, and the voltage at 26 kV. The form and size of silver-alginate/PVP nanofibers were estimated by SEM and Image J. The average diameter of the electrospun SA5P15 fibers was 124 nm and showed a narrow diameter distribution. The reduction of bacteria for SA5P15 exhibited 99.9% after 24 h.

3D Face Alignment and Normalization Based on Feature Detection Using Active Shape Models : Quantitative Analysis on Aligning Process (ASMs을 이용한 특징점 추출에 기반한 3D 얼굴데이터의 정렬 및 정규화 : 정렬 과정에 대한 정량적 분석)

  • Shin, Dong-Won;Park, Sang-Jun;Ko, Jae-Pil
    • Korean Journal of Computational Design and Engineering
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    • v.13 no.6
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    • pp.403-411
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    • 2008
  • The alignment of facial images is crucial for 2D face recognition. This is the same to facial meshes for 3D face recognition. Most of the 3D face recognition methods refer to 3D alignment but do not describe their approaches in details. In this paper, we focus on describing an automatic 3D alignment in viewpoint of quantitative analysis. This paper presents a framework of 3D face alignment and normalization based on feature points obtained by Active Shape Models (ASMs). The positions of eyes and mouth can give possibility of aligning the 3D face exactly in three-dimension space. The rotational transform on each axis is defined with respect to the reference position. In aligning process, the rotational transform converts an input 3D faces with large pose variations to the reference frontal view. The part of face is flopped from the aligned face using the sphere region centered at the nose tip of 3D face. The cropped face is shifted and brought into the frame with specified size for normalizing. Subsequently, the interpolation is carried to the face for sampling at equal interval and filling holes. The color interpolation is also carried at the same interval. The outputs are normalized 2D and 3D face which can be used for face recognition. Finally, we carry two sets of experiments to measure aligning errors and evaluate the performance of suggested process.

Development of Sharpness Measuring Method for Glass Aggregate Particle made from Waste Glass Bottle

  • Sano, Shigeru;Yamada, Mari
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.76-79
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    • 2001
  • In Japan in of today, there is no aspect in which the quantity of waste glass bottle discharged from ordinary homes decreases. Moreover, the kind of shape and color of the waste glass bottles are increasing little by little. In the recycling of the glass bottle, it becomes a problem that many kind of the color is abounding. It is possible to use the transparent glass bottle as raw material of the glassware. Since the reproduction of the color is difficult, the colored glass bottle is not possible to use as a raw material. Therefore, the processing of these glass bottles entirely depends on the reclamation. In Japan, the security of the new reclaimed land is very difficult for the reason of the environmental damage. Moreover, the life expectancy in many reclaimed lands is anticipated with within two years. Therefore, the development of the processing method of glass bottle other than reclamation becomes a rapid problem. At present it is examined that it is used of an aggregate of the colored pavement, as one of the application methods of colored glass bottle. The particle size of produced glass aggregate is also 5mm or less, because the thickness is 5mm or less on almost glass bottles. And almost glass particles have the sharp tip. The application as the aggregate is limited for this sharpness of the particle. This study was carried out for the purpose of the establishment of sharpness measuring method of the glass aggregate particle. It is possible that the injury degree to human in the handling is known, if showing the sharpness of the aggregate particle at the objective numerical value is possible. And the application of the glass aggregate is spread. In this research, the balloon fracture method was used for the sharpness measurement of the glass particle. This method is based on the completely new idea, and it is possible to express completely the possibility of injury to the human. It is reported with the detailed result in full paper, because this study is continuous in order to get the JIS which is industrial standard of Japan.

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Occurrence of Leptosphaerulina Leaf Blight on Kentucky Bluegrass Caused by Leptosphaerulina trifolii (Leptosphaerulina trifolii에 의한 Kentucky Bluegrass의 Leptosphaerulina 잎마름병 발생)

  • Kim, Jeong-Ho;Shim, Gyu-Yul;Kim, Young-Ho
    • Research in Plant Disease
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    • v.16 no.1
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    • pp.94-96
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    • 2010
  • In May of 2004 through 2007, Leptosphaerulina leaf blight caused by Leptosphaerulina trifolii occurred on Kentucky bluegrass (Poa pratensis) at golf courses in Gangwon Province, Korea. Symptoms on the turfgrass caused by L. trifolii were leaf blights, dying from the leaf tip downwards to the crown, which appeared patches in the field because of local pockets of severely infected (blighted) grass. Perithecia were produced on old or weak leaves, including club-shaped asci, each of which contained 8 pale brown muriform ascospores with cross and longitudinal septa. Ascospores of the fungus isolated from the diseased leaf tissue and cultured on potato-dextrose agar (PDA) were muriform multicellular (composed of 3-6 cells) and $23.4-40.5{\times}7.8-15.6{\mu}m$ in size with 3-4 transverse and 0-3 longitudinal septa, which were morphologically identical to L. trifolii reported previously. DNA sequences of ribosomal RNA gene (internal transcribed spacer) of the fungus were homologous with similarity of 99% to those of L. trifolii isolates in GenBank database, confirming the identity of the causal agent of the disease. Pathogenicity of the fungus was also confirmed on the creeping bentgrass by Koch's postulates. This is first report of Leptosphaerulina leaf blight on turfgrass caused by L. trifolii in Korea.

Comparison of Flexural Tensile Strength according to the Presence of Notch and Fiber Content in Ultra High Performance Cementitious Composites (노치 유무와 섬유혼입률에 따른 UHPCC의 휨인장강도 비교)

  • Kang, Su-Tae
    • Journal of the Korea Concrete Institute
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    • v.24 no.5
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    • pp.525-533
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    • 2012
  • In this study, bending tests were performed on beam specimens made of UHPCC with the fiber content range of 0~5 vol% to investigate the contribution of fiber content to first cracking strength and flexural tensile strength. Also, four-point bending tests for unnotched beam as well as three-point bending test for notched beam were performed to estimate the effect of the presence of notch on the strengths. The experiment result showed that the increase in fiber content made linear improvement in the flexural tensile strength; whereas first cracking strength was enhanced only when at least 1 vol% of fibers was incorporated. Comparison of the bending test results with and without notch showed that the notch effect varied with the fiber content. The increase in fiber content diminished the effect of stress concentration on the notch tip, reducing the difference in the strengths. With much higher fiber content, the effect of stress concentration almost disappeared and the defection on cracking plane or the size effect dominated the strengths, consequently resulting in higher strengths in the notched beams than the unnotched ones.

The Analysis of Fatigue Behavior Using the Delamination Growth Rate(dAD/da) and Fiber Bridging Effect Factor(FBE) in Al/GERP Laminates (층간분리성장률(dAD/da)과 섬유가교효과인자(FBE)를 이용한 Al/GFRP 적층재의 피로거동 해석)

  • Song, Sam-Hong;Kim, Cheol-Woong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.27 no.2
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    • pp.317-326
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    • 2003
  • The influence of cyclic bending moment on the delamination and the fatigue crack propagation behavior in Al/GFRP laminate such as the wing section was investigated. The main objective of this study was to evaluate the relationship between crack profile and delamination behavior. And a propose parameter on the delamination growth rate(d $A_{D}$/da) of Al/GFRP laminates with a saw-cut using relationship between delamination area( $A_{D}$) and cycles(N), crack length(a), stress intensity factor range($\Delta$K). Also, the fiber bridging effect factor( $F_{BE}$ ) was propose that the fiber bridging modification factor($\beta$$_{fb}$ ) to evaluate using the delamination growth rate(d $A_{D}$/da). The shape and size of the delamination zone formed along the fatigue crack between aluminum alloy sheet. Class fiber-adhesive layer were measured by an ultrasonic C-scan image. The shape of delamination zone turns out to be semi-elliptic with the contour decreased non-linearly toward the crack tip. It represents that relationship between crack length and delamination growth rate(d $A_{D}$/da) were interdependent by reciprocal action, therefore it's applicable present a model for the delamination growth rate(dA/sib D//da) in Al/GFRP laminates.minates.s.

Gray Mold of Safflower Caused by Botrytis cinerea (Botrytis cinerea에 의한 잇꽃 잿빛곰팡이병)

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Son, Kyung-Ae;Park, Chang-Seuk
    • The Korean Journal of Mycology
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    • v.28 no.1
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    • pp.46-48
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    • 2000
  • The gray mold disease was severely occurred on safflower grown in experimental farm of Kyongsangnam-do Agricultural Research and Extension Services in 1999. At first, the infected leaves discolored gray or dark from the tip and gradually expanded to downword. The fungus also attacked stem, pod and calyx. The infected area became dark and dried, and frequently gray mold grown on the infected area. The causal organism was isolated and proved its pathogenecity according to Koch's postulate. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and sized $7.4{\sim}16.8{\times}5.3{\times}10.4\;{\mu}m$. Conidiophores were observed with their wide size of $2.9{\times}15.5\;{\mu}m$. The temperature range for mycelial growth was between $5^{\circ}C\;and\;30^{\circ}C$ with the optimum temperatures of $20^{\circ}C\;and\;25^{\circ}C$. The causal organism was identified as Botrytis cinerea and based on mycological characteristics examined. This is the first report on gray mold of safflower caused by Botrytis cinerea in Korea.

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Mechanical and Adhesional Manipulation Technique for Micro-assembly under SEM

  • Saito, S.;Takahashi, K.;Onzawa, T.
    • International Journal of Korean Welding Society
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    • v.2 no.2
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    • pp.19-25
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    • 2002
  • In recent years, techniques for micro-assembly with high repeatability under a scanning electron microscope (SEM) are required to construct highly functional micro-devices. Adhesion phenomenon is more significant for smaller objects, because adhesional force is proportional to size of the objects while gravitational force is proportional to the third power of it. It is also known that adhesional force between micro-objects exposed to Electron Beam irradiation of SEM increases with the elapsed time. Therefore, mechanical manipulation techniques using a needle-shaped tool by adhesional force are often adopted in basic researches where micro-objects are studied. These techniques, however, have not yet achieved the desired repeatability because many of these could not have been supported theoretically. Some techniques even need the process of trial-and-error. Thus, in this paper, mechanical and adhesional micro-manipulation are analyzed theoretically by introducing new physical factors, such as adhesional force and rolling-resistance, into the kinematic system consisting of a sphere, a needle-shaped tool, and a substrate. Through this analysis, they are revealed that how the micro-sphere behavior depends on the given conditions, and that it is possible to cause the fracture of the desired contact Interfaces selectively by controlling the force direction in which the tool-tip loads to the sphere. Based on the acquired knowledge, a mode diagram, which indicates the micro-sphere behavior for the given conditions, is designed. By referring to this mode diagram, the practical technique of the pick and place manipulation of a micro-sphere under an SEM by the selective interface fracture is proposed.

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Occurrence of Gray Mold on Balsam Pear (Momordica charantia) Caused by Botrytis cinerea in Korea (Botrytis cinerea에 의한 여주 잿빛곰팡이병)

  • Kwon Jin-Hyeuk;Park Chang-Seuk
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.58-61
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    • 2006
  • Ggray mold caused by Botrytis cinerea occurred on Balsam pear (Momordica charantia) at Daesan-myon, Changwon city, Gyeongnam province in Korea from 2004 to 2006. The disease symptoms usually started with water-soaking lesions on the fruits tip and stem, and then the infected plants became withered and eventually died. The conidia of the pathogen appeared on the surface of fruit and stem of infected plants. The conidia were one celled and mostly ellipsoid or ovoid in shape and were light gray in color, The conidia were $6{\sim}20X4{\sim}12{\mu}m$ in size and conidiophores were $14{\sim}30{\mu}m$ in length. The sclerotia formed abundantly on potato-dextrose agar 18 days after incubation. The optimum temperature for sclerotial formation was $20^{\circ}C$. Pathogenicity of the causal organism was proved according to Koch,s postulates. The causal organism was identified as Botrytis cinerea Persoon: Fries based on the mycological characteristics. This is the first report on gray mold of M. charantia caused by B. cinerea in Korea.

Soft Tissue Reconstruction of Finger and Hand Using Arterialized Venous Free Flap (동맥화 정맥 유리 피판술을 이용한 수부와 수지 연부조직 결손의 재건)

  • Kong, Byeong-Seon;Kim, Yong-Jin;Cho, Kwang-Woo
    • Archives of Reconstructive Microsurgery
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    • v.13 no.2
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    • pp.107-116
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    • 2004
  • Flaps are necessary, when important structures such as bone, tendon, nerve and vessel are exposed. Arterialized venous free flap is suited to the coverage of finger and hand because the thickness of venous flap is thin. Authors performed 65 cases arterialized venous free flap for the soft tissue reconstruction of the hand and finger. The size of donor defect were from $1{\times}1cm\;to\;7{\times}12cm$. The mean flap area was $9.1cm^2$. The recipient sites were finger tip in 34 cases, finger shaft in 29 cases and hand in 2 cases. The donor sites were volar aspect of distal forearm in 40 cases, thenar area in 17 cases and foot dorsum in 6 cases. The types of arterialized venous free flap were A-A type in 4 cases and A-V type in 61 cases. The length of afferent vein was from 0.5 cm to 3 cm (mean 1.7 cm) and efferent vein was from 1 cm to 10 cm (mean 2.2 cm). 58 flaps(89.2%) survived eventually. 42 flaps(64.6%) survived totally without any complication. 8 flaps(12.3%) showed the partial necrosis but they were healed without any additional operations. 8 flaps (12.3%) showed the partial necrosis requiring the additional skin graft. We had a satisfactory result by using arterialized venous free flap for the soft tissue reconstruction of finger and hand. We believe that volar aspect of distal forearm, thenar area, foot dorsum are suited as a donor site and the short length of the flap pedicle, the strong arterail inflow affect the survival rate of arterialized venous free flaps.

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